Thick smear is a good substitute for the thin smear in parasitological confirmation of canine visceral leishmaniasis

Detalhes bibliográficos
Autor(a) principal: Mello, Cintia Xavier de
Data de Publicação: 2016
Outros Autores: Figueiredo, Fabiano Borges, Mendes Júnior, Artur Augusto Velho, Miranda, Luciana de Freitas Campos, Oliveira, Raquel de Vasconcellos Carvalhaes de, Madeira, Maria de Fátima
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/31877
Resumo: Artigo disponível em acesso aberto em https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4944718/
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spelling Mello, Cintia Xavier deFigueiredo, Fabiano BorgesMendes Júnior, Artur Augusto VelhoMiranda, Luciana de Freitas CamposOliveira, Raquel de Vasconcellos Carvalhaes deMadeira, Maria de Fátima2019-02-26T14:18:49Z2019-02-26T14:18:49Z2016MELLO, Cintia Xavier de et al. Thick smear is a good substitute for the thin smear in parasitological confirmation of canine visceral leishmaniasis. American Journal of Tropical Medicine and Hygiene, v. 95, n. 1, p. 99-103, 2016.0002-9637https://www.arca.fiocruz.br/handle/icict/3187710.4269/ajtmh.15-01031476-1645Artigo disponível em acesso aberto em https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4944718/Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica e Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica e Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Epidemiologia Clínica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica e Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil.Although direct examination methods are important for diagnosing leishmaniasis, such methods are often neglected because of their low sensitivity relative to other techniques. Our study aimed to evaluate the performance of bone marrow (BM) thick smears and cytocentrifugation tests as alternatives to direct examination for diagnosing canine visceral leishmaniasis (CVL). Ninety-two dogs exhibiting leishmaniasis seroreactivity were evaluated. The animals were euthanized; and healthy skin, spleen, popliteal lymph node, and BM puncture samples were cultured. BM cultures were used as the reference standard. Of the 92 dogs studied, 85.9% exhibited positive cultures, and Leishmania infantum (synonym Leishmania chagasi) was confirmed in all positive culture cases. The sensitivity rates for cytocentrifugation as well as thin and thick smears were 47.1%, 52.8%, and 77%, respectively. However, no association between the dogs' clinical status and culture or direct examination results was found. To our knowledge, this was the first study to use thick smears and cytocentrifugation for diagnosing CVL. Our results indicate that BM thick smears have a good sensitivity and their use reduces the time required to read slides. Therefore, thick smears can provide a rapid and safe alternative to parasitological confirmation of seroreactive dogs.engAmerican Society of Tropical Medicine and HygieneThick smear is a good substitute for the thin smear in parasitological confirmation of canine visceral leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCanine visceral leishmaniasisDiagnosisThick smearinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Thick smear is a good substitute for the thin smear in parasitological confirmation of canine visceral leishmaniasis
title Thick smear is a good substitute for the thin smear in parasitological confirmation of canine visceral leishmaniasis
spellingShingle Thick smear is a good substitute for the thin smear in parasitological confirmation of canine visceral leishmaniasis
Mello, Cintia Xavier de
Canine visceral leishmaniasis
Diagnosis
Thick smear
title_short Thick smear is a good substitute for the thin smear in parasitological confirmation of canine visceral leishmaniasis
title_full Thick smear is a good substitute for the thin smear in parasitological confirmation of canine visceral leishmaniasis
title_fullStr Thick smear is a good substitute for the thin smear in parasitological confirmation of canine visceral leishmaniasis
title_full_unstemmed Thick smear is a good substitute for the thin smear in parasitological confirmation of canine visceral leishmaniasis
title_sort Thick smear is a good substitute for the thin smear in parasitological confirmation of canine visceral leishmaniasis
author Mello, Cintia Xavier de
author_facet Mello, Cintia Xavier de
Figueiredo, Fabiano Borges
Mendes Júnior, Artur Augusto Velho
Miranda, Luciana de Freitas Campos
Oliveira, Raquel de Vasconcellos Carvalhaes de
Madeira, Maria de Fátima
author_role author
author2 Figueiredo, Fabiano Borges
Mendes Júnior, Artur Augusto Velho
Miranda, Luciana de Freitas Campos
Oliveira, Raquel de Vasconcellos Carvalhaes de
Madeira, Maria de Fátima
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Mello, Cintia Xavier de
Figueiredo, Fabiano Borges
Mendes Júnior, Artur Augusto Velho
Miranda, Luciana de Freitas Campos
Oliveira, Raquel de Vasconcellos Carvalhaes de
Madeira, Maria de Fátima
dc.subject.en.pt_BR.fl_str_mv Canine visceral leishmaniasis
Diagnosis
Thick smear
topic Canine visceral leishmaniasis
Diagnosis
Thick smear
description Artigo disponível em acesso aberto em https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4944718/
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2019-02-26T14:18:49Z
dc.date.available.fl_str_mv 2019-02-26T14:18:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MELLO, Cintia Xavier de et al. Thick smear is a good substitute for the thin smear in parasitological confirmation of canine visceral leishmaniasis. American Journal of Tropical Medicine and Hygiene, v. 95, n. 1, p. 99-103, 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/31877
dc.identifier.issn.pt_BR.fl_str_mv 0002-9637
dc.identifier.doi.none.fl_str_mv 10.4269/ajtmh.15-0103
dc.identifier.eissn.none.fl_str_mv 1476-1645
identifier_str_mv MELLO, Cintia Xavier de et al. Thick smear is a good substitute for the thin smear in parasitological confirmation of canine visceral leishmaniasis. American Journal of Tropical Medicine and Hygiene, v. 95, n. 1, p. 99-103, 2016.
0002-9637
10.4269/ajtmh.15-0103
1476-1645
url https://www.arca.fiocruz.br/handle/icict/31877
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society of Tropical Medicine and Hygiene
publisher.none.fl_str_mv American Society of Tropical Medicine and Hygiene
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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