Antibody Repertoires Identify β-Tubulin as a Host Protective Parasite Antigen in Mice Infected With Trypanosoma cruzi

Montalvão, Fabricio; Nascimento, Danielle Oliveira; Nunes, Marise P.; Koeller, Carolina M.; Morrot, Alexandre; Lery, Leticia Miranda S.; Bisch, Paulo M.; Teixeira, Santuza M. R.; Vasconcellos, Rita; Lima, Leonardo Freire de; Lopes, Marcela F.; Heise, Norton; Reis, George A. dos; Lima, Célio Geraldo Freire de

Antibody Repertoires Identify β-Tubulin as a Host Protective Parasite Antigen in Mice Infected With Trypanosoma cruzi

Detalhes bibliográficos
Autor(a) principal:	Montalvão, Fabricio
Data de Publicação:	2018
Outros Autores:	Nascimento, Danielle Oliveira, Nunes, Marise P., Koeller, Carolina M., Morrot, Alexandre, Lery, Leticia Miranda S., Bisch, Paulo M., Teixeira, Santuza M. R., Vasconcellos, Rita, Lima, Leonardo Freire de, Lopes, Marcela F., Heise, Norton, Reis, George A. dos, Lima, Célio Geraldo Freire de
Tipo de documento:	Artigo
Idioma:	eng
Título da fonte:	Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo:	https://www.arca.fiocruz.br/handle/icict/28465
Resumo:	Faculdade de Medicina de Petrópolis. Petrópolis, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.

