Natural circulation of human enterovirus in Maputo city, Mozambique
Autor(a) principal: | |
---|---|
Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/13951 |
Resumo: | Ministério da Saúde. Instituto Nacional de Saúde de Moçambique. Maputo, Moçambique. |
id |
CRUZ_7eff4311a1170e8af8389facc2a0cd18 |
---|---|
oai_identifier_str |
oai:www.arca.fiocruz.br:icict/13951 |
network_acronym_str |
CRUZ |
network_name_str |
Repositório Institucional da FIOCRUZ (ARCA) |
repository_id_str |
2135 |
spelling |
Bero, Diocreciano M.Deus, Nilsa deCosta, Eliane V. daBurlandy, Fernanda M.Jani, Ilesh V.Silva, Edson E. da2016-04-24T16:53:33Z2016-04-24T16:53:33Z2015BERO, Diocreciano M. et al. Natural circulation of human enterovirus in Maputo city, Mozambique. African Journal of Microbiology Research, v.9, n.21, p.1419-1423, May 2015.1996-0808https://www.arca.fiocruz.br/handle/icict/1395110.5897/AJMR2015.7531engAcademicJournalsNatural circulation of human enterovirus in Maputo city, Mozambiqueinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMinistério da Saúde. Instituto Nacional de Saúde de Moçambique. Maputo, Moçambique.Ministério da Saúde. Instituto Nacional de Saúde de Moçambique. Maputo, Moçambique.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Entovírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Entovírus. Rio de Janeiro, RJ, Brasil.Ministério da Saúde. Instituto Nacional de Saúde de Moçambique. Maputo, Moçambique.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Entovírus. Rio de Janeiro, RJ, Brasil.The human enteroviruses (HEV) are responsible for a large diversity of infections affecting humans. Most infections are asymptomatic, but these viruses can cause a wide spectrum of diseases, including severe cases involving the central nervous system. The aim of this study was to isolate and identify human enteroviruses in natural circulation in children less than 15 years of age admitted at the Mavalane General Hospital in Maputo City, Mozambique. In this study, 178 stool samples were processed, obtained during November 2011 to February 2012. Samples were inoculated onto cell culture and the isolates were identified as enterovirus by conventional RT-PCR in the 5’ non-coding region followed by partial VP1 sequence. Twenty-six (26) out of the 45 cell-culture positive samples were constituted by Enterovirus (14.6% of the total 178 samples). EV-29 was the serotype most prevalent. The results show the importance of maintaining the cell line Hep2C in the diagnosis and Enterovirus circulating in the Maputo city, Mozambique.EnterovirusStoolCell culturecell line Hep2CHospital Geral de MavalaneEnterovírusInfecções por EnterovirusTécnicas de Cultura de CélulasLinhagem Celularinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/13951/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALfernanda_burlandy_etal_IOC_2015.pdffernanda_burlandy_etal_IOC_2015.pdfapplication/pdf425818https://www.arca.fiocruz.br/bitstream/icict/13951/2/fernanda_burlandy_etal_IOC_2015.pdf55c629a7223867195c46cb87c6a562ddMD52TEXTfernanda_burlandy_etal_IOC_2015.pdf.txtfernanda_burlandy_etal_IOC_2015.pdf.txtExtracted texttext/plain22086https://www.arca.fiocruz.br/bitstream/icict/13951/3/fernanda_burlandy_etal_IOC_2015.pdf.txt18df7d157d888fdc4e3faa8569e32e0bMD53icict/139512023-09-14 15:53:59.191oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-14T18:53:59Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.pt_BR.fl_str_mv |
Natural circulation of human enterovirus in Maputo city, Mozambique |
title |
Natural circulation of human enterovirus in Maputo city, Mozambique |
spellingShingle |
Natural circulation of human enterovirus in Maputo city, Mozambique Bero, Diocreciano M. Enterovirus Stool Cell culture cell line Hep2C Hospital Geral de Mavalane Enterovírus Infecções por Enterovirus Técnicas de Cultura de Células Linhagem Celular |
title_short |
Natural circulation of human enterovirus in Maputo city, Mozambique |
title_full |
Natural circulation of human enterovirus in Maputo city, Mozambique |
title_fullStr |
Natural circulation of human enterovirus in Maputo city, Mozambique |
title_full_unstemmed |
Natural circulation of human enterovirus in Maputo city, Mozambique |
title_sort |
Natural circulation of human enterovirus in Maputo city, Mozambique |
author |
Bero, Diocreciano M. |
author_facet |
Bero, Diocreciano M. Deus, Nilsa de Costa, Eliane V. da Burlandy, Fernanda M. Jani, Ilesh V. Silva, Edson E. da |
author_role |
author |
author2 |
Deus, Nilsa de Costa, Eliane V. da Burlandy, Fernanda M. Jani, Ilesh V. Silva, Edson E. da |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Bero, Diocreciano M. Deus, Nilsa de Costa, Eliane V. da Burlandy, Fernanda M. Jani, Ilesh V. Silva, Edson E. da |
dc.subject.en.pt_BR.fl_str_mv |
Enterovirus Stool Cell culture cell line Hep2C Hospital Geral de Mavalane |
topic |
Enterovirus Stool Cell culture cell line Hep2C Hospital Geral de Mavalane Enterovírus Infecções por Enterovirus Técnicas de Cultura de Células Linhagem Celular |
dc.subject.decs.pt_BR.fl_str_mv |
Enterovírus Infecções por Enterovirus Técnicas de Cultura de Células Linhagem Celular |
description |
Ministério da Saúde. Instituto Nacional de Saúde de Moçambique. Maputo, Moçambique. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015 |
dc.date.accessioned.fl_str_mv |
2016-04-24T16:53:33Z |
dc.date.available.fl_str_mv |
2016-04-24T16:53:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
BERO, Diocreciano M. et al. Natural circulation of human enterovirus in Maputo city, Mozambique. African Journal of Microbiology Research, v.9, n.21, p.1419-1423, May 2015. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/13951 |
dc.identifier.issn.none.fl_str_mv |
1996-0808 |
dc.identifier.doi.none.fl_str_mv |
10.5897/AJMR2015.7531 |
identifier_str_mv |
BERO, Diocreciano M. et al. Natural circulation of human enterovirus in Maputo city, Mozambique. African Journal of Microbiology Research, v.9, n.21, p.1419-1423, May 2015. 1996-0808 10.5897/AJMR2015.7531 |
url |
https://www.arca.fiocruz.br/handle/icict/13951 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
AcademicJournals |
publisher.none.fl_str_mv |
AcademicJournals |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
reponame_str |
Repositório Institucional da FIOCRUZ (ARCA) |
collection |
Repositório Institucional da FIOCRUZ (ARCA) |
bitstream.url.fl_str_mv |
https://www.arca.fiocruz.br/bitstream/icict/13951/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/13951/2/fernanda_burlandy_etal_IOC_2015.pdf https://www.arca.fiocruz.br/bitstream/icict/13951/3/fernanda_burlandy_etal_IOC_2015.pdf.txt |
bitstream.checksum.fl_str_mv |
5a560609d32a3863062d77ff32785d58 55c629a7223867195c46cb87c6a562dd 18df7d157d888fdc4e3faa8569e32e0b |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ) |
repository.mail.fl_str_mv |
repositorio.arca@fiocruz.br |
_version_ |
1813009107110592512 |