Immunohistochemical detection of Ki-67 is not associated with tumor-infiltrating macrophages and cyclooxygenase-2 in oral squamous cell carcinoma.

Detalhes bibliográficos
Autor(a) principal: Bôas, Deise Souza Vilas
Data de Publicação: 2010
Outros Autores: Takiya, Christina Maeda, Sampaio, Tatiana Lobo Coelho, Ribeiro, Leonardo Campos Monção, Ramos, Eduardo Antônio Gonçalves, Cabral, Márcia Grillo, Santos, Jean Nunes dos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8926
Resumo: Federal University of Bahia. Institute of Health Sciences. Department of Bio-morphology. Salvador, BA, Brasil / Federal University of Rio de Janeiro. Institute of Biomedical Sciences. Department of Histology and Embryology. Rio de Janeiro, RJ, Brasil
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spelling Bôas, Deise Souza VilasTakiya, Christina MaedaSampaio, Tatiana Lobo CoelhoRibeiro, Leonardo Campos MonçãoRamos, Eduardo Antônio GonçalvesCabral, Márcia GrilloSantos, Jean Nunes dos2014-11-20T18:30:16Z2014-11-20T18:30:16Z2010BOAS, D. S. V. et al. Immunohistochemical detection of Ki-67 is not associated with tumor-infiltrating macrophages and cyclooxygenase-2 in oral squamous cell carcinoma. Journal of Oral Pathology & Medicine, v. 39, 7, p. 565-570, 2010.1600-0714https://www.arca.fiocruz.br/handle/icict/892610.1111/j.1600-0714.2010.00883.xengJohn Wiley & Sons LtdImmunohistochemical detection of Ki-67 is not associated with tumor-infiltrating macrophages and cyclooxygenase-2 in oral squamous cell carcinoma.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFederal University of Bahia. Institute of Health Sciences. Department of Bio-morphology. Salvador, BA, Brasil / Federal University of Rio de Janeiro. Institute of Biomedical Sciences. Department of Histology and Embryology. Rio de Janeiro, RJ, BrasilFederal University of Rio de Janeiro. Institute of Biomedical Sciences. Department of Histology and Embryology. Rio de Janeiro, RJ, BrasilFederal University of Rio de Janeiro. Institute of Biomedical Sciences. Department of Histology and Embryology. Rio de Janeiro, RJ, BrasilFederal University of Rio de Janeiro. Institute of Biomedical Sciences. Department of Histology and Embryology. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFederal University of Rio de Janeiro. School of Dentistry. Department of Pathology and Oral Diagnosis. Rio de Janeiro, RJ, BrasilFederal University of Bahia. School of Dentistry. Laboratory of Oral Surgical Pathology. Salvador, BA, BrasilBACKGROUND: An inflammatory component consisting of cells and chemical mediators may influence the proliferation and dissemination of the oral squamous cell carcinoma (OSCC). In the present study, we evaluated the possible relationship between Ki-67, tumor-associated macrophages (TAMs), and COX-2 in OSCCs. In addition, the immunodetection of these proteins was associated with different histological grades of malignancy, including invasive and in situ tumors. METHODS: Twenty-seven OSCC cases were examined by light microscopy using criteria adopted WHO, and immunohistochemistry for Ki-67, CD68, and COX-2 using EnVision System in invasive and in situ lesions. Immunohistochemical detection of these proteins was assessed and scored for COX-2, and results were compared with their histological grades of malignancy. RESULTS: A correlation between Ki-67, COX-2, and CD68 was not found. Histological grade of malignancy (HDM) was associated with the Ki-67 immunostaining (P = 0.00), but this was not observed regarding both CD68 (P = 0.51) and COX-2 (P = 0.89). Furthermore, there was a COX-2 overexpression in 62.96% of the sample, and a high density of TAMs in both OSCCs and in situ carcinomas. CONCLUSIONS: Imunolabeling for Ki-67 was directly correlated with less-differentiated tumors, suggesting that this marker may contribute to understand the biological behavior of OSCC, and help to distinguish risk groups of OSCC. Furthermore, the lack of correlation between Ki-67, COX-2, and CD68 indicates that the latter two markers may play a pivotal role in oral carcinogenesis. However, further studies are needed to clarify their contribution for cell proliferation and tumor differentiation.Cyclooxygenase-2ImunohistochemistryKi-67MacrophagesSquamous cell carcinomaCarcinoma de Células Escamosas/patologiaCiclo-Oxigenase 2/análiseAntígeno Ki-67/análiseMacrófagos/patologiaNeoplasias Bucais/patologiaAntígenos CD/análiseAntígenos de Diferenciação Mielomonocítica/análiseCarcinoma in Situ/enzimologiaCarcinoma de Células Escamosas/enzimologiaNúcleo Celular/ultraestruturaCorantes/uso diagnósticoCitoplasma/ultraestruturaCélulas Epiteliais/enzimologiaHumanosImuno-HistoquímicaNeoplasias Bucais/enzimologiaInvasividade NeoplásicaMarcadores Biológicos de Tumor/análiseinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8926/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALBoas DSV Immunohistochemical....pdfBoas DSV Immunohistochemical....pdfapplication/pdf439218https://www.arca.fiocruz.br/bitstream/icict/8926/2/Boas%20DSV%20Immunohistochemical....pdff569901cc76550ae95534d03260b9b8eMD52TEXTBoas DSV Immunohistochemical....pdf.txtBoas DSV Immunohistochemical....pdf.txtExtracted texttext/plain30446https://www.arca.fiocruz.br/bitstream/icict/8926/3/Boas%20DSV%20Immunohistochemical....pdf.txt5fde344c50f891ff3168637a1a659785MD53icict/89262023-03-15 14:33:15.307oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:15Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Immunohistochemical detection of Ki-67 is not associated with tumor-infiltrating macrophages and cyclooxygenase-2 in oral squamous cell carcinoma.
title Immunohistochemical detection of Ki-67 is not associated with tumor-infiltrating macrophages and cyclooxygenase-2 in oral squamous cell carcinoma.
spellingShingle Immunohistochemical detection of Ki-67 is not associated with tumor-infiltrating macrophages and cyclooxygenase-2 in oral squamous cell carcinoma.
Bôas, Deise Souza Vilas
Cyclooxygenase-2
Imunohistochemistry
Ki-67
Macrophages
Squamous cell carcinoma
Carcinoma de Células Escamosas/patologia
Ciclo-Oxigenase 2/análise
Antígeno Ki-67/análise
Macrófagos/patologia
Neoplasias Bucais/patologia
Antígenos CD/análise
Antígenos de Diferenciação Mielomonocítica/análise
Carcinoma in Situ/enzimologia
Carcinoma de Células Escamosas/enzimologia
Núcleo Celular/ultraestrutura
Corantes/uso diagnóstico
Citoplasma/ultraestrutura
Células Epiteliais/enzimologia
Humanos
Imuno-Histoquímica
Neoplasias Bucais/enzimologia
Invasividade Neoplásica
Marcadores Biológicos de Tumor/análise
title_short Immunohistochemical detection of Ki-67 is not associated with tumor-infiltrating macrophages and cyclooxygenase-2 in oral squamous cell carcinoma.
title_full Immunohistochemical detection of Ki-67 is not associated with tumor-infiltrating macrophages and cyclooxygenase-2 in oral squamous cell carcinoma.
title_fullStr Immunohistochemical detection of Ki-67 is not associated with tumor-infiltrating macrophages and cyclooxygenase-2 in oral squamous cell carcinoma.
title_full_unstemmed Immunohistochemical detection of Ki-67 is not associated with tumor-infiltrating macrophages and cyclooxygenase-2 in oral squamous cell carcinoma.
title_sort Immunohistochemical detection of Ki-67 is not associated with tumor-infiltrating macrophages and cyclooxygenase-2 in oral squamous cell carcinoma.
