The dual effect of acetate on microglial TNF-α production

Detalhes bibliográficos
Autor(a) principal: Fragas, Matheus Garcia
Data de Publicação: 2022
Outros Autores: Oliveira, Daniel May de, Hiyane, Meire Ioshie, Braga, Tarcio Teodoro, Camara, Niels Olsen Saraiva
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/54832
Resumo: Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Imunologia. São Paulo, SP, Brasil.
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spelling Fragas, Matheus GarciaOliveira, Daniel May deHiyane, Meire IoshieBraga, Tarcio TeodoroCamara, Niels Olsen Saraiva2022-09-21T19:43:17Z2022-09-21T19:43:17Z2022FRAGAS, Matheus Garcia et al. The dual effect of acetate on microglial TNF-α production. Clinics, v. 77, n. 10062, p. 1–7, 2022.https://www.arca.fiocruz.br/handle/icict/5483210.1016/j.clinsp.2022.100062porElsevierTNF-aAcetatesInflammationAlzheimer DiseaseEncephalomyelitisTumor Necrosis Factor InhibitorsNeuropharmacologyMicroglíaInflamaciónEnfermedad de AlzheimerEncefalomielitisInhibidores del Factor de Necrosis TumoralNeurofarmacologíaAcétatesMicroglieInflammationMaladie d'AlzheimerEncéphalomyéliteNeuropharmacologieAcetatosMicrogliaInflamaçãoDoença de AlzheimerEncefalomieliteInibidores do Fator de Necrose TumoralNeurofarmacologiaThe dual effect of acetate on microglial TNF-α productioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Imunologia. São Paulo, SP, Brasil.Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Imunologia. São Paulo, SP, Brasil.Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Imunologia. São Paulo, SP, Brasil.Universidade Federal do Paraná. Departamento de Patologia Básica. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Programa de Pós-Graduação em Biociências e Biotecnologia. Curitiba, PR, Brasil.Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Imunologia. São Paulo, SP, Brasil. / Universidade de São Paulo. Divisão de Nefrologia. São Paulo, SP, Brasil.Short-Chain Fatty Acids (SCFA) are products of intestinal microbial metabolism that can reach the brain and alter microglia in health and disease contexts. However, data are conflicting on the effect of acetate, the most abundant SCFA in the blood, in these cells. The authors aimed to investigate acetate as a modulator of the inflammatory response in microglia stimulated with LPS. The authors used an immortalized cell line, C8-B4, and primary cells for in vitro treatments with acetate and LPS. Cell viability was analyzed by MTT, cytokine by RT-PCR, ELISA, and flow cytometry. The authors also performed in vivo and in silico analyses to study the role of acetate and the TNF-α contribution to the development of Experimental Autoimmune Encephalomyelitis (EAE). Acetate co-administered with LPS was able to exacerbate the production of pro-inflammatory cytokines at gene and protein levels in cell lines and primary culture of microglia. However, the same effects were not observed when acetate was administered alone or as pretreatment, prior to the LPS stimulus. Additionally, pharmacological inhibition of histone deacetylase concomitantly with acetate and LPS led to decreased TNF-α production. In silico analysis showed a crucial role of the TNF-α pathway in EAE development. Moreover, acetate administration in vivo during the initial phase of EAE led to a better disease outcome and reduced TNF-α production. Treatment with acetate was able to promote the production of TNF-α in a concomitant LPS stimulus of microglia. However, the immune modulation of microglia by acetate pretreatment may be a component in the generation of future therapies for neurodegenerative diseases.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/54832/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALClinics_77OK.pdfClinics_77OK.pdfapplication/pdf1177259https://www.arca.fiocruz.br/bitstream/icict/54832/2/Clinics_77OK.pdf75bbebfeacc85e84edc959a061daea61MD52icict/548322022-09-21 18:33:05.117oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-09-21T21:33:05Repositório Institucional da FIOCRUZ (ARCA) - 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dc.title.en_US.fl_str_mv The dual effect of acetate on microglial TNF-α production
