Lipids From Trypanosoma cruzi Amastigotes of RA and K98 Strains Generate a Pro-inflammatory Response via TLR2/6

Detalhes bibliográficos
Autor(a) principal: Bott, Emanuel
Data de Publicação: 2018
Outros Autores: Carneiro, Alan B., Gimenez, Guadalupe, López, María G., Lammel, Estela M., Atella, Georgia C., Bozza, Patrícia T., Belaunzarán, María L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/29312
Resumo: Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología, Parasitología e Inmunología,. Buenos Aires, Argentina / Universidad de Buenos Aires. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica. Buenos Aires, Argentina.
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spelling Bott, EmanuelCarneiro, Alan B.Gimenez, GuadalupeLópez, María G.Lammel, Estela M.Atella, Georgia C.Bozza, Patrícia T.Belaunzarán, María L.2018-10-04T11:20:29Z2018-10-04T11:20:29Z2018BOTT, Emanuel; et al. Lipids From Trypanosoma cruzi Amastigotes of RA and K98 Strains Generate a Pro-inflammatory Response via TLR2/6. Frontiers in Celluar and Infection Microbiology, v.8, Article 151, 14p, May 2018.2235-2988https://www.arca.fiocruz.br/handle/icict/2931210.3389/fcimb.2018.00151engFrontiers MediaTrypanosoma cruziLipídeospró-inflamatórioFosfolipases A1Trypanosoma cruzilipidspro-inflammatoryphospholipase A1TLR2/6Lipids From Trypanosoma cruzi Amastigotes of RA and K98 Strains Generate a Pro-inflammatory Response via TLR2/6info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología, Parasitología e Inmunología,. Buenos Aires, Argentina / Universidad de Buenos Aires. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica. Buenos Aires, Argentina.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ. Brasil.Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología, Parasitología e Inmunología,. Buenos Aires, Argentina / Universidad de Buenos Aires. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica. Buenos Aires, Argentina.Instituto Nacional de Tecnología Agropecuaria. Instituto de Biotecnología. Buenos Aires, Argentina / Consejo Nacional de Investigaciones Científicas y Técnicas. Buenos Aires, Argentina.Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología, Parasitología e Inmunología,. Buenos Aires, Argentina / Universidad de Buenos Aires. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica. Buenos Aires, Argentina.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ. Brasil.Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología, Parasitología e Inmunología,. Buenos Aires, Argentina / Universidad de Buenos Aires. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica. Buenos Aires, Argentina.Lipids from microorganisms are ligands of Toll like receptors (TLRs) and modulate the innate immune response. Herein, we analyze in vitro the effect of total lipid extracts from Trypanosoma cruzi amastigotes of RA and K98 strains (with polar biological behavior) on the induction of the inflammatory response and the involvement of TLRs in this process. We demonstrated that total lipid extracts from both strains induced lipid body formation, cyclooxygenase-2 expression and TNF-α and nitric oxide release in macrophages, as well as NF-κB activation and IL-8 release in HEK cells specifically through a TLR2/6 dependent pathway. We also evaluated the inflammatory response induced by total lipid extracts obtained from lysed parasites that were overnight incubated to allow the action of parasite hydrolytic enzymes, such as Phospholipase A1, over endogenous phospholipids. After incubation, these total lipid extracts showed a significantly reduced pro-inflammatory response, which could be attributed to the changes in the content of known bioactive lipid molecules like lysophospholipids and fatty acids, here reported. Moreover, analyses of total fatty acids in each lipid extract were performed by gas chromatography-mass spectrometry. Our results indicate a relevant role of T. cruzi lipids in the induction of a pro-inflammatory response through the TLR2/6 pathway that could contribute to the modulation of the immune response and host survival.