Identification and characterization of sex-linked proteins of Schistosoma mansoni
Autor(a) principal: | |
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Data de Publicação: | 1991 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
DOI: | 10.1590/S0074-02761991000100006 |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/43422 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Biologia. Rio de Janeiro, RJ, Brasil. |
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Maldonado Jr, ArnaldoSimone, Giovanni de2020-09-17T13:16:22Z2020-09-17T13:16:22Z1991MALDONADO JR., A.; SIMONE, Giovanni de. Identification and characterization of sex-linked proteins of Schistosoma mansoni. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 86, n. 1, p. 31-36, Jan./Mar. 1991.0074-0276https://www.arca.fiocruz.br/handle/icict/4342210.1590/S0074-027619910001000061678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Schistosoma mansoniIdentificaçãoClassificaçãoProteínas ligadas ao sexoSex-linked proteinsSchistosoma mansoniIdentificationClassificationIdentification and characterization of sex-linked proteins of Schistosoma mansoniinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Biologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.The proteins of adults worms (male and female) of two isolates (BH and RJ) of Shistosoma mansoni were extracted using Triton X-114 phase separation. The SDS-polyacrilamide gel electrophoresis profiles of the three phases (detergent, aqueous and insoluble proteins) obtained were compared after Coomassie blue and silver staining, surface radioiodination and Western blotting. No major differences were detected between the 2 isolates. Of the 25 or more proteins which partitioned into the detergent phase, only about 8 proteins could be surface radiodinated on live adult worms. A comparison was also made between the profiles of mael and females worms, isolated from bisexually infected mice. Two major female-specific and one male-specific band were detected by silver and/or Coomassie staining. The female bands, 32 KDa and 18 KDa, partitioned into the detergent and aqueous phase, respectively. The male-specific band of 42 KDa remained in the insoluble phase. Antigenic differences between male and females protins were detected by Western vlotting using a sera from infected Nectomys squamipes.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/43422/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALAMALdonadoJR_SGSimone_IOC_1991.pdfAMALdonadoJR_SGSimone_IOC_1991.pdfapplication/pdf316298https://www.arca.fiocruz.br/bitstream/icict/43422/2/AMALdonadoJR_SGSimone_IOC_1991.pdf3c3df95f0a2deb382a6f6afe54033f45MD52TEXTAMALdonadoJR_SGSimone_IOC_1991.pdf.txtAMALdonadoJR_SGSimone_IOC_1991.pdf.txtExtracted texttext/plain6https://www.arca.fiocruz.br/bitstream/icict/43422/3/AMALdonadoJR_SGSimone_IOC_1991.pdf.txt6d93d3216dc4a7f5df47d4876fbec4d3MD53icict/434222020-09-18 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dc.title.pt_BR.fl_str_mv |
Identification and characterization of sex-linked proteins of Schistosoma mansoni |
title |
Identification and characterization of sex-linked proteins of Schistosoma mansoni |
spellingShingle |
Identification and characterization of sex-linked proteins of Schistosoma mansoni Maldonado Jr, Arnaldo Schistosoma mansoni Identificação Classificação Proteínas ligadas ao sexo Sex-linked proteins Schistosoma mansoni Identification Classification |
title_short |
Identification and characterization of sex-linked proteins of Schistosoma mansoni |
title_full |
Identification and characterization of sex-linked proteins of Schistosoma mansoni |
title_fullStr |
Identification and characterization of sex-linked proteins of Schistosoma mansoni |
title_full_unstemmed |
Identification and characterization of sex-linked proteins of Schistosoma mansoni |
title_sort |
Identification and characterization of sex-linked proteins of Schistosoma mansoni |
author |
Maldonado Jr, Arnaldo |
author_facet |
Maldonado Jr, Arnaldo Simone, Giovanni de |
author_role |
author |
author2 |
Simone, Giovanni de |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Maldonado Jr, Arnaldo Simone, Giovanni de |
dc.subject.other.pt_BR.fl_str_mv |
Schistosoma mansoni Identificação Classificação Proteínas ligadas ao sexo |
topic |
Schistosoma mansoni Identificação Classificação Proteínas ligadas ao sexo Sex-linked proteins Schistosoma mansoni Identification Classification |
dc.subject.en.pt_BR.fl_str_mv |
Sex-linked proteins Schistosoma mansoni Identification Classification |
description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Biologia. Rio de Janeiro, RJ, Brasil. |
publishDate |
1991 |
dc.date.issued.fl_str_mv |
1991 |
dc.date.accessioned.fl_str_mv |
2020-09-17T13:16:22Z |
dc.date.available.fl_str_mv |
2020-09-17T13:16:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MALDONADO JR., A.; SIMONE, Giovanni de. Identification and characterization of sex-linked proteins of Schistosoma mansoni. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 86, n. 1, p. 31-36, Jan./Mar. 1991. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/43422 |
dc.identifier.issn.pt_BR.fl_str_mv |
0074-0276 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S0074-02761991000100006 |
dc.identifier.eissn.none.fl_str_mv |
1678-8060 |
identifier_str_mv |
MALDONADO JR., A.; SIMONE, Giovanni de. Identification and characterization of sex-linked proteins of Schistosoma mansoni. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 86, n. 1, p. 31-36, Jan./Mar. 1991. 0074-0276 10.1590/S0074-02761991000100006 1678-8060 |
url |
https://www.arca.fiocruz.br/handle/icict/43422 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. |
publisher.none.fl_str_mv |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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Fundação Oswaldo Cruz (FIOCRUZ) |
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FIOCRUZ |
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FIOCRUZ |
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Repositório Institucional da FIOCRUZ (ARCA) |
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Repositório Institucional da FIOCRUZ (ARCA) |
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