Isolation and Biochemical Characterization of a New Thrombin-Like Serine Protease from Bothrops pirajai Snake Venom
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/14078 http://dx.doi.org/10.1155/2014/595186 |
Resumo: | Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Departamento de Medicina. Universidade Federal de Rondônia. Porto Velho, RO, Brazil. |
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Zaqueo, Kayena D.Kayano, Anderson M.Simões-Silva, RodrigoMoreira-Dill, Leandro S.Fernandes, Carla F. C.Fuly, André L.Maltarollo, Vinícius G.Honório, Kathia M.Silva, Saulo L. daAcosta, GerardoCaballol, Maria Antonia O.Oliveira, Eliandre deAlbericio, FernandoCalderon, Leonardo A.Soares, Andreimar M.Stábeli, Rodrigo G.2016-05-03T13:19:15Z2016-05-03T13:19:15Z2014Stábeli, Rodrigo G., et al. Isolation and Biochemical Characterization of a New Thrombin-Like Serine Protease from Bothrops pirajai Snake Venom. BioMed Research International, v. 2014, p. 1-13, 2014.https://www.arca.fiocruz.br/handle/icict/14078http://dx.doi.org/10.1155/2014/595186engBioMed Research InternationalIsolation and Biochemical Characterization of a New Thrombin-Like Serine Protease from Bothrops pirajai Snake Venominfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Departamento de Medicina. Universidade Federal de Rondônia. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Departamento de Medicina. Universidade Federal de Rondônia. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Departamento de Medicina. Universidade Federal de Rondônia. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Departamento de Medicina. Universidade Federal de Rondônia. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Departamento de Medicina. Universidade Federal de Rondônia. Porto Velho, RO, Brazil.Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Celular e Molecular. Niteroi, RJ, Brazil.Universidade Federal do ABC. Centro de Ciências Naturais e Humanas. Santo André, SP, Brazil.Universidade Federal do ABC. Centro de Ciências Naturais e Humanas. Santo André, SP, Brazil. / Universidade de São Paulo. Escola de Artes, Ciências e Humanidades. São Paulo, SP, Brazil.Universidade Federal de São João Del Rei. Campus Alto Paraopeba. Ouro Branco, MG, Brazil.Institute for Research in Biomedicine. Barcelona, Spain. / Barcelona Science Park. Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina. Barcelona, Spain.Barcelona Science Park. Proteomic Platform. Barcelona, Spain.Barcelona Science Park. Proteomic Platform. Barcelona, Spain.Institute for Research in Biomedicine. Barcelona, Spain. / Barcelona Science Park. Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina. Barcelona, Spain. / University of Barcelona. Department of Organic Chemistry. Barcelona, Spain. / University of KwaZulu Natal. School of Chemistry. Durban, South Africa.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Departamento de Medicina. Universidade Federal de Rondônia. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Departamento de Medicina. Universidade Federal de Rondônia. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Departamento de Medicina. Universidade Federal de Rondônia. Porto Velho, RO, Brazil.This paper presents a novel serine protease (SP) isolated from Bothrops pirajai, a venomous snake found solely in Brazil that belongs to the Viperidae family. The identified SP, named BpirSP-39, was isolated by three chromatographic steps (size exclusion, bioaffinity, and reverse phase chromatographies). The molecular mass of BpirSP-39 was estimated by SDS-PAGE and confirmed by mass spectrometry (39,408.32Da). The protein was able to form fibrin networks, which was not observed in the presence of serine protease inhibitors, such as phenylmethylsulfonyl fluoride (PMSF). Furthermore, BpirSP-39 presented considerable thermal stability and was apparently able to activate factor XIII of the blood coagulation cascade, un like most serine proteases. BpirSP-39 was capable of hydrolyzing different chromogenic substrates tested (S-2222, S-2302, and S-2238) while Cu2+ significantly diminished BspirSP-39 activity on the three tested substrates. The enzyme promoted platelet aggregation and also exhibited fibrinogenolytic, fibrinolytic, gelatinolytic, and amidolytic activities. The multiple alignment showed high sequence similarity to other thrombin-like enzymes from snake venoms. These results allow us to conclude that a new SP was isolated from Bothrops pirajai snake venom.Thrombin-LikeBothrops pirajaiSnake Venominfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALIsolation and Biochemical Characterization of a New Thrombin-Like Serine Protease from Bothrops pirajai Snake Venom.pdfIsolation and Biochemical Characterization of a New Thrombin-Like Serine Protease from Bothrops pirajai