Molecular characterization of canine filarioids in a previously non‑endemic area of Rio de Janeiro State, Brazil
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.1007/s00436-022-07433-7 |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/51758 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil. |
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Vieira, Viviane Marques de AndradeMartiniano, Nicole Oliveira MouraSilva, Priscila Pinho daPaulino, Érica TexFernandes, Priscila do AmaralLabarthe, NormaGazêta, Gilberto SallesMoraes Neto, Antonio Henrique Almeida de2022-03-21T12:02:13Z2022-03-21T12:02:13Z2022VIEIRA, Viviane Marques de Andrade et al. Molecular characterization of canine filarioids in a previously non‑endemic area of Rio de Janeiro State, Brazil. Parasitology Research, On line Jan. 2022, p. 1 - 8, 2022.2090-0031https://www.arca.fiocruz.br/handle/icict/5175810.1007/s00436-022-07433-7engSpringerDirofilaria immitisAcanthocheilonema reconditumDirofilarioseZoonose parasitária12S rDNADiagnóstico molecularDirofilaria immitisAcanthocheilonema reconditumHeartworm diseaseParasitic zoonosis12S rDNAMolecular diagnosisMolecular characterization of canine filarioids in a previously non‑endemic area of Rio de Janeiro State, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Referência Nacional em Vetores das Riquettsioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil.Laborlife Análises Clínicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Programa de Pós‑Graduação em Bioética, Ética Aplicada e Saúde Coletiva. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Referência Nacional em Vetores das Riquettsioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil.Dirofilaria immitis is the causative agent of canine heartworm disease, a severe health problem in dogs, especially in coastal areas of tropical and subtropical regions of the world. We employed molecular methods to investigate the occurrence of canine infection by filarioids in five municipalities of Baixada Fluminense (Magé, Duque de Caxias, Guapimirim, Nova Iguaçu, and São João de Meriti), a non-endemic area of Rio de Janeiro State, Southeast Brazil. A total of 110 canine blood samples collected from 2017 to 2018 and positive for microfilariae at the modified Knott’s test were screened by cPCR targeting DNA fragments of the 12S rDNA gene for filarial nematodes. Seventy-seven samples (70%) tested positive at the molecular analysis. Of these, 72 were identified as D. immitis and 5 (4.5%) as Acanthocheilonema reconditum. Dirofilaria repens was not detected in the studied municipalities of Baixada Fluminense. This is the first record of D. immitis and A. reconditum in the Baixada Fluminense region, Rio de Janeiro State, Brazil. The prevalence of D. immitis cases in the five municipalities suggests the establishment and maintenance of its enzootic cycle in the studied region, which indicate vulnerability to the occurrence of epidemic cycles and, possibly, human cases.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/51758/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALGilberto_Gazeta_etal_IOC_2022.pdfGilberto_Gazeta_etal_IOC_2022.pdfapplication/pdf1267208https://www.arca.fiocruz.br/bitstream/icict/51758/2/Gilberto_Gazeta_etal_IOC_2022.pdf7599c09d8340379d26337ad411e908b5MD52icict/517582022-03-21 09:02:13.702oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-03-21T12:02:13Repositório Institucional da FIOCRUZ (ARCA) - 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| dc.title.pt_BR.fl_str_mv |
Molecular characterization of canine filarioids in a previously non‑endemic area of Rio de Janeiro State, Brazil |
| title |
Molecular characterization of canine filarioids in a previously non‑endemic area of Rio de Janeiro State, Brazil |
| spellingShingle |
Molecular characterization of canine filarioids in a previously non‑endemic area of Rio de Janeiro State, Brazil Vieira, Viviane Marques de Andrade Dirofilaria immitis Acanthocheilonema reconditum Dirofilariose Zoonose parasitária 12S rDNA Diagnóstico molecular Dirofilaria immitis Acanthocheilonema reconditum Heartworm disease Parasitic zoonosis 12S rDNA Molecular diagnosis |
| title_short |
Molecular characterization of canine filarioids in a previously non‑endemic area of Rio de Janeiro State, Brazil |
| title_full |
Molecular characterization of canine filarioids in a previously non‑endemic area of Rio de Janeiro State, Brazil |
| title_fullStr |
Molecular characterization of canine filarioids in a previously non‑endemic area of Rio de Janeiro State, Brazil |
| title_full_unstemmed |
Molecular characterization of canine filarioids in a previously non‑endemic area of Rio de Janeiro State, Brazil |
| title_sort |
Molecular characterization of canine filarioids in a previously non‑endemic area of Rio de Janeiro State, Brazil |
| author |
Vieira, Viviane Marques de Andrade |
| author_facet |
Vieira, Viviane Marques de Andrade Martiniano, Nicole Oliveira Moura Silva, Priscila Pinho da Paulino, Érica Tex Fernandes, Priscila do Amaral Labarthe, Norma Gazêta, Gilberto Salles Moraes Neto, Antonio Henrique Almeida de |
| author_role |
author |
| author2 |
Martiniano, Nicole Oliveira Moura Silva, Priscila Pinho da Paulino, Érica Tex Fernandes, Priscila do Amaral Labarthe, Norma Gazêta, Gilberto Salles Moraes Neto, Antonio Henrique Almeida de |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Vieira, Viviane Marques de Andrade Martiniano, Nicole Oliveira Moura Silva, Priscila Pinho da Paulino, Érica Tex Fernandes, Priscila do Amaral Labarthe, Norma Gazêta, Gilberto Salles Moraes Neto, Antonio Henrique Almeida de |
| dc.subject.other.pt_BR.fl_str_mv |
Dirofilaria immitis Acanthocheilonema reconditum Dirofilariose Zoonose parasitária 12S rDNA Diagnóstico molecular |
| topic |
Dirofilaria immitis Acanthocheilonema reconditum Dirofilariose Zoonose parasitária 12S rDNA Diagnóstico molecular Dirofilaria immitis Acanthocheilonema reconditum Heartworm disease Parasitic zoonosis 12S rDNA Molecular diagnosis |
| dc.subject.en.pt_BR.fl_str_mv |
Dirofilaria immitis Acanthocheilonema reconditum Heartworm disease Parasitic zoonosis 12S rDNA Molecular diagnosis |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil. |
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2022 |
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2022-03-21T12:02:13Z |
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2022-03-21T12:02:13Z |
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2022 |
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info:eu-repo/semantics/article |
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VIEIRA, Viviane Marques de Andrade et al. Molecular characterization of canine filarioids in a previously non‑endemic area of Rio de Janeiro State, Brazil. Parasitology Research, On line Jan. 2022, p. 1 - 8, 2022. |
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https://www.arca.fiocruz.br/handle/icict/51758 |
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2090-0031 |
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10.1007/s00436-022-07433-7 |
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VIEIRA, Viviane Marques de Andrade et al. Molecular characterization of canine filarioids in a previously non‑endemic area of Rio de Janeiro State, Brazil. Parasitology Research, On line Jan. 2022, p. 1 - 8, 2022. 2090-0031 10.1007/s00436-022-07433-7 |
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