Pathogen-induced proapoptotic phenotype and high CD95 (Fas) expression accompany a suboptimal CD8+ T-Cell response: reversal by adenoviral vaccine
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2012 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/7935 |
Resumo: | Universidade Federal de São Paulo. Escola Paulista de Medicina. Centro de Terapia Celular e Molecular. São Paulo, SP, Brazil/Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil |
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Vasconcelos, José RonnieAraujo, Adriano Fernando da SilvaDominguez, Mariana RibeiroErsching, JonatanBargieri, Bruna Cunha de AlencarBortoluci, Karina RamalhoRodrigues, Maurício MartinsBruna-Romero, OscarMachado, Alexandre de Magalhaes VieiraGazzinelli, Ricardo TostesMendes, João Gustavo Pessini AmaranteLopes, Marcela de Freitas2014-07-11T18:36:23Z2014-07-11T18:36:23Z2012VASCONCELOS, José Ronnie et al. Pathogen-induced proapoptotic phenotype and high CD95 (Fas) expression accompany a suboptimal CD8+ T-Cell response: reversal by adenoviral vaccine. Plos Pathogens. 2012, vol.8, pp. e1002699.1553-7366https://www.arca.fiocruz.br/handle/icict/7935engPublic Library of SciencePathogen-induced proapoptotic phenotype and high CD95 (Fas) expression accompany a suboptimal CD8+ T-Cell response: reversal by adenoviral vaccineinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de São Paulo. Escola Paulista de Medicina. Centro de Terapia Celular e Molecular. São Paulo, SP, Brazil/Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, BrazilUniversidade Federal de São Paulo. Escola Paulista de Medicina. Centro de Terapia Celular e Molecular. São Paulo, SP, Brazil/Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, BrazilUniversidade Federal de São Paulo. Escola Paulista de Medicina. Centro de Terapia Celular e Molecular. São Paulo, SP, Brazil/Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, BrazilUniversidade Federal de São Paulo. Escola Paulista de Medicina. Centro de Terapia Celular e Molecular. São Paulo, SP, Brazil/Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, BrazilUniversidade Federal de São Paulo. Escola Paulista de Medicina. Centro de Terapia Celular e Molecular. São Paulo, SP, Brazil/Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, BrazilUniversidade Federal de São Paulo. Escola Paulista de Medicina. Centro de Terapia Celular e Molecular. São Paulo, SP, Brazil/Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Ciências Biológicas. Diadema, SP, BrazilUniversidade Federal de São Paulo. Escola Paulista de Medicina. Centro de Terapia Celular e Molecular. São Paulo, SP, Brazil/Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil/University of Massachusetts. Medical School. Department of Medicine. Division of Infectious Disease and Immunology. Worcester, MA, United States of AmericaUniversidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Imunologia. São Paulo, SP, BrazilUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, BrazilMHC class Ia-restricted CD8+ T cells are important mediators of the adaptive immune response against infections caused by intracellular microorganisms. Whereas antigen-specific effector CD8+ T cells can clear infection caused by intracellular pathogens, in some circumstances, the immune response is suboptimal and the microorganisms survive, causing host death or chronic infection. Here, we explored the cellular and molecular mechanisms that could explain why CD8+ T cell-mediated immunity during infection with the human protozoan parasite Trypanosoma cruzi is not optimal. For that purpose, we compared the CD8+ T-cell mediated immune responses in mice infected with T. cruzi or vaccinated with a recombinant adenovirus expressing an immunodominant parasite antigen. Several functional and phenotypic characteristics of specific CD8+ T cells overlapped. Among few exceptions was an accelerated expansion of the immune response in adenoviral vaccinated mice when compared to infected ones. Also, there was an upregulated expression of the apoptotic-signaling receptor CD95 on the surface of specific T cells from infected mice, which was not observed in the case of adenoviral-vaccinated mice. Most importantly, adenoviral vaccine provided at the time of infection significantly reduced the upregulation of CD95 expression and the proapoptotic phenotype of pathogen-specific CD8+ cells expanded during infection. In parallel, infected adenovirus-vaccinated mice had a stronger CD8 T-cell mediated immune response and survived an otherwise lethal infection. We concluded that a suboptimal CD8+ T-cell response is associated with an upregulation of CD95 expression and a proapoptotic phenotype. Both can be blocked by adenoviral vaccination.Parasitic diseasesProtozoan infectionsT cellsTrypanosoma cruziinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALPathogen-induced proapoptotic phenotype and high CD95 (Fas) expression accompany a suboptimal CD8+ T-Cell response.pdfPathogen-induced proapoptotic phenotype and high CD95 (Fas) expression accompany a suboptimal CD8+ T-Cell