Proteomics study of human cord blood reticulocyte-derived exosomes
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/36904 |
Resumo: | Universidade de Barcelona. Hospital Clínic. ISGlobal. Barcelona, Espanha. |
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Díaz-Varela, MíriamMenezes-Neto, Armando dePerez-Zsolt, DanielGámez-Valero, AnaSeguí-Barber, JoanIzquierdo-Useros, NuriaMartinez-Picado, JavierFernández-Becerra, CarmenPortillo, Hernando A. del2019-11-06T15:09:17Z2019-11-06T15:09:17Z2018DÍAZ-VARELA, M. et al. Proteomics study of human cord blood reticulocyte-derived exosomes. Scientific Reports, v. 8, n. 1, p. 1-11, 2018.2045-2322https://www.arca.fiocruz.br/handle/icict/3690410.1038/s41598-018-32386-2engProteomics study of human cord blood reticulocyte-derived exosomesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade de Barcelona. Hospital Clínic. ISGlobal. Barcelona, Espanha.Universidade de Barcelona. Hospital Clínic. ISGlobal. Barcelona, Espanha / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Instituto de Pesquisa em AIDS IrsiCaixa. Badalona, Espanha.Universidade de Barcelona. Hospital Clínic. ISGlobal. Barcelona, Espanha / Universitat Autònoma de Barcelona. Hospital Universitário e Instituto de Pesquisa em Ciências da Saúde Germans Trias i Pujol. Departamento de Patologia e Grupo REMAR-IVECAT. Barcelona, Espanha.Universidade de Barcelona. Hospital Clínic. ISGlobal. Barcelona, Espanha.Instituto de Pesquisa em AIDS IrsiCaixa. Badalona, Espanha / Instituto IGTP de Investigação Alemão Trias i Pujol. Badalona, Espanha.Instituto de Pesquisa em AIDS IrsiCaixa. Badalona, Espanha / Universitat de Vic - Universidade Central da Catalunha. Vic, Espanha / Instituto Catalão de Estudos e Estudos Avançados. Barcelona, Espanha.ISGlobal, Hospital Clínic - Universidade de Barcelona. Barcelona, Espanha / Instituto IGTP de Investigação Alemão Trias i Pujol. Badalona, Espanha.ISGlobal, Hospital Clínic - Universidade de Barcelona. Barcelona, Espanha / Instituto IGTP de Investigação Alemão Trias i Pujol. Badalona, Espanha / Instituto Catalão de Estudos e Estudos Avançados. Barcelona, Espanha.Reticulocyte-derived exosomes (Rex), extracellular vesicles of endocytic origin, were initially discovered as a cargo-disposal mechanism of obsolete proteins in the maturation of reticulocytes into erythrocytes. In this work, we present the first mass spectrometry-based proteomics of human Rex (HuRex). HuRex were isolated from cultures of human reticulocyte-enriched cord blood using different culture conditions and exosome isolation methods. The newly described proteome consists of 367 proteins, most of them related to exosomes as revealed by gene ontology over-representation analysis and include multiple transporters as well as proteins involved in exosome biogenesis and erythrocytic disorders. Immunoelectron microscopy validated the presence of the transferrin receptor. Moreover, functional assays demonstrated active capture of HuRex by mature dendritic cells. As only seven proteins have been previously associated with HuRex, this resource will facilitate studies on the role of human reticulocyte-derived exosomes in normal and pathological conditions affecting erythropoiesis.ProteomicsHuman cord bloodReticulocyte-derived exosomesBancos de SangueTécnicas de Cultura de CélulasCélulas CultivadasCélulas Dendríticas / citologiaCélulas Dendríticas / metabolismoExossomos / metabolismoSangue Fetal / citologiaSangue Fetal / metabolismoHumanosEspectrometria de MassasMicroscopia ImunoeletrônicaNanotecnologiaProteômica / métodosReceptores da Transferrina / metabolismoReticulócitos / citologiaReticulócitos / metabolismoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83085https://www.arca.fiocruz.br/bitstream/icict/36904/1/license.txt4194663c61da82c21746deb8d6361188MD51ORIGINALProteomics study of human cord blood reticulocyte-derived exosomes.pdfProteomics study of human cord blood reticulocyte-derived exosomes.pdfapplication/pdf3040043https://www.arca.fiocruz.br/bitstream/icict/36904/2/Proteomics%20study%20of%20human%20cord%20blood%20reticulocyte-derived%20exosomes.pdf782e300426c6467abece6619ad792a54MD52TEXTProteomics study of human cord blood reticulocyte-derived exosomes.pdf.txtProteomics study of human cord blood reticulocyte-derived exosomes.pdf.txtExtracted texttext/plain52658https://www.arca.fiocruz.br/bitstream/icict/36904/3/Proteomics%20study%20of%20human%20cord%20blood%20reticulocyte-derived%20exosomes.pdf.txte5ff97381a91aa550fe02bf1746ce9edMD53icict/369042019-12-16 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| dc.title.pt_BR.fl_str_mv |
