Oral Tolerance Induced by Heat Shock Protein 65-Producing Lactococcus lactis Mitigates Inflammation in Leishmania braziliensis Infection
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/48040 |
Resumo: | CNPq grants numbers 428371/ 2018-3 and 304876/2019-4. PVG received a pos-doc fellowship from Funda̧cão Oswaldo Cruz, Programa Inova. VAA, TUM, AMCF, and CIB are senior investigators of CNPq. This study is financed in part by the Coordena̧cão de Aperfei̧coamento de Pessoal de Nıveĺ SUperior (CAPES)-Brazil, Finance Code 001. |
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Guerra, Priscila ValeraAndrade, Camila MattosNunes, Ivanéia ValerianoGama, Brena CardosoTibúrcio, RafaeldosSantos, Washington Luis ConradoAzevedo, Vasco AristonTavares, Natalia MachadoRebouças, Juliana de SouzaMaiolii, Tatiani UceliFaria, Ana Maria CaetanoBrodskyn, Cláudia Ida2021-07-07T17:23:59Z2021-07-07T17:23:59Z2021GUERRA, Priscila Valera et al. Oral Tolerance Induced by Heat Shock Protein 65-Producing Lactococcus lactis Mitigates Inflammation in Leishmania braziliensis Infection. Frontiers in Immunology, 2021.1664-3224https://www.arca.fiocruz.br/handle/icict/4804010.3389/fimmu.2021.647987CNPq grants numbers 428371/ 2018-3 and 304876/2019-4. PVG received a pos-doc fellowship from Funda̧cão Oswaldo Cruz, Programa Inova. VAA, TUM, AMCF, and CIB are senior investigators of CNPq. This study is financed in part by the Coordena̧cão de Aperfei̧coamento de Pessoal de Nıveĺ SUperior (CAPES)-Brazil, Finance Code 001.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório da Interação Parasita-Hospedeiro e Epidemiologia. Salvador, BA, Brasil / Centro Universitário Christus. Curso de Medicina, Fortaleza, CE, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório da Interação Parasita-Hospedeiro e Epidemiologia. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório da Interação Parasita-Hospedeiro e Epidemiologia. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório da Interação Parasita-Hospedeiro e Epidemiologia. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório da Interação Parasita-Hospedeiro e Epidemiologia. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Departamento de Patologia e Medicina Legal. Salvador, BA, Brasil.Universidade Federal de Minais Gerais. Instituto de Ciências Biomédicas. Departamento de Genética, Ecologia e Evolução. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório da Interação Parasita-Hospedeiro e Epidemiologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia. Instituto de Investigação em Imunologia. São Paulo, SP, Brasil.Universidade de Pernambuco. Instituto de Ciências Biológicas. Programa de Pós Graduação em Ciências da Saúde. Recife, PE, Brasil.Universidade Federal de Minas Gerais. Escola de Enfermagem. Departamento de Nutrição. Belo Horizonte, MG, Brasil.Instituto Nacional de Ciência e Tecnologia. Instituto de Investigação em Imunologia. São Paulo, SP, Brasil / Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório da Interação Parasita-Hospedeiro e Epidemiologia. Salvador, BA, Brasil / Universidade Federal de Minas Gerais. Escola de Enfermagem. Departamento de Nutrição. Belo Horizonte, MG, Brasil.Cutaneous leishmaniasis caused by L. braziliensis induces a pronounced Th1 inflammatory response characterized by IFN-g production. Even in the absence of parasites, lesions result from a severe inflammatory response in which inflammatory cytokines play an important role. Different approaches have been used to evaluate the therapeutic potential of orally administrated heat shock proteins (Hsp). These proteins are evolutionarily preserved from bacteria to humans, highly expressed under inflammatory conditions and described as immunodominant antigens. Tolerance induced by the oral administration of Hsp65 is capable of suppressing inflammation and inducing differentiation in regulatory cells, and has been successfully demonstrated in several experimental models of autoimmune and inflammatory diseases. We initially administered recombinant Lactococcus lactis (L. lactis) prior to infection as a proof of concept, in order to verify its immunomodulatory potential in the inflammatory response arising from L. braziliensis. Using this experimental approach, we demonstrated that the oral administration of a recombinant L. lactis strain, which produces and secretes Hsp65 from Mycobacterium leprae directly into the gut, mitigated the effects of inflammation caused by L. braziliensis infection in association or not with PAM 3CSK4 (N-a-PalmitoylS-[2,3-bis(palmitoyloxy)-(2RS)-propyl]-L-cysteine, a TLR2 agonist). This was evidenced by the production of anti-inflammatory cytokines and the expansion of regulatory T cells in the draining lymph nodes of BALB/c mice. Our in vitro experimental results suggest that IL-10, TLR-2 and LAP are important immunomodulators in L. braziliensis infection. In addition, recombinant L. lactis administered 4 weeks after infection was observed to decrease lesion size, as well as the number of parasites, and produced a higher IL-10 production and decrease IFN-g secretion. Together, these results indicate that Hsp65- producing L. lactis can be considered as an alternative candidate for treatment in both autoimmune diseases, as well as in chronic infections that cause inflammatory disease.engFrontiers MediaLeishmaniose Cutâneaproteína de choque térmicoLactococcus lactisTerapêuticaInterleucina-10InflamaçãoLeishmania braziliensisHeat shock protein 65Lactococcus lactisOral toleranceIL-10TLR2TGF-bOral Tolerance Induced by Heat Shock Protein 65-Producing Lactococcus lactis Mitigates Inflammation in Leishmania braziliensis Infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83097https://www.arca.fiocruz.br/bitstream/icict/48040/1/license.txt36b51ef91c52b5338d9d29ba0cc807bcMD51ORIGINALGuerra, Priscila Valera Oral Tolerance....pdfGuerra, Priscila 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| dc.title.pt_BR.fl_str_mv |
