Preparation and characterization of a bacterial cellulose/silk fibroin sponge scaffold for tissue regeneration.

Detalhes bibliográficos
Autor(a) principal: Barud, Hélida Gomes de Oliveira
Data de Publicação: 2015
Outros Autores: Barud, Hernane da Silva, Cavicchioli, Maurício, Amaral, Thais Silva do, Oliveira Junior, Osmir Batista de, Santos, Diego Moura, Petersen, Antonio Luis de Oliveira Almeida, Celes, Fabiana, Borges, Valeria de Matos, Oliveira, Camila Indiani de, Oliveira, Pollyanna Francielli de, Furtado, Ricardo Andrade, Tavares, Denise Crispim, Ribeiro, Sidney Jose Lima
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/11166
Resumo: São Paulo State University. UNESP. School of Dentistry. Araraquara, SP, Brasil
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spelling Barud, Hélida Gomes de OliveiraBarud, Hernane da SilvaCavicchioli, MaurícioAmaral, Thais Silva doOliveira Junior, Osmir Batista deSantos, Diego MouraPetersen, Antonio Luis de Oliveira AlmeidaCeles, FabianaBorges, Valeria de MatosOliveira, Camila Indiani deOliveira, Pollyanna Francielli deFurtado, Ricardo AndradeTavares, Denise CrispimRibeiro, Sidney Jose Lima2015-07-08T13:47:12Z2015-07-08T13:47:12Z2015BARUD, H. G. O. et al. Preparation and characterization of a bacterial cellulose/silk fibroin sponge scaffold for tissue regeneration. Carbohydrate Polymers, v. 128, p. 41-51, 20151879-1344https://www.arca.fiocruz.br/handle/icict/111661016/j.carbpol.2015.04.07engElsevierPreparation and characterization of a bacterial cellulose/silk fibroin sponge scaffold for tissue regeneration.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSão Paulo State University. UNESP. School of Dentistry. Araraquara, SP, BrasilSão Paulo State University. UNESP. Institute of Chemistry. Araraquara, SP, Brasil / Centro Universitário de Araraquara. Laboratório de Química Medicinal e Medicina Regenerativa. Araraquara, SP, BrasilSão Paulo State University. UNESP. Institute of Chemistry. Araraquara, SP, BrasilSão Paulo State University. UNESP. Institute of Chemistry. Araraquara, SP, BrasilSão Paulo State University. UNESP. School of Dentistry. Araraquara, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversity of França. Franca, SP, BrasilUniversity of França. Franca, SP, BrasilUniversity of França. Franca, SP, BrasilSão Paulo State University. UNESP. Institute of Chemistry. Araraquara, SP, BrasilBacterial cellulose (BC) and silk fibroin (SF) are natural biopolymers successfully applied in tissue engineering and biomedical fields. In this work nanocomposites based on BC and SF were prepared and characterized by scanning electron microscopy (SEM), infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and thermogravimetric analysis (TGA). In addition, the investigation of cytocompatibility was done by MTT, XTT and Trypan Blue dye technique. Cellular adhesion and proliferation were detected additionally. The evaluation of genotoxicity was realized by micronucleus assay. In vitro tests showed that the material is non-cytotoxic or genotoxic. SEM images revealed a greater number of cells attached at the BC/SF:50% scaffold surface than the pure BC one, suggesting that the presence of fibroin improved cell attachment. This could be related to the SF amino acid sequence that acts as cell receptors facilitating cell adhesion and growth. Consequently, BC/SF:50% scaffolds configured an excellent option in bioengineering depicting its potential for tissue regeneration and cultivation of cells on nanocompositesBacterial celluloseSilk fibroinBiocompatible materialsNanocompositesScaffoldTissue engineeringinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALBarud HGO Preparation....pdfBarud HGO Preparation....pdfapplication/pdf2210539https://www.arca.fiocruz.br/bitstream/icict/11166/2/Barud%20HGO%20Preparation....pdfc2350d250f300dc3ba86977b3dd702daMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82354https://www.arca.fiocruz.br/bitstream/icict/11166/3/license.txt8b4c200b4e10021c5683c6ccaba07169MD53TEXTBarud HGO Preparation....pdf.txtBarud HGO Preparation....pdf.txtExtracted texttext/plain64398https://www.arca.fiocruz.br/bitstream/icict/11166/4/Barud%20HGO%20Preparation....pdf.txtc473fc19906e04acb056736c365a13adMD54icict/111662023-03-15 14:33:09.124oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:09Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Preparation and characterization of a bacterial cellulose/silk fibroin sponge scaffold for tissue regeneration.
title Preparation and characterization of a bacterial cellulose/silk fibroin sponge scaffold for tissue regeneration.
spellingShingle Preparation and characterization of a bacterial cellulose/silk fibroin sponge scaffold for tissue regeneration.
