Characterization of the Temporal Pattern of Blood Protein Digestion in Rhodnius prolixus: First Description of Early and Late Gut Cathepsins
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/48147 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil. |
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Henriques, Bianca SantosGomes, BrunoOliveira, Pedro LagerbladGarcia, Eloi de SouzaAzambuja, PatriciaGenta, Fernando Ariel2021-07-12T14:01:51Z2021-07-12T14:01:51Z2021HENRIQUES, Bianca Santos et al. Characterization of the Temporal Pattern of Blood Protein Digestion in Rhodnius prolixus: First Description of Early and Late Gut Cathepsins. Front. Physiol., v. 11, Article 509310, 20 p, Jan. 2021.1664-042Xhttps://www.arca.fiocruz.br/handle/icict/4814710.3389/fphys.2020.509310engFrontiers MediaRhodnius prolixusProteaseDigestão proteicaCathepsin L.Cathepsin DRhodnius prolixusProteaseProtein digestionCathepsin L.Cathepsin DCharacterization of the Temporal Pattern of Blood Protein Digestion in Rhodnius prolixus: First Description of Early and Late Gut Cathepsinsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Ciência e Tecnologia para Entomologia Molecular. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para Entomologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para Entomologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para Entomologia Molecular. Rio de Janeiro, RJ, Brasil.Rhodnius prolixus is one important vector for the parasite Trypanosoma cruzi in Latin America, where Chagas disease is a significant health issue. Although R. prolixus is a model for investigations of vector–parasite interaction and transmission, not much has been done recently to further comprehend its protein digestion. In this work, gut proteolysis was characterized using new fluorogenic substrates, including optimum pH, inhibition profiles, and tissue and temporal expression patterns. Each protease possessed a particular tissue prevalence and activity cycle after feeding. Cathepsin L had a higher activity in the posterior midgut lumen, being characterized by a plateau of high activities during several days in the intermediate phase of digestion. Cathepsin D showed high activity levels in the tissue homogenates and in the luminal content of the posterior midgut, with a single peak 5 days after blood feeding. Aminopeptidases are highly associated with the midgut wall, where the highest activity is located. Assays with proteinaceous substrates as casein, hemoglobin, and serum albumin revealed different activity profiles, with some evidence of biphasic temporal proteolytic patterns. Cathepsin D genes are preferentially expressed in the anterior midgut, while cathepsin L genes are mainly located in the posterior portion of the midgut, with specific sets of genes being differently expressed in the initial, intermediate, or late phases of blood digestion.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/48147/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALBiancaHenriques_EloiGarcia_etal_IOC_2021.pdfBiancaHenriques_EloiGarcia_etal_IOC_2021.pdfapplication/pdf4296765https://www.arca.fiocruz.br/bitstream/icict/48147/2/BiancaHenriques_EloiGarcia_etal_IOC_2021.pdfe276af12c742e55ded4ec0625509d0a2MD52TEXTBiancaHenriques_EloiGarcia_etal_IOC_2021.pdf.txtBiancaHenriques_EloiGarcia_etal_IOC_2021.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Characterization of the Temporal Pattern of Blood Protein Digestion in Rhodnius prolixus: First Description of Early and Late Gut Cathepsins |
| title |
Characterization of the Temporal Pattern of Blood Protein Digestion in Rhodnius prolixus: First Description of Early and Late Gut Cathepsins |
| spellingShingle |
Characterization of the Temporal Pattern of Blood Protein Digestion in Rhodnius prolixus: First Description of Early and Late Gut Cathepsins Henriques, Bianca Santos Rhodnius prolixus Protease Digestão proteica Cathepsin L. Cathepsin D Rhodnius prolixus Protease Protein digestion Cathepsin L. Cathepsin D |
| title_short |
Characterization of the Temporal Pattern of Blood Protein Digestion in Rhodnius prolixus: First Description of Early and Late Gut Cathepsins |
| title_full |
Characterization of the Temporal Pattern of Blood Protein Digestion in Rhodnius prolixus: First Description of Early and Late Gut Cathepsins |
| title_fullStr |
Characterization of the Temporal Pattern of Blood Protein Digestion in Rhodnius prolixus: First Description of Early and Late Gut Cathepsins |
| title_full_unstemmed |
Characterization of the Temporal Pattern of Blood Protein Digestion in Rhodnius prolixus: First Description of Early and Late Gut Cathepsins |
| title_sort |
Characterization of the Temporal Pattern of Blood Protein Digestion in Rhodnius prolixus: First Description of Early and Late Gut Cathepsins |
| author |
Henriques, Bianca Santos |
| author_facet |
Henriques, Bianca Santos Gomes, Bruno Oliveira, Pedro Lagerblad Garcia, Eloi de Souza Azambuja, Patricia Genta, Fernando Ariel |
| author_role |
author |
| author2 |
Gomes, Bruno Oliveira, Pedro Lagerblad Garcia, Eloi de Souza Azambuja, Patricia Genta, Fernando Ariel |
| author2_role |
author author author author author |
| dc.contributor.author.fl_str_mv |
Henriques, Bianca Santos Gomes, Bruno Oliveira, Pedro Lagerblad Garcia, Eloi de Souza Azambuja, Patricia Genta, Fernando Ariel |
| dc.subject.other.pt_BR.fl_str_mv |
Rhodnius prolixus Protease Digestão proteica Cathepsin L. Cathepsin D |
| topic |
Rhodnius prolixus Protease Digestão proteica Cathepsin L. Cathepsin D Rhodnius prolixus Protease Protein digestion Cathepsin L. Cathepsin D |
| dc.subject.en.pt_BR.fl_str_mv |
Rhodnius prolixus Protease Protein digestion Cathepsin L. Cathepsin D |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil. |
| publishDate |
2021 |
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2021-07-12T14:01:51Z |
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2021-07-12T14:01:51Z |
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2021 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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HENRIQUES, Bianca Santos et al. Characterization of the Temporal Pattern of Blood Protein Digestion in Rhodnius prolixus: First Description of Early and Late Gut Cathepsins. Front. Physiol., v. 11, Article 509310, 20 p, Jan. 2021. |
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https://www.arca.fiocruz.br/handle/icict/48147 |
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1664-042X |
| dc.identifier.doi.none.fl_str_mv |
10.3389/fphys.2020.509310 |
| identifier_str_mv |
HENRIQUES, Bianca Santos et al. Characterization of the Temporal Pattern of Blood Protein Digestion in Rhodnius prolixus: First Description of Early and Late Gut Cathepsins. Front. Physiol., v. 11, Article 509310, 20 p, Jan. 2021. 1664-042X 10.3389/fphys.2020.509310 |
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