Participation of cytokines in the necrotic-inflammatory lesions in the heart and skeletal muscles of Calomys callosus infected with Trypanosoma cruzi
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2005 |
| Outros Autores: | |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/2653 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil |
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Santos, Isis Fernandes MagalhãesAndrade, Sonia Gumes2011-05-11T20:47:14Z2011-05-11T20:47:14Z2005MAGALHÃES-SANTOS, I. F.; ANDRADE, S. G. Clinics in Sports Medicine. Memorias do Instituto Oswaldo Cruz, Rio de Janeiro, v.100, n. 5, p. 555-561. aug., 20050074-0276https://www.arca.fiocruz.br/handle/icict/2653engParticipation of cytokines in the necrotic-inflammatory lesions in the heart and skeletal muscles of Calomys callosus infected with Trypanosoma cruziinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilCalomys callosus, a sylvatic reservoir of Trypanosoma cruzi, when infected with the Colombian strain (Biodeme Type III, T. cruzi I ) develops necrotic-inflammatory lesions and intense early fibrogenesis in the heart and skeletal muscles, that spontaneously regress. Participation of pro-inflammatory and pro-fibrogenic cytokines, such as tumor necrosis factor-alpha (TNF-α), gamma interferon (IFN-γ) , and tumor growth factor-beta (TGF-β), in the pathogenesis of the lesions is herein studied. Eighty C. callosus weighing 20 to 30 g were used. Seventy of them were inoculated with the Colombian strain (105 blood forms) and 10 were maintained as intact non-infected controls. After infection, C. callosus were sacrificed at different time-points from 15 to 70 days. The heart and skeletal muscle were processed for histopathology and cryopreserved for immunohistochemistry. Early necrotic lesions of parasitized skeletal muscle and myocardium with intense inflammatory lesions were present. Search for the in situ presence of TNF-α and IFN-γ, was performed using rat-IgG anti-mouse antibodies against these cytokines. For the in situ search of TGF-β, rabbit IgG anti-mouse antibodies were used. Immunolabeling of the cytokines in tissues of infected C. callosus was successful. The cytokines TNF-α, IFN-γ , and TGF-β were detected in the cytoplasm of macrophages and in the necrotic material from 15 to 45 days post-infection, decreasing their intensity until complete disappearance by the 65th day, which correlated with subsiding histopathological lesions. These findings suggest the participation of these cytokines in the control of parasite multiplication, in the development of an early fibrogenesis and in the regression of fibrotic-inflammatory lesions observed in C. callosus.Trypanosoma cruziCalomys callosusCytokinesTumor necrosis factor-alphaGamma interferonTumor growth factor-betaDoença de ChagasCitocinasMúsculo EsqueléticoTrypanosoma cruziMiocárdioFator Transformador de Crescimento betaFator de Necrose Tumoral alfaInterferon gamainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALMagalhaes-Santos IF Participation of cytokines....pdfMagalhaes-Santos IF Participation of cytokines....pdfapplication/pdf19475430https://www.arca.fiocruz.br/bitstream/icict/2653/1/Magalhaes-Santos%20IF%20Participation%20of%20cytokines....pdf6f3e94c32f134a41c7cb8b4e40ea4e0eMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81648https://www.arca.fiocruz.br/bitstream/icict/2653/2/license.txte095249ac7cacefbfe39684dfe45e706MD52TEXTMagalhaes-Santos IF Participation of cytokines....pdf.txtMagalhaes-Santos IF Participation of cytokines....pdf.txtExtracted texttext/plain32414https://www.arca.fiocruz.br/bitstream/icict/2653/5/Magalhaes-Santos%20IF%20Participation%20of%20cytokines....pdf.txtc21f0e259c8f329ae44682ba14838c4eMD55THUMBNAILMagalhaes-Santos IF Participation of cytokines....pdf.jpgMagalhaes-Santos IF Participation of cytokines....pdf.jpgGenerated Thumbnailimage/jpeg2310https://www.arca.fiocruz.br/bitstream/icict/2653/4/Magalhaes-Santos%20IF%20Participation%20of%20cytokines....pdf.jpg8b4fadaecb66207305c1ca7e2affa888MD54icict/26532023-03-15 14:32:59.764oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:32:59Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Participation of cytokines in the necrotic-inflammatory lesions in the heart and skeletal muscles of Calomys callosus infected with Trypanosoma cruzi |