Barud, Hélida Gomes de Oliveira
Bacterial cellulose
Silk fibroin
Biocompatible materials
Nanocomposites
Scaffold
Tissue engineering
title_short Preparation and characterization of a bacterial cellulose/silk fibroin sponge scaffold for tissue regeneration.
title_full Preparation and characterization of a bacterial cellulose/silk fibroin sponge scaffold for tissue regeneration.
title_fullStr Preparation and characterization of a bacterial cellulose/silk fibroin sponge scaffold for tissue regeneration.
title_full_unstemmed Preparation and characterization of a bacterial cellulose/silk fibroin sponge scaffold for tissue regeneration.
title_sort Preparation and characterization of a bacterial cellulose/silk fibroin sponge scaffold for tissue regeneration.
author Barud, Hélida Gomes de Oliveira
author_facet Barud, Hélida Gomes de Oliveira
Barud, Hernane da Silva
Cavicchioli, Maurício
Amaral, Thais Silva do
Oliveira Junior, Osmir Batista de
Santos, Diego Moura
Petersen, Antonio Luis de Oliveira Almeida
Celes, Fabiana
Borges, Valeria de Matos
Oliveira, Camila Indiani de
Oliveira, Pollyanna Francielli de
Furtado, Ricardo Andrade
Tavares, Denise Crispim
Ribeiro, Sidney Jose Lima
author_role author
author2 Barud, Hernane da Silva
Cavicchioli, Maurício
Amaral, Thais Silva do
Oliveira Junior, Osmir Batista de
Santos, Diego Moura
Petersen, Antonio Luis de Oliveira Almeida
Celes, Fabiana
Borges, Valeria de Matos
Oliveira, Camila Indiani de
Oliveira, Pollyanna Francielli de
Furtado, Ricardo Andrade
Tavares, Denise Crispim
Ribeiro, Sidney Jose Lima
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Barud, Hélida Gomes de Oliveira
Barud, Hernane da Silva
Cavicchioli, Maurício
Amaral, Thais Silva do
Oliveira Junior, Osmir Batista de
Santos, Diego Moura
Petersen, Antonio Luis de Oliveira Almeida
Celes, Fabiana
Borges, Valeria de Matos
Oliveira, Camila Indiani de
Oliveira, Pollyanna Francielli de
Furtado, Ricardo Andrade
Tavares, Denise Crispim
Ribeiro, Sidney Jose Lima
dc.subject.en.pt_BR.fl_str_mv Bacterial cellulose
Silk fibroin
Biocompatible materials
Nanocomposites
Scaffold
Tissue engineering
topic Bacterial cellulose
Silk fibroin
Biocompatible materials
Nanocomposites
Scaffold
Tissue engineering
description São Paulo State University. UNESP. School of Dentistry. Araraquara, SP, Brasil
publishDate 2015
dc.date.accessioned.fl_str_mv 2015-07-08T13:47:12Z
dc.date.available.fl_str_mv 2015-07-08T13:47:12Z
dc.date.issued.fl_str_mv 2015
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BARUD, H. G. O. et al. Preparation and characterization of a bacterial cellulose/silk fibroin sponge scaffold for tissue regeneration. Carbohydrate Polymers, v. 128, p. 41-51, 2015
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/11166
dc.identifier.issn.none.fl_str_mv 1879-1344
dc.identifier.doi.none.fl_str_mv 1016/j.carbpol.2015.04.07
identifier_str_mv BARUD, H. G. O. et al. Preparation and characterization of a bacterial cellulose/silk fibroin sponge scaffold for tissue regeneration. Carbohydrate Polymers, v. 128, p. 41-51, 2015
1879-1344
1016/j.carbpol.2015.04.07
url https://www.arca.fiocruz.br/handle/icict/11166
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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