Mycobacterium avium restriction fragment lenght polymorphism-IS IS1245 and the simple double repetitive element polymerase chain reaction typing method to screen genetic diversity in Brazilian strains
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2005 |
| Outros Autores: | , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.1590/S0074-02762005000700012 |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/44564 |
| Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Micobacterioses. Rio de Janeiro, RJ, Brasil. |
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Sequeira, Patrícia Carvalho deFonseca, Leila de SouzaSilva, Marlei Gomes daSaad, Maria Helena Féres2020-11-20T20:04:14Z2020-11-20T20:04:14Z2005SEQUEIRA. Patrícia Carvalho de et al. Mycobacterium avium restriction fragment lenght polymorphism-IS IS1245 and the simple double repetitive element polymerase chain reaction typing method to screen genetic diversity in Brazilian strains. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 100, n. 7, p. 743-748, Nov. 2005.0074-0276https://www.arca.fiocruz.br/handle/icict/4456410.1590/S0074-027620050007000121678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Mycobacterium aviumAIDSMicobactérias não TuberculosasReação em Cadeia da PolimerasePolimorfismo de Fragmento de RestriçãoIS1254-restriction fragment length polymorphismMycobacterium aviumMaDRE-polymerase chain reactionNontuberculous mycobacteriaAIDSMicobactérias não TuberculosasCélulas-TroncoPolimorfismo de Fragmento de RestriçãoMycobacterium avium restriction fragment lenght polymorphism-IS IS1245 and the simple double repetitive element polymerase chain reaction typing method to screen genetic diversity in Brazilian strainsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Micobacterioses. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Micobacterioses. Rio de Janeiro, RJ, Brasil.Simple double repetitive element polymerase chain reaction (MaDRE-PCR) and Pvu II-IS1245 restriction fragment length polymorphism (RFLP) typing methods were used to type 41 Mycobacterium avium isolates obtained from 14 AIDS inpatients and 10 environment and animals specimens identified among 53 mycobacteria isolated from 237 food, chicken, and pig. All environmental and animals strains showed orphan patterns by both methods. By MaDRE-PCR four patients, with multiple isolates, showed different patterns, suggesting polyclonal infection that was confirmed by RFLP in two of them. This first evaluation of MaDRE-PCR on Brazilian M. avium strains demonstrated that the method seems to be useful as simple and less expensive typing method for screening genetic diversity in M. avium strains on selected epidemiological studies, although with limitation on analysis identical patterns except for one band.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/44564/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALPatriciaCarvalho_Siqueira_etal_IOC_2005.pdfPatriciaCarvalho_Siqueira_etal_IOC_2005.pdfapplication/pdf3672794https://www.arca.fiocruz.br/bitstream/icict/44564/2/PatriciaCarvalho_Siqueira_etal_IOC_2005.pdf3a3095d35720b1b510d9b4fd390cec04MD52TEXTPatriciaCarvalho_Siqueira_etal_IOC_2005.pdf.txtPatriciaCarvalho_Siqueira_etal_IOC_2005.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Mycobacterium avium restriction fragment lenght polymorphism-IS IS1245 and the simple double repetitive element polymerase chain reaction typing method to screen genetic diversity in Brazilian strains |
| title |
Mycobacterium avium restriction fragment lenght polymorphism-IS IS1245 and the simple double repetitive element polymerase chain reaction typing method to screen genetic diversity in Brazilian strains |
| spellingShingle |
Mycobacterium avium restriction fragment lenght polymorphism-IS IS1245 and the simple double repetitive element polymerase chain reaction typing method to screen genetic diversity in Brazilian strains Sequeira, Patrícia Carvalho de Mycobacterium avium AIDS Micobactérias não Tuberculosas Reação em Cadeia da Polimerase Polimorfismo de Fragmento de Restrição IS1254-restriction fragment length polymorphism Mycobacterium avium MaDRE-polymerase chain reaction Nontuberculous mycobacteria AIDS Micobactérias não Tuberculosas Células-Tronco Polimorfismo de Fragmento de Restrição |
