Performance of Treponema pallidum recombinant proteins in the serological diagnosis of syphilis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/42518 |
Resumo: | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. |
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Silva, Ângelo Antônio OliveiraVasconcelos, Larissa de Carvalho MedradoFoti, LeonardoLeony, Leonardo MaiaDaltro, Ramona TavaresLeitolis, AmandaLima, Fernanda Washington de MendonçaKrieger, Marco AurélioZanchin, Nilson Ivo ToninSantos, Fred Luciano Neves2020-08-03T20:12:14Z2020-08-03T20:12:14Z2020SILVA, Ângelo Antônio Oliveira et al. Performance of Treponema pallidum recombinant proteins in the serological diagnosis of syphilis. Plos One, v. 11, n. 6, p. 1-21, 2020.1932-6203https://www.arca.fiocruz.br/handle/icict/4251810.1371/journal.pone.02340431932-6203porPublic Library of ScienceTreponema pallidumSyphilis SerodiagnosisSyphilisRecombinant ProteinsDiagnosisAntigenic VariationSerodiagnóstico de la SífilisVariación AntigénicaSorodiagnóstico da SífilisSífilisProteínas RecombinantesDiagnósticoVariação AntigênicaPerformance of Treponema pallidum recombinant proteins in the serological diagnosis of syphilisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal do Paraná. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal do Paraná. Curitiba, PR, Brasil.Universidade Federal da Bahia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Syphilis serodiagnosis is challenging because distinct clinical forms of the infection may influence serological performance and discordant results between tests make clinical decisions difficult. Several recombinant Treponema pallidum-proteins have already been tested for syphilis diagnosis and they are critical to achieve high accuracy in serological testing. Our aim was to assess the varied from performance of T. pallidum-recombinant proteins TmpA, TpN17 and TpN47 for syphilis serodiagnosis. The proteins were evaluated using sera of 338 T. pallidum-negative, 173 T. pallidum-positive individuals and 209 sera from individuals infected with unrelated diseases. The diagnostic potential was validated by analysis of ROC curves. In the liquid microarray analyses, the ROC curve varied from 99.0% for TmpA and TpN17 to 100% for TpN47. The sensitivity score yielded values of up to 90% for TpN17, 100% for TpN47 and 80.0% for TmpA. The lowest and highest specificity values were presented by TpN47 (91.9%) and TmpA antigens (100%), respectively. TpN47 showed the highest accuracy score (95.5%) among all the recombinant proteins assayed. For the ELISA, the ROC curve was 97.2%, 91.8% and 81.6% for TpN17, TmpA and TpN47, respectively. TpN17 and TmpA yielded a sensitivity of 69.9%, while TpN47 obtained a value of 53.8%. Specificity was almost 100% for all three proteins. No cross-reaction was observed for TpN17 in the serum samples from non-bacterial infections. Regarding leptospirosis-positive samples, cross-reactivity score was varied from 8.6 to 34.6%. This is most probably due to conservation of the epitopes in these proteins across bacteria. The use of recombinant proteins in immunoassays for syphilis diagnosis was showed provide greater reliability to results of the treponemal assays. Despite the low sensitivity, the proteins showed high diagnostic capacity due to the AUC values found. However, an improvement in sensitivity could be achieved when antigenic mixtures are evaluated.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/42518/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALjournal.pone.02340ok.pdfjournal.pone.02340ok.pdfapplication/pdf3128888https://www.arca.fiocruz.br/bitstream/icict/42518/2/journal.pone.02340ok.pdfaf62ae40e219280a837131cd311644feMD52TEXTjournal.pone.02340ok.pdf.txtjournal.pone.02340ok.pdf.txtExtracted texttext/plain65110https://www.arca.fiocruz.br/bitstream/icict/42518/3/journal.pone.02340ok.pdf.txt5fa61113178a9a61b9e5bdcb002b5359MD53icict/425182020-09-29 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| dc.title.pt_BR.fl_str_mv |
