Antigenic extracts of Leishmania braziliensis and Leishmania amazonensis associated with saponin partially protects BALB/c mice against Leishmania chagasi infection by suppressing IL-10 and IL-4 production

Detalhes bibliográficos
Autor(a) principal: Queiroz, Rafaella Fortini Grenfell
Data de Publicação: 2010
Outros Autores: Silva, Eduardo de Almeida Marques da, Testasicca, Míriam conceição de Souza, Coelho, Eduardo Antonio Ferraz, Fernandes, Ana Paula, Afonso, Luiz Carlos Crocco, Rezende, Simone Aparecida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/27688
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil / Instituto Federal de Minas Gerais. Coordenadoria de Ciências Biológicas. Laboratório de Imunoparasitologia. Belo Horizonte, MG, Brasil.
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spelling Queiroz, Rafaella Fortini GrenfellSilva, Eduardo de Almeida Marques daTestasicca, Míriam conceição de SouzaCoelho, Eduardo Antonio FerrazFernandes, Ana PaulaAfonso, Luiz Carlos CroccoRezende, Simone Aparecida2018-07-27T17:06:28Z2018-07-27T17:06:28Z2010QUEIROZ , Rafaella Fortini Grenfell et al. Antigenic extracts of Leishmania braziliensis and Leishmania amazonensis associated with saponin partially protects BALB/c mice against Leishmania chagasi infection by suppressing IL-10 and IL-4 production. Mem. Inst. Oswaldo Cruz, Rio de Janeiro, v. 105, n. 6, p. 818-822, Sept. 2010.0074-0276https://www.arca.fiocruz.br/handle/icict/2768810.1590/S0074-02762010000600015engFundação Oswaldo CruzVacinaLeishmanioseVaccineLeishmania chagasiLeishmania braziliensisLeishmania amazonensisIL-10 - IL-4Antigenic extracts of Leishmania braziliensis and Leishmania amazonensis associated with saponin partially protects BALB/c mice against Leishmania chagasi infection by suppressing IL-10 and IL-4 productioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil / Instituto Federal de Minas Gerais. Coordenadoria de Ciências Biológicas. Laboratório de Imunoparasitologia. Belo Horizonte, MG, Brasil.Instituto Federal de Minas Gerais. Coordenadoria de Ciências Biológicas. Laboratório de Imunoparasitologia. Belo Horizonte, MG, Brasil.Instituto Federal de Minas Gerais. Coordenadoria de Ciências Biológicas. Laboratório de Imunoparasitologia. Belo Horizonte, MG, Brasil.Instituto Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil / Colégio Técnico. Setor de Patologia Clínica. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Microbiologia. Belo Horizonte, MG, Brasil.Instituto Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Instituto Federal de Minas Gerais. Coordenadoria de Ciências Biológicas. Laboratório de Imunoparasitologia. Belo Horizonte, MG, Brasil.Instituto Federal de Minas Gerais. Coordenadoria de Ciências Biológicas. Laboratório de Imunoparasitologia. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clínicas. Belo Horizonte, MG, Brasil.This study evaluated two vaccine candidates for their effectiveness in protecting BALB/c mice against Leishmania chagasiinfection. These immunogenic preparations were composed of Leishmania amazonensisor Leishmania braziliensisantigenic extracts in association with saponin adjuvant. Mice were given three subcutaneous doses of one of these vaccine candidates weekly for three weeks and four weeks later challenged with promastigotes of L. chagasiby intravenous injection. We observed that both vaccine candidates induced a significant reduction in the parasite load of the liver, while the L. amazonensisantigenic extract also stimulated a reduction in spleen parasite load. This protection was associated with a suppression of both interleukin (IL)-10 and IL-4 cytokines by spleen cells in response to L. chagasiantigen. No change was detected in the production of IFN-γ. Our data show that these immunogenic preparations reduce the type 2 immune response leading to the control of parasite replication.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/27688/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALAntigenic extracts of Leishmania.pdfAntigenic extracts of Leishmania.pdfapplication/pdf440875https://www.arca.fiocruz.br/bitstream/icict/27688/2/Antigenic%20extracts%20of%20Leishmania.pdfebb69568857b1bb9a5b18a2542d8ab6eMD52TEXTAntigenic extracts of Leishmania.pdf.txtAntigenic extracts of Leishmania.pdf.txtExtracted texttext/plain31019https://www.arca.fiocruz.br/bitstream/icict/27688/3/Antigenic%20extracts%20of%20Leishmania.pdf.txt9824e27e1969c7fdf9aa011c2995549aMD53icict/276882019-06-19 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dc.title.pt_BR.fl_str_mv Antigenic extracts of Leishmania braziliensis and Leishmania amazonensis associated with saponin partially protects BALB/c mice against Leishmania chagasi infection by suppressing IL-10 and IL-4 production
