Dual RNA-Seq of Human Leprosy Lesions Identifies Bacterial Determinants Linked to Host Immune Response

Detalhes bibliográficos
Autor(a) principal: Montoya, Dennis J.
Data de Publicação: 2019
Outros Autores: Andrade, Priscila, Silva, Bruno J. A., Teles, Rosane M. B., Ma, Feiyang, Bryson, Bryan, Sadanand, Saheli, Noel, Teia, Lu, Jing, Sarno, Euzenir N., Arnvig, Kristine B., Young, Douglas, Lahiri, Ramanuj, Williams, Diana L., Fortune, Sarah, Bloom, Barry R., Pellegrini, Matteo, Modlin, Robert L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/35255
Resumo: University of California Los Angeles. Department of Molecular, Cell, and Developmental Biology. Los Angeles, CA, USA.
id CRUZ_7946e3402a0da5c910985d6441b964b7
oai_identifier_str oai:www.arca.fiocruz.br:icict/35255
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Montoya, Dennis J.Andrade, PriscilaSilva, Bruno J. A.Teles, Rosane M. B.Ma, FeiyangBryson, BryanSadanand, SaheliNoel, TeiaLu, JingSarno, Euzenir N.Arnvig, Kristine B.Young, DouglasLahiri, RamanujWilliams, Diana L.Fortune, SarahBloom, Barry R.Pellegrini, MatteoModlin, Robert L.2019-09-02T16:17:33Z2019-09-02T16:17:33Z2019MONTOYA, Dennis J. et al. Dual RNA-Seq of Human Leprosy Lesions Identifies Bacterial Determinants Linked to Host Immune Response. Cell Reports, v. 26, n. 13, p. 3574-3585, Mar. 2019.2211-1247https://www.arca.fiocruz.br/handle/icict/3525510.1016/j.celrep.2019.02.1092211-1247engElsevierHanseníase humanaRNA-Seq duploDeterminantes bacterianosResposta imune do hospedeiroMycobacterium lepraeHuman LeprosyBacterial DeterminantsHost Immune ResponseDual RNA-SeqMycobacterium lepraeDual RNA-Seq of Human Leprosy Lesions Identifies Bacterial Determinants Linked to Host Immune Responseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversity of California Los Angeles. Department of Molecular, Cell, and Developmental Biology. Los Angeles, CA, USA.David Geffen School of Medicine. Division of Dermatology. Los Angeles, CA, USA.David Geffen School of Medicine. Division of Dermatology. Los Angeles, CA, USA.David Geffen School of Medicine. Division of Dermatology. Los Angeles, CA, USA.University of California Los Angeles. Department of Molecular, Cell, and Developmental Biology. Los Angeles, CA, USA.Harvard T.H. Chan School of Public Health. Department of Immunology and Infectious Diseases. Boston, MA, USA.Ragon Institute of MGH, MIT and Harvard. Cambridge, MA, USA.University of California Los Angeles. Department of Molecular, Cell, and Developmental Biology. Los Angeles, CA, USA.University of California Los Angeles. Department of Molecular, Cell, and Developmental Biology. Los Angeles, CA, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Micobacterioses. Rio de Janeiro, RJ, Brasil.University College London. Institute for Structural and Molecular Biology. London, UK.National Institute for Medical Research. Mycobacterial Research Division. London, UK / The Francis Crick Institute. Mill Hill Laboratory. The Ridgeway. London, USA.Health Resources and Services Administration. National Hansen’s Disease Program. Baton Rouge, LA, USA.Health Resources and Services Administration. National Hansen’s Disease Program. Baton Rouge, LA, USA / Louisiana State University. Department of Pathobiological Sciences. Baton Rouge, LA, USA.Harvard T.H. Chan School of Public Health. Department of Immunology and Infectious Diseases. Boston, MA, USA.Harvard T.H. Chan School of Public Health. Department of Immunology and Infectious Diseases. Boston, MA, USA.University of California Los Angeles. Department of Molecular, Cell, and Developmental Biology. Los Angeles, CA, USA.David Geffen School of Medicine. Division of Dermatology. Los Angeles, CA, USA.To understand how the interaction between an intracellular bacterium and the host immune system contributes to outcome at the site of infection, we studied leprosy, a disease that forms a clinical spectrum, in which progressive infection by the intracellular bacterium Mycobacterium leprae is characterized by the production of type I IFNs and antibody production. Dual RNA-seq on patient lesions identifies two independent molecular measures of M. leprae, each of which correlates with distinct aspects of the host immune response. The fraction of bacterial transcripts, reflecting bacterial burden, correlates with a host type I IFN gene signature, known to inhibit antimicrobial responses. Second, the bacterial mRNA:rRNA ratio, reflecting bacterial viability, links bacterial heat shock proteins with the BAFF-BCMA host antibody response pathway. Our findings provide a platform for the interrogation of host and pathogen transcriptomes at the site of infection, allowing insight into mechanisms of inflammation in human disease.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/35255/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALEuzenirSarno_BrunoSilva_etal_IOC_2019.pdfEuzenirSarno_BrunoSilva_etal_IOC_2019.pdfapplication/pdf1955779https://www.arca.fiocruz.br/bitstream/icict/35255/2/EuzenirSarno_BrunoSilva_etal_IOC_2019.pdff213bc374d5b73657c80706a08fc8a7dMD52TEXTEuzenirSarno_BrunoSilva_etal_IOC_2019.pdf.txtEuzenirSarno_BrunoSilva_etal_IOC_2019.pdf.txtExtracted texttext/plain75215https://www.arca.fiocruz.br/bitstream/icict/35255/3/EuzenirSarno_BrunoSilva_etal_IOC_2019.pdf.txt6577fd4905d71891b60c56aab36d0a1bMD53icict/352552019-09-06 20:15:11.282oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-09-06T23:15:11Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Dual RNA-Seq of Human Leprosy Lesions Identifies Bacterial Determinants Linked to Host Immune Response
title Dual RNA-Seq of Human Leprosy Lesions Identifies Bacterial Determinants Linked to Host Immune Response
spellingShingle Dual RNA-Seq of Human Leprosy Lesions Identifies Bacterial Determinants Linked to Host Immune Response
Montoya, Dennis J.
