Identification and characterization of sex-linked proteins of Schistosoma mansoni

Detalhes bibliográficos
Autor(a) principal: Maldonado Jr, Arnaldo
Data de Publicação: 1991
Outros Autores: Simone, Giovanni de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1590/S0074-02761991000100006
Texto Completo: https://www.arca.fiocruz.br/handle/icict/43422
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Biologia. Rio de Janeiro, RJ, Brasil.
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spelling Maldonado Jr, ArnaldoSimone, Giovanni de2020-09-17T13:16:22Z2020-09-17T13:16:22Z1991MALDONADO JR., A.; SIMONE, Giovanni de. Identification and characterization of sex-linked proteins of Schistosoma mansoni. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 86, n. 1, p. 31-36, Jan./Mar. 1991.0074-0276https://www.arca.fiocruz.br/handle/icict/4342210.1590/S0074-027619910001000061678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Schistosoma mansoniIdentificaçãoClassificaçãoProteínas ligadas ao sexoSex-linked proteinsSchistosoma mansoniIdentificationClassificationIdentification and characterization of sex-linked proteins of Schistosoma mansoniinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Biologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.The proteins of adults worms (male and female) of two isolates (BH and RJ) of Shistosoma mansoni were extracted using Triton X-114 phase separation. The SDS-polyacrilamide gel electrophoresis profiles of the three phases (detergent, aqueous and insoluble proteins) obtained were compared after Coomassie blue and silver staining, surface radioiodination and Western blotting. No major differences were detected between the 2 isolates. Of the 25 or more proteins which partitioned into the detergent phase, only about 8 proteins could be surface radiodinated on live adult worms. A comparison was also made between the profiles of mael and females worms, isolated from bisexually infected mice. Two major female-specific and one male-specific band were detected by silver and/or Coomassie staining. The female bands, 32 KDa and 18 KDa, partitioned into the detergent and aqueous phase, respectively. The male-specific band of 42 KDa remained in the insoluble phase. 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dc.title.pt_BR.fl_str_mv Identification and characterization of sex-linked proteins of Schistosoma mansoni
title Identification and characterization of sex-linked proteins of Schistosoma mansoni
spellingShingle Identification and characterization of sex-linked proteins of Schistosoma mansoni
Maldonado Jr, Arnaldo
Schistosoma mansoni
Identificação
Classificação
Proteínas ligadas ao sexo
Sex-linked proteins
Schistosoma mansoni
Identification
Classification
title_short Identification and characterization of sex-linked proteins of Schistosoma mansoni
title_full Identification and characterization of sex-linked proteins of Schistosoma mansoni
title_fullStr Identification and characterization of sex-linked proteins of Schistosoma mansoni
title_full_unstemmed Identification and characterization of sex-linked proteins of Schistosoma mansoni
title_sort Identification and characterization of sex-linked proteins of Schistosoma mansoni
author Maldonado Jr, Arnaldo
author_facet Maldonado Jr, Arnaldo
Simone, Giovanni de
author_role author
author2 Simone, Giovanni de
author2_role author
dc.contributor.author.fl_str_mv Maldonado Jr, Arnaldo
Simone, Giovanni de
dc.subject.other.pt_BR.fl_str_mv Schistosoma mansoni
Identificação
Classificação
Proteínas ligadas ao sexo
topic Schistosoma mansoni
Identificação
Classificação
Proteínas ligadas ao sexo
Sex-linked proteins
Schistosoma mansoni
Identification
Classification
dc.subject.en.pt_BR.fl_str_mv Sex-linked proteins
Schistosoma mansoni
Identification
Classification
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Biologia. Rio de Janeiro, RJ, Brasil.
publishDate 1991
dc.date.issued.fl_str_mv 1991
dc.date.accessioned.fl_str_mv 2020-09-17T13:16:22Z
dc.date.available.fl_str_mv 2020-09-17T13:16:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv MALDONADO JR., A.; SIMONE, Giovanni de. Identification and characterization of sex-linked proteins of Schistosoma mansoni. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 86, n. 1, p. 31-36, Jan./Mar. 1991.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/43422
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02761991000100006
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv MALDONADO JR., A.; SIMONE, Giovanni de. Identification and characterization of sex-linked proteins of Schistosoma mansoni. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 86, n. 1, p. 31-36, Jan./Mar. 1991.
0074-0276
10.1590/S0074-02761991000100006
1678-8060
url https://www.arca.fiocruz.br/handle/icict/43422
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
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