| title_short |
Mycobacterium avium restriction fragment lenght polymorphism-IS IS1245 and the simple double repetitive element polymerase chain reaction typing method to screen genetic diversity in Brazilian strains |
| title_full |
Mycobacterium avium restriction fragment lenght polymorphism-IS IS1245 and the simple double repetitive element polymerase chain reaction typing method to screen genetic diversity in Brazilian strains |
| title_fullStr |
Mycobacterium avium restriction fragment lenght polymorphism-IS IS1245 and the simple double repetitive element polymerase chain reaction typing method to screen genetic diversity in Brazilian strains |
| title_full_unstemmed |
Mycobacterium avium restriction fragment lenght polymorphism-IS IS1245 and the simple double repetitive element polymerase chain reaction typing method to screen genetic diversity in Brazilian strains |
| title_sort |
Mycobacterium avium restriction fragment lenght polymorphism-IS IS1245 and the simple double repetitive element polymerase chain reaction typing method to screen genetic diversity in Brazilian strains |
| author |
Sequeira, Patrícia Carvalho de |
| author_facet |
Sequeira, Patrícia Carvalho de Fonseca, Leila de Souza Silva, Marlei Gomes da Saad, Maria Helena Féres |
| author_role |
author |
| author2 |
Fonseca, Leila de Souza Silva, Marlei Gomes da Saad, Maria Helena Féres |
| author2_role |
author author author |
| dc.contributor.author.fl_str_mv |
Sequeira, Patrícia Carvalho de Fonseca, Leila de Souza Silva, Marlei Gomes da Saad, Maria Helena Féres |
| dc.subject.other.pt_BR.fl_str_mv |
Mycobacterium avium AIDS Micobactérias não Tuberculosas Reação em Cadeia da Polimerase Polimorfismo de Fragmento de Restrição |
| topic |
Mycobacterium avium AIDS Micobactérias não Tuberculosas Reação em Cadeia da Polimerase Polimorfismo de Fragmento de Restrição IS1254-restriction fragment length polymorphism Mycobacterium avium MaDRE-polymerase chain reaction Nontuberculous mycobacteria AIDS Micobactérias não Tuberculosas Células-Tronco Polimorfismo de Fragmento de Restrição |
| dc.subject.en.pt_BR.fl_str_mv |
IS1254-restriction fragment length polymorphism Mycobacterium avium MaDRE-polymerase chain reaction Nontuberculous mycobacteria AIDS |
| dc.subject.decs.pt_BR.fl_str_mv |
Micobactérias não Tuberculosas Células-Tronco Polimorfismo de Fragmento de Restrição |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Micobacterioses. Rio de Janeiro, RJ, Brasil. |
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2005 |
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2005 |
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2020-11-20T20:04:14Z |
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2020-11-20T20:04:14Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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SEQUEIRA. Patrícia Carvalho de et al. Mycobacterium avium restriction fragment lenght polymorphism-IS IS1245 and the simple double repetitive element polymerase chain reaction typing method to screen genetic diversity in Brazilian strains. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 100, n. 7, p. 743-748, Nov. 2005. |
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https://www.arca.fiocruz.br/handle/icict/44564 |
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0074-0276 |
| dc.identifier.doi.none.fl_str_mv |
10.1590/S0074-02762005000700012 |
| dc.identifier.eissn.none.fl_str_mv |
1678-8060 |
| identifier_str_mv |
SEQUEIRA. Patrícia Carvalho de et al. Mycobacterium avium restriction fragment lenght polymorphism-IS IS1245 and the simple double repetitive element polymerase chain reaction typing method to screen genetic diversity in Brazilian strains. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 100, n. 7, p. 743-748, Nov. 2005. 0074-0276 10.1590/S0074-02762005000700012 1678-8060 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. |
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