Biomarker analysis revealed distinct profiles of innate and adaptive immunity in infants with ocular lesions of congenital toxoplasmosis.
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/9812 |
Resumo: | Universidade Federal de Minas Gerais. Departamento de Parasitologia.Belo Horizonte, MG, Brazil |
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Machado, Anderson SilvaCarneiro, Ana Carolina Aguiar VasconcelosBéla, Samantha RibeiroAndrade, Gláucia Manzan QueirozSantos, Daniel Vitor VasconcelosJanuário, José NélioReis, Jordana Grazziela Alves Coelho dosFerro, Eloisa Amália VieiraCarvalho, Andréa TeixeiraVitor, Ricardo Wagner AlmeidaMartins Filho, Olindo Assis2015-03-23T19:05:59Z2015-03-23T19:05:59Z2014MACHADO, Anderson Silva et al. Biomarker analysis revealed distinct profiles of innate and adaptive immunity in infants with ocular lesions of congenital toxoplasmosis. Mediators Inflamm, v. 2014, p. 910621, 20140962-9351https://www.arca.fiocruz.br/handle/icict/981210.1155/2014/910621.engHindawi Pub. CorpBiomarker analysis revealed distinct profiles of innate and adaptive immunity in infants with ocular lesions of congenital toxoplasmosis.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Departamento de Parasitologia.Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Departamento de Parasitologia.Belo Horizonte, MG, BrazilFundaçao Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Biomarcadores de Diagnostico e Monitoraçao. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Departamento de Pediatria. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Oftalmologia e Otorrinolaringologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Nucleo de Acoes e Pesquisa em Apoio Diagnostico. Belo Horizonte, MG, BrazilFundaçao Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Biomarcadores de Diagnostico e Monitoraçao. Belo Horizonte, MG, BrazilUniversidade Federal de Uberlandia. Uberlandia, MG, BrazilFundaçao Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Biomarcadores de Diagnostico e Monitoraçao. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Departamento de Parasitologia.Belo Horizonte, MG, BrazilFundaçao Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Biomarcadores de Diagnostico e Monitoraçao. Belo Horizonte, MG, Brazil /Universidade Federal de Minas Gerais. Congenital Toxoplasmosis Brazilian GroupToxoplasma gondii is the main infectious cause of human posterior retinochoroiditis, the most frequent clinical manifestation of congenital toxoplasmosis. This investigation was performed after neonatal screening to identify biomarkers of immunity associated with immunopathological features of the disease by flow cytometry. The study included infected infants without NRL and with retinochoroidal lesions (ARL, ACRL, and CRL) as well as noninfected individuals (NI). Our data demonstrated that leukocytosis, with increased monocytes and lymphocytes, was a relevant hematological biomarker of ARL. Immunophenotypic analysis also revealed expansion of CD14(+)CD16(+)HLA-DR(high) monocytes and CD56(dim) cytotoxic NK-cells in ARL. Moreover, augmented TCRγ δ (+) and CD8(+) T-cell counts were apparently good biomarkers of morbidity. Biomarker network analysis revealed that complex and intricated networks underscored the negative correlation of monocytes with NK- and B-cells in NRL. The remarkable lack of connections involving B-cells and a relevant shift of NK-cell connections from B-cells toward T-cells observed in ARL were outstanding. A tightly connected biomarker network was observed in CRL, with relevant connections of NK- and CD8(+) T-cells with a broad range of cell subsets. Our findings add novel elements to the current knowledge on the innate and adaptive immune responses in congenital toxoplasmosisToxoplasma gondiiCongenital Toxoplasmosisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82352https://www.arca.fiocruz.br/bitstream/icict/9812/1/license.txtafbdf7d7a9bcf771a1cc7edf5f618413MD51ORIGINAL2014_164.pdf2014_164.pdfapplication/pdf5781303https://www.arca.fiocruz.br/bitstream/icict/9812/2/2014_164.pdf0d199cc6bc3f8fbba3e21f9c870cb8f0MD52TEXT2014_164.pdf.txt2014_164.pdf.txtExtracted texttext/plain53676https://www.arca.fiocruz.br/bitstream/icict/9812/3/2014_164.pdf.txtae9d6aa2937f1325db81bcd6977ed305MD53icict/98122019-06-19 10:12:06.241oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-06-19T13:12:06Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Biomarker analysis revealed distinct profiles of innate and adaptive immunity in infants with ocular lesions of congenital toxoplasmosis. |