Performance of Treponema pallidum recombinant proteins in the serological diagnosis of syphilis |
| title |
Performance of Treponema pallidum recombinant proteins in the serological diagnosis of syphilis |
| spellingShingle |
Performance of Treponema pallidum recombinant proteins in the serological diagnosis of syphilis Silva, Ângelo Antônio Oliveira Treponema pallidum Syphilis Serodiagnosis Syphilis Recombinant Proteins Diagnosis Antigenic Variation Serodiagnóstico de la Sífilis Variación Antigénica Sorodiagnóstico da Sífilis Sífilis Proteínas Recombinantes Diagnóstico Variação Antigênica |
| title_short |
Performance of Treponema pallidum recombinant proteins in the serological diagnosis of syphilis |
| title_full |
Performance of Treponema pallidum recombinant proteins in the serological diagnosis of syphilis |
| title_fullStr |
Performance of Treponema pallidum recombinant proteins in the serological diagnosis of syphilis |
| title_full_unstemmed |
Performance of Treponema pallidum recombinant proteins in the serological diagnosis of syphilis |
| title_sort |
Performance of Treponema pallidum recombinant proteins in the serological diagnosis of syphilis |
| author |
Silva, Ângelo Antônio Oliveira |
| author_facet |
Silva, Ângelo Antônio Oliveira Vasconcelos, Larissa de Carvalho Medrado Foti, Leonardo Leony, Leonardo Maia Daltro, Ramona Tavares Leitolis, Amanda Lima, Fernanda Washington de Mendonça Krieger, Marco Aurélio Zanchin, Nilson Ivo Tonin Santos, Fred Luciano Neves |
| author_role |
author |
| author2 |
Vasconcelos, Larissa de Carvalho Medrado Foti, Leonardo Leony, Leonardo Maia Daltro, Ramona Tavares Leitolis, Amanda Lima, Fernanda Washington de Mendonça Krieger, Marco Aurélio Zanchin, Nilson Ivo Tonin Santos, Fred Luciano Neves |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Silva, Ângelo Antônio Oliveira Vasconcelos, Larissa de Carvalho Medrado Foti, Leonardo Leony, Leonardo Maia Daltro, Ramona Tavares Leitolis, Amanda Lima, Fernanda Washington de Mendonça Krieger, Marco Aurélio Zanchin, Nilson Ivo Tonin Santos, Fred Luciano Neves |
| dc.subject.other.pt_BR.fl_str_mv |
Treponema pallidum |
| topic |
Treponema pallidum Syphilis Serodiagnosis Syphilis Recombinant Proteins Diagnosis Antigenic Variation Serodiagnóstico de la Sífilis Variación Antigénica Sorodiagnóstico da Sífilis Sífilis Proteínas Recombinantes Diagnóstico Variação Antigênica |
| dc.subject.en.pt_BR.fl_str_mv |
Syphilis Serodiagnosis Syphilis Recombinant Proteins Diagnosis Antigenic Variation |
| dc.subject.es.pt_BR.fl_str_mv |
Serodiagnóstico de la Sífilis Variación Antigénica |
| dc.subject.decs.pt_BR.fl_str_mv |
Sorodiagnóstico da Sífilis Sífilis Proteínas Recombinantes Diagnóstico Variação Antigênica |
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Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. |
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2020 |
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2020-08-03T20:12:14Z |
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2020-08-03T20:12:14Z |
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2020 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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SILVA, Ângelo Antônio Oliveira et al. Performance of Treponema pallidum recombinant proteins in the serological diagnosis of syphilis. Plos One, v. 11, n. 6, p. 1-21, 2020. |
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https://www.arca.fiocruz.br/handle/icict/42518 |
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1932-6203 |
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10.1371/journal.pone.0234043 |
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1932-6203 |
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SILVA, Ângelo Antônio Oliveira et al. Performance of Treponema pallidum recombinant proteins in the serological diagnosis of syphilis. Plos One, v. 11, n. 6, p. 1-21, 2020. 1932-6203 10.1371/journal.pone.0234043 |
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