Role of western blot assay for the diagnosis of histoplasmosis in AIDS patients from a National Institute of Infectious Diseases in Rio de Janeiro, Brazil

Detalhes bibliográficos
Autor(a) principal: Almeida, Marcos Abreu
Data de Publicação: 2018
Outros Autores: Damasceno, Lisandra Serra, Pizzini, Cláudia Vera, Muniz, Mauro de Medeiros, Almeida‐Paes, Rodrigo, Zancopé‐Oliveira, Rosely Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/30788
Resumo: Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.
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spelling Almeida, Marcos AbreuDamasceno, Lisandra SerraPizzini, Cláudia VeraMuniz, Mauro de MedeirosAlmeida‐Paes, RodrigoZancopé‐Oliveira, Rosely Maria2018-12-27T12:47:29Z2018-12-27T12:47:29Z2018ALMEIDA, Marcos Abreu et al. Role of western blot assay for the diagnosis of histoplasmosis in AIDS patients from a National Institute of Infectious Diseases in Rio de Janeiro, Brazil. Mycoses, p. 1-22, Dec. 18, 2018.0933-7407https://www.arca.fiocruz.br/handle/icict/3078810.1111/myc.128771439-0507engWileyRole of western blot assay for the diagnosis of histoplasmosis in AIDS patients from a National Institute of Infectious Diseases in Rio de Janeiro, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Hospital São José de Doenças Infecciosas. Fortaleza, CE, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.BACKGROUND: Histoplasmosis is a frequent fungal infection in HIV/AIDS patients, with high morbimortality rates when diagnosis and treatment are delayed. Antibody detection, which is faster than the gold standard culture test, hasten the laboratory investigation. OBJECTIVES: To evaluate the role of WB for antibody detection in the diagnosis of histoplasmosis among HIV/AIDS patients. PATIENTS AND METHODS: Fifty patients with proven or probable histoplasmosis were included. Clinical, epidemiological and laboratory data were described in the same population after a review of their medical records. WB was performed using deglycosylated histoplasmin. RESULTS: 82% of patients were adult males and the mean age was 39.3 years. CD4+ T lymphocyte count less than 150 cells/mm3 was observed in 62% patients. Antibodies against Histoplasma capsulatum M antigen were detected in 62% of patients, and against both M and H antigens in 28% of individuals. Sera from 10% of patients were nonreactive. Histoplasmosis was the first opportunistic infection in 38% of the cases. Disseminated and pulmonary histoplasmosis occurred in 84% and 16% of patients, respectively. The overall mortality was 16%. CONCLUSION: WB could be useful for the histoplasmosis diagnosis in HIV/AIDS patients because of its easefulness and good sensitivity in a population where antibody production is hampered.HistoplasmosisAIDSAIDS patientsDiagnosisNãoNão publicadoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83104https://www.arca.fiocruz.br/bitstream/icict/30788/1/license.txt79178e5f2a0eb066867a274556814938MD51ORIGINALva_Marcos_Almeida_etal_INI_2018.pdfva_Marcos_Almeida_etal_INI_2018.pdfapplication/pdf234040https://www.arca.fiocruz.br/bitstream/icict/30788/2/va_Marcos_Almeida_etal_INI_2018.pdf6d0ea87e86dd620fd7acc1f8a25e274cMD52TEXTva_Marcos_Almeida_etal_INI_2018.pdf.txtva_Marcos_Almeida_etal_INI_2018.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Role of western blot assay for the diagnosis of histoplasmosis in AIDS patients from a National Institute of Infectious Diseases in Rio de Janeiro, Brazil
title Role of western blot assay for the diagnosis of histoplasmosis in AIDS patients from a National Institute of Infectious Diseases in Rio de Janeiro, Brazil
spellingShingle Role of western blot assay for the diagnosis of histoplasmosis in AIDS patients from a National Institute of Infectious Diseases in Rio de Janeiro, Brazil
Almeida, Marcos Abreu
Histoplasmosis
AIDS
AIDS patients
Diagnosis
title_short Role of western blot assay for the diagnosis of histoplasmosis in AIDS patients from a National Institute of Infectious Diseases in Rio de Janeiro, Brazil
title_full Role of western blot assay for the diagnosis of histoplasmosis in AIDS patients from a National Institute of Infectious Diseases in Rio de Janeiro, Brazil
title_fullStr Role of western blot assay for the diagnosis of histoplasmosis in AIDS patients from a National Institute of Infectious Diseases in Rio de Janeiro, Brazil
title_full_unstemmed Role of western blot assay for the diagnosis of histoplasmosis in AIDS patients from a National Institute of Infectious Diseases in Rio de Janeiro, Brazil
title_sort Role of western blot assay for the diagnosis of histoplasmosis in AIDS patients from a National Institute of Infectious Diseases in Rio de Janeiro, Brazil
author Almeida, Marcos Abreu
author_facet Almeida, Marcos Abreu
Damasceno, Lisandra Serra
Pizzini, Cláudia Vera
Muniz, Mauro de Medeiros
Almeida‐Paes, Rodrigo
Zancopé‐Oliveira, Rosely Maria
author_role author
author2 Damasceno, Lisandra Serra
Pizzini, Cláudia Vera
Muniz, Mauro de Medeiros
Almeida‐Paes, Rodrigo
Zancopé‐Oliveira, Rosely Maria
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Almeida, Marcos Abreu
Damasceno, Lisandra Serra
Pizzini, Cláudia Vera
Muniz, Mauro de Medeiros
Almeida‐Paes, Rodrigo
Zancopé‐Oliveira, Rosely Maria
dc.subject.en.pt_BR.fl_str_mv Histoplasmosis
AIDS
AIDS patients
Diagnosis
topic Histoplasmosis
AIDS
AIDS patients
Diagnosis
description Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-12-27T12:47:29Z
dc.date.available.fl_str_mv 2018-12-27T12:47:29Z
dc.date.issued.fl_str_mv 2018
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ALMEIDA, Marcos Abreu et al. Role of western blot assay for the diagnosis of histoplasmosis in AIDS patients from a National Institute of Infectious Diseases in Rio de Janeiro, Brazil. Mycoses, p. 1-22, Dec. 18, 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/30788
dc.identifier.issn.pt_BR.fl_str_mv 0933-7407
dc.identifier.doi.none.fl_str_mv 10.1111/myc.12877
dc.identifier.eissn.pt_BR.fl_str_mv 1439-0507
identifier_str_mv ALMEIDA, Marcos Abreu et al. Role of western blot assay for the diagnosis of histoplasmosis in AIDS patients from a National Institute of Infectious Diseases in Rio de Janeiro, Brazil. Mycoses, p. 1-22, Dec. 18, 2018.
0933-7407
10.1111/myc.12877
1439-0507
url https://www.arca.fiocruz.br/handle/icict/30788
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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