title Antigenic extracts of Leishmania braziliensis and Leishmania amazonensis associated with saponin partially protects BALB/c mice against Leishmania chagasi infection by suppressing IL-10 and IL-4 production
spellingShingle Antigenic extracts of Leishmania braziliensis and Leishmania amazonensis associated with saponin partially protects BALB/c mice against Leishmania chagasi infection by suppressing IL-10 and IL-4 production
Queiroz, Rafaella Fortini Grenfell
Vacina
Leishmaniose
Vaccine
Leishmania chagasi
Leishmania braziliensis
Leishmania amazonensis
IL-10 - IL-4
title_short Antigenic extracts of Leishmania braziliensis and Leishmania amazonensis associated with saponin partially protects BALB/c mice against Leishmania chagasi infection by suppressing IL-10 and IL-4 production
title_full Antigenic extracts of Leishmania braziliensis and Leishmania amazonensis associated with saponin partially protects BALB/c mice against Leishmania chagasi infection by suppressing IL-10 and IL-4 production
title_fullStr Antigenic extracts of Leishmania braziliensis and Leishmania amazonensis associated with saponin partially protects BALB/c mice against Leishmania chagasi infection by suppressing IL-10 and IL-4 production
title_full_unstemmed Antigenic extracts of Leishmania braziliensis and Leishmania amazonensis associated with saponin partially protects BALB/c mice against Leishmania chagasi infection by suppressing IL-10 and IL-4 production
title_sort Antigenic extracts of Leishmania braziliensis and Leishmania amazonensis associated with saponin partially protects BALB/c mice against Leishmania chagasi infection by suppressing IL-10 and IL-4 production
author Queiroz, Rafaella Fortini Grenfell
author_facet Queiroz, Rafaella Fortini Grenfell
Silva, Eduardo de Almeida Marques da
Testasicca, Míriam conceição de Souza
Coelho, Eduardo Antonio Ferraz
Fernandes, Ana Paula
Afonso, Luiz Carlos Crocco
Rezende, Simone Aparecida
author_role author
author2 Silva, Eduardo de Almeida Marques da
Testasicca, Míriam conceição de Souza
Coelho, Eduardo Antonio Ferraz
Fernandes, Ana Paula
Afonso, Luiz Carlos Crocco
Rezende, Simone Aparecida
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Queiroz, Rafaella Fortini Grenfell
Silva, Eduardo de Almeida Marques da
Testasicca, Míriam conceição de Souza
Coelho, Eduardo Antonio Ferraz
Fernandes, Ana Paula
Afonso, Luiz Carlos Crocco
Rezende, Simone Aparecida
dc.subject.other.pt_BR.fl_str_mv Vacina
Leishmaniose
topic Vacina
Leishmaniose
Vaccine
Leishmania chagasi
Leishmania braziliensis
Leishmania amazonensis
IL-10 - IL-4
dc.subject.en.pt_BR.fl_str_mv Vaccine
Leishmania chagasi
Leishmania braziliensis
Leishmania amazonensis
IL-10 - IL-4
description Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil / Instituto Federal de Minas Gerais. Coordenadoria de Ciências Biológicas. Laboratório de Imunoparasitologia. Belo Horizonte, MG, Brasil.
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2018-07-27T17:06:28Z
dc.date.available.fl_str_mv 2018-07-27T17:06:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv QUEIROZ , Rafaella Fortini Grenfell et al. Antigenic extracts of Leishmania braziliensis and Leishmania amazonensis associated with saponin partially protects BALB/c mice against Leishmania chagasi infection by suppressing IL-10 and IL-4 production. Mem. Inst. Oswaldo Cruz, Rio de Janeiro, v. 105, n. 6, p. 818-822, Sept. 2010.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/27688
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762010000600015
identifier_str_mv QUEIROZ , Rafaella Fortini Grenfell et al. Antigenic extracts of Leishmania braziliensis and Leishmania amazonensis associated with saponin partially protects BALB/c mice against Leishmania chagasi infection by suppressing IL-10 and IL-4 production. Mem. Inst. Oswaldo Cruz, Rio de Janeiro, v. 105, n. 6, p. 818-822, Sept. 2010.
0074-0276
10.1590/S0074-02762010000600015
url https://www.arca.fiocruz.br/handle/icict/27688
dc.language.iso.fl_str_mv eng
language eng
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eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
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