Sphygmomanometers and thermometers as potential fomites of Staphylococcus haemolyticus: biofilm formation in the presence of antibiotics
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/18250 http://dx.doi.org/10.1590/0074-02760160381 |
Resumo: | Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Rio de Janeiro, RJ, Brasil. |
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Sued, Bruna Pinto RibeiroPereira, Paula Marcele AfonsoFaria, Yuri VieiraRamos, Juliana NunesBinatti, Vanessa BatistaSantos, Kátia Regina Netto dosSeabra, Sérgio HenriqueHirata, RaphaelVieira, Verônica VianaMattos-Guaraldi, Ana LuízaPereira, José Augusto Adler2017-04-03T17:28:13Z2017-04-03T17:28:13Z2017SUED, B. P. R. et al. Sphygmomanometers and thermometers as potential fomites of Staphylococcus haemolyticus: biofilm formation in the presence of antibiotics. Mem. Inst. Oswaldo Cruz, Rio de Janeiro, v. 112, n. 3, p.188-195, 2017.0074-0276https://www.arca.fiocruz.br/handle/icict/18250http://dx.doi.org/10.1590/0074-02760160381engInstituto Oswaldo CruzStaphylococcus haemolyticusFômitesOxacilinaVancomicinaBiofilmesEletroforese em Gel de Campo PulsadoStaphylococcus haemolyticusFomitesOxacillinVancomycinBiofilmPulsed-field gel electrophoresisStaphylococcus haemolyticusFômitesOxacilinaVancomicinaBiofilmesEletroforese em Gel de Campo PulsadoSphygmomanometers and thermometers as potential fomites of Staphylococcus haemolyticus: biofilm formation in the presence of antibioticsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.Centro Universitário Estadual da Zona Oeste. Laboratório de Tecnologia em Bioquímica e Microscopia. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Rio de Janeiro, RJ, Brasil.BACKGROUND: The association between Staphylococcus haemolyticus and severe nosocomial infections is increasing. However, the extent to which fomites contribute to the dissemination of this pathogen through patients and hospital wards remains unknown. OBJECTIVES: In the present study, sphygmomanometers and thermometers were evaluated as potential fomites of oxacillin-resistant S. haemolyticus (ORSH). The influence of oxacillin and vancomycin on biofilm formation by ORSH strains isolated from fomites was also investigated. METHODS: The presence of ORSH on swabs taken from fomite surfaces in a Brazilian hospital was assessed using standard microbiological procedures. Antibiotic susceptibility profiles were determined by the disk diffusion method, and clonal distribution was assessed in pulsed-field gel electrophoresis (PFGE) assays. Minimum inhibitory concentrations (MICs) of oxacillin and vancomycin were evaluated via the broth microdilution method. Polymerase chain reaction (PCR) assays were performed to detect the mecA and icaAD genes. ORSH strains grown in media containing 1/4 MIC of vancomycin or oxacillin were investigated for slime production and biofilm formation on glass, polystyrene and polyurethane catheter surfaces. FINDINGS: ORSH strains comprising five distinct PFGE types were isolated from sphygmomanometers (n = 5) and a thermometer (n = 1) used in intensive care units and surgical wards. ORSH strains isolated from fomites showed susceptibility to only linezolid and vancomycin and were characterised as multi-drug resistant (MDR). Slime production, biofilm formation and the survival of sessile bacteria differed and were independent of the presence of the icaAD and mecA genes, PFGE type and subtype. Vancomycin and oxacillin did not inhibit biofilm formation by vancomycin-susceptible ORSH strains on abiotic surfaces, including on the catheter surface. Enhanced biofilm formation was observed in some situations. Moreover, a sub-lethal dose of vancomycin induced biofilm formation by an ORSH strain on polystyrene. MAIN CONCLUSIONS: Sphygmomanometers and thermometers are fomites for the transmission of ORSH. A sub-lethal dose of vancomycin may favor biofilm formation by ORSH on fomites and catheter surfaces.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/18250/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMem_Inst_Oswaldo_Cruz_112_188-195.pdfMem_Inst_Oswaldo_Cruz_112_188-195.pdfapplication/pdf589044https://www.arca.fiocruz.br/bitstream/icict/18250/2/Mem_Inst_Oswaldo_Cruz_112_188-195.pdfb296b32ad50691eef2f1bbb7ee652dbeMD52TEXTMem_Inst_Oswaldo_Cruz_112_188-195.pdf.txtMem_Inst_Oswaldo_Cruz_112_188-195.pdf.txtExtracted texttext/plain46444https://www.arca.fiocruz.br/bitstream/icict/18250/3/Mem_Inst_Oswaldo_Cruz_112_188-195.pdf.txtb360e120d4beb2160a56584cef39d3b0MD53icict/182502018-08-15 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| dc.title.pt_BR.fl_str_mv |
Sphygmomanometers and thermometers as potential fomites of Staphylococcus haemolyticus: biofilm formation in the presence of antibiotics |
