Xylopine Induces Oxidative Stress and Causes G2/M Phase Arrest, Triggering Caspase-Mediated Apoptosis by p53-Independent Pathway in HCT116 Cells
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/26043 |
Resumo: | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB), and Fundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM). |
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Santos, Luciano de SouzaSilva, Valdenizia RodriguesMenezes, Leociley Rocha AlencarSoares, Milena Botelho PereiraCosta, Emmanoel VilaçaBezerra, Daniel Pereira2018-04-23T19:01:01Z2018-04-23T19:01:01Z2017SANTOS, L. S. et al. Xylopine Induces Oxidative Stress and Causes G2/M Phase Arrest, Triggering Caspase-Mediated Apoptosis by p53-Independent Pathway in HCT116 Cells. Oxidative Medicine and Cellular Longevity, ID 7126872, 2017.1942-0900https://www.arca.fiocruz.br/handle/icict/2604310.1155/2017/7126872Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB), and Fundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM).Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilFederal University of Sergipe. Department of Chemistry. São Cristóvão, SE, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Center of Biotechnology and Cell therapy. Salvador, BA, BrazilFederal University of Amazonas. Department of Chemistry. Manaus, AM, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilXylopine is an aporphine alkaloid that has cytotoxic activity to cancer cells. In this study, the underlying mechanism of xylopine cytotoxicity was assessed in human colon carcinoma HCT116 cells. Xylopine displayed potent cytotoxicity in different cancer cell lines in monolayer cultures and in a 3D model of cancer multicellular spheroids formed from HCT116 cells. Typical morphology of apoptosis, cell cycle arrest in the G2/M phase, increased internucleosomal DNA fragmentation, loss of the mitochondrial transmembrane potential, and increased phosphatidylserine externalization and caspase-3 activation were observed in xylopine-treated HCT116 cells. Moreover, pretreatment with a caspase-3 inhibitor (Z-DEVD-FMK), but not with a p53 inhibitor (cyclic pifithrin-α), reduced xylopine-induced apoptosis, indicating induction of caspase-mediated apoptosis by the p53-independent pathway. Treatment with xylopine also caused an increase in the production of reactive oxygen/nitrogen species (ROS/RNS), including hydrogen peroxide and nitric oxide, but not superoxide anion, and reduced glutathione levels were decreased in xylopine-treated HCT116 cells. Application of the antioxidant N-acetylcysteine reduced the ROS levels and xylopine-induced apoptosis, indicating activation of ROS-mediated apoptosis pathway. In conclusion, xylopine has potent cytotoxicity to different cancer cell lines and is able to induce oxidative stress and G2/M phase arrest, triggering caspase-mediated apoptosis by the p53-independent pathway in HCT116 cells.engHindawi Publishing CorporationEstresse oxidativoApoptoseCaspasesAlcalóidesCélulas HCT116CarcinomaSaúde públicaNeoplasia do colonOxidative StressApoptosisCaspaseAlkaloidHCT116 CellsCarcinomaPublic healthColon neoplasmXylopine Induces Oxidative Stress and Causes G2/M Phase Arrest, Triggering Caspase-Mediated Apoptosis by p53-Independent Pathway in HCT116 Cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/26043/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSantos LS Xylipine 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| dc.title.pt_BR.fl_str_mv |
Xylopine Induces Oxidative Stress and Causes G2/M Phase Arrest, Triggering Caspase-Mediated Apoptosis by p53-Independent Pathway in HCT116 Cells |
| title |
Xylopine Induces Oxidative Stress and Causes G2/M Phase Arrest, Triggering Caspase-Mediated Apoptosis by p53-Independent Pathway in HCT116 Cells |
| spellingShingle |