| title |
Participation of cytokines in the necrotic-inflammatory lesions in the heart and skeletal muscles of Calomys callosus infected with Trypanosoma cruzi |
| spellingShingle |
Participation of cytokines in the necrotic-inflammatory lesions in the heart and skeletal muscles of Calomys callosus infected with Trypanosoma cruzi Santos, Isis Fernandes Magalhães Trypanosoma cruzi Calomys callosus Cytokines Tumor necrosis factor-alpha Gamma interferon Tumor growth factor-beta Doença de Chagas Citocinas Músculo Esquelético Trypanosoma cruzi Miocárdio Fator Transformador de Crescimento beta Fator de Necrose Tumoral alfa Interferon gama |
| title_short |
Participation of cytokines in the necrotic-inflammatory lesions in the heart and skeletal muscles of Calomys callosus infected with Trypanosoma cruzi |
| title_full |
Participation of cytokines in the necrotic-inflammatory lesions in the heart and skeletal muscles of Calomys callosus infected with Trypanosoma cruzi |
| title_fullStr |
Participation of cytokines in the necrotic-inflammatory lesions in the heart and skeletal muscles of Calomys callosus infected with Trypanosoma cruzi |
| title_full_unstemmed |
Participation of cytokines in the necrotic-inflammatory lesions in the heart and skeletal muscles of Calomys callosus infected with Trypanosoma cruzi |
| title_sort |
Participation of cytokines in the necrotic-inflammatory lesions in the heart and skeletal muscles of Calomys callosus infected with Trypanosoma cruzi |
| author |
Santos, Isis Fernandes Magalhães |
| author_facet |
Santos, Isis Fernandes Magalhães Andrade, Sonia Gumes |
| author_role |
author |
| author2 |
Andrade, Sonia Gumes |
| author2_role |
author |
| dc.contributor.author.fl_str_mv |
Santos, Isis Fernandes Magalhães Andrade, Sonia Gumes |
| dc.subject.en.pt_BR.fl_str_mv |
Trypanosoma cruzi Calomys callosus Cytokines Tumor necrosis factor-alpha Gamma interferon Tumor growth factor-beta |
| topic |
Trypanosoma cruzi Calomys callosus Cytokines Tumor necrosis factor-alpha Gamma interferon Tumor growth factor-beta Doença de Chagas Citocinas Músculo Esquelético Trypanosoma cruzi Miocárdio Fator Transformador de Crescimento beta Fator de Necrose Tumoral alfa Interferon gama |
| dc.subject.decs.pt_BR.fl_str_mv |
Doença de Chagas Citocinas Músculo Esquelético Trypanosoma cruzi Miocárdio Fator Transformador de Crescimento beta Fator de Necrose Tumoral alfa Interferon gama |
| description |
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil |
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2005 |
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2005 |
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2011-05-11T20:47:14Z |
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2011-05-11T20:47:14Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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MAGALHÃES-SANTOS, I. F.; ANDRADE, S. G. Clinics in Sports Medicine. Memorias do Instituto Oswaldo Cruz, Rio de Janeiro, v.100, n. 5, p. 555-561. aug., 2005 |
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https://www.arca.fiocruz.br/handle/icict/2653 |
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0074-0276 |
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MAGALHÃES-SANTOS, I. F.; ANDRADE, S. G. Clinics in Sports Medicine. Memorias do Instituto Oswaldo Cruz, Rio de Janeiro, v.100, n. 5, p. 555-561. aug., 2005 0074-0276 |
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