Hanseníase humana
RNA-Seq duplo
Determinantes bacterianos
Resposta imune do hospedeiro
Mycobacterium leprae
Human Leprosy
Bacterial Determinants
Host Immune Response
Dual RNA-Seq
Mycobacterium leprae
title_short Dual RNA-Seq of Human Leprosy Lesions Identifies Bacterial Determinants Linked to Host Immune Response
title_full Dual RNA-Seq of Human Leprosy Lesions Identifies Bacterial Determinants Linked to Host Immune Response
title_fullStr Dual RNA-Seq of Human Leprosy Lesions Identifies Bacterial Determinants Linked to Host Immune Response
title_full_unstemmed Dual RNA-Seq of Human Leprosy Lesions Identifies Bacterial Determinants Linked to Host Immune Response
title_sort Dual RNA-Seq of Human Leprosy Lesions Identifies Bacterial Determinants Linked to Host Immune Response
author Montoya, Dennis J.
author_facet Montoya, Dennis J.
Andrade, Priscila
Silva, Bruno J. A.
Teles, Rosane M. B.
Ma, Feiyang
Bryson, Bryan
Sadanand, Saheli
Noel, Teia
Lu, Jing
Sarno, Euzenir N.
Arnvig, Kristine B.
Young, Douglas
Lahiri, Ramanuj
Williams, Diana L.
Fortune, Sarah
Bloom, Barry R.
Pellegrini, Matteo
Modlin, Robert L.
author_role author
author2 Andrade, Priscila
Silva, Bruno J. A.
Teles, Rosane M. B.
Ma, Feiyang
Bryson, Bryan
Sadanand, Saheli
Noel, Teia
Lu, Jing
Sarno, Euzenir N.
Arnvig, Kristine B.
Young, Douglas
Lahiri, Ramanuj
Williams, Diana L.
Fortune, Sarah
Bloom, Barry R.
Pellegrini, Matteo
Modlin, Robert L.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Montoya, Dennis J.
Andrade, Priscila
Silva, Bruno J. A.
Teles, Rosane M. B.
Ma, Feiyang
Bryson, Bryan
Sadanand, Saheli
Noel, Teia
Lu, Jing
Sarno, Euzenir N.
Arnvig, Kristine B.
Young, Douglas
Lahiri, Ramanuj
Williams, Diana L.
Fortune, Sarah
Bloom, Barry R.
Pellegrini, Matteo
Modlin, Robert L.
dc.subject.other.pt_BR.fl_str_mv Hanseníase humana
RNA-Seq duplo
Determinantes bacterianos
Resposta imune do hospedeiro
Mycobacterium leprae
topic Hanseníase humana
RNA-Seq duplo
Determinantes bacterianos
Resposta imune do hospedeiro
Mycobacterium leprae
Human Leprosy
Bacterial Determinants
Host Immune Response
Dual RNA-Seq
Mycobacterium leprae
dc.subject.en.pt_BR.fl_str_mv Human Leprosy
Bacterial Determinants
Host Immune Response
Dual RNA-Seq
Mycobacterium leprae
description University of California Los Angeles. Department of Molecular, Cell, and Developmental Biology. Los Angeles, CA, USA.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-09-02T16:17:33Z
dc.date.available.fl_str_mv 2019-09-02T16:17:33Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MONTOYA, Dennis J. et al. Dual RNA-Seq of Human Leprosy Lesions Identifies Bacterial Determinants Linked to Host Immune Response. Cell Reports, v. 26, n. 13, p. 3574-3585, Mar. 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/35255
dc.identifier.issn.pt_BR.fl_str_mv 2211-1247
dc.identifier.doi.none.fl_str_mv 10.1016/j.celrep.2019.02.109
dc.identifier.eissn.none.fl_str_mv 2211-1247
identifier_str_mv MONTOYA, Dennis J. et al. Dual RNA-Seq of Human Leprosy Lesions Identifies Bacterial Determinants Linked to Host Immune Response. Cell Reports, v. 26, n. 13, p. 3574-3585, Mar. 2019.
2211-1247
10.1016/j.celrep.2019.02.109
url https://www.arca.fiocruz.br/handle/icict/35255
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/35255/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/35255/2/EuzenirSarno_BrunoSilva_etal_IOC_2019.pdf
https://www.arca.fiocruz.br/bitstream/icict/35255/3/EuzenirSarno_BrunoSilva_etal_IOC_2019.pdf.txt
bitstream.checksum.fl_str_mv 5a560609d32a3863062d77ff32785d58
f213bc374d5b73657c80706a08fc8a7d
6577fd4905d71891b60c56aab36d0a1b
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798324612333830144

Registros relacionados