Leptospira interrogans insoluble fraction as a potential antigen source for lateral flow immunochromatography

Detalhes bibliográficos
Autor(a) principal: Campos, Hevandro de Souza
Data de Publicação: 2023
Outros Autores: Silva, Edimilson Domingos da, Lima, Gerson Silva de, Resende, Rafael de Oliveira, Burth, Patricia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/57890
Resumo: Universidade Federal Fluminense, Departamento de Biologia Celular e Molecular, Niterói, RJ, Brasil / Universidade Federal Fluminense. Programa de Pós-Graduação em Biotecnologia. Niterói, RJ, Brasil.
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spelling Campos, Hevandro de SouzaSilva, Edimilson Domingos daLima, Gerson Silva deResende, Rafael de OliveiraBurth, Patricia2023-04-19T16:03:09Z2023-04-19T16:03:09Z2023CAMPOS, Hevandro de Souza et al. Leptospira interrogans insoluble fraction as a potential antigen source for lateral flow immunochromatography. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, RJ, Brasil. v. 118, e220265, p. 1 - 6, Apr. 2023.0074-0206https://www.arca.fiocruz.br/handle/icict/5789010.1590/0074-02760220265engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.LeptospiroseFluxo lateralImunocromatografiaDiagnósticoLeptospirosisLateral flowImmunochromatographyDiagnosisLeptospira interrogans insoluble fraction as a potential antigen source for lateral flow immunochromatographyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal Fluminense, Departamento de Biologia Celular e Molecular, Niterói, RJ, Brasil / Universidade Federal Fluminense. Programa de Pós-Graduação em Biotecnologia. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Bio-Manguinhos, Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Bio-Manguinhos, Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Laboratório de Pesquisas sobre o Timo, Rio de Janeiro, RJ, Brasil / Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Uberlândia, MG, Brasil.Universidade Federal Fluminense, Departamento de Biologia Celular e Molecular, Niterói, RJ, Brasil / Universidade Federal Fluminense. Programa de Pós-Graduação em Biotecnologia. Niterói, RJ, Brasil.BACKGROUND Leptospirosis is an emerging zoonosis that affects humans and animals. Immunochromatography rapid test is widely used for early diagnosis of leptospirosis, but with low sensitivity and specificity. OBJECTIVES To evaluate Leptospira interrogans insoluble fraction as a potential antigen source for lateral flow immunochromatography. METHODS Insoluble fraction derived from the crude bacterial extract was obtained by serial centrifugation. The polypeptide profile was determined using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Immune reactivity of this fraction was assessed by Western Blotting and lateral flow immunochromatography (LFI). It was tested 160 microagglutination test (MAT)-positive sera from patients in the acute phase, 100 MAT-negative sera from patients with acute febrile illness, and 45 patients with other infectious diseases. FINDINGS There was a predominance of low molecular mass-polypeptide bands, ranging from 2 to 37 kDa. The antibody reactivity of theses polypeptides was found to range from 13-50%, especially between 10 and 38 kDa. Among MAT-positive sera of patients with leptospirosis in the acute phase, 97% were also positive in LFI, indicating high sensitivity. Among MAT-negative sera, all were negative in LFI, indicating high specificity. Only 2% of cross-reactivity was detected. CONCLUSION The insoluble fraction can be a valuable antigen source for development of point-of-care diagnosis test for leptospirosis.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/57890/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALRaphaelO. Rezende_etal_IOC_2023.pdfRaphaelO. Rezende_etal_IOC_2023.pdfapplication/pdf812112https://www.arca.fiocruz.br/bitstream/icict/57890/2/RaphaelO.%20Rezende_etal_IOC_2023.pdf62d69cf3be0c9376e526cdb1eae038a3MD52icict/578902023-09-04 11:29:58.793oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-04T14:29:58Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv Leptospira interrogans insoluble fraction as a potential antigen source for lateral flow immunochromatography
title Leptospira interrogans insoluble fraction as a potential antigen source for lateral flow immunochromatography
spellingShingle Leptospira interrogans insoluble fraction as a potential antigen source for lateral flow immunochromatography
Campos, Hevandro de Souza
Leptospirose
Fluxo lateral
Imunocromatografia
Diagnóstico
Leptospirosis
Lateral flow
Immunochromatography
Diagnosis
title_short Leptospira interrogans insoluble fraction as a potential antigen source for lateral flow immunochromatography
title_full Leptospira interrogans insoluble fraction as a potential antigen source for lateral flow immunochromatography
title_fullStr Leptospira interrogans insoluble fraction as a potential antigen source for lateral flow immunochromatography
title_full_unstemmed Leptospira interrogans insoluble fraction as a potential antigen source for lateral flow immunochromatography
title_sort Leptospira interrogans insoluble fraction as a potential antigen source for lateral flow immunochromatography
author Campos, Hevandro de Souza
author_facet Campos, Hevandro de Souza
Silva, Edimilson Domingos da
Lima, Gerson Silva de
Resende, Rafael de Oliveira
Burth, Patricia
author_role author
author2 Silva, Edimilson Domingos da
Lima, Gerson Silva de
Resende, Rafael de Oliveira
Burth, Patricia
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Campos, Hevandro de Souza
Silva, Edimilson Domingos da
Lima, Gerson Silva de
Resende, Rafael de Oliveira
Burth, Patricia
dc.subject.other.en_US.fl_str_mv Leptospirose
Fluxo lateral
Imunocromatografia
Diagnóstico
topic Leptospirose
Fluxo lateral
Imunocromatografia
Diagnóstico
Leptospirosis
Lateral flow
Immunochromatography
Diagnosis
dc.subject.en.en_US.fl_str_mv Leptospirosis
Lateral flow
Immunochromatography
Diagnosis
description Universidade Federal Fluminense, Departamento de Biologia Celular e Molecular, Niterói, RJ, Brasil / Universidade Federal Fluminense. Programa de Pós-Graduação em Biotecnologia. Niterói, RJ, Brasil.
publishDate 2023
dc.date.accessioned.fl_str_mv 2023-04-19T16:03:09Z
dc.date.available.fl_str_mv 2023-04-19T16:03:09Z
dc.date.issued.fl_str_mv 2023
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CAMPOS, Hevandro de Souza et al. Leptospira interrogans insoluble fraction as a potential antigen source for lateral flow immunochromatography. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, RJ, Brasil. v. 118, e220265, p. 1 - 6, Apr. 2023.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/57890
dc.identifier.issn.en_US.fl_str_mv 0074-0206
dc.identifier.doi.none.fl_str_mv 10.1590/0074-02760220265
identifier_str_mv CAMPOS, Hevandro de Souza et al. Leptospira interrogans insoluble fraction as a potential antigen source for lateral flow immunochromatography. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, RJ, Brasil. v. 118, e220265, p. 1 - 6, Apr. 2023.
0074-0206
10.1590/0074-02760220265
url https://www.arca.fiocruz.br/handle/icict/57890
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/57890/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/57890/2/RaphaelO.%20Rezende_etal_IOC_2023.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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