| title |
Biomarker analysis revealed distinct profiles of innate and adaptive immunity in infants with ocular lesions of congenital toxoplasmosis. |
| spellingShingle |
Biomarker analysis revealed distinct profiles of innate and adaptive immunity in infants with ocular lesions of congenital toxoplasmosis. Machado, Anderson Silva Toxoplasma gondii Congenital Toxoplasmosis |
| title_short |
Biomarker analysis revealed distinct profiles of innate and adaptive immunity in infants with ocular lesions of congenital toxoplasmosis. |
| title_full |
Biomarker analysis revealed distinct profiles of innate and adaptive immunity in infants with ocular lesions of congenital toxoplasmosis. |
| title_fullStr |
Biomarker analysis revealed distinct profiles of innate and adaptive immunity in infants with ocular lesions of congenital toxoplasmosis. |
| title_full_unstemmed |
Biomarker analysis revealed distinct profiles of innate and adaptive immunity in infants with ocular lesions of congenital toxoplasmosis. |
| title_sort |
Biomarker analysis revealed distinct profiles of innate and adaptive immunity in infants with ocular lesions of congenital toxoplasmosis. |
| author |
Machado, Anderson Silva |
| author_facet |
Machado, Anderson Silva Carneiro, Ana Carolina Aguiar Vasconcelos Béla, Samantha Ribeiro Andrade, Gláucia Manzan Queiroz Santos, Daniel Vitor Vasconcelos Januário, José Nélio Reis, Jordana Grazziela Alves Coelho dos Ferro, Eloisa Amália Vieira Carvalho, Andréa Teixeira Vitor, Ricardo Wagner Almeida Martins Filho, Olindo Assis |
| author_role |
author |
| author2 |
Carneiro, Ana Carolina Aguiar Vasconcelos Béla, Samantha Ribeiro Andrade, Gláucia Manzan Queiroz Santos, Daniel Vitor Vasconcelos Januário, José Nélio Reis, Jordana Grazziela Alves Coelho dos Ferro, Eloisa Amália Vieira Carvalho, Andréa Teixeira Vitor, Ricardo Wagner Almeida Martins Filho, Olindo Assis |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Machado, Anderson Silva Carneiro, Ana Carolina Aguiar Vasconcelos Béla, Samantha Ribeiro Andrade, Gláucia Manzan Queiroz Santos, Daniel Vitor Vasconcelos Januário, José Nélio Reis, Jordana Grazziela Alves Coelho dos Ferro, Eloisa Amália Vieira Carvalho, Andréa Teixeira Vitor, Ricardo Wagner Almeida Martins Filho, Olindo Assis |
| dc.subject.en.pt_BR.fl_str_mv |
Toxoplasma gondii Congenital Toxoplasmosis |
| topic |
Toxoplasma gondii Congenital Toxoplasmosis |
| description |
Universidade Federal de Minas Gerais. Departamento de Parasitologia.Belo Horizonte, MG, Brazil |
| publishDate |
2014 |
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2014 |
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2015-03-23T19:05:59Z |
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2015-03-23T19:05:59Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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MACHADO, Anderson Silva et al. Biomarker analysis revealed distinct profiles of innate and adaptive immunity in infants with ocular lesions of congenital toxoplasmosis. Mediators Inflamm, v. 2014, p. 910621, 2014 |
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https://www.arca.fiocruz.br/handle/icict/9812 |
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0962-9351 |
| dc.identifier.doi.none.fl_str_mv |
10.1155/2014/910621. |
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MACHADO, Anderson Silva et al. Biomarker analysis revealed distinct profiles of innate and adaptive immunity in infants with ocular lesions of congenital toxoplasmosis. Mediators Inflamm, v. 2014, p. 910621, 2014 0962-9351 10.1155/2014/910621. |
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