Genetic variability of hepatitis A virus strain HAF-203 isolated in Brazil and expression of the VP1 gene in Escherichia coli

Detalhes bibliográficos
Autor(a) principal: Baptista, Marcia L.
Data de Publicação: 2006
Outros Autores: Silva, Messias, Lima, Maria Amélia de, Yoshida, Clara F. T., Gaspar, Ana Maria C., Galler, Ricardo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/38831
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.
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spelling Baptista, Marcia L.Silva, MessiasLima, Maria Amélia deYoshida, Clara F. T.Gaspar, Ana Maria C.Galler, Ricardo2019-12-26T13:25:20Z2019-12-26T13:25:20Z2006BAPTISTA, Marcia L. et al. Genetic variability of hepatitis A virus strain HAF-203 isolated in Brazil and expression of the VP1 gene in Escherichia coli. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, n. 7, p. 759-766, Nov. 2006.0074-0206https://www.arca.fiocruz.br/handle/icict/3883110.1590/s0074-027620060007000091678-8060engFundação Oswaldo Cruz. Instituto Oswaldo CruzDiagnóstico do vírus da hepatite AVírus da Hepatite AExpressão de Escherichia coliProteína recombinante do gene VP1Genoma completo (Brasil)Hepatitis A virusHAF-203 strainComplete genome (Brazil)VP1 gene recombinant proteinEscherichia coli expressionHAV diagnosisGenetic variability of hepatitis A virus strain HAF-203 isolated in Brazil and expression of the VP1 gene in Escherichia coliinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Desenvolvimento Tecnológico. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil.The hepatitis A virus (HAV) HAF-203 strain was isolated from an acute case of HAV infection. The primary isolation of HAF-203 in Brazil and its adaptation to the FRhK-4 cell lineage allowed the production of large amounts of viral particles enabling molecular characterization of the first HAV isolate in Brazil. The aim of our study was to determine the nucleotide sequence of the HAF-203 strain genome, compare it to other HAV genomes and highlight its genetic variability. The complete nucleotide sequence of the HAF-203 strain (7472 nucleotides) was compared to those obtained earlier by others for other HAV isolates. These analyses revealed 19 HAF-specific nucleotide sequence differences with 10 amino acid substitutions. Most of the non-conservative changes were located at VP1, 2C, and 3D genes, but the 3B region was the most variable. The availability of HAF-203 complementary DNA was useful for the production of the recombinant VP1 protein, which is a major determinant of viral infectivity. This recombinant protein was shown by enzyme-linked immunoassay and blotting, to be immunogenic and resemble the native protein, therefore suggesting its value as a reagent for incorporation into diagnostic tests.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/38831/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALAnaMGaspar_RicardoGaller_etal_IOC_2006.pdfAnaMGaspar_RicardoGaller_etal_IOC_2006.pdfapplication/pdf1229215https://www.arca.fiocruz.br/bitstream/icict/38831/2/AnaMGaspar_RicardoGaller_etal_IOC_2006.pdf257323348a52d4c7bc7d9cb98b9ae28fMD52TEXTAnaMGaspar_RicardoGaller_etal_IOC_2006.pdf.txtAnaMGaspar_RicardoGaller_etal_IOC_2006.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Genetic variability of hepatitis A virus strain HAF-203 isolated in Brazil and expression of the VP1 gene in Escherichia coli
title Genetic variability of hepatitis A virus strain HAF-203 isolated in Brazil and expression of the VP1 gene in Escherichia coli
spellingShingle Genetic variability of hepatitis A virus strain HAF-203 isolated in Brazil and expression of the VP1 gene in Escherichia coli
Baptista, Marcia L.
Diagnóstico do vírus da hepatite A
Vírus da Hepatite A
Expressão de Escherichia coli
Proteína recombinante do gene VP1
Genoma completo (Brasil)
Hepatitis A virus
HAF-203 strain
Complete genome (Brazil)
VP1 gene recombinant protein
Escherichia coli expression
HAV diagnosis
title_short Genetic variability of hepatitis A virus strain HAF-203 isolated in Brazil and expression of the VP1 gene in Escherichia coli
title_full Genetic variability of hepatitis A virus strain HAF-203 isolated in Brazil and expression of the VP1 gene in Escherichia coli
title_fullStr Genetic variability of hepatitis A virus strain HAF-203 isolated in Brazil and expression of the VP1 gene in Escherichia coli
title_full_unstemmed Genetic variability of hepatitis A virus strain HAF-203 isolated in Brazil and expression of the VP1 gene in Escherichia coli
title_sort Genetic variability of hepatitis A virus strain HAF-203 isolated in Brazil and expression of the VP1 gene in Escherichia coli
author Baptista, Marcia L.
author_facet Baptista, Marcia L.
Silva, Messias
Lima, Maria Amélia de
Yoshida, Clara F. T.
Gaspar, Ana Maria C.
Galler, Ricardo
author_role author
author2 Silva, Messias
Lima, Maria Amélia de
Yoshida, Clara F. T.
Gaspar, Ana Maria C.
Galler, Ricardo
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Baptista, Marcia L.
Silva, Messias
Lima, Maria Amélia de
Yoshida, Clara F. T.
Gaspar, Ana Maria C.
Galler, Ricardo
dc.subject.other.pt_BR.fl_str_mv Diagnóstico do vírus da hepatite A
Vírus da Hepatite A
Expressão de Escherichia coli
Proteína recombinante do gene VP1
Genoma completo (Brasil)
topic Diagnóstico do vírus da hepatite A
Vírus da Hepatite A
Expressão de Escherichia coli
Proteína recombinante do gene VP1
Genoma completo (Brasil)
Hepatitis A virus
HAF-203 strain
Complete genome (Brazil)
VP1 gene recombinant protein
Escherichia coli expression
HAV diagnosis
dc.subject.en.pt_BR.fl_str_mv Hepatitis A virus
HAF-203 strain
Complete genome (Brazil)
VP1 gene recombinant protein
Escherichia coli expression
HAV diagnosis
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.
publishDate 2006
dc.date.issued.fl_str_mv 2006
dc.date.accessioned.fl_str_mv 2019-12-26T13:25:20Z
dc.date.available.fl_str_mv 2019-12-26T13:25:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BAPTISTA, Marcia L. et al. Genetic variability of hepatitis A virus strain HAF-203 isolated in Brazil and expression of the VP1 gene in Escherichia coli. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, n. 7, p. 759-766, Nov. 2006.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/38831
dc.identifier.issn.pt_BR.fl_str_mv 0074-0206
dc.identifier.doi.none.fl_str_mv 10.1590/s0074-02762006000700009
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv BAPTISTA, Marcia L. et al. Genetic variability of hepatitis A virus strain HAF-203 isolated in Brazil and expression of the VP1 gene in Escherichia coli. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, n. 7, p. 759-766, Nov. 2006.
0074-0206
10.1590/s0074-02762006000700009
1678-8060
url https://www.arca.fiocruz.br/handle/icict/38831
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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