Genotypic Characterization of Herpes Simplex Virus Type 1 Isolates in Immunocompromised Patients in Rio de Janeiro, Brazil

Detalhes bibliográficos
Autor(a) principal: Silva, Amanda Perse da
Data de Publicação: 2015
Outros Autores: Lopes, Amanda de Oliveira, Vieira, Yasmine Rangel, Almeida, Adilson José de, Sion, Fernando Samuel, Grinsztejn, Beatriz, Wagner, Sandra, Paula, Vanessa Salete de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/13422
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
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spelling Silva, Amanda Perse daLopes, Amanda de OliveiraVieira, Yasmine RangelAlmeida, Adilson José deSion, Fernando SamuelGrinsztejn, BeatrizWagner, SandraPaula, Vanessa Salete de2016-03-31T16:06:12Z2016-03-31T16:06:12Z2015SILVA, Amanda Perse da; et al. Genotypic Characterization of Herpes Simplex Virus Type 1 Isolates in Immunocompromised Patients in Rio de Janeiro, Brazil. Plos One, v.10, n.9, e0136825, Sept. 2015.1932-6203https://www.arca.fiocruz.br/handle/icict/1342210.1371/journal.pone.0136825engPublic Library of ScienceGenotypic Characterization of Herpes Simplex Virus Type 1 Isolates in Immunocompromised Patients in Rio de Janeiro, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Universidade Federal do Estado do Rio de Janeiro (UNIRIO). Hospital Universitário Gafreé Guinle. Rio de Janeiro, RJ, Brasil.Universidade Federal do Estado do Rio de Janeiro (UNIRIO). Hospital Universitário Gafreé Guinle. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas (INI). Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas (INI). Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Herpes simplex virus type 1 (HSV-1) is a prevalent human pathogen that causes a variety of diseases, including an increased risk of developing more severe disease in HIV-infected individuals. In Brazil, there is no information about the molecular epidemiology of HSV-1 infection, especially in HIV-infected individuals. The aim of this study was to perform the genotypic characterization of HSV-1 among HIV-infected patients. A total of 214 serum samples from HIV-positive patients without HSV infection symptoms were enrolled in one of two reference hospitals for HIV infection managing in Rio de Janeiro. The gG and gI genes were analyzed by restriction fragment length polymorphism (RFLP) and full nucleotide sequencing of the US8 (1601 bp), UL44 (1996 bp), and UL23 (1244 bp) regions was performed. A total of 38.3% (82/214) and 32.7% (70/214) of the serum samples tested positive for gG and gI genes, respectively. RFLP analysis classified the HSV-1 as belonging to genotype A. Phylogenetic analysis of the Brazilian samples for the US8, UL44, and UL23 regions demonstrated that the nucleotide identity between Brazilian samples was higher than 97% for all genes. No acyclovir mutation was detected in the patients. The shedding of HSV in the serum samples from HIV-positive patients who were asymptomatic for HSV infection was detected in this work. This is the first report of molecular characterization of HSV-1 in Brazilian samples since there is no previous data available in the literature concerning the genotypic classification and stable distribution of Brazilian strains of HSV-1 in Rio de Janeiro, Brazil.Herpes Simplex Virus Type 1Rio de JaneiroGenotypic CharacterizationHIV-infected patientsHerpesvirus Humano 1Rio de Janeiroinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/13422/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALamanda_silva_etal_IOC_2015.pdfamanda_silva_etal_IOC_2015.pdfapplication/pdf681417https://www.arca.fiocruz.br/bitstream/icict/13422/2/amanda_silva_etal_IOC_2015.pdff806036b56dc0c91941b7322189819dbMD52TEXTamanda_silva_etal_IOC_2015.pdf.txtamanda_silva_etal_IOC_2015.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Genotypic Characterization of Herpes Simplex Virus Type 1 Isolates in Immunocompromised Patients in Rio de Janeiro, Brazil
title Genotypic Characterization of Herpes Simplex Virus Type 1 Isolates in Immunocompromised Patients in Rio de Janeiro, Brazil
spellingShingle Genotypic Characterization of Herpes Simplex Virus Type 1 Isolates in Immunocompromised Patients in Rio de Janeiro, Brazil
Silva, Amanda Perse da
Herpes Simplex Virus Type 1
Rio de Janeiro
Genotypic Characterization
HIV-infected patients
Herpesvirus Humano 1
Rio de Janeiro
title_short Genotypic Characterization of Herpes Simplex Virus Type 1 Isolates in Immunocompromised Patients in Rio de Janeiro, Brazil
title_full Genotypic Characterization of Herpes Simplex Virus Type 1 Isolates in Immunocompromised Patients in Rio de Janeiro, Brazil
title_fullStr Genotypic Characterization of Herpes Simplex Virus Type 1 Isolates in Immunocompromised Patients in Rio de Janeiro, Brazil
title_full_unstemmed Genotypic Characterization of Herpes Simplex Virus Type 1 Isolates in Immunocompromised Patients in Rio de Janeiro, Brazil
title_sort Genotypic Characterization of Herpes Simplex Virus Type 1 Isolates in Immunocompromised Patients in Rio de Janeiro, Brazil
author Silva, Amanda Perse da
author_facet Silva, Amanda Perse da
Lopes, Amanda de Oliveira
Vieira, Yasmine Rangel
Almeida, Adilson José de
Sion, Fernando Samuel
Grinsztejn, Beatriz
Wagner, Sandra
Paula, Vanessa Salete de
author_role author
author2 Lopes, Amanda de Oliveira
Vieira, Yasmine Rangel
Almeida, Adilson José de
Sion, Fernando Samuel
Grinsztejn, Beatriz
Wagner, Sandra
Paula, Vanessa Salete de
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Amanda Perse da
Lopes, Amanda de Oliveira
Vieira, Yasmine Rangel
Almeida, Adilson José de
Sion, Fernando Samuel
Grinsztejn, Beatriz
Wagner, Sandra
Paula, Vanessa Salete de
dc.subject.en.pt_BR.fl_str_mv Herpes Simplex Virus Type 1
Rio de Janeiro
Genotypic Characterization
HIV-infected patients
topic Herpes Simplex Virus Type 1
Rio de Janeiro
Genotypic Characterization
HIV-infected patients
Herpesvirus Humano 1
Rio de Janeiro
dc.subject.decs.pt_BR.fl_str_mv Herpesvirus Humano 1
Rio de Janeiro
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-03-31T16:06:12Z
dc.date.available.fl_str_mv 2016-03-31T16:06:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA, Amanda Perse da; et al. Genotypic Characterization of Herpes Simplex Virus Type 1 Isolates in Immunocompromised Patients in Rio de Janeiro, Brazil. Plos One, v.10, n.9, e0136825, Sept. 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/13422
dc.identifier.issn.none.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0136825
identifier_str_mv SILVA, Amanda Perse da; et al. Genotypic Characterization of Herpes Simplex Virus Type 1 Isolates in Immunocompromised Patients in Rio de Janeiro, Brazil. Plos One, v.10, n.9, e0136825, Sept. 2015.
1932-6203
10.1371/journal.pone.0136825
url https://www.arca.fiocruz.br/handle/icict/13422
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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