IFNG +874T/A polymorphism is not associated with American tegumentary leishmaniasis susceptibility but can influence Leishmania induced IFN-gamma production
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2007 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/39929 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Micobacterioses. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. |
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Matos, Guilherme InocêncioCovas, Claudia de J. FernandesBittar, Rita de CássiaSilva, Adriano GomesMarques, FabianaManiero, Viviane C.Amato, Valdir S.Oliveira Neto, Manoel P.Mattos, Marise da SilvaPirmez, ClaudeSampaio, Elizabeth P.Moraes, Milton O.Cruz, Alda Maria da2020-02-13T16:36:49Z2020-02-13T16:36:49Z2007MATOS, Guilherme Inocêncio et al. IFNG +874T/A polymorphism is not associated with American tegumentary leishmaniasis susceptibility but can influence Leishmania induced IFN-γ production. BMC Infectious Diseases, v. 7, n. 33, p. 1-6, 2007.1471-2334https://www.arca.fiocruz.br/handle/icict/3992910.1186/1471-2334-7-33engBioMed CentralLeishmaniaLeishmaniose tegumentar americanaGenes reguladores polimórficosAmerican tegumentary leishmaniasisLeishmaniaIFN-γ productionIFNG +874T/A polymorphismPolymorphic regulatory genesIFNG +874T/A polymorphism is not associated with American tegumentary leishmaniasis susceptibility but can influence Leishmania induced IFN-gamma productioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Micobacterioses. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Micobacterioses. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Universidade de São Paulo. Faculdade de Medicina. Divisão de Clínica de Moléstias Infecciosas e Parasitárias. Ambulatório de Leishmanioses. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Imunopatologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Micobacterioses. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Micobacterioses. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Background: Interferon-gamma is a key cytokine in the protective responses against intracellular pathogens. A single nucleotide polymorphism (SNP) located in the first intron of the human IFN-γ gene can putatively influence the secretion of cytokine with an impact on infection outcome as demonstrated for tuberculosis and other complex diseases. Our aim was to investigate the putative association of IFNG+874T/A SNP with American tegumentary leishmaniasis (ATL) and also the influence of this SNP in the secretion of IFN-γ in vitro. Methods: Brazilian ATL patients (78 cutaneous, CL, and 58 mucosal leishmaniasis, ML) and 609 healthy volunteers were evaluated. The genotype of +874 region in the IFN-γ gene was carried out by Amplification Refractory Mutational System (ARMS-PCR). Leishmania-induced IFN-γ production on peripheral blood mononuclear cell (PBMC) culture supernatants was assessed by ELISA. Results: There are no differences between +874T/A SNP frequency in cases and controls or in ML versus CL patients. Cutaneous leishmaniasis cases exhibiting AA genotype produced lower levels of IFN-γ than TA/TT genotypes. In mucosal cases, high and low IFN-γ producers were clearly demonstrated but no differences in the cytokine production was observed among the IFNG +874T or A carriers. Conclusion: Our results suggest that +874T/A polymorphism was not associated with either susceptibility or severity to leishmaniasis. Despite this, IFNG +874T/A SNP could be involved in the pathogenesis of leishmaniasis by influencing the amount of cytokine released by CL patients, although it could not prevent disease development. On the other hand, it is possible that in ML cases, other potential polymorphic regulatory genes such as TNF-α and IL-10 are also involved thus interfering with IFN-γ secretion.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/39929/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMiltonOMoraes_AldaMCruz_etal_IOC_2007.pdfMiltonOMoraes_AldaMCruz_etal_IOC_2007.pdfapplication/pdf274996https://www.arca.fiocruz.br/bitstream/icict/39929/2/MiltonOMoraes_AldaMCruz_etal_IOC_2007.pdfc1e2d5431153a91a02f0f2c717576f7dMD52TEXTMiltonOMoraes_AldaMCruz_etal_IOC_2007.pdf.txtMiltonOMoraes_AldaMCruz_etal_IOC_2007.pdf.txtExtracted texttext/plain27731https://www.arca.fiocruz.br/bitstream/icict/39929/3/MiltonOMoraes_AldaMCruz_etal_IOC_2007.pdf.txt9487fd9e1bd284b5648bce4fb734a390MD53icict/399292020-02-17 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| dc.title.pt_BR.fl_str_mv |
