Effect of 3-Bromopyruvate and Atovaquone on Infection during In Vitro Interaction of Toxoplasma gondii and LLC-MK2 Cells

Detalhes bibliográficos
Autor(a) principal: Lima, Loyze Paola O. de
Data de Publicação: 2015
Outros Autores: Seabra, Sergio H., Carneiro, Henrique, Barbosa, Helene S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/13228
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil / Centro Universitário Estadual da Zona Oeste (UEZO). Laboratório de Tecnologia em Bioquímica e Microscopia. Campo Grande, RJ, Brasil.
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spelling Lima, Loyze Paola O. deSeabra, Sergio H.Carneiro, HenriqueBarbosa, Helene S.2016-03-22T12:33:56Z2016-03-22T12:33:56Z2015LIMA, Loyze Paola de O. et al. Effect of 3-Bromopyruvate and Atovaquone on Infection during In Vitro Interaction of Toxoplasma gondii and LLC-MK2 Cells. Antimicrobial Agents and Chemotherapy, v.59, n.9, p.5239-5249, Sept. 2015.1098-6596https://www.arca.fiocruz.br/handle/icict/1322810.3389/fimmu.2015.00615engAmerican Society for MicrobiologyToxoplasmaAtovaquonaMedicamentosTécnicas In Vitro3-BromopyruvateToxoplasma gondii3-BromopyruvateAtovaquoneLLC-MK2 CellsIn Vitro InteractionEffect of 3-Bromopyruvate and Atovaquone on Infection during In Vitro Interaction of Toxoplasma gondii and LLC-MK2 Cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil / Centro Universitário Estadual da Zona Oeste (UEZO). Laboratório de Tecnologia em Bioquímica e Microscopia. Campo Grande, RJ, Brasil.Centro Universitário Estadual da Zona Oeste (UEZO). Laboratório de Tecnologia em Bioquímica e Microscopia. Campo Grande, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil.Toxoplasma gondii infection can be severe during pregnancy and in immunocompromised patients. Current therapies for toxoplasmosis are restricted to tachyzoites and have little or no effect on bradyzoites, which are maintained in tissue cysts. Consequently, new therapeutic alternatives have been proposed as the use of atovaquone has demonstrated partial efficacy against tachyzoites and bradyzoites. This work studies the effect of 3-bromopyruvate (3-BrPA), a compound that is being tested against cancer cells, on the infection of LLC-MK2 cells with T. gondii tachyzoites, RH strain. No effect of 3-BrPA on host cell proliferation or viability was observed, but it inhibited the proliferation of T. gondii. The incubation of cultures with lectin Dolichos bi- florus agglutinin (DBA) showed the development of cystogenesis, and an ultrastructural analysis of parasite intracellular development confirmed morphological characteristics commonly found in tissue cysts. Moreover, the presence of degraded parasites and the influence of 3-BrPA on endodyogeny were observed. Infected cultures were alternatively treated with a combination of this compound plus atovaquone. This resulted in a 73% reduction in intracellular parasites after 24 h of treatment and a 71% reduction after 48 h; cyst wall formation did not occur in these cultures. Therefore, we conclude that the use of 3-BrPA may serve as an important tool for the study of (i) in vitro cystogenesis; (ii) parasite metabolism, requiring a deeper understanding of the target of action of this compound on T. gondii; (iii) the alternative parasite metabolic pathways; and (iv) the molecular/cellular mechanisms that trigger parasite death.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/13228/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALloyze_lima_etal_IOC_2015.pdfloyze_lima_etal_IOC_2015.pdfapplication/pdf4789416https://www.arca.fiocruz.br/bitstream/icict/13228/2/loyze_lima_etal_IOC_2015.pdf8c50fe3ba44eb2a220f078216851c915MD52TEXTloyze_lima_etal_IOC_2015.pdf.txtloyze_lima_etal_IOC_2015.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Effect of 3-Bromopyruvate and Atovaquone on Infection during In Vitro Interaction of Toxoplasma gondii and LLC-MK2 Cells