Proteomics study of human cord blood reticulocyte-derived exosomes |
| title |
Proteomics study of human cord blood reticulocyte-derived exosomes |
| spellingShingle |
Proteomics study of human cord blood reticulocyte-derived exosomes Díaz-Varela, Míriam Proteomics Human cord blood Reticulocyte-derived exosomes Bancos de Sangue Técnicas de Cultura de Células Células Cultivadas Células Dendríticas / citologia Células Dendríticas / metabolismo Exossomos / metabolismo Sangue Fetal / citologia Sangue Fetal / metabolismo Humanos Espectrometria de Massas Microscopia Imunoeletrônica Nanotecnologia Proteômica / métodos Receptores da Transferrina / metabolismo Reticulócitos / citologia Reticulócitos / metabolismo |
| title_short |
Proteomics study of human cord blood reticulocyte-derived exosomes |
| title_full |
Proteomics study of human cord blood reticulocyte-derived exosomes |
| title_fullStr |
Proteomics study of human cord blood reticulocyte-derived exosomes |
| title_full_unstemmed |
Proteomics study of human cord blood reticulocyte-derived exosomes |
| title_sort |
Proteomics study of human cord blood reticulocyte-derived exosomes |
| author |
Díaz-Varela, Míriam |
| author_facet |
Díaz-Varela, Míriam Menezes-Neto, Armando de Perez-Zsolt, Daniel Gámez-Valero, Ana Seguí-Barber, Joan Izquierdo-Useros, Nuria Martinez-Picado, Javier Fernández-Becerra, Carmen Portillo, Hernando A. del |
| author_role |
author |
| author2 |
Menezes-Neto, Armando de Perez-Zsolt, Daniel Gámez-Valero, Ana Seguí-Barber, Joan Izquierdo-Useros, Nuria Martinez-Picado, Javier Fernández-Becerra, Carmen Portillo, Hernando A. del |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Díaz-Varela, Míriam Menezes-Neto, Armando de Perez-Zsolt, Daniel Gámez-Valero, Ana Seguí-Barber, Joan Izquierdo-Useros, Nuria Martinez-Picado, Javier Fernández-Becerra, Carmen Portillo, Hernando A. del |
| dc.subject.en.pt_BR.fl_str_mv |
Proteomics Human cord blood Reticulocyte-derived exosomes |
| topic |
Proteomics Human cord blood Reticulocyte-derived exosomes Bancos de Sangue Técnicas de Cultura de Células Células Cultivadas Células Dendríticas / citologia Células Dendríticas / metabolismo Exossomos / metabolismo Sangue Fetal / citologia Sangue Fetal / metabolismo Humanos Espectrometria de Massas Microscopia Imunoeletrônica Nanotecnologia Proteômica / métodos Receptores da Transferrina / metabolismo Reticulócitos / citologia Reticulócitos / metabolismo |
| dc.subject.decs.pt_BR.fl_str_mv |
Bancos de Sangue Técnicas de Cultura de Células Células Cultivadas Células Dendríticas / citologia Células Dendríticas / metabolismo Exossomos / metabolismo Sangue Fetal / citologia Sangue Fetal / metabolismo Humanos Espectrometria de Massas Microscopia Imunoeletrônica Nanotecnologia Proteômica / métodos Receptores da Transferrina / metabolismo Reticulócitos / citologia Reticulócitos / metabolismo |
| description |
Universidade de Barcelona. Hospital Clínic. ISGlobal. Barcelona, Espanha. |
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2018 |
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2018 |
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2019-11-06T15:09:17Z |
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2019-11-06T15:09:17Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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DÍAZ-VARELA, M. et al. Proteomics study of human cord blood reticulocyte-derived exosomes. Scientific Reports, v. 8, n. 1, p. 1-11, 2018. |
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https://www.arca.fiocruz.br/handle/icict/36904 |
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2045-2322 |
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10.1038/s41598-018-32386-2 |
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DÍAZ-VARELA, M. et al. Proteomics study of human cord blood reticulocyte-derived exosomes. Scientific Reports, v. 8, n. 1, p. 1-11, 2018. 2045-2322 10.1038/s41598-018-32386-2 |
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