A case of sporotrichosis caused by different Sporothrix brasiliensis strains: mycological, molecular, and virulence analyses

Oliveira, Manoel Marques E.; Almeida-Paes, Rodrigo; Corrêa-Moreira, Danielly; Borba, Cintia de Moraes; Menezes, Rodrigo Caldas; Freitas, Dayvison Francis Saraiva; Valle, Antonio Carlos Francesconi do; Schubach, Armando de Oliveira; Barros, Monica Bastos de Lima; Nosanchuk, Joshua D.; Gutierrez-Galhardo, Maria Clara; Zancopé-Oliveira, Rosely Maria

A case of sporotrichosis caused by different Sporothrix brasiliensis strains: mycological, molecular, and virulence analyses

Detalhes bibliográficos
Autor(a) principal:	Oliveira, Manoel Marques E.
Data de Publicação:	2019
Outros Autores:	Almeida-Paes, Rodrigo, Corrêa-Moreira, Danielly, Borba, Cintia de Moraes, Menezes, Rodrigo Caldas, Freitas, Dayvison Francis Saraiva, Valle, Antonio Carlos Francesconi do, Schubach, Armando de Oliveira, Barros, Monica Bastos de Lima, Nosanchuk, Joshua D., Gutierrez-Galhardo, Maria Clara, Zancopé-Oliveira, Rosely Maria
Tipo de documento:	Artigo
Idioma:	eng
Título da fonte:	Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo:	https://www.arca.fiocruz.br/handle/icict/36943
Resumo:	Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil.

