Prevalence of baseline NS3 resistance-associated substitutions (RASs)on treatment with protease inhibitors inpatients infected with HCV genotype 1

Detalhes bibliográficos
Autor(a) principal: Costa, Vanessa Duarte
Data de Publicação: 2019
Outros Autores: Delvaux, Nathália, Brandão-Mello, Carlos Eduardo, Nunes, Estevão Portela, Sousa, Paulo Sérgio Fonseca de, Rodrigues, Lia Laura Lewis Ximenez de Souza, Lampe, Elisabeth, Mello, Francisco Campello do Amaral
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/32244
Resumo: 2020-03-15
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spelling Costa, Vanessa DuarteDelvaux, NatháliaBrandão-Mello, Carlos EduardoNunes, Estevão PortelaSousa, Paulo Sérgio Fonseca deRodrigues, Lia Laura Lewis Ximenez de SouzaLampe, ElisabethMello, Francisco Campello do Amaral2019-03-27T13:26:08Z2019-03-27T13:26:08Z2019COSTA, Vanessa Duarte et al. Prevalence of baseline NS3 resistance-associated substitutions (RASs) on treatment with protease inhibitors in patients infected with HCV genotype 1. Clinics and Research in Hepatology and Gastroenterology, p. 1-7, 2019.2210-7401https://www.arca.fiocruz.br/handle/icict/3224410.1016/j.clinre.2019.02.0092210-741XengElsevierPrevalence of baseline NS3 resistance-associated substitutions (RASs)on treatment with protease inhibitors inpatients infected with HCV genotype 1info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-03-15Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.Hospital Universitário Gaffrée e Guinle. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.Background and aims: Treatment for hepatitis C has evolved significantly with the licensing of direct-acting antiviral drugs (DAAs). However, one of the limiting factors of the effectiveness of antiviral therapy with protease inhibitors (PIs) is the emergence of resistance caused by point mutations. The aim of this study was to determine the prevalence of resistance-associated substitutions (RASs) in HCV NS3 gene in patients infected with genotype 1 before therapy with simeprevir. Methods: A total of 73 serum samples from 15 treatment-experienced patients with boceprevir/telaprevir and 58 DAA-naïve patients were collected before therapy with DAAs simeprevir, daclatasvir and/or sofosbuvir. Presence of baseline resistance-associated substitutions (RAS) in the serine protease domain of HCV NS3 was analyzed by nucleotide sequencing followed by amino acid deduction. Results: Overall RAS prevalence in this study was 13.7% (10/73). RAS prevalence for HCV subtype 1b was 17.4% (4/23) while for HCV subtype 1a was 12% (6/50). Primary mutations V36M/L and R155K were observed only in HCV subtype 1a, whereas T54S and Q80K were identified only in HCV subtype 1b. RAS V36M, which is related to reduction of susceptibility to second-generation PIs, was the most frequent in the study (6.9%; 5/73). Conclusions: Our results indicated that Brazilian isolates of HCV present a distinct pattern of RAS depending on the infecting viral subtype. In contrast to data from other countries, RAS Q80K prevalence in Brazil is low in HCV subtype 1a. This study improves the knowledge of genetic barrier for resistance to PIs involving RASs in chronically infected patients and its possible impact on an unsuccessful treatment outcome, information that might be crucial to upcoming decisions of incorporation of new DAAs in Brazilian guidelines of antiviral therapy against HCV infection.HCVRASProtease inhibitorsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83104https://www.arca.fiocruz.br/bitstream/icict/32244/1/license.txt79178e5f2a0eb066867a274556814938MD51ORIGINALve_Costa_Vanessa_etal_INI_2019.pdfve_Costa_Vanessa_etal_INI_2019.pdfapplication/pdf669414https://www.arca.fiocruz.br/bitstream/icict/32244/2/ve_Costa_Vanessa_etal_INI_2019.pdf337a705652a249412706540e45e5b055MD52TEXTve_Costa_Vanessa_etal_INI_2019.pdf.txtve_Costa_Vanessa_etal_INI_2019.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Prevalence of baseline NS3 resistance-associated substitutions (RASs)on treatment with protease inhibitors inpatients infected with HCV genotype 1
title Prevalence of baseline NS3 resistance-associated substitutions (RASs)on treatment with protease inhibitors inpatients infected with HCV genotype 1
spellingShingle Prevalence of baseline NS3 resistance-associated substitutions (RASs)on treatment with protease inhibitors inpatients infected with HCV genotype 1
Costa, Vanessa Duarte
HCV
RAS
Protease inhibitors
title_short Prevalence of baseline NS3 resistance-associated substitutions (RASs)on treatment with protease inhibitors inpatients infected with HCV genotype 1
title_full Prevalence of baseline NS3 resistance-associated substitutions (RASs)on treatment with protease inhibitors inpatients infected with HCV genotype 1
title_fullStr Prevalence of baseline NS3 resistance-associated substitutions (RASs)on treatment with protease inhibitors inpatients infected with HCV genotype 1
title_full_unstemmed Prevalence of baseline NS3 resistance-associated substitutions (RASs)on treatment with protease inhibitors inpatients infected with HCV genotype 1
title_sort Prevalence of baseline NS3 resistance-associated substitutions (RASs)on treatment with protease inhibitors inpatients infected with HCV genotype 1
author Costa, Vanessa Duarte
author_facet Costa, Vanessa Duarte
Delvaux, Nathália
Brandão-Mello, Carlos Eduardo
Nunes, Estevão Portela
Sousa, Paulo Sérgio Fonseca de
Rodrigues, Lia Laura Lewis Ximenez de Souza
Lampe, Elisabeth
Mello, Francisco Campello do Amaral
author_role author
author2 Delvaux, Nathália
Brandão-Mello, Carlos Eduardo
Nunes, Estevão Portela
Sousa, Paulo Sérgio Fonseca de
Rodrigues, Lia Laura Lewis Ximenez de Souza
Lampe, Elisabeth
Mello, Francisco Campello do Amaral
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Costa, Vanessa Duarte
Delvaux, Nathália
Brandão-Mello, Carlos Eduardo
Nunes, Estevão Portela
Sousa, Paulo Sérgio Fonseca de
Rodrigues, Lia Laura Lewis Ximenez de Souza
Lampe, Elisabeth
Mello, Francisco Campello do Amaral
dc.subject.en.pt_BR.fl_str_mv HCV
RAS
Protease inhibitors
topic HCV
RAS
Protease inhibitors
description 2020-03-15
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-03-27T13:26:08Z
dc.date.available.fl_str_mv 2019-03-27T13:26:08Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv COSTA, Vanessa Duarte et al. Prevalence of baseline NS3 resistance-associated substitutions (RASs) on treatment with protease inhibitors in patients infected with HCV genotype 1. Clinics and Research in Hepatology and Gastroenterology, p. 1-7, 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/32244
dc.identifier.issn.pt_BR.fl_str_mv 2210-7401
dc.identifier.doi.none.fl_str_mv 10.1016/j.clinre.2019.02.009
dc.identifier.eissn.none.fl_str_mv 2210-741X
identifier_str_mv COSTA, Vanessa Duarte et al. Prevalence of baseline NS3 resistance-associated substitutions (RASs) on treatment with protease inhibitors in patients infected with HCV genotype 1. Clinics and Research in Hepatology and Gastroenterology, p. 1-7, 2019.
2210-7401
10.1016/j.clinre.2019.02.009
2210-741X
url https://www.arca.fiocruz.br/handle/icict/32244
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/32244/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/32244/2/ve_Costa_Vanessa_etal_INI_2019.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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