Impaired innate immunity in Tlr4(-/-) mice but preserved CD8+ T cell responses against Trypanosoma cruzi in Tlr4-, Tlr2-, Tlr9- or Myd88-deficient mice

Detalhes bibliográficos
Autor(a) principal: Oliveira, Ana Carolina
Data de Publicação: 2010
Outros Autores: Alencar, Bruna C. de, Tzelepis, Fanny, Klezewsky, Weberton, Silva, Raquel N. da, Neves, Fabieni S., Cavalcanti, Gisele S., Boscardin, Silvia, Nunes, Marise P, Santiago, Marcelo F., Nóbrega, Alberto, Rodrigues, Maurício M., Bellio, Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/26387
Resumo: Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Rio de Janeiro, RJ, Brasil.
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spelling Oliveira, Ana CarolinaAlencar, Bruna C. deTzelepis, FannyKlezewsky, WebertonSilva, Raquel N. daNeves, Fabieni S.Cavalcanti, Gisele S.Boscardin, SilviaNunes, Marise PSantiago, Marcelo F.Nóbrega, AlbertoRodrigues, Maurício M.Bellio, Maria2018-05-10T16:25:42Z2018-05-10T16:25:42Z2010OLIVEIRA, Ana Carolina; et al. Impaired Innate Immunity in Tlr42/2 Mice but Preserved CD8+ T Cell Responses against Trypanosoma cruzi in Tlr4-, Tlr2-, Tlr9- or Myd88-Deficient Mice. Plos Pathogens, v.6, n.4, e1000870, 16p, Apr. 2010.1553-7366https://www.arca.fiocruz.br/handle/icict/2638710.1371/journal.ppat.10008701553-7374engPublic Library of ScienceTrypanosoma cruzimodelo murinoImunidade InataRespostas de células T CD8InfecçãoTrypanosoma cruziInnate ImmunityCD8+ T Cell Responsesmurine modelinfectionImpaired innate immunity in Tlr4(-/-) mice but preserved CD8+ T cell responses against Trypanosoma cruzi in Tlr4-, Tlr2-, Tlr9- or Myd88-deficient miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Rio de Janeiro, RJ, Brasil.Universidade Federal de São Paulo. Centro Interdisciplinar de Terapia Gênica. São Paulo, SP, Brasil.Universidade Federal de São Paulo. Centro Interdisciplinar de Terapia Gênica. São Paulo, SP, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Rio de Janeiro, RJ, Brasil.Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Rio de Janeiro, RJ, Brasil.Universidade Federal de São Paulo. Centro Interdisciplinar de Terapia Gênica. São Paulo, SP, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Rio de Janeiro, RJ, Brasil.The murine model of T. cruzi infection has provided compelling evidence that development of host resistance against intracellular protozoans critically depends on the activation of members of the Toll-like receptor (TLR) family via the MyD88 adaptor molecule. However, the possibility that TLR/MyD88 signaling pathways also control the induction of immunoprotective CD8+ T cell-mediated effector functions has not been investigated to date. We addressed this question by measuring the frequencies of IFN-gamma secreting CD8+ T cells specific for H-2K(b)-restricted immunodominant peptides as well as the in vivo Ag-specific cytotoxic response in infected animals that are deficient either in TLR2, TLR4, TLR9 or MyD88 signaling pathways. Strikingly, we found that T. cruzi-infected Tlr2(-/-), Tlr4(-/-), Tlr9(-/) (-) or Myd88(-/-) mice generated both specific cytotoxic responses and IFN-gamma secreting CD8+ T cells at levels comparable to WT mice, although the frequency of IFN-gamma+CD4+ cells was diminished in infected Myd88(-/-) mice. We also analyzed the efficiency of TLR4-driven immune responses against T. cruzi using TLR4-deficient mice on the C57BL genetic background (B6 and B10). Our studies demonstrated that TLR4 signaling is required for optimal production of IFN-gamma, TNF-alpha and nitric oxide (NO) in the spleen of infected animals and, as a consequence, Tlr4(-/-) mice display higher parasitemia levels. Collectively, our results indicate that TLR4, as well as previously shown for TLR2, TLR9 and MyD88, contributes to the innate immune response and, consequently, resistance in the acute phase of infection, although each of these pathways is not individually essential for the generation of class I-restricted responses against T. cruzi.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/26387/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALmarise2_nunes_etal_IOC_2010.pdfmarise2_nunes_etal_IOC_2010.pdfapplication/pdf1630536https://www.arca.fiocruz.br/bitstream/icict/26387/2/marise2_nunes_etal_IOC_2010.pdf164199248f3ab82fe3340901bb424153MD52TEXTmarise2_nunes_etal_IOC_2010.pdf.txtmarise2_nunes_etal_IOC_2010.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Impaired innate immunity in Tlr4(-/-) mice but preserved CD8+ T cell responses against Trypanosoma cruzi in Tlr4-, Tlr2-, Tlr9- or Myd88-deficient mice