title Effect of 3-Bromopyruvate and Atovaquone on Infection during In Vitro Interaction of Toxoplasma gondii and LLC-MK2 Cells
spellingShingle Effect of 3-Bromopyruvate and Atovaquone on Infection during In Vitro Interaction of Toxoplasma gondii and LLC-MK2 Cells
Lima, Loyze Paola O. de
Toxoplasma
Atovaquona
Medicamentos
Técnicas In Vitro
3-Bromopyruvate
Toxoplasma gondii
3-Bromopyruvate
Atovaquone
LLC-MK2 Cells
In Vitro Interaction
title_short Effect of 3-Bromopyruvate and Atovaquone on Infection during In Vitro Interaction of Toxoplasma gondii and LLC-MK2 Cells
title_full Effect of 3-Bromopyruvate and Atovaquone on Infection during In Vitro Interaction of Toxoplasma gondii and LLC-MK2 Cells
title_fullStr Effect of 3-Bromopyruvate and Atovaquone on Infection during In Vitro Interaction of Toxoplasma gondii and LLC-MK2 Cells
title_full_unstemmed Effect of 3-Bromopyruvate and Atovaquone on Infection during In Vitro Interaction of Toxoplasma gondii and LLC-MK2 Cells
title_sort Effect of 3-Bromopyruvate and Atovaquone on Infection during In Vitro Interaction of Toxoplasma gondii and LLC-MK2 Cells
author Lima, Loyze Paola O. de
author_facet Lima, Loyze Paola O. de
Seabra, Sergio H.
Carneiro, Henrique
Barbosa, Helene S.
author_role author
author2 Seabra, Sergio H.
Carneiro, Henrique
Barbosa, Helene S.
author2_role author
author
author
dc.contributor.author.fl_str_mv Lima, Loyze Paola O. de
Seabra, Sergio H.
Carneiro, Henrique
Barbosa, Helene S.
dc.subject.other.pt_BR.fl_str_mv Toxoplasma
Atovaquona
Medicamentos
Técnicas In Vitro
3-Bromopyruvate
topic Toxoplasma
Atovaquona
Medicamentos
Técnicas In Vitro
3-Bromopyruvate
Toxoplasma gondii
3-Bromopyruvate
Atovaquone
LLC-MK2 Cells
In Vitro Interaction
dc.subject.en.pt_BR.fl_str_mv Toxoplasma gondii
3-Bromopyruvate
Atovaquone
LLC-MK2 Cells
In Vitro Interaction
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil / Centro Universitário Estadual da Zona Oeste (UEZO). Laboratório de Tecnologia em Bioquímica e Microscopia. Campo Grande, RJ, Brasil.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-03-22T12:33:56Z
dc.date.available.fl_str_mv 2016-03-22T12:33:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv LIMA, Loyze Paola de O. et al. Effect of 3-Bromopyruvate and Atovaquone on Infection during In Vitro Interaction of Toxoplasma gondii and LLC-MK2 Cells. Antimicrobial Agents and Chemotherapy, v.59, n.9, p.5239-5249, Sept. 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/13228
dc.identifier.issn.none.fl_str_mv 1098-6596
dc.identifier.doi.none.fl_str_mv 10.3389/fimmu.2015.00615
identifier_str_mv LIMA, Loyze Paola de O. et al. Effect of 3-Bromopyruvate and Atovaquone on Infection during In Vitro Interaction of Toxoplasma gondii and LLC-MK2 Cells. Antimicrobial Agents and Chemotherapy, v.59, n.9, p.5239-5249, Sept. 2015.
1098-6596
10.3389/fimmu.2015.00615
url https://www.arca.fiocruz.br/handle/icict/13228
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/13228/2/loyze_lima_etal_IOC_2015.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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