author Bôas, Deise Souza Vilas
author_facet Bôas, Deise Souza Vilas
Takiya, Christina Maeda
Sampaio, Tatiana Lobo Coelho
Ribeiro, Leonardo Campos Monção
Ramos, Eduardo Antônio Gonçalves
Cabral, Márcia Grillo
Santos, Jean Nunes dos
author_role author
author2 Takiya, Christina Maeda
Sampaio, Tatiana Lobo Coelho
Ribeiro, Leonardo Campos Monção
Ramos, Eduardo Antônio Gonçalves
Cabral, Márcia Grillo
Santos, Jean Nunes dos
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bôas, Deise Souza Vilas
Takiya, Christina Maeda
Sampaio, Tatiana Lobo Coelho
Ribeiro, Leonardo Campos Monção
Ramos, Eduardo Antônio Gonçalves
Cabral, Márcia Grillo
Santos, Jean Nunes dos
dc.subject.en.pt_BR.fl_str_mv Cyclooxygenase-2
Imunohistochemistry
Ki-67
Macrophages
Squamous cell carcinoma
topic Cyclooxygenase-2
Imunohistochemistry
Ki-67
Macrophages
Squamous cell carcinoma
Carcinoma de Células Escamosas/patologia
Ciclo-Oxigenase 2/análise
Antígeno Ki-67/análise
Macrófagos/patologia
Neoplasias Bucais/patologia
Antígenos CD/análise
Antígenos de Diferenciação Mielomonocítica/análise
Carcinoma in Situ/enzimologia
Carcinoma de Células Escamosas/enzimologia
Núcleo Celular/ultraestrutura
Corantes/uso diagnóstico
Citoplasma/ultraestrutura
Células Epiteliais/enzimologia
Humanos
Imuno-Histoquímica
Neoplasias Bucais/enzimologia
Invasividade Neoplásica
Marcadores Biológicos de Tumor/análise
dc.subject.decs.pt_BR.fl_str_mv Carcinoma de Células Escamosas/patologia
Ciclo-Oxigenase 2/análise
Antígeno Ki-67/análise
Macrófagos/patologia
Neoplasias Bucais/patologia
Antígenos CD/análise
Antígenos de Diferenciação Mielomonocítica/análise
Carcinoma in Situ/enzimologia
Carcinoma de Células Escamosas/enzimologia
Núcleo Celular/ultraestrutura
Corantes/uso diagnóstico
Citoplasma/ultraestrutura
Células Epiteliais/enzimologia
Humanos
Imuno-Histoquímica
Neoplasias Bucais/enzimologia
Invasividade Neoplásica
Marcadores Biológicos de Tumor/análise
description Federal University of Bahia. Institute of Health Sciences. Department of Bio-morphology. Salvador, BA, Brasil / Federal University of Rio de Janeiro. Institute of Biomedical Sciences. Department of Histology and Embryology. Rio de Janeiro, RJ, Brasil
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2014-11-20T18:30:16Z
dc.date.available.fl_str_mv 2014-11-20T18:30:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BOAS, D. S. V. et al. Immunohistochemical detection of Ki-67 is not associated with tumor-infiltrating macrophages and cyclooxygenase-2 in oral squamous cell carcinoma. Journal of Oral Pathology & Medicine, v. 39, 7, p. 565-570, 2010.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8926
dc.identifier.issn.none.fl_str_mv 1600-0714
dc.identifier.doi.none.fl_str_mv 10.1111/j.1600-0714.2010.00883.x
identifier_str_mv BOAS, D. S. V. et al. Immunohistochemical detection of Ki-67 is not associated with tumor-infiltrating macrophages and cyclooxygenase-2 in oral squamous cell carcinoma. Journal of Oral Pathology & Medicine, v. 39, 7, p. 565-570, 2010.
1600-0714
10.1111/j.1600-0714.2010.00883.x
url https://www.arca.fiocruz.br/handle/icict/8926
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv John Wiley & Sons Ltd
publisher.none.fl_str_mv John Wiley & Sons Ltd
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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