Flow Cytometry Evaluation of the T-Cell Receptor Vβ Repertoire Among HIV-1 Infected Individuals Before and After Antiretroviral Therapy

Detalhes bibliográficos
Autor(a) principal: Giacoia-Gripp, Carmem Beatriz Wagner
Data de Publicação: 2005
Outros Autores: Neves Jr., Ivan, Galhardo, Maria Clara, Morgado, Mariza Gonçalves
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/37884
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.
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spelling Giacoia-Gripp, Carmem Beatriz WagnerNeves Jr., IvanGalhardo, Maria ClaraMorgado, Mariza Gonçalves2019-12-12T11:09:45Z2019-12-12T11:09:45Z2005GIACOIA-GRIPP, Carmem Beatriz Wagner et al. Flow Cytometry Evaluation of the T-Cell Receptor Vβ Repertoire Among HIV-1 Infected Individuals Before and After Antiretroviral Therapy. Journal of Clinical Immunology, v. 25, n. 2, p. 116-126, Mar. 2005.0271-9142https://www.arca.fiocruz.br/handle/icict/3788410.1007/s10875-005-2817-z1573-2592engSpringerHIV-1ClonalidadeRepertório TCR vβHIV-1TCR Vβ repertoireHAARTClonalityFlow Cytometry Evaluation of the T-Cell Receptor Vβ Repertoire Among HIV-1 Infected Individuals Before and After Antiretroviral Therapyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Departamento de Micro-Imuno-Parasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Departamento de Doenças Infecciosas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.HIV-1 infection leads to serious impairment of the immune system and perturbations in the T cell receptorVβ repertoire are also described. Immune reconstitution can be potentially achieved in response to HAART. In the present study 10 patients were investigated for the Vβ pattern expression before and after six months of HAART. TCR were analyzed for T CD4+ and CD8+ subsets, separately, by flow cytometry, using a monoclonal antibody set of 24 different Vβ chains. Compared to eight Brazilian healthy controls, no differences in Vβ pattern of expression was observed for patients before or on antiretroviral therapy. Some chains such as Vβ 3, 14, 16, 20 and 21.3 were over utilized by both T subsets, independently of HIV infection and/or antiretroviral treatment, differing from the ones described for individuals of other nationalities. However, when each patient was taken individually, particular alterations were detected for the Vβ gene usage, compared to controls, for all individuals. After treatment, significantVβ usage changes were observed for seven patients. One or more chains on both T subsets were engaged in this process, defining a preferential oligoclonal profile for TCR repertoire distribution, after HAART. Although no pattern of specific Vβ changes was detected in the circulating T cells, we cannot exclude that differential immune responses to HIV or other important antigens are being focused by these cells.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/37884/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMarizaG_Morgado_etal_IOC_2005.pdfMarizaG_Morgado_etal_IOC_2005.pdfapplication/pdf156927https://www.arca.fiocruz.br/bitstream/icict/37884/2/MarizaG_Morgado_etal_IOC_2005.pdf768faed9fa4a8f3bb039ed71b2bcfc26MD52TEXTMarizaG_Morgado_etal_IOC_2005.pdf.txtMarizaG_Morgado_etal_IOC_2005.pdf.txtExtracted texttext/plain45683https://www.arca.fiocruz.br/bitstream/icict/37884/3/MarizaG_Morgado_etal_IOC_2005.pdf.txtac4a465f0a002d78fb6103db535f310fMD53icict/378842019-12-13 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dc.title.pt_BR.fl_str_mv Flow Cytometry Evaluation of the T-Cell Receptor Vβ Repertoire Among HIV-1 Infected Individuals Before and After Antiretroviral Therapy
title Flow Cytometry Evaluation of the T-Cell Receptor Vβ Repertoire Among HIV-1 Infected Individuals Before and After Antiretroviral Therapy
spellingShingle Flow Cytometry Evaluation of the T-Cell Receptor Vβ Repertoire Among HIV-1 Infected Individuals Before and After Antiretroviral Therapy
Giacoia-Gripp, Carmem Beatriz Wagner
HIV-1
Clonalidade
Repertório TCR vβ
HIV-1
TCR Vβ repertoire
HAART
Clonality
title_short Flow Cytometry Evaluation of the T-Cell Receptor Vβ Repertoire Among HIV-1 Infected Individuals Before and After Antiretroviral Therapy
title_full Flow Cytometry Evaluation of the T-Cell Receptor Vβ Repertoire Among HIV-1 Infected Individuals Before and After Antiretroviral Therapy
title_fullStr Flow Cytometry Evaluation of the T-Cell Receptor Vβ Repertoire Among HIV-1 Infected Individuals Before and After Antiretroviral Therapy
title_full_unstemmed Flow Cytometry Evaluation of the T-Cell Receptor Vβ Repertoire Among HIV-1 Infected Individuals Before and After Antiretroviral Therapy
title_sort Flow Cytometry Evaluation of the T-Cell Receptor Vβ Repertoire Among HIV-1 Infected Individuals Before and After Antiretroviral Therapy
author Giacoia-Gripp, Carmem Beatriz Wagner
author_facet Giacoia-Gripp, Carmem Beatriz Wagner
Neves Jr., Ivan
Galhardo, Maria Clara
Morgado, Mariza Gonçalves
author_role author
author2 Neves Jr., Ivan
Galhardo, Maria Clara
Morgado, Mariza Gonçalves
author2_role author
author
author
dc.contributor.author.fl_str_mv Giacoia-Gripp, Carmem Beatriz Wagner
Neves Jr., Ivan
Galhardo, Maria Clara
Morgado, Mariza Gonçalves
dc.subject.other.pt_BR.fl_str_mv HIV-1
Clonalidade
Repertório TCR vβ
topic HIV-1
Clonalidade
Repertório TCR vβ
HIV-1
TCR Vβ repertoire
HAART
Clonality
dc.subject.en.pt_BR.fl_str_mv HIV-1
TCR Vβ repertoire
HAART
Clonality
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.
publishDate 2005
dc.date.issued.fl_str_mv 2005
dc.date.accessioned.fl_str_mv 2019-12-12T11:09:45Z
dc.date.available.fl_str_mv 2019-12-12T11:09:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GIACOIA-GRIPP, Carmem Beatriz Wagner et al. Flow Cytometry Evaluation of the T-Cell Receptor Vβ Repertoire Among HIV-1 Infected Individuals Before and After Antiretroviral Therapy. Journal of Clinical Immunology, v. 25, n. 2, p. 116-126, Mar. 2005.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/37884
dc.identifier.issn.pt_BR.fl_str_mv 0271-9142
dc.identifier.doi.none.fl_str_mv 10.1007/s10875-005-2817-z
dc.identifier.eissn.none.fl_str_mv 1573-2592
identifier_str_mv GIACOIA-GRIPP, Carmem Beatriz Wagner et al. Flow Cytometry Evaluation of the T-Cell Receptor Vβ Repertoire Among HIV-1 Infected Individuals Before and After Antiretroviral Therapy. Journal of Clinical Immunology, v. 25, n. 2, p. 116-126, Mar. 2005.
0271-9142
10.1007/s10875-005-2817-z
1573-2592
url https://www.arca.fiocruz.br/handle/icict/37884
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/37884/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/37884/2/MarizaG_Morgado_etal_IOC_2005.pdf
https://www.arca.fiocruz.br/bitstream/icict/37884/3/MarizaG_Morgado_etal_IOC_2005.pdf.txt
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