Snake Venom.pdfapplication/pdf4262246https://www.arca.fiocruz.br/bitstream/icict/14078/2/Isolation%20and%20Biochemical%20Characterization%20of%20a%20New%20Thrombin-Like%20Serine%20Protease%20from%20Bothrops%20pirajai%20Snake%20Venom.pdfd6e3f5bb80b013a337bed40150e217ddMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/14078/3/license.txt5a560609d32a3863062d77ff32785d58MD53TEXTIsolation and Biochemical Characterization of a New Thrombin-Like Serine Protease from Bothrops pirajai Snake Venom.pdf.txtIsolation and Biochemical Characterization of a New Thrombin-Like Serine Protease from Bothrops pirajai Snake Venom.pdf.txtExtracted texttext/plain60759https://www.arca.fiocruz.br/bitstream/icict/14078/4/Isolation%20and%20Biochemical%20Characterization%20of%20a%20New%20Thrombin-Like%20Serine%20Protease%20from%20Bothrops%20pirajai%20Snake%20Venom.pdf.txt6615ad6aa5511c0b211d46f44a0a2353MD54icict/140782018-04-02 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| dc.title.pt_BR.fl_str_mv |
Isolation and Biochemical Characterization of a New Thrombin-Like Serine Protease from Bothrops pirajai Snake Venom |
| title |
Isolation and Biochemical Characterization of a New Thrombin-Like Serine Protease from Bothrops pirajai Snake Venom |
| spellingShingle |
Isolation and Biochemical Characterization of a New Thrombin-Like Serine Protease from Bothrops pirajai Snake Venom Zaqueo, Kayena D. Thrombin-Like Bothrops pirajai Snake Venom |
| title_short |
Isolation and Biochemical Characterization of a New Thrombin-Like Serine Protease from Bothrops pirajai Snake Venom |
| title_full |
Isolation and Biochemical Characterization of a New Thrombin-Like Serine Protease from Bothrops pirajai Snake Venom |
| title_fullStr |
Isolation and Biochemical Characterization of a New Thrombin-Like Serine Protease from Bothrops pirajai Snake Venom |
| title_full_unstemmed |
Isolation and Biochemical Characterization of a New Thrombin-Like Serine Protease from Bothrops pirajai Snake Venom |
| title_sort |
Isolation and Biochemical Characterization of a New Thrombin-Like Serine Protease from Bothrops pirajai Snake Venom |
| author |
Zaqueo, Kayena D. |
| author_facet |
Zaqueo, Kayena D. Kayano, Anderson M. Simões-Silva, Rodrigo Moreira-Dill, Leandro S. Fernandes, Carla F. C. Fuly, André L. Maltarollo, Vinícius G. Honório, Kathia M. Silva, Saulo L. da Acosta, Gerardo Caballol, Maria Antonia O. Oliveira, Eliandre de Albericio, Fernando Calderon, Leonardo A. Soares, Andreimar M. Stábeli, Rodrigo G. |
| author_role |
author |
| author2 |
Kayano, Anderson M. Simões-Silva, Rodrigo Moreira-Dill, Leandro S. Fernandes, Carla F. C. Fuly, André L. Maltarollo, Vinícius G. Honório, Kathia M. Silva, Saulo L. da Acosta, Gerardo Caballol, Maria Antonia O. Oliveira, Eliandre de Albericio, Fernando Calderon, Leonardo A. Soares, Andreimar M. Stábeli, Rodrigo G. |
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author author author author author author author author author author author author author author author |
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Zaqueo, Kayena D. Kayano, Anderson M. Simões-Silva, Rodrigo Moreira-Dill, Leandro S. Fernandes, Carla F. C. Fuly, André L. Maltarollo, Vinícius G. Honório, Kathia M. Silva, Saulo L. da Acosta, Gerardo Caballol, Maria Antonia O. Oliveira, Eliandre de Albericio, Fernando Calderon, Leonardo A. Soares, Andreimar M. Stábeli, Rodrigo G. |
| dc.subject.en.pt_BR.fl_str_mv |
Thrombin-Like Bothrops pirajai Snake Venom |
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Thrombin-Like Bothrops pirajai Snake Venom |
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Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Departamento de Medicina. Universidade Federal de Rondônia. Porto Velho, RO, Brazil. |
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2014 |
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2014 |
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2016-05-03T13:19:15Z |
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2016-05-03T13:19:15Z |
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Stábeli, Rodrigo G., et al. Isolation and Biochemical Characterization of a New Thrombin-Like Serine Protease from Bothrops pirajai Snake Venom. BioMed Research International, v. 2014, p. 1-13, 2014. |
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https://www.arca.fiocruz.br/handle/icict/14078 |
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http://dx.doi.org/10.1155/2014/595186 |
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Stábeli, Rodrigo G., et al. Isolation and Biochemical Characterization of a New Thrombin-Like Serine Protease from Bothrops pirajai Snake Venom. BioMed Research International, v. 2014, p. 1-13, 2014. |
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