DNA barcoding does not separate South American Triatoma (Hemiptera: Reduviidae), Chagas Disease vectors

Detalhes bibliográficos
Autor(a) principal: Justi, Silvia Andrade
Data de Publicação: 2014
Outros Autores: Dale, Caroline, Galvão, Cleber
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10886
Resumo: Universidade Federal do Rio de Janeiro. Instituto de Biologia. Laboratório de Biologia Evolutiva Teórica e Aplicada.Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos. Rio de Janeiro, RJ, Brasil.
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spelling Justi, Silvia AndradeDale, CarolineGalvão, Cleber2015-06-17T12:05:26Z2015-06-17T12:05:26Z2014JUSTI, Silvia Andrade; DALE, Carolina; GALVÃO, Cleber. DNA barcoding does not separate South American Triatoma (Hemiptera: Reduviidae), Chagas Disease vectors. Parasites & Vectors, v.7:519, 4p, 2014.1756-3305https://www.arca.fiocruz.br/handle/icict/1088610.1186/s13071-014-0519-1engBioMed CentralDoença de ChagasTriatominaeTriatominaeChagas diseaseDNA barcodingMolecular identificationDNA barcoding does not separate South American Triatoma (Hemiptera: Reduviidae), Chagas Disease vectorsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Biologia. Laboratório de Biologia Evolutiva Teórica e Aplicada.Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos. Rio de Janeiro, RJ, Brasil.Background: DNA barcoding assumes that a biological entity is completely separated from its closest relatives by a barcoding gap, which means that intraspecific genetic distance (from COI sequences) should never be greater than interspecific distances. We investigated the applicability of this strategy in identifying species of the genus Triatoma from South America. Findings: We calculated intra and interspecific Kimura-2-parameter distances between species from the infestans, matogrossensis, sordida and rubrovaria subcomplexes. In every subcomplex examined we observed at least one intraspecific distance greater than interspecific distances. Conclusions: Although DNA barcoding is a straightforward approach, it was not applicable for identifying Southern American Triatoma species, which may have diverged recently. Thus, caution should be taken in identifying vector species using this approach, especially in groups where accurate identification of taxa is fundamentally linked to public health issues.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10886/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALcarolina_daleetal_IOC_2014.pdfapplication/pdf160608https://www.arca.fiocruz.br/bitstream/icict/10886/2/carolina_daleetal_IOC_2014.pdf00da6412cf067887fee20c6f5984f87cMD52TEXTcarolina_daleetal_IOC_2014.pdf.txtcarolina_daleetal_IOC_2014.pdf.txtExtracted texttext/plain0https://www.arca.fiocruz.br/bitstream/icict/10886/3/carolina_daleetal_IOC_2014.pdf.txtd41d8cd98f00b204e9800998ecf8427eMD53icict/108862022-06-24 12:19:00.063oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T15:19Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv DNA barcoding does not separate South American Triatoma (Hemiptera: Reduviidae), Chagas Disease vectors
title DNA barcoding does not separate South American Triatoma (Hemiptera: Reduviidae), Chagas Disease vectors
spellingShingle DNA barcoding does not separate South American Triatoma (Hemiptera: Reduviidae), Chagas Disease vectors
Justi, Silvia Andrade
Doença de Chagas
Triatominae
Triatominae
Chagas disease
DNA barcoding
Molecular identification
title_short DNA barcoding does not separate South American Triatoma (Hemiptera: Reduviidae), Chagas Disease vectors
title_full DNA barcoding does not separate South American Triatoma (Hemiptera: Reduviidae), Chagas Disease vectors
title_fullStr DNA barcoding does not separate South American Triatoma (Hemiptera: Reduviidae), Chagas Disease vectors
title_full_unstemmed DNA barcoding does not separate South American Triatoma (Hemiptera: Reduviidae), Chagas Disease vectors
title_sort DNA barcoding does not separate South American Triatoma (Hemiptera: Reduviidae), Chagas Disease vectors
author Justi, Silvia Andrade
author_facet Justi, Silvia Andrade
Dale, Caroline
Galvão, Cleber
author_role author
author2 Dale, Caroline
Galvão, Cleber
author2_role author
author
dc.contributor.author.fl_str_mv Justi, Silvia Andrade
Dale, Caroline
Galvão, Cleber
dc.subject.other.pt_BR.fl_str_mv Doença de Chagas
Triatominae
topic Doença de Chagas
Triatominae
Triatominae
Chagas disease
DNA barcoding
Molecular identification
dc.subject.en.pt_BR.fl_str_mv Triatominae
Chagas disease
DNA barcoding
Molecular identification
description Universidade Federal do Rio de Janeiro. Instituto de Biologia. Laboratório de Biologia Evolutiva Teórica e Aplicada.Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos. Rio de Janeiro, RJ, Brasil.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-06-17T12:05:26Z
dc.date.available.fl_str_mv 2015-06-17T12:05:26Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv JUSTI, Silvia Andrade; DALE, Carolina; GALVÃO, Cleber. DNA barcoding does not separate South American Triatoma (Hemiptera: Reduviidae), Chagas Disease vectors. Parasites & Vectors, v.7:519, 4p, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10886
dc.identifier.issn.pt_BR.fl_str_mv 1756-3305
dc.identifier.doi.pt_BR.fl_str_mv 10.1186/s13071-014-0519-1
identifier_str_mv JUSTI, Silvia Andrade; DALE, Carolina; GALVÃO, Cleber. DNA barcoding does not separate South American Triatoma (Hemiptera: Reduviidae), Chagas Disease vectors. Parasites & Vectors, v.7:519, 4p, 2014.
1756-3305
10.1186/s13071-014-0519-1
url https://www.arca.fiocruz.br/handle/icict/10886
dc.language.iso.fl_str_mv eng
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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