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/29312/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALalanB_carneiro_etal_IOC_2018.pdfalanB_carneiro_etal_IOC_2018.pdfapplication/pdf2005935https://www.arca.fiocruz.br/bitstream/icict/29312/2/alanB_carneiro_etal_IOC_2018.pdf55686c4406457bbc757661fe684da3e2MD52TEXTalanB_carneiro_etal_IOC_2018.pdf.txtalanB_carneiro_etal_IOC_2018.pdf.txtExtracted texttext/plain65585https://www.arca.fiocruz.br/bitstream/icict/29312/3/alanB_carneiro_etal_IOC_2018.pdf.txt281cb230a9bb32ae6ce22979776c2839MD53icict/293122022-06-24 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dc.title.pt_BR.fl_str_mv Lipids From Trypanosoma cruzi Amastigotes of RA and K98 Strains Generate a Pro-inflammatory Response via TLR2/6
title Lipids From Trypanosoma cruzi Amastigotes of RA and K98 Strains Generate a Pro-inflammatory Response via TLR2/6
spellingShingle Lipids From Trypanosoma cruzi Amastigotes of RA and K98 Strains Generate a Pro-inflammatory Response via TLR2/6
Bott, Emanuel
Trypanosoma cruzi
Lipídeos
pró-inflamatório
Fosfolipases A1
Trypanosoma cruzi
lipids
pro-inflammatory
phospholipase A1
TLR2/6
title_short Lipids From Trypanosoma cruzi Amastigotes of RA and K98 Strains Generate a Pro-inflammatory Response via TLR2/6
title_full Lipids From Trypanosoma cruzi Amastigotes of RA and K98 Strains Generate a Pro-inflammatory Response via TLR2/6
title_fullStr Lipids From Trypanosoma cruzi Amastigotes of RA and K98 Strains Generate a Pro-inflammatory Response via TLR2/6
title_full_unstemmed Lipids From Trypanosoma cruzi Amastigotes of RA and K98 Strains Generate a Pro-inflammatory Response via TLR2/6
title_sort Lipids From Trypanosoma cruzi Amastigotes of RA and K98 Strains Generate a Pro-inflammatory Response via TLR2/6
author Bott, Emanuel
author_facet Bott, Emanuel
Carneiro, Alan B.
Gimenez, Guadalupe
López, María G.
Lammel, Estela M.
Atella, Georgia C.
Bozza, Patrícia T.
Belaunzarán, María L.
author_role author
author2 Carneiro, Alan B.
Gimenez, Guadalupe
López, María G.
Lammel, Estela M.
Atella, Georgia C.
Bozza, Patrícia T.
Belaunzarán, María L.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bott, Emanuel
Carneiro, Alan B.
Gimenez, Guadalupe
López, María G.
Lammel, Estela M.
Atella, Georgia C.
Bozza, Patrícia T.
Belaunzarán, María L.
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
Lipídeos
pró-inflamatório
Fosfolipases A1
topic Trypanosoma cruzi
Lipídeos
pró-inflamatório
Fosfolipases A1
Trypanosoma cruzi
lipids
pro-inflammatory
phospholipase A1
TLR2/6
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
lipids
pro-inflammatory
phospholipase A1
TLR2/6
description Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología, Parasitología e Inmunología,. Buenos Aires, Argentina / Universidad de Buenos Aires. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica. Buenos Aires, Argentina.
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-10-04T11:20:29Z
dc.date.available.fl_str_mv 2018-10-04T11:20:29Z
dc.date.issued.fl_str_mv 2018
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BOTT, Emanuel; et al. Lipids From Trypanosoma cruzi Amastigotes of RA and K98 Strains Generate a Pro-inflammatory Response via TLR2/6. Frontiers in Celluar and Infection Microbiology, v.8, Article 151, 14p, May 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/29312
dc.identifier.issn.pt_BR.fl_str_mv 2235-2988
dc.identifier.doi.none.fl_str_mv 10.3389/fcimb.2018.00151
identifier_str_mv BOTT, Emanuel; et al. Lipids From Trypanosoma cruzi Amastigotes of RA and K98 Strains Generate a Pro-inflammatory Response via TLR2/6. Frontiers in Celluar and Infection Microbiology, v.8, Article 151, 14p, May 2018.
2235-2988
10.3389/fcimb.2018.00151
url https://www.arca.fiocruz.br/handle/icict/29312
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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