Crystal structure of Jararacussin-I: The highly negatively charged catalytic interface contributes to macromolecular selectivity in snake venom thrombin-like enzymes

Detalhes bibliográficos
Autor(a) principal: Ullah, Anwar
Data de Publicação: 2012
Outros Autores: Souza, Tatiana de Arruda Campos Brasil de, Zanphorlin, Leticia Maria, Mariutti, Ricardo B., Santana, V. S., Murakami, Mario Tyago, Arni, R. K.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/15602
Resumo: FAPESP, CNPq, TWAS, DAAD, CAPES
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spelling Ullah, AnwarSouza, Tatiana de Arruda Campos Brasil deZanphorlin, Leticia MariaMariutti, Ricardo B.Santana, V. S.Murakami, Mario TyagoArni, R. K.2016-09-02T19:10:05Z2016-09-02T19:10:05Z2012ULLAH, Anwar et al. Crystal structure of Jararacussin-I: The highly negatively charged catalytic interface contributes to macromolecular selectivity in snake venom thrombin-like enzymes. Protein Science, v. 22, n. 1, p. 128-132, 2012.0961-8368https://www.arca.fiocruz.br/handle/icict/1560210.1002/pro.2189FAPESP, CNPq, TWAS, DAAD, CAPESUniversidade Estadual Paulista. Instituto de Biociências Letras e Ciências Exatas. Departamento de Física. Centro Multiusuário de Inovação Biomolecular, São Jose do Rio Preto, SP, Brasil.Centro Nacional de Pesquisa em Energia e Materiais. Laboratório Nacional de Biociências. Campinas, SP, Brasil / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Universidade Estadual Paulista. Instituto de Biociências Letras e Ciências Exatas. Departamento de Física. Centro Multiusuário de Inovação Biomolecular, São Jose do Rio Preto, SP, Brasil.Universidade Estadual Paulista. Instituto de Biociências Letras e Ciências Exatas. Departamento de Física. Centro Multiusuário de Inovação Biomolecular, São Jose do Rio Preto, SP, Brasil.Universidade Estadual Paulista. Instituto de Biociências Letras e Ciências Exatas. Departamento de Física. Centro Multiusuário de Inovação Biomolecular, São Jose do Rio Preto, SP, Brasil.Centro Nacional de Pesquisa em Energia e Materiais. Laboratório Nacional de Biociências. Campinas, SP, Brasil.Universidade Estadual Paulista. Instituto de Biociências Letras e Ciências Exatas. Departamento de Física. Centro Multiusuário de Inovação Biomolecular, São Jose do Rio Preto, SP, Brasil.Snake venom serine proteinases (SVSPs) are hemostatically active toxins that perturb the maintenance and regulation of both the blood coagulation cascade and fibrinolytic feedback system at specific points, and hence, are widely used as tools in pharmacological and clinical diagnosis. The crystal structure of a thrombin-like enzyme (TLE) from Bothrops jararacussu venom (Jararacussin-I) was determined at 2.48 Å resolution. This is the first crystal structure of a TLE and allows structural comparisons with both the Agkistrodon contortrix contortrix Protein C Activator and the Trimeresurus stejnegeri plasminogen activator. Despite the highly conserved overall fold, significant differences in the amino acid compositions and three-dimensional conformations of the loops surrounding the active site significantly alter the molecular topography and charge distribution profile of the catalytic interface. In contrast to other SVSPs, the catalytic interface of Jararacussin-I is highly negatively charged, which contributes to its unique macromolecular selectivity.porCrystal structure of Jararacussin-I: The highly negatively charged catalytic interface contributes to macromolecular selectivity in snake venom thrombin-like enzymesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleJararacussin-IThrombin-like enzymeBothrops jararacussuCrystal structureProteinsProteínasReceptores de TrombinaVenenos de Serpentesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83074https://www.arca.fiocruz.br/bitstream/icict/15602/1/license.txt0271fb68963c017aa933c7aff60379f4MD51ORIGINALCrystal structure of Jararacussin-I.pdfCrystal structure of Jararacussin-I.pdfapplication/pdf356808https://www.arca.fiocruz.br/bitstream/icict/15602/2/Crystal%20structure%20of%20Jararacussin-I.pdfcd01e76570df32e71244d77604645768MD52TEXTCrystal structure of Jararacussin-I.pdf.txtCrystal structure of Jararacussin-I.pdf.txtExtracted texttext/plain20677https://www.arca.fiocruz.br/bitstream/icict/15602/3/Crystal%20structure%20of%20Jararacussin-I.pdf.txt23cb3194dcccd9ee15466b5b34b5b42fMD53icict/156022019-11-23 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dc.title.pt_BR.fl_str_mv Crystal structure of Jararacussin-I: The highly negatively charged catalytic interface contributes to macromolecular selectivity in snake venom thrombin-like enzymes