response.pdfapplication/pdf4762025https://www.arca.fiocruz.br/bitstream/icict/7935/1/Pathogen-induced%20proapoptotic%20phenotype%20and%20high%20CD95%20%28Fas%29%20expression%20accompany%20a%20suboptimal%20CD8%2b%20T-Cell%20response.pdf91b228e6248dedf806f22067b2b264e5MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7935/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTPathogen-induced proapoptotic phenotype and high CD95 (Fas) expression accompany a suboptimal CD8+ T-Cell response.pdf.txtPathogen-induced proapoptotic phenotype and high CD95 (Fas) expression accompany a suboptimal CD8+ T-Cell response.pdf.txtExtracted texttext/plain16https://www.arca.fiocruz.br/bitstream/icict/7935/3/Pathogen-induced%20proapoptotic%20phenotype%20and%20high%20CD95%20%28Fas%29%20expression%20accompany%20a%20suboptimal%20CD8%2b%20T-Cell%20response.pdf.txt24d68299ae13852334a122ea657bbaaaMD53icict/79352018-04-05 23:45:29.41oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-06T02:45:29Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Pathogen-induced proapoptotic phenotype and high CD95 (Fas) expression accompany a suboptimal CD8+ T-Cell response: reversal by adenoviral vaccine |
| title |
Pathogen-induced proapoptotic phenotype and high CD95 (Fas) expression accompany a suboptimal CD8+ T-Cell response: reversal by adenoviral vaccine |
| spellingShingle |
Pathogen-induced proapoptotic phenotype and high CD95 (Fas) expression accompany a suboptimal CD8+ T-Cell response: reversal by adenoviral vaccine Vasconcelos, José Ronnie Parasitic diseases Protozoan infections T cells Trypanosoma cruzi |
| title_short |
Pathogen-induced proapoptotic phenotype and high CD95 (Fas) expression accompany a suboptimal CD8+ T-Cell response: reversal by adenoviral vaccine |
| title_full |
Pathogen-induced proapoptotic phenotype and high CD95 (Fas) expression accompany a suboptimal CD8+ T-Cell response: reversal by adenoviral vaccine |
| title_fullStr |
Pathogen-induced proapoptotic phenotype and high CD95 (Fas) expression accompany a suboptimal CD8+ T-Cell response: reversal by adenoviral vaccine |
| title_full_unstemmed |
Pathogen-induced proapoptotic phenotype and high CD95 (Fas) expression accompany a suboptimal CD8+ T-Cell response: reversal by adenoviral vaccine |
| title_sort |
Pathogen-induced proapoptotic phenotype and high CD95 (Fas) expression accompany a suboptimal CD8+ T-Cell response: reversal by adenoviral vaccine |
| author |
Vasconcelos, José Ronnie |
| author_facet |
Vasconcelos, José Ronnie Araujo, Adriano Fernando da Silva Dominguez, Mariana Ribeiro Ersching, Jonatan Bargieri, Bruna Cunha de Alencar Bortoluci, Karina Ramalho Rodrigues, Maurício Martins Bruna-Romero, Oscar Machado, Alexandre de Magalhaes Vieira Gazzinelli, Ricardo Tostes Mendes, João Gustavo Pessini Amarante Lopes, Marcela de Freitas |
| author_role |
author |
| author2 |
Araujo, Adriano Fernando da Silva Dominguez, Mariana Ribeiro Ersching, Jonatan Bargieri, Bruna Cunha de Alencar Bortoluci, Karina Ramalho Rodrigues, Maurício Martins Bruna-Romero, Oscar Machado, Alexandre de Magalhaes Vieira Gazzinelli, Ricardo Tostes Mendes, João Gustavo Pessini Amarante Lopes, Marcela de Freitas |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Vasconcelos, José Ronnie Araujo, Adriano Fernando da Silva Dominguez, Mariana Ribeiro Ersching, Jonatan Bargieri, Bruna Cunha de Alencar Bortoluci, Karina Ramalho Rodrigues, Maurício Martins Bruna-Romero, Oscar Machado, Alexandre de Magalhaes Vieira Gazzinelli, Ricardo Tostes Mendes, João Gustavo Pessini Amarante Lopes, Marcela de Freitas |
| dc.subject.en.pt_BR.fl_str_mv |
Parasitic diseases Protozoan infections T cells Trypanosoma cruzi |
| topic |
Parasitic diseases Protozoan infections T cells Trypanosoma cruzi |
| description |
Universidade Federal de São Paulo. Escola Paulista de Medicina. Centro de Terapia Celular e Molecular. São Paulo, SP, Brazil/Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil |
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2012 |
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2012 |
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2014-07-11T18:36:23Z |
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2014-07-11T18:36:23Z |
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info:eu-repo/semantics/article |
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article |
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VASCONCELOS, José Ronnie et al. Pathogen-induced proapoptotic phenotype and high CD95 (Fas) expression accompany a suboptimal CD8+ T-Cell response: reversal by adenoviral vaccine. Plos Pathogens. 2012, vol.8, pp. e1002699. |
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https://www.arca.fiocruz.br/handle/icict/7935 |
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1553-7366 |
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VASCONCELOS, José Ronnie et al. Pathogen-induced proapoptotic phenotype and high CD95 (Fas) expression accompany a suboptimal CD8+ T-Cell response: reversal by adenoviral vaccine. Plos Pathogens. 2012, vol.8, pp. e1002699. 1553-7366 |
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