Xylopine Induces Oxidative Stress and Causes G2/M Phase Arrest, Triggering Caspase-Mediated Apoptosis by p53-Independent Pathway in HCT116 Cells Santos, Luciano de Souza Estresse oxidativo Apoptose Caspases Alcalóides Células HCT116 Carcinoma Saúde pública Neoplasia do colon Oxidative Stress Apoptosis Caspase Alkaloid HCT116 Cells Carcinoma Public health Colon neoplasm |
| title_short |
Xylopine Induces Oxidative Stress and Causes G2/M Phase Arrest, Triggering Caspase-Mediated Apoptosis by p53-Independent Pathway in HCT116 Cells |
| title_full |
Xylopine Induces Oxidative Stress and Causes G2/M Phase Arrest, Triggering Caspase-Mediated Apoptosis by p53-Independent Pathway in HCT116 Cells |
| title_fullStr |
Xylopine Induces Oxidative Stress and Causes G2/M Phase Arrest, Triggering Caspase-Mediated Apoptosis by p53-Independent Pathway in HCT116 Cells |
| title_full_unstemmed |
Xylopine Induces Oxidative Stress and Causes G2/M Phase Arrest, Triggering Caspase-Mediated Apoptosis by p53-Independent Pathway in HCT116 Cells |
| title_sort |
Xylopine Induces Oxidative Stress and Causes G2/M Phase Arrest, Triggering Caspase-Mediated Apoptosis by p53-Independent Pathway in HCT116 Cells |
| author |
Santos, Luciano de Souza |
| author_facet |
Santos, Luciano de Souza Silva, Valdenizia Rodrigues Menezes, Leociley Rocha Alencar Soares, Milena Botelho Pereira Costa, Emmanoel Vilaça Bezerra, Daniel Pereira |
| author_role |
author |
| author2 |
Silva, Valdenizia Rodrigues Menezes, Leociley Rocha Alencar Soares, Milena Botelho Pereira Costa, Emmanoel Vilaça Bezerra, Daniel Pereira |
| author2_role |
author author author author author |
| dc.contributor.author.fl_str_mv |
Santos, Luciano de Souza Silva, Valdenizia Rodrigues Menezes, Leociley Rocha Alencar Soares, Milena Botelho Pereira Costa, Emmanoel Vilaça Bezerra, Daniel Pereira |
| dc.subject.other.pt_BR.fl_str_mv |
Estresse oxidativo Apoptose Caspases Alcalóides Células HCT116 Carcinoma Saúde pública Neoplasia do colon |
| topic |
Estresse oxidativo Apoptose Caspases Alcalóides Células HCT116 Carcinoma Saúde pública Neoplasia do colon Oxidative Stress Apoptosis Caspase Alkaloid HCT116 Cells Carcinoma Public health Colon neoplasm |
| dc.subject.en.pt_BR.fl_str_mv |
Oxidative Stress Apoptosis Caspase Alkaloid HCT116 Cells Carcinoma Public health Colon neoplasm |
| description |
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB), and Fundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM). |
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2017 |
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2017 |
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2018-04-23T19:01:01Z |
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2018-04-23T19:01:01Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
SANTOS, L. S. et al. Xylopine Induces Oxidative Stress and Causes G2/M Phase Arrest, Triggering Caspase-Mediated Apoptosis by p53-Independent Pathway in HCT116 Cells. Oxidative Medicine and Cellular Longevity, ID 7126872, 2017. |
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https://www.arca.fiocruz.br/handle/icict/26043 |
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1942-0900 |
| dc.identifier.doi.none.fl_str_mv |
10.1155/2017/7126872 |
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SANTOS, L. S. et al. Xylopine Induces Oxidative Stress and Causes G2/M Phase Arrest, Triggering Caspase-Mediated Apoptosis by p53-Independent Pathway in HCT116 Cells. Oxidative Medicine and Cellular Longevity, ID 7126872, 2017. 1942-0900 10.1155/2017/7126872 |
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https://www.arca.fiocruz.br/handle/icict/26043 |
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eng |
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openAccess |
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Hindawi Publishing Corporation |
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Hindawi Publishing Corporation |
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