Blautia and Prevotella sequences distinguish human and animal fecal pollution in Brazil surface waters.
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/10376 |
Resumo: | University of Wisconsin. School of Freshwater Sciences. Milwaukee, Milwaukee, USA |
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Koskey, Amber MFisher, Jenny CEren, A. MuratTerashima, Rafael PonceReis, Mitermayer Galvão dosBlanton, Ronald EdwardMcLellan, Sandra L2015-05-15T18:36:55Z2015-05-15T18:36:55Z2014KOSKEY, A. M. et al. Blautia and Prevotella sequences distinguish human and animal fecal pollution in Brazil surface waters.Environmental Microbiology Reports, v. 6, n. 6, p. 696-704, 2014.1758-2229https://www.arca.fiocruz.br/handle/icict/1037610.1111/1758-2229.12189engWileyBacteroidesPrevotellaBlautiaHumanosPoluiçãoAnimaisFezesEsgotosBrazilBlautia and Prevotella sequences distinguish human and animal fecal pollution in Brazil surface waters.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversity of Wisconsin. School of Freshwater Sciences. Milwaukee, Milwaukee, USAUniversity of Wisconsin. School of Freshwater Sciences. Milwaukee, Milwaukee, USAMarine Biological Laboratory. The Josephine Bay Paul Center. Woods Hole, USA.Case Western Reserve University. Center for Global Health and Diseases. Cleveland, USA.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil / Social Changes. Asthma and Allergy in Latin America. SCAALA. Salvador, BA, BrasilMarine Biological Laboratory. The Josephine Bay Paul Center. Woods Hole, USA.University of Wisconsin. School of Freshwater Sciences. Milwaukee, Milwaukee, USAUntreated sewage discharges and limited agricultural manure management practices contribute to fecal pollution in rural Brazilian waterways. Most microbial source tracking studies have focused on Bacteroidales, and few have tested host-specific indicators in underdeveloped regions. Sequencing of sewage and human and animal feces with Illumina HiSeq revealed Prevotellaceae as the most abundant family in humans, with Lachnospiraceae and Ruminococcaceae also comprising a large proportion of the microbiome. These same families were also dominant in animals. Bacteroides, the genus containing the most commonly utilized human-specific marker in the United States was present in very low abundance. We used oligotyping to identify Prevotella and Blautia sequences that can distinguish human fecal contamination. Thirty-five of 61 Blautia oligotypes and 13 of 108 Prevotella oligotypes in humans were host-specific or highly abundant (i.e. host-preferred) compared to pig, dog, horse and cow sources. Certain human Prevotella and Blautia oligotypes increased more than an order of magnitude along a polluted river transect in rural Brazil, but traditional fecal indicator levels followed a steady or even decreasing trend. While both Prevotella and Blautia oligotypes distinguished human and animal fecal pollution in Brazil surface waters, Blautia appears to contain more discriminatory and globally applicable markers for tracking sources of fecal pollutioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82352https://www.arca.fiocruz.br/bitstream/icict/10376/1/license.txtafbdf7d7a9bcf771a1cc7edf5f618413MD51ORIGINALKoskey AM Blautia....pdfKoskey AM Blautia....pdfapplication/pdf540078https://www.arca.fiocruz.br/bitstream/icict/10376/2/Koskey%20AM%20Blautia....pdfdfdb5e8858900e9fbdc2a04b5e63214fMD52TEXTKoskey AM Blautia....pdf.txtKoskey AM Blautia....pdf.txtExtracted texttext/plain42642https://www.arca.fiocruz.br/bitstream/icict/10376/3/Koskey%20AM%20Blautia....pdf.txtc99323cec5cf404bc3a8c1eb2c5c5530MD53icict/103762023-03-15 14:33:09.173oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:09Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.pt_BR.fl_str_mv |
Blautia and Prevotella sequences distinguish human and animal fecal pollution in Brazil surface waters. |
title |
Blautia and Prevotella sequences distinguish human and animal fecal pollution in Brazil surface waters. |
spellingShingle |
Blautia and Prevotella sequences distinguish human and animal fecal pollution in Brazil surface waters. Koskey, Amber M Bacteroides Prevotella Blautia Humanos Poluição Animais Fezes Esgotos Brazil |
title_short |
Blautia and Prevotella sequences distinguish human and animal fecal pollution in Brazil surface waters. |
title_full |
Blautia and Prevotella sequences distinguish human and animal fecal pollution in Brazil surface waters. |
title_fullStr |
Blautia and Prevotella sequences distinguish human and animal fecal pollution in Brazil surface waters. |
title_full_unstemmed |
Blautia and Prevotella sequences distinguish human and animal fecal pollution in Brazil surface waters. |
title_sort |
Blautia and Prevotella sequences distinguish human and animal fecal pollution in Brazil surface waters. |
author |
Koskey, Amber M |
author_facet |
Koskey, Amber M Fisher, Jenny C Eren, A. Murat Terashima, Rafael Ponce Reis, Mitermayer Galvão dos Blanton, Ronald Edward McLellan, Sandra L |
author_role |
author |
author2 |
Fisher, Jenny C Eren, A. Murat Terashima, Rafael Ponce Reis, Mitermayer Galvão dos Blanton, Ronald Edward McLellan, Sandra L |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Koskey, Amber M Fisher, Jenny C Eren, A. Murat Terashima, Rafael Ponce Reis, Mitermayer Galvão dos Blanton, Ronald Edward McLellan, Sandra L |
dc.subject.other.pt_BR.fl_str_mv |
Bacteroides Prevotella Blautia Humanos Poluição Animais Fezes Esgotos Brazil |
topic |
Bacteroides Prevotella Blautia Humanos Poluição Animais Fezes Esgotos Brazil |
description |
University of Wisconsin. School of Freshwater Sciences. Milwaukee, Milwaukee, USA |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014 |
dc.date.accessioned.fl_str_mv |
2015-05-15T18:36:55Z |
dc.date.available.fl_str_mv |
2015-05-15T18:36:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
KOSKEY, A. M. et al. Blautia and Prevotella sequences distinguish human and animal fecal pollution in Brazil surface waters.Environmental Microbiology Reports, v. 6, n. 6, p. 696-704, 2014. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/10376 |
dc.identifier.issn.none.fl_str_mv |
1758-2229 |
dc.identifier.doi.none.fl_str_mv |
10.1111/1758-2229.12189 |
identifier_str_mv |
KOSKEY, A. M. et al. Blautia and Prevotella sequences distinguish human and animal fecal pollution in Brazil surface waters.Environmental Microbiology Reports, v. 6, n. 6, p. 696-704, 2014. 1758-2229 10.1111/1758-2229.12189 |
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https://www.arca.fiocruz.br/handle/icict/10376 |
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Wiley |
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Wiley |
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