title The dual effect of acetate on microglial TNF-α production
spellingShingle The dual effect of acetate on microglial TNF-α production
Fragas, Matheus Garcia
TNF-a
Acetates
Inflammation
Alzheimer Disease
Encephalomyelitis
Tumor Necrosis Factor Inhibitors
Neuropharmacology
Microglía
Inflamación
Enfermedad de Alzheimer
Encefalomielitis
Inhibidores del Factor de Necrosis Tumoral
Neurofarmacología
Acétates
Microglie
Inflammation
Maladie d'Alzheimer
Encéphalomyélite
Neuropharmacologie
Acetatos
Microglia
Inflamação
Doença de Alzheimer
Encefalomielite
Inibidores do Fator de Necrose Tumoral
Neurofarmacologia
title_short The dual effect of acetate on microglial TNF-α production
title_full The dual effect of acetate on microglial TNF-α production
title_fullStr The dual effect of acetate on microglial TNF-α production
title_full_unstemmed The dual effect of acetate on microglial TNF-α production
title_sort The dual effect of acetate on microglial TNF-α production
author Fragas, Matheus Garcia
author_facet Fragas, Matheus Garcia
Oliveira, Daniel May de
Hiyane, Meire Ioshie
Braga, Tarcio Teodoro
Camara, Niels Olsen Saraiva
author_role author
author2 Oliveira, Daniel May de
Hiyane, Meire Ioshie
Braga, Tarcio Teodoro
Camara, Niels Olsen Saraiva
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Fragas, Matheus Garcia
Oliveira, Daniel May de
Hiyane, Meire Ioshie
Braga, Tarcio Teodoro
Camara, Niels Olsen Saraiva
dc.subject.other.en_US.fl_str_mv TNF-a
topic TNF-a
Acetates
Inflammation
Alzheimer Disease
Encephalomyelitis
Tumor Necrosis Factor Inhibitors
Neuropharmacology
Microglía
Inflamación
Enfermedad de Alzheimer
Encefalomielitis
Inhibidores del Factor de Necrosis Tumoral
Neurofarmacología
Acétates
Microglie
Inflammation
Maladie d'Alzheimer
Encéphalomyélite
Neuropharmacologie
Acetatos
Microglia
Inflamação
Doença de Alzheimer
Encefalomielite
Inibidores do Fator de Necrose Tumoral
Neurofarmacologia
dc.subject.en.en_US.fl_str_mv Acetates
Inflammation
Alzheimer Disease
Encephalomyelitis
Tumor Necrosis Factor Inhibitors
Neuropharmacology
dc.subject.es.en_US.fl_str_mv Microglía
Inflamación
Enfermedad de Alzheimer
Encefalomielitis
Inhibidores del Factor de Necrosis Tumoral
Neurofarmacología
dc.subject.fr.en_US.fl_str_mv Acétates
Microglie
Inflammation
Maladie d'Alzheimer
Encéphalomyélite
Neuropharmacologie
dc.subject.decs.en_US.fl_str_mv Acetatos
Microglia
Inflamação
Doença de Alzheimer
Encefalomielite
Inibidores do Fator de Necrose Tumoral
Neurofarmacologia
description Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Imunologia. São Paulo, SP, Brasil.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-09-21T19:43:17Z
dc.date.available.fl_str_mv 2022-09-21T19:43:17Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FRAGAS, Matheus Garcia et al. The dual effect of acetate on microglial TNF-α production. Clinics, v. 77, n. 10062, p. 1–7, 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/54832
dc.identifier.doi.none.fl_str_mv 10.1016/j.clinsp.2022.100062
identifier_str_mv FRAGAS, Matheus Garcia et al. The dual effect of acetate on microglial TNF-α production. Clinics, v. 77, n. 10062, p. 1–7, 2022.
10.1016/j.clinsp.2022.100062
url https://www.arca.fiocruz.br/handle/icict/54832
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/54832/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/54832/2/Clinics_77OK.pdf
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