title Crystal structure of Jararacussin-I: The highly negatively charged catalytic interface contributes to macromolecular selectivity in snake venom thrombin-like enzymes
spellingShingle Crystal structure of Jararacussin-I: The highly negatively charged catalytic interface contributes to macromolecular selectivity in snake venom thrombin-like enzymes
Ullah, Anwar
Jararacussin-I
Thrombin-like enzyme
Bothrops jararacussu
Crystal structure
Proteins
Proteínas
Receptores de Trombina
Venenos de Serpentes
title_short Crystal structure of Jararacussin-I: The highly negatively charged catalytic interface contributes to macromolecular selectivity in snake venom thrombin-like enzymes
title_full Crystal structure of Jararacussin-I: The highly negatively charged catalytic interface contributes to macromolecular selectivity in snake venom thrombin-like enzymes
title_fullStr Crystal structure of Jararacussin-I: The highly negatively charged catalytic interface contributes to macromolecular selectivity in snake venom thrombin-like enzymes
title_full_unstemmed Crystal structure of Jararacussin-I: The highly negatively charged catalytic interface contributes to macromolecular selectivity in snake venom thrombin-like enzymes
title_sort Crystal structure of Jararacussin-I: The highly negatively charged catalytic interface contributes to macromolecular selectivity in snake venom thrombin-like enzymes
author Ullah, Anwar
author_facet Ullah, Anwar
Souza, Tatiana de Arruda Campos Brasil de
Zanphorlin, Leticia Maria
Mariutti, Ricardo B.
Santana, V. S.
Murakami, Mario Tyago
Arni, R. K.
author_role author
author2 Souza, Tatiana de Arruda Campos Brasil de
Zanphorlin, Leticia Maria
Mariutti, Ricardo B.
Santana, V. S.
Murakami, Mario Tyago
Arni, R. K.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ullah, Anwar
Souza, Tatiana de Arruda Campos Brasil de
Zanphorlin, Leticia Maria
Mariutti, Ricardo B.
Santana, V. S.
Murakami, Mario Tyago
Arni, R. K.
dc.subject.en.pt_BR.fl_str_mv Jararacussin-I
Thrombin-like enzyme
Bothrops jararacussu
Crystal structure
topic Jararacussin-I
Thrombin-like enzyme
Bothrops jararacussu
Crystal structure
Proteins
Proteínas
Receptores de Trombina
Venenos de Serpentes
dc.subject.decs.En.fl_str_mv Proteins
dc.subject.decs.pt_BR.fl_str_mv Proteínas
Receptores de Trombina
Venenos de Serpentes
description FAPESP, CNPq, TWAS, DAAD, CAPES
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2016-09-02T19:10:05Z
dc.date.available.fl_str_mv 2016-09-02T19:10:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ULLAH, Anwar et al. Crystal structure of Jararacussin-I: The highly negatively charged catalytic interface contributes to macromolecular selectivity in snake venom thrombin-like enzymes. Protein Science, v. 22, n. 1, p. 128-132, 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/15602
dc.identifier.issn.pt_BR.fl_str_mv 0961-8368
dc.identifier.doi.pt_BR.fl_str_mv 10.1002/pro.2189
identifier_str_mv ULLAH, Anwar et al. Crystal structure of Jararacussin-I: The highly negatively charged catalytic interface contributes to macromolecular selectivity in snake venom thrombin-like enzymes. Protein Science, v. 22, n. 1, p. 128-132, 2012.
0961-8368
10.1002/pro.2189
url https://www.arca.fiocruz.br/handle/icict/15602
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/15602/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/15602/2/Crystal%20structure%20of%20Jararacussin-I.pdf
https://www.arca.fiocruz.br/bitstream/icict/15602/3/Crystal%20structure%20of%20Jararacussin-I.pdf.txt
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