Cryptococcus neoformans glucuronoxylomannan and sterylglucoside are required for host protection in an animal vaccination model
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/34169 |
Resumo: | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil. / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil. / Department of Molecular Genetics and Microbiology. Stony Brook University. Stony Brook, New York, USA. |
| id |
CRUZ_7d861694f7400ca9fcbcf7b2f7a57e1e |
|---|---|
| oai_identifier_str |
oai:www.arca.fiocruz.br:icict/34169 |
| network_acronym_str |
CRUZ |
| network_name_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| repository_id_str |
2135 |
| spelling |
Colombo, Ana CarolineRella, AntonellaNormile, TylerJoffe, Luna S.Tavares, Patricia M.Araújo, Glauber Ribeiro de SousaFrases, SusanaOrner, Erika P.Farnoud, Amir M.Fries, Bettina C.Sheridan, BrianNimrichter, LeonardoRodrigues, Marcio LourençoDel Poeta, Maurizio2019-07-16T18:10:16Z2019-07-16T18:10:16Z2019COLOMBO, Ana Caroline et al. Cryptococcus neoformans Glucuronoxylomannan and Sterylglucoside are required for host protection in an animal vaccination model. mBio, v. 10, n. 2, p. 1–22, 2019.2150-7511https://www.arca.fiocruz.br/handle/icict/3416910.1128/mBio.02909-182150-7511porAmerican Society for MicrobiologyEstéril GlucosidaseGlucuronoxilomananaCapsulesExtracellular VesiclesMycosesfungal infectionGlycolipidsPolysaccharidesVaccinesMeningitis, CryptococcalMicosisGlucolípidosPolisacáridosVacunasMeningitis CriptocócicaCryptococcus neoformansCápsulasVesículas ExtracelularesMicosesGlicolipídeosPolissacarídeosVacinasMeningite CriptocócicaCryptococcus neoformans glucuronoxylomannan and sterylglucoside are required for host protection in an animal vaccination modelinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil. / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil. / Department of Molecular Genetics and Microbiology. Stony Brook University. Stony Brook, New York, USA.Department of Molecular Genetics and Microbiology. Stony Brook University. Stony Brook, New York, USA.Department of Molecular Genetics and Microbiology. Stony Brook University. Stony Brook, New York, USA.Department of Molecular Genetics and Microbiology. Stony Brook University. Stony Brook, New York, USA. / Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Department of Molecular Genetics and Microbiology. Stony Brook University. Stony Brook, New York, USA.Department of Molecular Genetics and Microbiology. Stony Brook University. Stony Brook, New York, USA. / Chemical and Biomolecular Engineering Department. Ohio University. Athens, Ohio, USA.Department of Molecular Genetics and Microbiology. Stony Brook University. Stony Brook, New York, USA. / Department of Medicine. Division of Infectious Diseases. Stony Brook University. Stony Brook, New York, USA.Department of Molecular Genetics and Microbiology. Stony Brook University. Stony Brook, New York, USA.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Department of Molecular Genetics and Microbiology. Stony Brook University. Stony Brook, New York, USA. / Department of Medicine. Division of Infectious Diseases. Stony Brook University. Stony Brook, New York, USA. / Veterans Administration Medical Center. Northport, New York, USA.Cryptococcus neoformans is an encapsulated fungal pathogen that causes meningoencephalitis. There are no prophylactic tools for cryptococcosis. Previously, our group showed that a C. neoformans mutant lacking the gene encoding sterylglucosidase (Δsgl1) induced protection in both immunocompetent and immunocompromised murine models of cryptococcosis. Since sterylglucosidase catalyzes degradation of sterylglucosides (SGs), accumulation of this glycolipid could be responsible for protective immunity. In this study, we analyzed whether the activity of SGs is sufficient for the protective effect induced by the Δsgl1 strain. We observed