Understanding the mechanisms controlling Leishmania amazonensis infection In vitro: the role of LTB4 derived from human neutrophils.

Detalhes bibliográficos
Autor(a) principal: Tavares, Natalia Machado
Data de Publicação: 2014
Outros Autores: Santos, Théo de Araújo, Afonso, Lilian Maria, Nogueira, Paula Monalisa, Lopes, Ulisses Gazos, Soares, Rodrigo Pedro, Bozza, Patrícia Torres, Bandeira-Melo, Christianne, Borges, Valeria de Matos, Brodskyn, Claudia Ida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8381
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil
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spelling Tavares, Natalia MachadoSantos, Théo de AraújoAfonso, Lilian MariaNogueira, Paula MonalisaLopes, Ulisses GazosSoares, Rodrigo PedroBozza, Patrícia TorresBandeira-Melo, ChristianneBorges, Valeria de MatosBrodskyn, Claudia Ida2014-09-15T18:10:54Z2014-09-15T18:10:54Z2014TAVARES, N. M. et al. Understanding the mechanisms controlling Leishmania amazonensis infection In vitro: the role of LTB4 derived from human neutrophils. Journal of Infectious Disease, v. 210, n. 4, p. 656-666, 2014.1537-6613https://www.arca.fiocruz.br/handle/icict/838110.1093/infdis/jiu158engOxford University PressUnderstanding the mechanisms controlling Leishmania amazonensis infection In vitro: the role of LTB4 derived from human neutrophils.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Belo Horizonte, MG, BrasilFederal University of Rio de Janeiro. UFRJ.Carlos Chagas Filho Institute. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, BrasilFederal University of Rio de Janeiro. UFRJ.Carlos Chagas Filho Institute. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / National Institute of Science and Technology for Immunology Investigation (iii–INCT). Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / National Institute of Science and Technology for Immunology Investigation (iii–INCT). Salvador, BA, BrasilNeutrophils are rapidly recruited to the site of Leishmania infection and play an active role in capturing and killing parasites. They are the main source of leukotriene B4 (LTB4), a potent proinflammatory lipid mediator. However, the role of LTB4 in neutrophil infection by Leishmania amazonensis is not clear. In this study, we show that L. amazonensis or its lipophosphoglycan can induce neutrophil activation, degranulation, and LTB4 production. Using pharmacological inhibitors of leukotriene synthesis, our findings reveal an LTB4-driven autocrine/ paracrine regulatory effect. In particular, neutrophil-derived LTB4 controls L. amazonensis killing, degranulation, and reactive oxygen species production. In addition, L. amazonensis infection induces an early increase in Toll-like receptor 2 expression, which facilitates parasite internalization. Nuclear factor kappa B (NFkB) pathway activation represents a required upstream event for L. amazonensis–induced LTB4 synthesis. These leishmanicidal mechanisms mediated by neutrophil-derived LTB4 act through activation of its receptor, B leukotriene receptor 1 (BLT1)Leishmania amazonensisLPGHuman neutrophilsLeukotriene B4TLR2NFkBinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8381/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALTavares NM Understanding the mechanisms.....pdfTavares NM Understanding the mechanisms.....pdfapplication/pdf856640https://www.arca.fiocruz.br/bitstream/icict/8381/2/Tavares%20NM%20Understanding%20the%20mechanisms.....pdf51c5935f5e761744af4ab01f86804777MD52TEXTTavares NM Understanding the mechanisms.....pdf.txtTavares NM Understanding the mechanisms.....pdf.txtExtracted texttext/plain46175https://www.arca.fiocruz.br/bitstream/icict/8381/3/Tavares%20NM%20Understanding%20the%20mechanisms.....pdf.txt59db7bbfe503bbf52de69d521d36f4a5MD53icict/83812023-09-14 15:53:39.682oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-14T18:53:39Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Understanding the mechanisms controlling Leishmania amazonensis infection In vitro: the role of LTB4 derived from human neutrophils.
title Understanding the mechanisms controlling Leishmania amazonensis infection In vitro: the role of LTB4 derived from human neutrophils.
spellingShingle Understanding the mechanisms controlling Leishmania amazonensis infection In vitro: the role of LTB4 derived from human neutrophils.
Tavares, Natalia Machado
Leishmania amazonensis
LPG
Human neutrophils
Leukotriene B4
TLR2
NFkB
title_short Understanding the mechanisms controlling Leishmania amazonensis infection In vitro: the role of LTB4 derived from human neutrophils.
title_full Understanding the mechanisms controlling Leishmania amazonensis infection In vitro: the role of LTB4 derived from human neutrophils.
title_fullStr Understanding the mechanisms controlling Leishmania amazonensis infection In vitro: the role of LTB4 derived from human neutrophils.
title_full_unstemmed Understanding the mechanisms controlling Leishmania amazonensis infection In vitro: the role of LTB4 derived from human neutrophils.
title_sort Understanding the mechanisms controlling Leishmania amazonensis infection In vitro: the role of LTB4 derived from human neutrophils.
author Tavares, Natalia Machado
author_facet Tavares, Natalia Machado
Santos, Théo de Araújo
Afonso, Lilian Maria
Nogueira, Paula Monalisa
Lopes, Ulisses Gazos
Soares, Rodrigo Pedro
Bozza, Patrícia Torres
Bandeira-Melo, Christianne
Borges, Valeria de Matos
Brodskyn, Claudia Ida
author_role author
author2 Santos, Théo de Araújo
Afonso, Lilian Maria
Nogueira, Paula Monalisa
Lopes, Ulisses Gazos
Soares, Rodrigo Pedro
Bozza, Patrícia Torres
Bandeira-Melo, Christianne
Borges, Valeria de Matos
Brodskyn, Claudia Ida
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Tavares, Natalia Machado
Santos, Théo de Araújo
Afonso, Lilian Maria
Nogueira, Paula Monalisa
Lopes, Ulisses Gazos
Soares, Rodrigo Pedro
Bozza, Patrícia Torres
Bandeira-Melo, Christianne
Borges, Valeria de Matos
Brodskyn, Claudia Ida
dc.subject.en.pt_BR.fl_str_mv Leishmania amazonensis
LPG
Human neutrophils
Leukotriene B4
TLR2
NFkB
topic Leishmania amazonensis
LPG
Human neutrophils
Leukotriene B4
TLR2
NFkB
description Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil
publishDate 2014
dc.date.accessioned.fl_str_mv 2014-09-15T18:10:54Z
dc.date.available.fl_str_mv 2014-09-15T18:10:54Z
dc.date.issued.fl_str_mv 2014
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv TAVARES, N. M. et al. Understanding the mechanisms controlling Leishmania amazonensis infection In vitro: the role of LTB4 derived from human neutrophils. Journal of Infectious Disease, v. 210, n. 4, p. 656-666, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8381
dc.identifier.issn.none.fl_str_mv 1537-6613
dc.identifier.doi.none.fl_str_mv 10.1093/infdis/jiu158
identifier_str_mv TAVARES, N. M. et al. Understanding the mechanisms controlling Leishmania amazonensis infection In vitro: the role of LTB4 derived from human neutrophils. Journal of Infectious Disease, v. 210, n. 4, p. 656-666, 2014.
1537-6613
10.1093/infdis/jiu158
url https://www.arca.fiocruz.br/handle/icict/8381
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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