IFNG +874T/A polymorphism is not associated with American tegumentary leishmaniasis susceptibility but can influence Leishmania induced IFN-gamma production |
| title |
IFNG +874T/A polymorphism is not associated with American tegumentary leishmaniasis susceptibility but can influence Leishmania induced IFN-gamma production |
| spellingShingle |
IFNG +874T/A polymorphism is not associated with American tegumentary leishmaniasis susceptibility but can influence Leishmania induced IFN-gamma production Matos, Guilherme Inocêncio Leishmania Leishmaniose tegumentar americana Genes reguladores polimórficos American tegumentary leishmaniasis Leishmania IFN-γ production IFNG +874T/A polymorphism Polymorphic regulatory genes |
| title_short |
IFNG +874T/A polymorphism is not associated with American tegumentary leishmaniasis susceptibility but can influence Leishmania induced IFN-gamma production |
| title_full |
IFNG +874T/A polymorphism is not associated with American tegumentary leishmaniasis susceptibility but can influence Leishmania induced IFN-gamma production |
| title_fullStr |
IFNG +874T/A polymorphism is not associated with American tegumentary leishmaniasis susceptibility but can influence Leishmania induced IFN-gamma production |
| title_full_unstemmed |
IFNG +874T/A polymorphism is not associated with American tegumentary leishmaniasis susceptibility but can influence Leishmania induced IFN-gamma production |
| title_sort |
IFNG +874T/A polymorphism is not associated with American tegumentary leishmaniasis susceptibility but can influence Leishmania induced IFN-gamma production |
| author |
Matos, Guilherme Inocêncio |
| author_facet |
Matos, Guilherme Inocêncio Covas, Claudia de J. Fernandes Bittar, Rita de Cássia Silva, Adriano Gomes Marques, Fabiana Maniero, Viviane C. Amato, Valdir S. Oliveira Neto, Manoel P. Mattos, Marise da Silva Pirmez, Claude Sampaio, Elizabeth P. Moraes, Milton O. Cruz, Alda Maria da |
| author_role |
author |
| author2 |
Covas, Claudia de J. Fernandes Bittar, Rita de Cássia Silva, Adriano Gomes Marques, Fabiana Maniero, Viviane C. Amato, Valdir S. Oliveira Neto, Manoel P. Mattos, Marise da Silva Pirmez, Claude Sampaio, Elizabeth P. Moraes, Milton O. Cruz, Alda Maria da |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Matos, Guilherme Inocêncio Covas, Claudia de J. Fernandes Bittar, Rita de Cássia Silva, Adriano Gomes Marques, Fabiana Maniero, Viviane C. Amato, Valdir S. Oliveira Neto, Manoel P. Mattos, Marise da Silva Pirmez, Claude Sampaio, Elizabeth P. Moraes, Milton O. Cruz, Alda Maria da |
| dc.subject.other.pt_BR.fl_str_mv |
Leishmania Leishmaniose tegumentar americana Genes reguladores polimórficos |
| topic |
Leishmania Leishmaniose tegumentar americana Genes reguladores polimórficos American tegumentary leishmaniasis Leishmania IFN-γ production IFNG +874T/A polymorphism Polymorphic regulatory genes |
| dc.subject.en.pt_BR.fl_str_mv |
American tegumentary leishmaniasis Leishmania IFN-γ production IFNG +874T/A polymorphism Polymorphic regulatory genes |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Micobacterioses. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. |
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2007 |
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2007 |
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2020-02-13T16:36:49Z |
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2020-02-13T16:36:49Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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MATOS, Guilherme Inocêncio et al. IFNG +874T/A polymorphism is not associated with American tegumentary leishmaniasis susceptibility but can influence Leishmania induced IFN-γ production. BMC Infectious Diseases, v. 7, n. 33, p. 1-6, 2007. |
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https://www.arca.fiocruz.br/handle/icict/39929 |
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1471-2334 |
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10.1186/1471-2334-7-33 |
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MATOS, Guilherme Inocêncio et al. IFNG +874T/A polymorphism is not associated with American tegumentary leishmaniasis susceptibility but can influence Leishmania induced IFN-γ production. BMC Infectious Diseases, v. 7, n. 33, p. 1-6, 2007. 1471-2334 10.1186/1471-2334-7-33 |
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BioMed Central |
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BioMed Central |
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