Phylogeography of the Lutzomyia gomezi (Diptera: Phlebotominae) on the Panama Isthmus

Detalhes bibliográficos
Autor(a) principal: Valderrama, Anayansi
Data de Publicação: 2014
Outros Autores: Tavares, Mara Garcia, Andrade Filho, Jose Dilermando
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/9449
Resumo: Instituto Conmemorativo Gorgas de Estudios de la Salud. Department of Medical Entomology. Panama, Panama
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spelling Valderrama, AnayansiTavares, Mara GarciaAndrade Filho, Jose Dilermando2015-02-06T16:03:00Z2015-02-06T16:03:00Z2014VALDERRAMA, Anayansi; TAVARES, Mara Garcia; ANDRADE FILHO, Jose Dilermando. Phylogeography of the Lutzomyia gomezi (Diptera: Phlebotominae) on the Panama Isthmus. Parasit Vectors, v. 7, p. 9, 2014.1756-3305https://www.arca.fiocruz.br/handle/icict/944910.1186/1756-3305-7-9.engBioMed CentralPhylogeography of the Lutzomyia gomezi (Diptera: Phlebotominae) on the Panama Isthmusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto Conmemorativo Gorgas de Estudios de la Salud. Department of Medical Entomology. Panama, PanamaUniversidade Federal de Viçosa. Departamento de Biologia. Viçosa, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Centro de Referência Nacional e Internacional para Flebotomíneos/Coleção de Flebotomíneos. Belo Horizonte, MG, BrasilBACKGROUND: Lutzomyia gomezi (Nitzulescu, 1931) is one of the main Leishmania (Vianna) panamensis vectors in Panama, and despite its medical significance, there are no population genetic studies regarding this species. In this study, we used the sequences of the mitochondrial gene cytochrome b/start of NADH1 and the nuclear elongation gene α-1 in order to analyze genetic variation and phylogeographic structure of the Lu. gomezi populations. METHODS: A total of 86 Lu. gomezi individuals were captured in 38 locations where cutaneous leishmaniasis occurred. DNA was extracted with phenol/chloroform methods and amplification of genes was performed using PCR primers for mitochondrial and nuclear markers. RESULTS: We found a total of 37 and 26 haplotypes of mitochondrial and nuclear genes, high haplotype diversity (h) for all three populations were detected with both molecular markers. Nucleotide diversity (π) was estimated to be high for all three populations with the mitochondrial marker, which was opposite to the estimate with the nuclear marker. In the AMOVA Φst recorded moderate (mitochondrial) and small (nuclear) population structure with statistical significance among populations. The analysis of the fixation index (Fst) used to measure the differentiation of populations showed that with the exception of the population located in the region of Bocas del Toro, the other populations presented with minor genetic differentiation. The median-Joining network of the mitochondrial marker reveled three clusters and recorded four haplotypes exclusively of localities sampled from Western Panama, demonstrating strong divergence. We found demographic population expansion with Fu´s Fs neutrality test. In the analysis mismatch distribution was observed