Neutrophil-derived microparticles induce myeloperoxidase-mediated damage of vascular endothelial cells

Detalhes bibliográficos
Autor(a) principal: Pitanga, Thassila Nogueira
Data de Publicação: 2014
Outros Autores: França, Luciana Souza de Aragão, Rocha, Viviane Costa Junqueira, Meirelles, Thayna, Borges, Valeria de Matos, Gonçalves, Marilda de Souza, Pontes-de-Carvalho, Lain Carlos, Dutra, Alberto Augusto Noronha, dosSantos, Washington Luis Conrado
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7855
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Health Science Institute. Federal University of Bahia. Salvador, BA, Brasil
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spelling Pitanga, Thassila NogueiraFrança, Luciana Souza de AragãoRocha, Viviane Costa JunqueiraMeirelles, ThaynaBorges, Valeria de MatosGonçalves, Marilda de SouzaPontes-de-Carvalho, Lain CarlosDutra, Alberto Augusto NoronhadosSantos, Washington Luis Conrado2014-06-30T16:51:01Z2014-06-30T16:51:01Z2014PITANGA, T. N. et al. Neutrophil-derived microparticles induce myeloperoxidase-mediated damage of vascular endothelial cells. BMC Cell Biology, v. 15, n. 1, p.1-9, 2014.1471-2121https://www.arca.fiocruz.br/handle/icict/785510.1186/1471-2121-15-21engBioMed CentralNeutrophil-derived microparticles induce myeloperoxidase-mediated damage of vascular endothelial cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Health Science Institute. Federal University of Bahia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilHeart Institute. Faculty of Medicine. USP, São Paulo, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / University College London. Gower Street, London, UKFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilBackground: Upon activation neutrophil releases microparticles - small plasma membrane vesicles that contain cell surface proteins and cytoplasmic matter, with biological activities. In this study we investigated the potential role of myeloperoxidase in the endothelial cell injury caused by neutrophil-derived microparticles. Results: Microparticles were produced by activating human neutrophils with a calcium ionophore and characterized by flow cytometry and transmission and scanning electron microscopy. Myeloperoxidase activity was measured by luminol-dependent chemiluminescence. Neutrophil microparticles-induced injuries and morphological alterations in human umbilical vein endothelial cells (HUVECs) were evaluated by microscopy and flow cytometry. Neutrophil microparticles were characterized as structures bounded by lipid bilayers and were less than 1 μm in diameter. The microparticles also expressed CD66b, CD62L and myeloperoxidase, which are all commonly expressed on the surface of neutrophils, as well as exposition of phosphatidylserine. The activity of the myeloperoxidase present on the microparticles was confirmed by hypochlorous acid detection. This compound is only catalyzed by myeloperoxidase in the presence of hydrogen peroxide and chloride ion. The addition of sodium azide or taurine inhibited and reduced enzymatic activity, respectively. Exposure of HUVEC to neutrophil microparticles induced a loss of cell membrane integrity and morphological changes. The addition of sodium azide or myeloperoxidase-specific inhibitor-I consistently reduced the injury to the endothelial cells. Taurine addition reduced HUVEC morphological changes. Conclusions: We have demonstrated the presence of active myeloperoxidase in neutrophil microparticles and that the microparticle-associated myeloperoxidase cause injury to endothelial cells. Hence, the microparticle-associated myeloperoxidase-hydrogen peroxide-chloride system may contribute to widespread endothelial cell damage in conditions of neutrophil activation as observed in vasculitis and sepsis.Neutrophil microparticlesEndothelial cell injuryMyeloperoxidaseOxidative stressinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALPitanga TN neutrophil....pdfPitanga TN neutrophil....pdfapplication/pdf2264719https://www.arca.fiocruz.br/bitstream/icict/7855/1/Pitanga%20TN%20neutrophil....pdfab98062c28dc4440692eca1a151d3729MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7855/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTPitanga TN neutrophil....pdf.txtPitanga TN neutrophil....pdf.txtExtracted texttext/plain41049https://www.arca.fiocruz.br/bitstream/icict/7855/3/Pitanga%20TN%20neutrophil....pdf.txt4a5f5fe3376416c528b364ff223b16c6MD53icict/78552023-03-15 14:33:14.885oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:14Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Neutrophil-derived microparticles induce myeloperoxidase-mediated damage of vascular endothelial cells
title Neutrophil-derived microparticles induce myeloperoxidase-mediated damage of vascular endothelial cells
spellingShingle Neutrophil-derived microparticles induce myeloperoxidase-mediated damage of vascular endothelial cells
Pitanga, Thassila Nogueira
Neutrophil microparticles
Endothelial cell injury
Myeloperoxidase
Oxidative stress
title_short Neutrophil-derived microparticles induce myeloperoxidase-mediated damage of vascular endothelial cells
title_full Neutrophil-derived microparticles induce myeloperoxidase-mediated damage of vascular endothelial cells
title_fullStr Neutrophil-derived microparticles induce myeloperoxidase-mediated damage of vascular endothelial cells
title_full_unstemmed Neutrophil-derived microparticles induce myeloperoxidase-mediated damage of vascular endothelial cells
title_sort Neutrophil-derived microparticles induce myeloperoxidase-mediated damage of vascular endothelial cells
author Pitanga, Thassila Nogueira
author_facet Pitanga, Thassila Nogueira
França, Luciana Souza de Aragão
Rocha, Viviane Costa Junqueira
Meirelles, Thayna
Borges, Valeria de Matos
Gonçalves, Marilda de Souza
Pontes-de-Carvalho, Lain Carlos
Dutra, Alberto Augusto Noronha
dosSantos, Washington Luis Conrado
author_role author
author2 França, Luciana Souza de Aragão
Rocha, Viviane Costa Junqueira
Meirelles, Thayna
Borges, Valeria de Matos
Gonçalves, Marilda de Souza
Pontes-de-Carvalho, Lain Carlos
Dutra, Alberto Augusto Noronha
dosSantos, Washington Luis Conrado
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pitanga, Thassila Nogueira
França, Luciana Souza de Aragão
Rocha, Viviane Costa Junqueira
Meirelles, Thayna
Borges, Valeria de Matos
Gonçalves, Marilda de Souza
Pontes-de-Carvalho, Lain Carlos
Dutra, Alberto Augusto Noronha
dosSantos, Washington Luis Conrado
dc.subject.en.pt_BR.fl_str_mv Neutrophil microparticles
Endothelial cell injury
Myeloperoxidase
Oxidative stress
topic Neutrophil microparticles
Endothelial cell injury
Myeloperoxidase
Oxidative stress
description Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Health Science Institute. Federal University of Bahia. Salvador, BA, Brasil
publishDate 2014
dc.date.accessioned.fl_str_mv 2014-06-30T16:51:01Z
dc.date.available.fl_str_mv 2014-06-30T16:51:01Z
dc.date.issued.fl_str_mv 2014
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv PITANGA, T. N. et al. Neutrophil-derived microparticles induce myeloperoxidase-mediated damage of vascular endothelial cells. BMC Cell Biology, v. 15, n. 1, p.1-9, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7855
dc.identifier.issn.none.fl_str_mv 1471-2121
dc.identifier.doi.none.fl_str_mv 10.1186/1471-2121-15-21
identifier_str_mv PITANGA, T. N. et al. Neutrophil-derived microparticles induce myeloperoxidase-mediated damage of vascular endothelial cells. BMC Cell Biology, v. 15, n. 1, p.1-9, 2014.
1471-2121
10.1186/1471-2121-15-21
url https://www.arca.fiocruz.br/handle/icict/7855
dc.language.iso.fl_str_mv eng
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
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