Influence of Serratia marcescens and Rhodococcus rhodnii on the humoral immunity of Rhodnius prolixus
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/49563 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil. |
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Batista, Kate K. S.Vieira, Cecília S.Figueiredo, Marcela B.Costa-Latgé, Samara G.Azambuja, PatríciaGenta, Fernando A.Castro, Daniele P.2021-10-25T22:28:46Z2021-10-25T22:28:46Z2021BATISTA, Kate K. S. et al. Influence of Serratia marcescens and Rhodococcus rhodnii on the humoral immunity of Rhodnius prolixus. International Journal of Molecular Sciences, v. 22, n. 20, p. 1-17, 9 Oct. 2021.1661-6596https://www.arca.fiocruz.br/handle/icict/4956310.3390/ijms2220109011422-0067engMDPIImunidade a insetosTriatomíneoMicrobiotaBacteriaFisiologia do insetoAntibióticoAposimbióticoInsect immunityTriatomineMicrobiotaBacteriaInsect physiologyAntibioticAposimbioticInfluence of Serratia marcescens and Rhodococcus rhodnii on the humoral immunity of Rhodnius prolixusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil.Institute of Life Science. Swansea University Medical School. Swansea. UK.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense. Instituto de Biologia. Programa de Pós-Graduação em Ciências e Biotecnologia. Niterói, RJ, Brasil / Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Bioquímica Médica Leopoldo de Meis. Instituto Nacional de Entomologia Molecular. Departamento de Entomologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Bioquímica Médica Leopoldo de Meis. Instituto Nacional de Entomologia Molecular. Departamento de Entomologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Bioquímica Médica Leopoldo de Meis. Instituto Nacional de Entomologia Molecular. Departamento de Entomologia Molecular. Rio de Janeiro, RJ, Brasil.Chagas disease is a human infectious disease caused by Trypanosoma cruzi and can be transmitted by triatomine vectors, such as Rhodnius prolixus. One limiting factor for T. cruzi development is the composition of the bacterial gut microbiota in the triatomine. Herein, we analyzed the humoral immune responses of R. prolixus nymphs treated with antibiotics and subsequently recolonized with either Serratia marcescens or Rhodococcus rhodnii. The treatment with antibiotics reduced the bacterial load in the digestive tract, and the recolonization with each bacterium was successfully detected seven days after treatment. The antibiotic-treated insects, recolonized with S. marcescens, presented reduced antibacterial activity against Staphylococcus aureus and phenoloxidase activity in hemolymph, and lower nitric oxide synthase (NOS) and higher defensin C gene (DefC) gene expression in the fat body. These insects also presented a higher expression of DefC, lower prolixicin (Prol), and lower NOS levels in the anterior midgut. However, the antibiotic-treated insects recolonized with R. rhodnii had increased antibacterial activity against Escherichia coli and lower activity against S. aureus, higher phenoloxidase activity in hemolymph, and lower NOS expression in the fat body. In the anterior midgut, these insects presented higher NOS, defensin A (DefA) and DefC expression, and lower Prol expression. The R. prolixus immune modulation by these two bacteria was observed not only in the midgut, but also systemically in the fat body, and may be crucial for the development and transmission of the parasites Trypanosoma cruzi and Trypanosoma rangeli.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/49563/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALKateBatista_CeciliaVieira_IOC_etal_2021.pdfKateBatista_CeciliaVieira_IOC_etal_2021.pdfapplication/pdf2215572https://www.arca.fiocruz.br/bitstream/icict/49563/2/KateBatista_CeciliaVieira_IOC_etal_2021.pdf81f2ace16b04def863671599e539ae53MD52icict/495632022-06-24 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| dc.title.en.fl_str_mv |
Influence of Serratia marcescens and Rhodococcus rhodnii on the humoral immunity of Rhodnius prolixus |
| title |
Influence of Serratia marcescens and Rhodococcus rhodnii on the humoral immunity of Rhodnius prolixus |
| spellingShingle |
Influence of Serratia marcescens and Rhodococcus rhodnii on the humoral immunity of Rhodnius prolixus Batista, Kate K. S. Imunidade a insetos Triatomíneo Microbiota Bacteria Fisiologia do inseto Antibiótico Aposimbiótico Insect immunity Triatomine Microbiota Bacteria Insect physiology Antibiotic Aposimbiotic |
| title_short |
Influence of Serratia marcescens and Rhodococcus rhodnii on the humoral immunity of Rhodnius prolixus |
| title_full |
Influence of Serratia marcescens and Rhodococcus rhodnii on the humoral immunity of Rhodnius prolixus |
| title_fullStr |
Influence of Serratia marcescens and Rhodococcus rhodnii on the humoral immunity of Rhodnius prolixus |
| title_full_unstemmed |
Influence of Serratia marcescens and Rhodococcus rhodnii on the humoral immunity of Rhodnius prolixus |
| title_sort |
Influence of Serratia marcescens and Rhodococcus rhodnii on the humoral immunity of Rhodnius prolixus |
| author |
Batista, Kate K. S. |
| author_facet |
Batista, Kate K. S. Vieira, Cecília S. Figueiredo, Marcela B. Costa-Latgé, Samara G. Azambuja, Patrícia Genta, Fernando A. Castro, Daniele P. |
| author_role |
author |
| author2 |
Vieira, Cecília S. Figueiredo, Marcela B. Costa-Latgé, Samara G. Azambuja, Patrícia Genta, Fernando A. Castro, Daniele P. |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Batista, Kate K. S. Vieira, Cecília S. Figueiredo, Marcela B. Costa-Latgé, Samara G. Azambuja, Patrícia Genta, Fernando A. Castro, Daniele P. |
| dc.subject.other.pt_BR.fl_str_mv |
Imunidade a insetos Triatomíneo Microbiota Bacteria Fisiologia do inseto Antibiótico Aposimbiótico |
| topic |
Imunidade a insetos Triatomíneo Microbiota Bacteria Fisiologia do inseto Antibiótico Aposimbiótico Insect immunity Triatomine Microbiota Bacteria Insect physiology Antibiotic Aposimbiotic |
| dc.subject.en.en.fl_str_mv |
Insect immunity Triatomine Microbiota Bacteria Insect physiology Antibiotic Aposimbiotic |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil. |
| publishDate |
2021 |
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2021-10-25T22:28:46Z |
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2021-10-25T22:28:46Z |
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2021 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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BATISTA, Kate K. S. et al. Influence of Serratia marcescens and Rhodococcus rhodnii on the humoral immunity of Rhodnius prolixus. International Journal of Molecular Sciences, v. 22, n. 20, p. 1-17, 9 Oct. 2021. |
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https://www.arca.fiocruz.br/handle/icict/49563 |
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1661-6596 |
| dc.identifier.doi.pt_BR.fl_str_mv |
10.3390/ijms222010901 |
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1422-0067 |
| identifier_str_mv |
BATISTA, Kate K. S. et al. Influence of Serratia marcescens and Rhodococcus rhodnii on the humoral immunity of Rhodnius prolixus. International Journal of Molecular Sciences, v. 22, n. 20, p. 1-17, 9 Oct. 2021. 1661-6596 10.3390/ijms222010901 1422-0067 |
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MDPI |
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MDPI |
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