| title |
Sphygmomanometers and thermometers as potential fomites of Staphylococcus haemolyticus: biofilm formation in the presence of antibiotics |
| spellingShingle |
Sphygmomanometers and thermometers as potential fomites of Staphylococcus haemolyticus: biofilm formation in the presence of antibiotics Sued, Bruna Pinto Ribeiro Staphylococcus haemolyticus Fômites Oxacilina Vancomicina Biofilmes Eletroforese em Gel de Campo Pulsado Staphylococcus haemolyticus Fomites Oxacillin Vancomycin Biofilm Pulsed-field gel electrophoresis Staphylococcus haemolyticus Fômites Oxacilina Vancomicina Biofilmes Eletroforese em Gel de Campo Pulsado |
| title_short |
Sphygmomanometers and thermometers as potential fomites of Staphylococcus haemolyticus: biofilm formation in the presence of antibiotics |
| title_full |
Sphygmomanometers and thermometers as potential fomites of Staphylococcus haemolyticus: biofilm formation in the presence of antibiotics |
| title_fullStr |
Sphygmomanometers and thermometers as potential fomites of Staphylococcus haemolyticus: biofilm formation in the presence of antibiotics |
| title_full_unstemmed |
Sphygmomanometers and thermometers as potential fomites of Staphylococcus haemolyticus: biofilm formation in the presence of antibiotics |
| title_sort |
Sphygmomanometers and thermometers as potential fomites of Staphylococcus haemolyticus: biofilm formation in the presence of antibiotics |
| author |
Sued, Bruna Pinto Ribeiro |
| author_facet |
Sued, Bruna Pinto Ribeiro Pereira, Paula Marcele Afonso Faria, Yuri Vieira Ramos, Juliana Nunes Binatti, Vanessa Batista Santos, Kátia Regina Netto dos Seabra, Sérgio Henrique Hirata, Raphael Vieira, Verônica Viana Mattos-Guaraldi, Ana Luíza Pereira, José Augusto Adler |
| author_role |
author |
| author2 |
Pereira, Paula Marcele Afonso Faria, Yuri Vieira Ramos, Juliana Nunes Binatti, Vanessa Batista Santos, Kátia Regina Netto dos Seabra, Sérgio Henrique Hirata, Raphael Vieira, Verônica Viana Mattos-Guaraldi, Ana Luíza Pereira, José Augusto Adler |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Sued, Bruna Pinto Ribeiro Pereira, Paula Marcele Afonso Faria, Yuri Vieira Ramos, Juliana Nunes Binatti, Vanessa Batista Santos, Kátia Regina Netto dos Seabra, Sérgio Henrique Hirata, Raphael Vieira, Verônica Viana Mattos-Guaraldi, Ana Luíza Pereira, José Augusto Adler |
| dc.subject.other.pt_BR.fl_str_mv |
Staphylococcus haemolyticus Fômites Oxacilina Vancomicina Biofilmes Eletroforese em Gel de Campo Pulsado |
| topic |
Staphylococcus haemolyticus Fômites Oxacilina Vancomicina Biofilmes Eletroforese em Gel de Campo Pulsado Staphylococcus haemolyticus Fomites Oxacillin Vancomycin Biofilm Pulsed-field gel electrophoresis Staphylococcus haemolyticus Fômites Oxacilina Vancomicina Biofilmes Eletroforese em Gel de Campo Pulsado |
| dc.subject.en.pt_BR.fl_str_mv |
Staphylococcus haemolyticus Fomites Oxacillin Vancomycin Biofilm Pulsed-field gel electrophoresis |
| dc.subject.decs.pt_BR.fl_str_mv |
Staphylococcus haemolyticus Fômites Oxacilina Vancomicina Biofilmes Eletroforese em Gel de Campo Pulsado |
| description |
Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Rio de Janeiro, RJ, Brasil. |
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2017 |
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2017-04-03T17:28:13Z |
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2017-04-03T17:28:13Z |
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2017 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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SUED, B. P. R. et al. Sphygmomanometers and thermometers as potential fomites of Staphylococcus haemolyticus: biofilm formation in the presence of antibiotics. Mem. Inst. Oswaldo Cruz, Rio de Janeiro, v. 112, n. 3, p.188-195, 2017. |
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https://www.arca.fiocruz.br/handle/icict/18250 |
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0074-0276 |
| dc.identifier.doi.none.fl_str_mv |
http://dx.doi.org/10.1590/0074-02760160381 |
| identifier_str_mv |
SUED, B. P. R. et al. Sphygmomanometers and thermometers as potential fomites of Staphylococcus haemolyticus: biofilm formation in the presence of antibiotics. Mem. Inst. Oswaldo Cruz, Rio de Janeiro, v. 112, n. 3, p.188-195, 2017. 0074-0276 |
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https://www.arca.fiocruz.br/handle/icict/18250 http://dx.doi.org/10.1590/0074-02760160381 |
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Instituto Oswaldo Cruz |
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Instituto Oswaldo Cruz |
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