Metadados do item

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spelling	Oliveira, Manoel Marques E.Almeida-Paes, RodrigoCorrêa-Moreira, DaniellyBorba, Cintia de MoraesMenezes, Rodrigo CaldasFreitas, Dayvison Francis SaraivaValle, Antonio Carlos Francesconi doSchubach, Armando de OliveiraBarros, Monica Bastos de LimaNosanchuk, Joshua D.Gutierrez-Galhardo, Maria ClaraZancopé-Oliveira, Rosely Maria2019-11-07T15:12:16Z2019-11-07T15:12:16Z2019OLIVEIRA, Manoel Marques E. et al. A case of sporotrichosis caused by different Sporothrix brasiliensis strains: mycological, molecular, and virulence analyses. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 144, p. 1-13, 2019.0074-0276https://www.arca.fiocruz.br/handle/icict/3694310.1590/0074-027601902601678-8060engFiocruz/IOCA case of sporotrichosis caused by different Sporothrix brasiliensis strains: mycological, molecular, and virulence analysesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos, Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasi.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Vigilância em Leishmaniose. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública. Rio de Janeiro, RJ, Brasil.Albert Einstein College of Medicine. New York, NY, USA.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Background: Sporotrichosis is a subcutaneous mycosis caused by dimorphic pathogenic fungi belonging to the Sporothrix genus. Pathogenic Sporothrix species typically produce melanin, which is known to be a virulence factor. Objectives: The aim of this study was to perform phenotypic, genotypic, and virulence analyses of two distinct Sporothrix brasiliensis strains isolated from the same lesion on a patient from Rio de Janeiro. Methods and Findings: Genotypic analyses by partial sequencing of the calmodulin, β-tubulin, and chitin synthase genes, as well as polymerase chain reaction (PCR)-fingerprinting by T3B, M13, and GACA, showed that the isolates were very similar but not identical. Both isolates had similar phenotypic characteristics and effectively produced melanin in their yeast forms, accounting for their ability of causing disease in a murine sporotrichosis model. Remarkably, isolate B was albino in its environmental form but caused more severe disease than the pigmented A isolate. Conclusions: These findings indicate that the patient was infected by two genetically and biologically distinct S. brasiliensis that vary in their production of melanin in their environmental forms. The results underscore the importance of characterizing phenotypically different isolates found in the same clinical specimen or patient.SporothrixGenotypic analysesPCR fingerprintExperimental murine modelVirulenceinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83104https://www.arca.fiocruz.br/bitstream/icict/36943/1/license.txt79178e5f2a0eb066867a274556814938MD51ORIGINALve_Oliveira_Manoel_etal_INI_2019.pdfve_Oliveira_Manoel_etal_INI_2019.pdfapplication/pdf7044495https://www.arca.fiocruz.br/bitstream/icict/36943/2/ve_Oliveira_Manoel_etal_INI_2019.pdf5d2a487ae2c9630c204eeb7ae358281fMD52TEXTve_Oliveira_Manoel_etal_INI_2019.pdf.txtve_Oliveira_Manoel_etal_INI_2019.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv	A case of sporotrichosis caused by different Sporothrix brasiliensis strains: mycological, molecular, and virulence analyses
title	A case of sporotrichosis caused by different Sporothrix brasiliensis strains: mycological, molecular, and virulence analyses
spellingShingle	A case of sporotrichosis caused by different Sporothrix brasiliensis strains: mycological, molecular, and virulence analyses Oliveira, Manoel Marques E. Sporothrix Genotypic analyses PCR fingerprint Experimental murine model Virulence
title_short	A case of sporotrichosis caused by different Sporothrix brasiliensis strains: mycological, molecular, and virulence analyses
title_full	A case of sporotrichosis caused by different Sporothrix brasiliensis strains: mycological, molecular, and virulence analyses
title_fullStr	A case of sporotrichosis caused by different Sporothrix brasiliensis strains: mycological, molecular, and virulence analyses
title_full_unstemmed	A case of sporotrichosis caused by different Sporothrix brasiliensis strains: mycological, molecular, and virulence analyses
title_sort	A case of sporotrichosis caused by different Sporothrix brasiliensis strains: mycological, molecular, and virulence analyses
author	Oliveira, Manoel Marques E.
author_facet	Oliveira, Manoel Marques E. Almeida-Paes, Rodrigo Corrêa-Moreira, Danielly Borba, Cintia de Moraes Menezes, Rodrigo Caldas Freitas, Dayvison Francis Saraiva Valle, Antonio Carlos Francesconi do Schubach, Armando de Oliveira Barros, Monica Bastos de Lima Nosanchuk, Joshua D. Gutierrez-Galhardo, Maria Clara Zancopé-Oliveira, Rosely Maria
author_role	author
author2	Almeida-Paes, Rodrigo Corrêa-Moreira, Danielly Borba, Cintia de Moraes Menezes, Rodrigo Caldas Freitas, Dayvison Francis Saraiva Valle, Antonio Carlos Francesconi do Schubach, Armando de Oliveira Barros, Monica Bastos de Lima Nosanchuk, Joshua D. Gutierrez-Galhardo, Maria Clara Zancopé-Oliveira, Rosely Maria
author2_role	author author author author author author author author author author author
dc.contributor.author.fl_str_mv	Oliveira, Manoel Marques E. Almeida-Paes, Rodrigo Corrêa-Moreira, Danielly Borba, Cintia de Moraes Menezes, Rodrigo Caldas Freitas, Dayvison Francis Saraiva Valle, Antonio Carlos Francesconi do Schubach, Armando de Oliveira Barros, Monica Bastos de Lima Nosanchuk, Joshua D. Gutierrez-Galhardo, Maria Clara Zancopé-Oliveira, Rosely Maria
dc.subject.en.pt_BR.fl_str_mv	Sporothrix Genotypic analyses PCR fingerprint Experimental murine model Virulence
topic	Sporothrix Genotypic analyses PCR fingerprint Experimental murine model Virulence
description	Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil.
publishDate	2019
dc.date.accessioned.fl_str_mv	2019-11-07T15:12:16Z
dc.date.available.fl_str_mv	2019-11-07T15:12:16Z
dc.date.issued.fl_str_mv	2019
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	OLIVEIRA, Manoel Marques E. et al. A case of sporotrichosis caused by different Sporothrix brasiliensis strains: mycological, molecular, and virulence analyses. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 144, p. 1-13, 2019.
dc.identifier.uri.fl_str_mv	https://www.arca.fiocruz.br/handle/icict/36943
dc.identifier.issn.pt_BR.fl_str_mv	0074-0276
dc.identifier.doi.none.fl_str_mv	10.1590/0074-02760190260
dc.identifier.eissn.none.fl_str_mv	1678-8060
identifier_str_mv	OLIVEIRA, Manoel Marques E. et al. A case of sporotrichosis caused by different Sporothrix brasiliensis strains: mycological, molecular, and virulence analyses. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 144, p. 1-13, 2019. 0074-0276 10.1590/0074-02760190260 1678-8060
url	https://www.arca.fiocruz.br/handle/icict/36943
dc.language.iso.fl_str_mv	eng
language	eng
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.publisher.none.fl_str_mv	Fiocruz/IOC
publisher.none.fl_str_mv	Fiocruz/IOC
dc.source.none.fl_str_mv	reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ
instname_str	Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str	FIOCRUZ
institution	FIOCRUZ
reponame_str	Repositório Institucional da FIOCRUZ (ARCA)
collection	Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv	https://www.arca.fiocruz.br/bitstream/icict/36943/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/36943/2/ve_Oliveira_Manoel_etal_INI_2019.pdf https://www.arca.fiocruz.br/bitstream/icict/36943/3/ve_Oliveira_Manoel_etal_INI_2019.pdf.txt
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repository.name.fl_str_mv	Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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A case of sporotrichosis caused by different Sporothrix brasiliensis strains: mycological, molecular, and virulence analyses

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