Oral Tolerance Induced by Heat Shock Protein 65-Producing Lactococcus lactis Mitigates Inflammation in Leishmania braziliensis Infection |
| title |
Oral Tolerance Induced by Heat Shock Protein 65-Producing Lactococcus lactis Mitigates Inflammation in Leishmania braziliensis Infection |
| spellingShingle |
Oral Tolerance Induced by Heat Shock Protein 65-Producing Lactococcus lactis Mitigates Inflammation in Leishmania braziliensis Infection Guerra, Priscila Valera Leishmaniose Cutânea proteína de choque térmico Lactococcus lactis Terapêutica Interleucina-10 Inflamação Leishmania braziliensis Heat shock protein 65 Lactococcus lactis Oral tolerance IL-10 TLR2 TGF-b |
| title_short |
Oral Tolerance Induced by Heat Shock Protein 65-Producing Lactococcus lactis Mitigates Inflammation in Leishmania braziliensis Infection |
| title_full |
Oral Tolerance Induced by Heat Shock Protein 65-Producing Lactococcus lactis Mitigates Inflammation in Leishmania braziliensis Infection |
| title_fullStr |
Oral Tolerance Induced by Heat Shock Protein 65-Producing Lactococcus lactis Mitigates Inflammation in Leishmania braziliensis Infection |
| title_full_unstemmed |
Oral Tolerance Induced by Heat Shock Protein 65-Producing Lactococcus lactis Mitigates Inflammation in Leishmania braziliensis Infection |
| title_sort |
Oral Tolerance Induced by Heat Shock Protein 65-Producing Lactococcus lactis Mitigates Inflammation in Leishmania braziliensis Infection |
| author |
Guerra, Priscila Valera |
| author_facet |
Guerra, Priscila Valera Andrade, Camila Mattos Nunes, Ivanéia Valeriano Gama, Brena Cardoso Tibúrcio, Rafael dosSantos, Washington Luis Conrado Azevedo, Vasco Ariston Tavares, Natalia Machado Rebouças, Juliana de Souza Maiolii, Tatiani Uceli Faria, Ana Maria Caetano Brodskyn, Cláudia Ida |
| author_role |
author |
| author2 |
Andrade, Camila Mattos Nunes, Ivanéia Valeriano Gama, Brena Cardoso Tibúrcio, Rafael dosSantos, Washington Luis Conrado Azevedo, Vasco Ariston Tavares, Natalia Machado Rebouças, Juliana de Souza Maiolii, Tatiani Uceli Faria, Ana Maria Caetano Brodskyn, Cláudia Ida |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Guerra, Priscila Valera Andrade, Camila Mattos Nunes, Ivanéia Valeriano Gama, Brena Cardoso Tibúrcio, Rafael dosSantos, Washington Luis Conrado Azevedo, Vasco Ariston Tavares, Natalia Machado Rebouças, Juliana de Souza Maiolii, Tatiani Uceli Faria, Ana Maria Caetano Brodskyn, Cláudia Ida |
| dc.subject.other.pt_BR.fl_str_mv |
Leishmaniose Cutânea proteína de choque térmico Lactococcus lactis Terapêutica Interleucina-10 Inflamação |
| topic |
Leishmaniose Cutânea proteína de choque térmico Lactococcus lactis Terapêutica Interleucina-10 Inflamação Leishmania braziliensis Heat shock protein 65 Lactococcus lactis Oral tolerance IL-10 TLR2 TGF-b |
| dc.subject.en.pt_BR.fl_str_mv |
Leishmania braziliensis Heat shock protein 65 Lactococcus lactis Oral tolerance IL-10 TLR2 TGF-b |
| description |
CNPq grants numbers 428371/ 2018-3 and 304876/2019-4. PVG received a pos-doc fellowship from Funda̧cão Oswaldo Cruz, Programa Inova. VAA, TUM, AMCF, and CIB are senior investigators of CNPq. This study is financed in part by the Coordena̧cão de Aperfei̧coamento de Pessoal de Nıveĺ SUperior (CAPES)-Brazil, Finance Code 001. |
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2021 |
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2021-07-07T17:23:59Z |
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2021-07-07T17:23:59Z |
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2021 |
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info:eu-repo/semantics/article |
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GUERRA, Priscila Valera et al. Oral Tolerance Induced by Heat Shock Protein 65-Producing Lactococcus lactis Mitigates Inflammation in Leishmania braziliensis Infection. Frontiers in Immunology, 2021. |
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https://www.arca.fiocruz.br/handle/icict/48040 |
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1664-3224 |
| dc.identifier.doi.none.fl_str_mv |
10.3389/fimmu.2021.647987 |
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GUERRA, Priscila Valera et al. Oral Tolerance Induced by Heat Shock Protein 65-Producing Lactococcus lactis Mitigates Inflammation in Leishmania braziliensis Infection. Frontiers in Immunology, 2021. 1664-3224 10.3389/fimmu.2021.647987 |
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