Impact of reduced levels of APE1 transcripts on the survival of patients with urothelial carcinoma of the bladder
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/13543 |
Resumo: | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Rio de Janeiro. Departamento de Patologia. Laboratório de Marcadores Circulantes. Rio de Janeiro, RJ, Brasil. |
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Justino, Mariana ChantreAlves, GildaBritto, ConstançaCardoso, AngélicaScherrer, LucianoMoreira, Aline dos SantosQuirino, RaulOrnellas, AntonioLeitão, AlvaroLage, Claudia2016-04-05T17:38:32Z2016-04-05T17:38:32Z2015JUSTINO, Mariana Chantre; et al. Impact of reduced levels of APE1 transcripts on the survival of patients with urothelial carcinoma of the bladder. Oncology Reports, v.34, n.4, p.1667-1674, Oct. 2015.1021-335Xhttps://www.arca.fiocruz.br/handle/icict/1354310.3892/or.2015.41511791-2431engSpandidos PublicationsImpact of reduced levels of APE1 transcripts on the survival of patients with urothelial carcinoma of the bladderinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Rio de Janeiro. Departamento de Patologia. Laboratório de Marcadores Circulantes. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Câncer. Coordenação de Pesquisa. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Rio de Janeiro. Departamento de Patologia. Laboratório de Marcadores Circulantes. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Sociedade Brasileira de Oncologia Clínica. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. PDTIS Plataforma de Sequenciamento de DNA. Brasil.Instituto Nacional de Câncer. Departamento de Urologia. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Câncer. Departamento de Urologia. Rio de Janeiro, RJ, Brasil / Hospital Mário Kröeff. Departamento de Urologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Abstract Molecular evidence indicates that alterations in genes involved in the maintenance of genome stability may be related to susceptibility to bladder carcinoma. Our goal was to evaluate the prognostic role of base excision repair (BER) genes in a cohort of patients diagnosed with primary urothelial carcinoma of the bladder (UCB). The levels of all APE1, XRCC1 and POLB transcripts were detected by quantitative real-time PCR (qPCR) technique in tumor samples from 52 patients undergoing transurethral resection (TUR) for primary UCB at the Department of Urology, Brazilian National Cancer Institute, Rio de Janeiro. Increased levels of APE1, XRCC1 and POLB transcripts were significantly associated with high-grade tumors when compared to these levels in low-grade tumors (p<0.01) and could be attributed to different mechanisms of transcriptional regulation as a response to tumorigenesis and oxidative stress. By analyzing the collected data in the present study, regardless of pathological grade or stage, univariate analysis revealed that the reduced levels of APE1 transcripts were significantly associated with cancer-specific mortality (p=0.032). Furthermore, the variant genotype (TG/GG) of the APE1 T1349G polymorphism was observed in 75% of a subset of patients who concomitantly experienced reduced levels of the APE1 transcript and death and/or recurrence events. Taken together, our data reinforce the idea that human DNA repair mechanisms must be finely regulated in order to avoid instability leading to tumorigenesis and poor clinical outcomes in UCB patients.Urothelial carcinomaBladderBexiga UrináriaCâncerinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/13543/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALangelica_cardoso_etal_IOC_2015.pdfangelica_cardoso_etal_IOC_2015.pdfapplication/pdf669376https://www.arca.fiocruz.br/bitstream/icict/13543/2/angelica_cardoso_etal_IOC_2015.pdfbd50c616ae2a51797812897231893d1dMD52TEXTangelica_cardoso_etal_IOC_2015.pdf.txtangelica_cardoso_etal_IOC_2015.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv |
Impact of reduced levels of APE1 transcripts on the survival of patients with urothelial carcinoma of the bladder |
title |
Impact of reduced levels of APE1 transcripts on the survival of patients with urothelial carcinoma of the bladder |
spellingShingle |
Impact of reduced levels of APE1 transcripts on the survival of patients with urothelial carcinoma of the bladder Justino, Mariana Chantre Urothelial carcinoma Bladder Bexiga Urinária Câncer |
title_short |
Impact of reduced levels of APE1 transcripts on the survival of patients with urothelial carcinoma of the bladder |
title_full |
Impact of reduced levels of APE1 transcripts on the survival of patients with urothelial carcinoma of the bladder |
title_fullStr |
Impact of reduced levels of APE1 transcripts on the survival of patients with urothelial carcinoma of the bladder |
title_full_unstemmed |
Impact of reduced levels of APE1 transcripts on the survival of patients with urothelial carcinoma of the bladder |
title_sort |
Impact of reduced levels of APE1 transcripts on the survival of patients with urothelial carcinoma of the bladder |
author |
Justino, Mariana Chantre |
author_facet |
Justino, Mariana Chantre Alves, Gilda Britto, Constança Cardoso, Angélica Scherrer, Luciano Moreira, Aline dos Santos Quirino, Raul Ornellas, Antonio Leitão, Alvaro Lage, Claudia |
author_role |
author |
author2 |
Alves, Gilda Britto, Constança Cardoso, Angélica Scherrer, Luciano Moreira, Aline dos Santos Quirino, Raul Ornellas, Antonio Leitão, Alvaro Lage, Claudia |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Justino, Mariana Chantre Alves, Gilda Britto, Constança Cardoso, Angélica Scherrer, Luciano Moreira, Aline dos Santos Quirino, Raul Ornellas, Antonio Leitão, Alvaro Lage, Claudia |
dc.subject.en.pt_BR.fl_str_mv |
Urothelial carcinoma Bladder |
topic |
Urothelial carcinoma Bladder Bexiga Urinária Câncer |
dc.subject.decs.pt_BR.fl_str_mv |
Bexiga Urinária Câncer |
description |
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Rio de Janeiro. Departamento de Patologia. Laboratório de Marcadores Circulantes. Rio de Janeiro, RJ, Brasil. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015 |
dc.date.accessioned.fl_str_mv |
2016-04-05T17:38:32Z |
dc.date.available.fl_str_mv |
2016-04-05T17:38:32Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
JUSTINO, Mariana Chantre; et al. Impact of reduced levels of APE1 transcripts on the survival of patients with urothelial carcinoma of the bladder. Oncology Reports, v.34, n.4, p.1667-1674, Oct. 2015. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/13543 |
dc.identifier.issn.none.fl_str_mv |
1021-335X |
dc.identifier.doi.none.fl_str_mv |
10.3892/or.2015.4151 |
dc.identifier.eissn.none.fl_str_mv |
1791-2431 |
identifier_str_mv |
JUSTINO, Mariana Chantre; et al. Impact of reduced levels of APE1 transcripts on the survival of patients with urothelial carcinoma of the bladder. Oncology Reports, v.34, n.4, p.1667-1674, Oct. 2015. 1021-335X 10.3892/or.2015.4151 1791-2431 |
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https://www.arca.fiocruz.br/handle/icict/13543 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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Spandidos Publications |
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Spandidos Publications |
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