Molecular characterization of canine filarioids in a previously non‑endemic area of Rio de Janeiro State, Brazil

Detalhes bibliográficos
Autor(a) principal: Vieira, Viviane Marques de Andrade
Data de Publicação: 2022
Outros Autores: Martiniano, Nicole Oliveira Moura, Silva, Priscila Pinho da, Paulino, Érica Tex, Fernandes, Priscila do Amaral, Labarthe, Norma, Gazêta, Gilberto Salles, Moraes Neto, Antonio Henrique Almeida de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/51758
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil.
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spelling Vieira, Viviane Marques de AndradeMartiniano, Nicole Oliveira MouraSilva, Priscila Pinho daPaulino, Érica TexFernandes, Priscila do AmaralLabarthe, NormaGazêta, Gilberto SallesMoraes Neto, Antonio Henrique Almeida de2022-03-21T12:02:13Z2022-03-21T12:02:13Z2022VIEIRA, Viviane Marques de Andrade et al. Molecular characterization of canine filarioids in a previously non‑endemic area of Rio de Janeiro State, Brazil. Parasitology Research, On line Jan. 2022, p. 1 - 8, 2022.2090-0031https://www.arca.fiocruz.br/handle/icict/5175810.1007/s00436-022-07433-7engSpringerDirofilaria immitisAcanthocheilonema reconditumDirofilarioseZoonose parasitária12S rDNADiagnóstico molecularDirofilaria immitisAcanthocheilonema reconditumHeartworm diseaseParasitic zoonosis12S rDNAMolecular diagnosisMolecular characterization of canine filarioids in a previously non‑endemic area of Rio de Janeiro State, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Referência Nacional em Vetores das Riquettsioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil.Laborlife Análises Clínicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Programa de Pós‑Graduação em Bioética, Ética Aplicada e Saúde Coletiva. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Referência Nacional em Vetores das Riquettsioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil.Dirofilaria immitis is the causative agent of canine heartworm disease, a severe health problem in dogs, especially in coastal areas of tropical and subtropical regions of the world. We employed molecular methods to investigate the occurrence of canine infection by filarioids in five municipalities of Baixada Fluminense (Magé, Duque de Caxias, Guapimirim, Nova Iguaçu, and São João de Meriti), a non-endemic area of Rio de Janeiro State, Southeast Brazil. A total of 110 canine blood samples collected from 2017 to 2018 and positive for microfilariae at the modified Knott’s test were screened by cPCR targeting DNA fragments of the 12S rDNA gene for filarial nematodes. Seventy-seven samples (70%) tested positive at the molecular analysis. Of these, 72 were identified as D. immitis and 5 (4.5%) as Acanthocheilonema reconditum. Dirofilaria repens was not detected in the studied municipalities of Baixada Fluminense. This is the first record of D. immitis and A. reconditum in the Baixada Fluminense region, Rio de Janeiro State, Brazil. The prevalence of D. immitis cases in the five municipalities suggests the establishment and maintenance of its enzootic cycle in the studied region, which indicate vulnerability to the occurrence of epidemic cycles and, possibly, human cases.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/51758/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALGilberto_Gazeta_etal_IOC_2022.pdfGilberto_Gazeta_etal_IOC_2022.pdfapplication/pdf1267208https://www.arca.fiocruz.br/bitstream/icict/51758/2/Gilberto_Gazeta_etal_IOC_2022.pdf7599c09d8340379d26337ad411e908b5MD52icict/517582022-03-21 09:02:13.702oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-03-21T12:02:13Repositório Institucional da FIOCRUZ (ARCA) - 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dc.title.pt_BR.fl_str_mv Molecular characterization of canine filarioids in a previously non‑endemic area of Rio de Janeiro State, Brazil
title Molecular characterization of canine filarioids in a previously non‑endemic area of Rio de Janeiro State, Brazil
spellingShingle Molecular characterization of canine filarioids in a previously non‑endemic area of Rio de Janeiro State, Brazil
Vieira, Viviane Marques de Andrade
Dirofilaria immitis
Acanthocheilonema reconditum
Dirofilariose
Zoonose parasitária
12S rDNA
Diagnóstico molecular
Dirofilaria immitis
Acanthocheilonema reconditum
Heartworm disease
Parasitic zoonosis
12S rDNA
Molecular diagnosis
title_short Molecular characterization of canine filarioids in a previously non‑endemic area of Rio de Janeiro State, Brazil
title_full Molecular characterization of canine filarioids in a previously non‑endemic area of Rio de Janeiro State, Brazil
title_fullStr Molecular characterization of canine filarioids in a previously non‑endemic area of Rio de Janeiro State, Brazil
title_full_unstemmed Molecular characterization of canine filarioids in a previously non‑endemic area of Rio de Janeiro State, Brazil
title_sort Molecular characterization of canine filarioids in a previously non‑endemic area of Rio de Janeiro State, Brazil
author Vieira, Viviane Marques de Andrade
author_facet Vieira, Viviane Marques de Andrade
Martiniano, Nicole Oliveira Moura
Silva, Priscila Pinho da
Paulino, Érica Tex
Fernandes, Priscila do Amaral
Labarthe, Norma
Gazêta, Gilberto Salles
Moraes Neto, Antonio Henrique Almeida de
author_role author
author2 Martiniano, Nicole Oliveira Moura
Silva, Priscila Pinho da
Paulino, Érica Tex
Fernandes, Priscila do Amaral
Labarthe, Norma
Gazêta, Gilberto Salles
Moraes Neto, Antonio Henrique Almeida de
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Vieira, Viviane Marques de Andrade
Martiniano, Nicole Oliveira Moura
Silva, Priscila Pinho da
Paulino, Érica Tex
Fernandes, Priscila do Amaral
Labarthe, Norma
Gazêta, Gilberto Salles
Moraes Neto, Antonio Henrique Almeida de
dc.subject.other.pt_BR.fl_str_mv Dirofilaria immitis
Acanthocheilonema reconditum
Dirofilariose
Zoonose parasitária
12S rDNA
Diagnóstico molecular
topic Dirofilaria immitis
Acanthocheilonema reconditum
Dirofilariose
Zoonose parasitária
12S rDNA
Diagnóstico molecular
Dirofilaria immitis
Acanthocheilonema reconditum
Heartworm disease
Parasitic zoonosis
12S rDNA
Molecular diagnosis
dc.subject.en.pt_BR.fl_str_mv Dirofilaria immitis
Acanthocheilonema reconditum
Heartworm disease
Parasitic zoonosis
12S rDNA
Molecular diagnosis
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-03-21T12:02:13Z
dc.date.available.fl_str_mv 2022-03-21T12:02:13Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv VIEIRA, Viviane Marques de Andrade et al. Molecular characterization of canine filarioids in a previously non‑endemic area of Rio de Janeiro State, Brazil. Parasitology Research, On line Jan. 2022, p. 1 - 8, 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/51758
dc.identifier.issn.pt_BR.fl_str_mv 2090-0031
dc.identifier.doi.none.fl_str_mv 10.1007/s00436-022-07433-7
identifier_str_mv VIEIRA, Viviane Marques de Andrade et al. Molecular characterization of canine filarioids in a previously non‑endemic area of Rio de Janeiro State, Brazil. Parasitology Research, On line Jan. 2022, p. 1 - 8, 2022.
2090-0031
10.1007/s00436-022-07433-7
url https://www.arca.fiocruz.br/handle/icict/51758
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/51758/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/51758/2/Gilberto_Gazeta_etal_IOC_2022.pdf
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repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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