that the accumulation of SGs impacted several properties of the main polysaccharide that composes the fungal capsule, glucuronoxylomannan (GXM). We therefore used genetic manipulation to delete the SGL1 gene in the acapsular mutant Δcap59 to generate a double mutant (strain Δcap59/Δsgl1) that was shown to be nonpathogenic and cleared from the lung of mice within 7 days post-intranasal infection. The inflammatory immune response triggered by the Δcap59/Δsgl1 mutant in the lung differed from the response seen with the other strains. The double mutant did not induce protection in a vaccination model, suggesting that SG-related protection requires the main capsular polysaccharide. Finally, GXM-containing extracellular vesicles (EVs) enriched in SGs delayed the acute lethality of Galleria mellonella against C. neoformans infection. These studies highlighted a key role for GXM and SGs in inducing protection against a secondary cryptococcal infection, and, since EVs notoriously contain GXM, these results suggest the potential use of Δsgl1 EVs as a vaccination strategy for cryptococcosis. Important information: The number of deaths from cryptococcal meningitis is around 180,000 per year. The disease is the second leading cause of mortality among individuals with AIDS. Antifungal treatment is costly and associated with adverse effects and resistance, evidencing the urgency of development of both therapeutic and prophylactic tools. Here we demonstrate the key roles of polysaccharide- and glycolipid-containing structures in a vaccination model to prevent cryptococcosis.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/34169/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALmBio-2019-Colombo.pdfmBio-2019-Colombo.pdfapplication/pdf4851028https://www.arca.fiocruz.br/bitstream/icict/34169/2/mBio-2019-Colombo.pdf428104c235477c660a57208995f3835fMD52TEXTmBio-2019-Colombo.pdf.txtmBio-2019-Colombo.pdf.txtExtracted texttext/plain90269https://www.arca.fiocruz.br/bitstream/icict/34169/3/mBio-2019-Colombo.pdf.txt8afae25902b4d67b2244a160f7a7b6e2MD53icict/341692023-01-26 15:00:00.916oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-01-26T18:00Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Cryptococcus neoformans glucuronoxylomannan and sterylglucoside are required for host protection in an animal vaccination model |
| title |
Cryptococcus neoformans glucuronoxylomannan and sterylglucoside are required for host protection in an animal vaccination model |
| spellingShingle |
Cryptococcus neoformans glucuronoxylomannan and sterylglucoside are required for host protection in an animal vaccination model Colombo, Ana Caroline Estéril Glucosidase Glucuronoxilomanana Capsules Extracellular Vesicles Mycoses fungal infection Glycolipids Polysaccharides Vaccines Meningitis, Cryptococcal Micosis Glucolípidos Polisacáridos Vacunas Meningitis Criptocócica Cryptococcus neoformans Cápsulas Vesículas Extracelulares Micoses Glicolipídeos Polissacarídeos Vacinas Meningite Criptocócica |
| title_short |
Cryptococcus neoformans glucuronoxylomannan and sterylglucoside are required for host protection in an animal vaccination model |
| title_full |
Cryptococcus neoformans glucuronoxylomannan and sterylglucoside are required for host protection in an animal vaccination model |
| title_fullStr |
Cryptococcus neoformans glucuronoxylomannan and sterylglucoside are required for host protection in an animal vaccination model |
| title_full_unstemmed |
Cryptococcus neoformans glucuronoxylomannan and sterylglucoside are required for host protection in an animal vaccination model |
| title_sort |
Cryptococcus neoformans glucuronoxylomannan and sterylglucoside are required for host protection in an animal vaccination model |
| author |
Colombo, Ana Caroline |
| author_facet |