as a bimodal curve. CONCLUSION: Lu. gomezi is a species with higher genetic pool or variability and mild population structure, due to possible capacity migration and local adaptation to environmental changes or colonization potential. Thus, knowledge of the genetic population and evolutionary history is useful to understand the implications of different population genetic structures for cutaneous leishmaniasis epidemiologyLeishmania panamensisCutaneous leishmaniasisGenetic variability and Phylogeographicinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/9449/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINAL2014_050.pdf2014_050.pdfapplication/pdf863649https://www.arca.fiocruz.br/bitstream/icict/9449/2/2014_050.pdf37b6ee2953b8277d04007a8b09ce071cMD52TEXT2014_050.pdf.txt2014_050.pdf.txtExtracted texttext/plain55653https://www.arca.fiocruz.br/bitstream/icict/9449/3/2014_050.pdf.txtdf750989d34de2d42daad223f6414a7aMD53icict/94492019-06-19 10:07:35.121oai:www.arca.fiocruz.br:icict/9449TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkFvIGNvbmNvcmRhciBlIGFjZWl0YXIgZXN0YSBsaWNlbsOnYSB2b2PDqiAoYXV0b3Igb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzKToKCmEpIERlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zDrXRpY2EgZGUgY29weXJpZ2h0IGRhIGVkaXRvcmEgZG8gc2V1IGRvY3VtZW50by4KCmIpIERlY2xhcmEgcXVlIGNvbmhlY2UgZSBhY2VpdGEgYXMgRGlyZXRyaXplcyBwYXJhIG8gUmVwb3NpdMOzcmlvIEluc3RpdHVjaW9uYWwgZGEgRnVuZGHDp8OjbyBPc3dhbGRvIENydXogKEZJT0NSVVopLgoKYykgQ29uY2VkZSDDoCBGSU9DUlVaIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSBhcnF1aXZhciwgcmVwcm9kdXppciwgY29udmVydGVyIChjb21vIGRlZmluaWRvIGEgc2VndWlyKSwgY29tdW5pY2FyCiAKZS9vdSBkaXN0cmlidWlyIG5vIFJlcG9zaXTDs3JpbyBkYSBGSU9DUlVaLCBvIGRvY3VtZW50byBlbnRyZWd1ZSAoaW5jbHVpbmRvIG8gcmVzdW1vL2Fic3RyYWN0KSBlbSBmb3JtYXRvIGRpZ2l0YWwgb3UgCgpwb3IgcXVhbHF1ZXIgb3V0cm8gbWVpby4KCmQpIERlY2xhcmEgcXVlIGF1dG9yaXphIGEgRklPQ1JVWiBhIGFycXVpdmFyIG1haXMgZGUgdW1hIGPDs3BpYSBkZXN0ZSBkb2N1bWVudG8gZSBjb252ZXJ0w6otbG8sIHNlbSBhbHRlcmFyIG8gc2V1IGNvbnRlw7pkbywgCgpwYXJhIHF1YWxxdWVyIGZvcm1hdG8gZGUgYXJxdWl2bywgbWVpbyBvdSBzdXBvcnRlLCBwYXJhIGVmZWl0b3MgZGUgc2VndXJhbsOnYSwgcHJlc2VydmHDp8OjbyAoYmFja3VwKSBlIGFjZXNzby4KCmUpIERlY2xhcmEgcXVlIG8gZG9jdW1lbnRvIHN1Ym1ldGlkbyDDqSBvIHNldSB0cmFiYWxobyBvcmlnaW5hbCwgZSBxdWUgZGV0w6ltIG8gZGlyZWl0byBkZSBjb25jZWRlciBhIHRlcmNlaXJvcyBvcyBkaXJlaXRvcyAKCmNvbnRpZG9zIG5lc3RhIGxpY2Vuw6dhLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGEgZW50cmVnYSBkbyBkb2N1bWVudG8gbsOjbyBpbmZyaW5nZSBvcyBkaXJlaXRvcyBkZSBxdWFscXVlciBvdXRyYSBwZXNzb2Egb3UgZW50aWRhZGUuCgpmKSBEZWNsYXJhIHF1ZSwgbm8gY2FzbyBkbyBkb2N1bWVudG8gc3VibWV0aWRvIGNvbnRlciBtYXRlcmlhbCBkbyBxdWFsIG7Do28gZGV0w6ltIG9zIGRpcmVpdG9zIGRlIGF1dG9yLCBvYnRldmUgYSBhdXRvcml6YcOnw6NvIAoKaXJyZXN0cml0YSBkbyByZXNwZWN0aXZvIGRldGVudG9yIGRlc3NlcyBkaXJlaXRvcywgcGFyYSBjZWRlciBhIEZJT0NSVVogb3MgZGlyZWl0b3MgcmVxdWVyaWRvcyBwb3IgZXN0YSBMaWNlbsOnYSBlIGF1dG9yaXphciBhIAoKdXRpbGl6w6EtbG9zIGxlZ2FsbWVudGUuIERlY2xhcmEgdGFtYsOpbSBxdWUgZXNzZSBtYXRlcmlhbCBjdWpvcyBkaXJlaXRvcyBzw6NvIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIGlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIAoKbm8gdGV4dG8gb3UgY29udGXDumRvIGRvIGRvY3VtZW50byBlbnRyZWd1ZS4KCmcpIFNFIE8gRE9DVU1FTlRPIEVOVFJFR1VFIMOJIEJBU0VBRE8gRU0gVFJBQkFMSE8gRklOQU5DSUFETyBPVSBBUE9JQURPIFBPUiBPVVRSQSBJTlNUSVRVScOHw4NPIFFVRSBOw4NPIEEgRklPQ1JVWiwgREVDTEFSQSBRVUUgQ1VNUFJJVSAKClFVQUlTUVVFUiBPQlJJR0HDh8OVRVMgRVhJR0lEQVMgUEVMTyBSRVNQRUNUSVZPIENPTlRSQVRPIE9VIEFDT1JETy4gQSBGSU9DUlVaIGlkZW50aWZpY2Fyw6EgY2xhcmFtZW50ZSBvKHMpIG5vbWUocykgZG8ocykgYXV0b3IoZXMpIGRvcyAKCmRpcmVpdG9zIGRvIGRvY3VtZW50byBlbnRyZWd1ZSBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIHBhcmEgYWzDqW0gZG8gcHJldmlzdG8gbmEgYWzDrW5lYSBjKS4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-06-19T13:07:35Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Phylogeography of the Lutzomyia gomezi (Diptera: Phlebotominae) on the Panama Isthmus
title Phylogeography of the Lutzomyia gomezi (Diptera: Phlebotominae) on the Panama Isthmus
spellingShingle Phylogeography of the Lutzomyia gomezi (Diptera: Phlebotominae) on the Panama Isthmus
Valderrama, Anayansi
Leishmania panamensis
Cutaneous leishmaniasis
Genetic variability and Phylogeographic
title_short Phylogeography of the Lutzomyia gomezi (Diptera: Phlebotominae) on the Panama Isthmus
title_full Phylogeography of the Lutzomyia gomezi (Diptera: Phlebotominae) on the Panama Isthmus
title_fullStr Phylogeography of the Lutzomyia gomezi (Diptera: Phlebotominae) on the Panama Isthmus
title_full_unstemmed Phylogeography of the Lutzomyia gomezi (Diptera: Phlebotominae) on the Panama Isthmus
title_sort Phylogeography of the Lutzomyia gomezi (Diptera: Phlebotominae) on the Panama Isthmus
author Valderrama, Anayansi
author_facet Valderrama, Anayansi
Tavares, Mara Garcia
Andrade Filho, Jose Dilermando
author_role author
author2 Tavares, Mara Garcia
Andrade Filho, Jose Dilermando
author2_role author
author
dc.contributor.author.fl_str_mv Valderrama, Anayansi
Tavares, Mara Garcia
Andrade Filho, Jose Dilermando
dc.subject.en.pt_BR.fl_str_mv Leishmania panamensis
Cutaneous leishmaniasis
Genetic variability and Phylogeographic
topic Leishmania panamensis
Cutaneous leishmaniasis
Genetic variability and Phylogeographic
description Instituto Conmemorativo Gorgas de Estudios de la Salud. Department of Medical Entomology. Panama, Panama
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-02-06T16:03:00Z
dc.date.available.fl_str_mv 2015-02-06T16:03:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv VALDERRAMA, Anayansi; TAVARES, Mara Garcia; ANDRADE FILHO, Jose Dilermando. Phylogeography of the Lutzomyia gomezi (Diptera: Phlebotominae) on the Panama Isthmus. Parasit Vectors, v. 7, p. 9, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/9449
dc.identifier.issn.none.fl_str_mv 1756-3305
dc.identifier.doi.none.fl_str_mv 10.1186/1756-3305-7-9.
identifier_str_mv VALDERRAMA, Anayansi; TAVARES, Mara Garcia; ANDRADE FILHO, Jose Dilermando. Phylogeography of the Lutzomyia gomezi (Diptera: Phlebotominae) on the Panama Isthmus. Parasit Vectors, v. 7, p. 9, 2014.
1756-3305
10.1186/1756-3305-7-9.
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dc.publisher.none.fl_str_mv BioMed Central
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