Phenotypic and Genotypic Characterization of Atypical Listeria monocytogenes and Listeria innocua Isolated from Swine Slaughterhouses and Meat Markets

Detalhes bibliográficos
Autor(a) principal: Moreno, Luisa Zanolli
Data de Publicação: 2014
Outros Autores: Paixão, Renata, Gobbi, Debora Dirani Sena de, Raimundo, Daniele Cristine, Ferreira, Thais Sebastiana Porfida, Moreno, Andrea Micke, Hofer, Ernesto, Reis, Cristhiane Moura Falavina dos, Matté, Glavur Rogério, Matté, Maria Helena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10869
Resumo: Universidade de São Paulo. Faculdade de Saúde Pública. Laboratório Prática de Saúde Pública. Faculdade de Medicina Veterinária. Laboratório de Sanidade Suína e Virologia. São Paulo, SP, Brasil.
id CRUZ_6e9462e508cef34ae0b3a048b297400b
oai_identifier_str oai:www.arca.fiocruz.br:icict/10869
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Moreno, Luisa ZanolliPaixão, RenataGobbi, Debora Dirani Sena deRaimundo, Daniele CristineFerreira, Thais Sebastiana PorfidaMoreno, Andrea MickeHofer, ErnestoReis, Cristhiane Moura Falavina dosMatté, Glavur RogérioMatté, Maria Helena2015-06-17T12:05:21Z2015-06-17T12:05:21Z2014MORENO, Luisa Zanolli et al. Phenotypic and Genotypic Characterization of Atypical Listeria monocytogenes and Listeria innocua Isolated from Swine Slaughterhouses and Meat Markets. BioMed Research International, v.20, Article ID 742032, 12p, 2014.2314-6133https://www.arca.fiocruz.br/handle/icict/1086910.1155/2014/742032engHindawi Pùblishing CorporationPhenotypic and Genotypic Characterization of Atypical Listeria monocytogenes and Listeria innocua Isolated from Swine Slaughterhouses and Meat Marketsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade de São Paulo. Faculdade de Saúde Pública. Laboratório Prática de Saúde Pública. Faculdade de Medicina Veterinária. Laboratório de Sanidade Suína e Virologia. São Paulo, SP, Brasil.Universidade de São Paulo. Faculdade de Medicina Veterinária. Laboratório de Sanidade Suína e Virologia. São Paulo, SP, Brasil.Universidade de São Paulo. Faculdade de Medicina Veterinária. Laboratório de Sanidade Suína e Virologia. São Paulo, SP, Brasil.Universidade de São Paulo. Faculdade de Medicina Veterinária. Laboratório de Sanidade Suína e Virologia. São Paulo, SP, Brasil.Universidade de São Paulo. Faculdade de Medicina Veterinária. Laboratório de Sanidade Suína e Virologia. São Paulo, SP, Brasil.Universidade de São Paulo. Faculdade de Medicina Veterinária. Laboratório de Sanidade Suína e Virologia. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas, Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas, Rio de Janeiro, RJ, Brasil.Universidade de São Paulo. Faculdade de Saúde Pública. Laboratório Prática de Saúde Pública. São Paulo, SP, Brasil.Universidade de São Paulo. Faculdade de Saúde Pública. Laboratório Prática de Saúde Pública. São Paulo, SP, Brasil.In the last decade, atypical Listeria monocytogenes and L. innocua strains have been detected in food and the environment. Because of mutations in the major virulence genes, these strains have different virulence intensities in eukaryotic cells. In this study, we performed phenotypic and genotypic characterization of atypical L. monocytogenes and L. innocua isolates obtained from swine slaughterhouses and meat markets. Forty strains were studied, including isolates of L. monocytogenes and L. innocua with low-hemolytic activity. The isolates were characterized using conventional phenotypic Listeria identification tests and by the detection and analysis of L. monocytogenes-specific genes. Analysis of 16S rRNA was used for the molecular identification of the Listeria species. The L. monocytogenes isolates were positive for all of the virulence genes studied. The atypical L. innocua strains were positive for hly, plcA, and inlC. Mutations in the InlC, InlB, InlA, PI-PLC, PC-PLC, and PrfA proteins were detected in the atypical isolates. Further in vitro and transcriptomic studies are being developed to confirm the role of these mutations in Listeria virulence.Listeria monocytogenesListeria innocuaListeriaSuínosVirulênciaDoenças dos SuínosMatadourosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10869/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALcristhiane_reisetal_IOC_2014.pdfapplication/pdf1395482https://www.arca.fiocruz.br/bitstream/icict/10869/2/cristhiane_reisetal_IOC_2014.pdfa489c60b6b4c13da2fef70955f3a9d03MD52TEXTcristhiane_reisetal_IOC_2014.pdf.txtcristhiane_reisetal_IOC_2014.pdf.txtExtracted texttext/plain60285https://www.arca.fiocruz.br/bitstream/icict/10869/3/cristhiane_reisetal_IOC_2014.pdf.txtdb21a130a8333dff561c83b12f14f5d8MD53icict/108692022-06-24 13:08:49.28oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T16:08:49Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Phenotypic and Genotypic Characterization of Atypical Listeria monocytogenes and Listeria innocua Isolated from Swine Slaughterhouses and Meat Markets