Metadados do item

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spelling	Montalvão, FabricioNascimento, Danielle OliveiraNunes, Marise P.Koeller, Carolina M.Morrot, AlexandreLery, Leticia Miranda S.Bisch, Paulo M.Teixeira, Santuza M. R.Vasconcellos, RitaLima, Leonardo Freire deLopes, Marcela F.Heise, NortonReis, George A. dosLima, Célio Geraldo Freire de2018-08-30T13:01:20Z2018-08-30T13:01:20Z2018MONTALVÃO, Fabrício; et al. Antibody Repertoires Identify β-Tubulin as a Host Protective Parasite Antigen in Mice Infected With Trypanosoma cruzi. Frontiers in Immunology, v.9, Article 671, 9p, Apr. 2018.1664-3224https://www.arca.fiocruz.br/handle/icict/2846510.3389/fimmu.2018.00671engFrontiers MediaTrypanosoma cruziDoença de ChagasTubulinaAtivação LinfocitáriaTrypanosoma cruziantibody repertoiresbeta-tubulinlymphocyte activationChagas diseaseAntibody Repertoires Identify β-Tubulin as a Host Protective Parasite Antigen in Mice Infected With Trypanosoma cruziinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFaculdade de Medicina de Petrópolis. Petrópolis, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ. Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ. Brasil / Universidade Federal do Rio de Janeiro. Faculdade de Medicina. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Universidade Federal Fluminense. Instituto de Biologia. Niterói, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Few studies investigate the major protein antigens targeted by the antibody diversity of infected mice with Trypanosoma cruzi. To detect global IgG antibody specificities, sera from infected mice were immunoblotted against whole T. cruzi extracts. By proteomic analysis, we were able to identify the most immunogenic T. cruzi proteins. We identified three major antigens as pyruvate phosphate dikinase, Hsp-85, and β-tubulin. The major protein band recognized by host IgG was T. cruzi β-tubulin. The T. cruzi β-tubulin gene was cloned, expressed in E. coli, and recombinant T. cruzi β-tubulin was obtained. Infection increased IgG reactivity against recombinant T. cruzi β-tubulin. A single immunization of mice with recombinant T. cruzi β-tubulin increased specific IgG reactivity and induced protection against T. cruzi infection. These results indicate that repertoire analysis is a valid approach to identify antigens for vaccines against Chagas disease.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/28465/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALalexandre_morrot_etal_IOC_2018.pdfalexandre_morrot_etal_IOC_2018.pdfapplication/pdf835912https://www.arca.fiocruz.br/bitstream/icict/28465/2/alexandre_morrot_etal_IOC_2018.pdfa495322bc6ca1df32c9da063f5ee3ba8MD52TEXTalexandre_morrot_etal_IOC_2018.pdf.txtalexandre_morrot_etal_IOC_2018.pdf.txtExtracted texttext/plain51423https://www.arca.fiocruz.br/bitstream/icict/28465/3/alexandre_morrot_etal_IOC_2018.pdf.txt4ec380d4c3b82474d35244c3d339e71dMD53icict/284652018-08-31 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dc.title.pt_BR.fl_str_mv	Antibody Repertoires Identify β-Tubulin as a Host Protective Parasite Antigen in Mice Infected With Trypanosoma cruzi
title	Antibody Repertoires Identify β-Tubulin as a Host Protective Parasite Antigen in Mice Infected With Trypanosoma cruzi
spellingShingle	Antibody Repertoires Identify β-Tubulin as a Host Protective Parasite Antigen in Mice Infected With Trypanosoma cruzi Montalvão, Fabricio Trypanosoma cruzi Doença de Chagas Tubulina Ativação Linfocitária Trypanosoma cruzi antibody repertoires beta-tubulin lymphocyte activation Chagas disease
title_short	Antibody Repertoires Identify β-Tubulin as a Host Protective Parasite Antigen in Mice Infected With Trypanosoma cruzi
title_full	Antibody Repertoires Identify β-Tubulin as a Host Protective Parasite Antigen in Mice Infected With Trypanosoma cruzi
title_fullStr	Antibody Repertoires Identify β-Tubulin as a Host Protective Parasite Antigen in Mice Infected With Trypanosoma cruzi
title_full_unstemmed	Antibody Repertoires Identify β-Tubulin as a Host Protective Parasite Antigen in Mice Infected With Trypanosoma cruzi
title_sort	Antibody Repertoires Identify β-Tubulin as a Host Protective Parasite Antigen in Mice Infected With Trypanosoma cruzi
author	Montalvão, Fabricio
author_facet	Montalvão, Fabricio Nascimento, Danielle Oliveira Nunes, Marise P. Koeller, Carolina M. Morrot, Alexandre Lery, Leticia Miranda S. Bisch, Paulo M. Teixeira, Santuza M. R. Vasconcellos, Rita Lima, Leonardo Freire de Lopes, Marcela F. Heise, Norton Reis, George A. dos Lima, Célio Geraldo Freire de
author_role	author
author2	Nascimento, Danielle Oliveira Nunes, Marise P. Koeller, Carolina M. Morrot, Alexandre Lery, Leticia Miranda S. Bisch, Paulo M. Teixeira, Santuza M. R. Vasconcellos, Rita Lima, Leonardo Freire de Lopes, Marcela F. Heise, Norton Reis, George A. dos Lima, Célio Geraldo Freire de
author2_role	author author author author author author author author author author author author author
dc.contributor.author.fl_str_mv	Montalvão, Fabricio Nascimento, Danielle Oliveira Nunes, Marise P. Koeller, Carolina M. Morrot, Alexandre Lery, Leticia Miranda S. Bisch, Paulo M. Teixeira, Santuza M. R. Vasconcellos, Rita Lima, Leonardo Freire de Lopes, Marcela F. Heise, Norton Reis, George A. dos Lima, Célio Geraldo Freire de
dc.subject.other.pt_BR.fl_str_mv	Trypanosoma cruzi Doença de Chagas Tubulina Ativação Linfocitária
topic	Trypanosoma cruzi Doença de Chagas Tubulina Ativação Linfocitária Trypanosoma cruzi antibody repertoires beta-tubulin lymphocyte activation Chagas disease
dc.subject.en.pt_BR.fl_str_mv	Trypanosoma cruzi antibody repertoires beta-tubulin lymphocyte activation Chagas disease
description	Faculdade de Medicina de Petrópolis. Petrópolis, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.
publishDate	2018
dc.date.accessioned.fl_str_mv	2018-08-30T13:01:20Z
dc.date.available.fl_str_mv	2018-08-30T13:01:20Z
dc.date.issued.fl_str_mv	2018
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	MONTALVÃO, Fabrício; et al. Antibody Repertoires Identify β-Tubulin as a Host Protective Parasite Antigen in Mice Infected With Trypanosoma cruzi. Frontiers in Immunology, v.9, Article 671, 9p, Apr. 2018.
dc.identifier.uri.fl_str_mv	https://www.arca.fiocruz.br/handle/icict/28465
dc.identifier.issn.pt_BR.fl_str_mv	1664-3224
dc.identifier.doi.none.fl_str_mv	10.3389/fimmu.2018.00671
identifier_str_mv	MONTALVÃO, Fabrício; et al. Antibody Repertoires Identify β-Tubulin as a Host Protective Parasite Antigen in Mice Infected With Trypanosoma cruzi. Frontiers in Immunology, v.9, Article 671, 9p, Apr. 2018. 1664-3224 10.3389/fimmu.2018.00671
url	https://www.arca.fiocruz.br/handle/icict/28465
dc.language.iso.fl_str_mv	eng
language	eng
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.publisher.none.fl_str_mv	Frontiers Media
publisher.none.fl_str_mv	Frontiers Media
dc.source.none.fl_str_mv	reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ
instname_str	Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str	FIOCRUZ
institution	FIOCRUZ
reponame_str	Repositório Institucional da FIOCRUZ (ARCA)
collection	Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv	https://www.arca.fiocruz.br/bitstream/icict/28465/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/28465/2/alexandre_morrot_etal_IOC_2018.pdf https://www.arca.fiocruz.br/bitstream/icict/28465/3/alexandre_morrot_etal_IOC_2018.pdf.txt
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repository.name.fl_str_mv	Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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Antibody Repertoires Identify β-Tubulin as a Host Protective Parasite Antigen in Mice Infected With Trypanosoma cruzi

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