Colombo, Ana Caroline Rella, Antonella Normile, Tyler Joffe, Luna S. Tavares, Patricia M. Araújo, Glauber Ribeiro de Sousa Frases, Susana Orner, Erika P. Farnoud, Amir M. Fries, Bettina C. Sheridan, Brian Nimrichter, Leonardo Rodrigues, Marcio Lourenço Del Poeta, Maurizio |
| author_role |
author |
| author2 |
Rella, Antonella Normile, Tyler Joffe, Luna S. Tavares, Patricia M. Araújo, Glauber Ribeiro de Sousa Frases, Susana Orner, Erika P. Farnoud, Amir M. Fries, Bettina C. Sheridan, Brian Nimrichter, Leonardo Rodrigues, Marcio Lourenço Del Poeta, Maurizio |
| author2_role |
author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Colombo, Ana Caroline Rella, Antonella Normile, Tyler Joffe, Luna S. Tavares, Patricia M. Araújo, Glauber Ribeiro de Sousa Frases, Susana Orner, Erika P. Farnoud, Amir M. Fries, Bettina C. Sheridan, Brian Nimrichter, Leonardo Rodrigues, Marcio Lourenço Del Poeta, Maurizio |
| dc.subject.other.pt_BR.fl_str_mv |
Estéril Glucosidase Glucuronoxilomanana |
| topic |
Estéril Glucosidase Glucuronoxilomanana Capsules Extracellular Vesicles Mycoses fungal infection Glycolipids Polysaccharides Vaccines Meningitis, Cryptococcal Micosis Glucolípidos Polisacáridos Vacunas Meningitis Criptocócica Cryptococcus neoformans Cápsulas Vesículas Extracelulares Micoses Glicolipídeos Polissacarídeos Vacinas Meningite Criptocócica |
| dc.subject.en.pt_BR.fl_str_mv |
Capsules Extracellular Vesicles Mycoses fungal infection Glycolipids Polysaccharides Vaccines Meningitis, Cryptococcal |
| dc.subject.es.pt_BR.fl_str_mv |
Micosis Glucolípidos Polisacáridos Vacunas Meningitis Criptocócica |
| dc.subject.decs.pt_BR.fl_str_mv |
Cryptococcus neoformans Cápsulas Vesículas Extracelulares Micoses Glicolipídeos Polissacarídeos Vacinas Meningite Criptocócica |
| description |
Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil. / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil. / Department of Molecular Genetics and Microbiology. Stony Brook University. Stony Brook, New York, USA. |
| publishDate |
2019 |
| dc.date.accessioned.fl_str_mv |
2019-07-16T18:10:16Z |
| dc.date.available.fl_str_mv |
2019-07-16T18:10:16Z |
| dc.date.issued.fl_str_mv |
2019 |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.citation.fl_str_mv |
COLOMBO, Ana Caroline et al. Cryptococcus neoformans Glucuronoxylomannan and Sterylglucoside are required for host protection in an animal vaccination model. mBio, v. 10, n. 2, p. 1–22, 2019. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/34169 |
| dc.identifier.issn.pt_BR.fl_str_mv |
2150-7511 |
| dc.identifier.doi.none.fl_str_mv |
10.1128/mBio.02909-18 |
| dc.identifier.eissn.none.fl_str_mv |
2150-7511 |
| identifier_str_mv |
COLOMBO, Ana Caroline et al. Cryptococcus neoformans Glucuronoxylomannan and Sterylglucoside are required for host protection in an animal vaccination model. mBio, v. 10, n. 2, p. 1–22, 2019. 2150-7511 10.1128/mBio.02909-18 |
| url |
https://www.arca.fiocruz.br/handle/icict/34169 |
| dc.language.iso.fl_str_mv |
por |
| language |
por |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
American Society for Microbiology |
| publisher.none.fl_str_mv |
American Society for Microbiology |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
| instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
| instacron_str |
FIOCRUZ |
| institution |
FIOCRUZ |
| reponame_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| collection |
Repositório Institucional da FIOCRUZ (ARCA) |
| bitstream.url.fl_str_mv |
https://www.arca.fiocruz.br/bitstream/icict/34169/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/34169/2/mBio-2019-Colombo.pdf https://www.arca.fiocruz.br/bitstream/icict/34169/3/mBio-2019-Colombo.pdf.txt |
| bitstream.checksum.fl_str_mv |
783568c2893d2e25a99990b126be1772 428104c235477c660a57208995f3835f 8afae25902b4d67b2244a160f7a7b6e2 |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ) |
| repository.mail.fl_str_mv |
repositorio.arca@fiocruz.br |
| _version_ |
1813009129778708480 |