title Impaired innate immunity in Tlr4(-/-) mice but preserved CD8+ T cell responses against Trypanosoma cruzi in Tlr4-, Tlr2-, Tlr9- or Myd88-deficient mice
spellingShingle Impaired innate immunity in Tlr4(-/-) mice but preserved CD8+ T cell responses against Trypanosoma cruzi in Tlr4-, Tlr2-, Tlr9- or Myd88-deficient mice
Oliveira, Ana Carolina
Trypanosoma cruzi
modelo murino
Imunidade Inata
Respostas de células T CD8
Infecção
Trypanosoma cruzi
Innate Immunity
CD8+ T Cell Responses
murine model
infection
title_short Impaired innate immunity in Tlr4(-/-) mice but preserved CD8+ T cell responses against Trypanosoma cruzi in Tlr4-, Tlr2-, Tlr9- or Myd88-deficient mice
title_full Impaired innate immunity in Tlr4(-/-) mice but preserved CD8+ T cell responses against Trypanosoma cruzi in Tlr4-, Tlr2-, Tlr9- or Myd88-deficient mice
title_fullStr Impaired innate immunity in Tlr4(-/-) mice but preserved CD8+ T cell responses against Trypanosoma cruzi in Tlr4-, Tlr2-, Tlr9- or Myd88-deficient mice
title_full_unstemmed Impaired innate immunity in Tlr4(-/-) mice but preserved CD8+ T cell responses against Trypanosoma cruzi in Tlr4-, Tlr2-, Tlr9- or Myd88-deficient mice
title_sort Impaired innate immunity in Tlr4(-/-) mice but preserved CD8+ T cell responses against Trypanosoma cruzi in Tlr4-, Tlr2-, Tlr9- or Myd88-deficient mice
author Oliveira, Ana Carolina
author_facet Oliveira, Ana Carolina
Alencar, Bruna C. de
Tzelepis, Fanny
Klezewsky, Weberton
Silva, Raquel N. da
Neves, Fabieni S.
Cavalcanti, Gisele S.
Boscardin, Silvia
Nunes, Marise P
Santiago, Marcelo F.
Nóbrega, Alberto
Rodrigues, Maurício M.
Bellio, Maria
author_role author
author2 Alencar, Bruna C. de
Tzelepis, Fanny
Klezewsky, Weberton
Silva, Raquel N. da
Neves, Fabieni S.
Cavalcanti, Gisele S.
Boscardin, Silvia
Nunes, Marise P
Santiago, Marcelo F.
Nóbrega, Alberto
Rodrigues, Maurício M.
Bellio, Maria
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira, Ana Carolina
Alencar, Bruna C. de
Tzelepis, Fanny
Klezewsky, Weberton
Silva, Raquel N. da
Neves, Fabieni S.
Cavalcanti, Gisele S.
Boscardin, Silvia
Nunes, Marise P
Santiago, Marcelo F.
Nóbrega, Alberto
Rodrigues, Maurício M.
Bellio, Maria
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
modelo murino
Imunidade Inata
Respostas de células T CD8
Infecção
topic Trypanosoma cruzi
modelo murino
Imunidade Inata
Respostas de células T CD8
Infecção
Trypanosoma cruzi
Innate Immunity
CD8+ T Cell Responses
murine model
infection
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Innate Immunity
CD8+ T Cell Responses
murine model
infection
description Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Rio de Janeiro, RJ, Brasil.
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2018-05-10T16:25:42Z
dc.date.available.fl_str_mv 2018-05-10T16:25:42Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv OLIVEIRA, Ana Carolina; et al. Impaired Innate Immunity in Tlr42/2 Mice but Preserved CD8+ T Cell Responses against Trypanosoma cruzi in Tlr4-, Tlr2-, Tlr9- or Myd88-Deficient Mice. Plos Pathogens, v.6, n.4, e1000870, 16p, Apr. 2010.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/26387
dc.identifier.issn.pt_BR.fl_str_mv 1553-7366
dc.identifier.doi.none.fl_str_mv 10.1371/journal.ppat.1000870
dc.identifier.eissn.none.fl_str_mv 1553-7374
identifier_str_mv OLIVEIRA, Ana Carolina; et al. Impaired Innate Immunity in Tlr42/2 Mice but Preserved CD8+ T Cell Responses against Trypanosoma cruzi in Tlr4-, Tlr2-, Tlr9- or Myd88-Deficient Mice. Plos Pathogens, v.6, n.4, e1000870, 16p, Apr. 2010.
1553-7366
10.1371/journal.ppat.1000870
1553-7374
url https://www.arca.fiocruz.br/handle/icict/26387
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/26387/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/26387/2/marise2_nunes_etal_IOC_2010.pdf
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