title Phenotypic and Genotypic Characterization of Atypical Listeria monocytogenes and Listeria innocua Isolated from Swine Slaughterhouses and Meat Markets
spellingShingle Phenotypic and Genotypic Characterization of Atypical Listeria monocytogenes and Listeria innocua Isolated from Swine Slaughterhouses and Meat Markets
Moreno, Luisa Zanolli
Listeria monocytogenes
Listeria innocua
Listeria
Suínos
Virulência
Doenças dos Suínos
Matadouros
title_short Phenotypic and Genotypic Characterization of Atypical Listeria monocytogenes and Listeria innocua Isolated from Swine Slaughterhouses and Meat Markets
title_full Phenotypic and Genotypic Characterization of Atypical Listeria monocytogenes and Listeria innocua Isolated from Swine Slaughterhouses and Meat Markets
title_fullStr Phenotypic and Genotypic Characterization of Atypical Listeria monocytogenes and Listeria innocua Isolated from Swine Slaughterhouses and Meat Markets
title_full_unstemmed Phenotypic and Genotypic Characterization of Atypical Listeria monocytogenes and Listeria innocua Isolated from Swine Slaughterhouses and Meat Markets
title_sort Phenotypic and Genotypic Characterization of Atypical Listeria monocytogenes and Listeria innocua Isolated from Swine Slaughterhouses and Meat Markets
author Moreno, Luisa Zanolli
author_facet Moreno, Luisa Zanolli
Paixão, Renata
Gobbi, Debora Dirani Sena de
Raimundo, Daniele Cristine
Ferreira, Thais Sebastiana Porfida
Moreno, Andrea Micke
Hofer, Ernesto
Reis, Cristhiane Moura Falavina dos
Matté, Glavur Rogério
Matté, Maria Helena
author_role author
author2 Paixão, Renata
Gobbi, Debora Dirani Sena de
Raimundo, Daniele Cristine
Ferreira, Thais Sebastiana Porfida
Moreno, Andrea Micke
Hofer, Ernesto
Reis, Cristhiane Moura Falavina dos
Matté, Glavur Rogério
Matté, Maria Helena
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Moreno, Luisa Zanolli
Paixão, Renata
Gobbi, Debora Dirani Sena de
Raimundo, Daniele Cristine
Ferreira, Thais Sebastiana Porfida
Moreno, Andrea Micke
Hofer, Ernesto
Reis, Cristhiane Moura Falavina dos
Matté, Glavur Rogério
Matté, Maria Helena
dc.subject.en.pt_BR.fl_str_mv Listeria monocytogenes
Listeria innocua
topic Listeria monocytogenes
Listeria innocua
Listeria
Suínos
Virulência
Doenças dos Suínos
Matadouros
dc.subject.decs.pt_BR.fl_str_mv Listeria
Suínos
Virulência
Doenças dos Suínos
Matadouros
description Universidade de São Paulo. Faculdade de Saúde Pública. Laboratório Prática de Saúde Pública. Faculdade de Medicina Veterinária. Laboratório de Sanidade Suína e Virologia. São Paulo, SP, Brasil.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-06-17T12:05:21Z
dc.date.available.fl_str_mv 2015-06-17T12:05:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MORENO, Luisa Zanolli et al. Phenotypic and Genotypic Characterization of Atypical Listeria monocytogenes and Listeria innocua Isolated from Swine Slaughterhouses and Meat Markets. BioMed Research International, v.20, Article ID 742032, 12p, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10869
dc.identifier.issn.pt_BR.fl_str_mv 2314-6133
dc.identifier.doi.pt_BR.fl_str_mv 10.1155/2014/742032
identifier_str_mv MORENO, Luisa Zanolli et al. Phenotypic and Genotypic Characterization of Atypical Listeria monocytogenes and Listeria innocua Isolated from Swine Slaughterhouses and Meat Markets. BioMed Research International, v.20, Article ID 742032, 12p, 2014.
2314-6133
10.1155/2014/742032
url https://www.arca.fiocruz.br/handle/icict/10869
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Hindawi Pùblishing Corporation
publisher.none.fl_str_mv Hindawi Pùblishing Corporation
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/10869/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/10869/2/cristhiane_reisetal_IOC_2014.pdf
https://www.arca.fiocruz.br/bitstream/icict/10869/3/cristhiane_reisetal_IOC_2014.pdf.txt
bitstream.checksum.fl_str_mv 7d48279ffeed55da8dfe2f8e81f3b81f
a489c60b6b4c13da2fef70955f3a9d03
db21a130a8333dff561c83b12f14f5d8
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798324745692774400

Registros relacionados