In VitroImmunomodulatory Activity of a Transition-State Analog Inhibitor of Human Purine Nucleoside Phosphorylase in Cutaneous Leishmaniasis

Detalhes bibliográficos
Autor(a) principal: Carvalho, Natália Barbosa
Data de Publicação: 2017
Outros Autores: Prates, Fernanda Ventin de Oliveira, Silva, Rafael de Castro da, Dourado, Mayra Elizabeth Ferreira, Amorim, Camila Farias, Machado, Paulo Roberto Lima, Pacheco, Fernanda Grendene, Corte, Temis Weber Furlanetto, Machado, Pablo, Santos, Diógenes Santiago, Carvalho, Edgar Marcelino de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/24905
Resumo: Ministry of Health, National Council for Scientific and Technological Development (CNPq) from Brazil (467207/2014-3), and Coordination for the Improvement of Higher Education Personnel (CAPES).
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spelling Carvalho, Natália BarbosaPrates, Fernanda Ventin de OliveiraSilva, Rafael de Castro daDourado, Mayra Elizabeth FerreiraAmorim, Camila FariasMachado, Paulo Roberto LimaPacheco, Fernanda GrendeneCorte, Temis Weber FurlanettoMachado, PabloSantos, Diógenes SantiagoCarvalho, Edgar Marcelino de2018-02-21T19:27:05Z2018-02-21T19:27:05Z2017CARVALHO, N. B. et al. In VitroImmunomodulatory Activity of a Transition-State Analog Inhibitor of Human Purine Nucleoside Phosphorylase in Cutaneous Leishmaniasis. Journal of Immunology Research, 3062892, 2017.2314-8861https://www.arca.fiocruz.br/handle/icict/2490510.1155/2017/3062892Ministry of Health, National Council for Scientific and Technological Development (CNPq) from Brazil (467207/2014-3), and Coordination for the Improvement of Higher Education Personnel (CAPES).Universidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilPontifícia Universidade Católica do Rio Grande do Sul. Centro de Pesquisas em Biologia Molecular e Funcional. Porto Alegre, RS, BrasilPontifícia Universidade Católica do Rio Grande do Sul. Centro de Pesquisas em Biologia Molecular e Funcional. Porto Alegre, RS, BrasilPontifícia Universidade Católica do Rio Grande do Sul. Centro de Pesquisas em Biologia Molecular e Funcional. Porto Alegre, RS, BrasilPontifícia Universidade Católica do Rio Grande do Sul. Centro de Pesquisas em Biologia Molecular e Funcional. Porto Alegre, RS, BrasilUniversidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilCutaneous leishmaniasis (CL) is the most common clinical form of American tegumentary leishmaniasis caused byLeishmania(Viannia)braziliensis. CL is associated with a strong Th1 immune response. This exacerbated inflammatory response is correlated with severity of disease and delays the healing time of the ulcer. The fourth-generation immucillin derivative (DI4G), a potent inhibitor of purine nucleoside phosphorylase, has been proposed as a promising agent in the treatment of diseases associated with T cell activation. Herein, we evaluated thein vitroimmunomodulatory activity of DI4G in cells of patients presenting with CL. Peripheral blood mononuclear cells (PBMC) from CL patients were stimulated with soluble leishmania antigen (SLA), in the presence or absence of DI4G, and IFN-γ, TNF, CXCL9, and CXCL10 levels were determined by ELISA. Lymphocyte proliferation in the presence or absence of DI4G was also evaluated, using flow cytometry. DI4G was able to decrease (p< 0.05) IFN-γproduction but did not change the TNF, CXCL9, and CXCL10 levels. DI4G decreased (p< 0.05) the lymphoproliferative response mediated by CD8+T cells, but not that by CD4+T cells. DI4G is able to attenuate the exaggerated immune response in CL, exhibiting immunomodulatory activity in IFN-γproduction and in CD8+T cell proliferation.engHindawi Publishing CorporationLeishmaniose CutâneaPurina-nucleósideo fosforilaseLinfócitosLeishmaniaCitometria de fluxoHumanosCutaneous LeishmaniasisPurine Nucleoside PhosphorylaseLymphocyteLeishmaniaFlow cytometryHumansIn VitroImmunomodulatory Activity of a Transition-State Analog Inhibitor of Human Purine Nucleoside Phosphorylase in Cutaneous Leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/24905/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALCarvalho NB In Vitro Immunomodulatory Activity....pdfCarvalho NB In Vitro Immunomodulatory 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dc.title.pt_BR.fl_str_mv In VitroImmunomodulatory Activity of a Transition-State Analog Inhibitor of Human Purine Nucleoside Phosphorylase in Cutaneous Leishmaniasis
title In VitroImmunomodulatory Activity of a Transition-State Analog Inhibitor of Human Purine Nucleoside Phosphorylase in Cutaneous Leishmaniasis
spellingShingle In VitroImmunomodulatory Activity of a Transition-State Analog Inhibitor of Human Purine Nucleoside Phosphorylase in Cutaneous Leishmaniasis
Carvalho, Natália Barbosa
Leishmaniose Cutânea
Purina-nucleósideo fosforilase
Linfócitos
Leishmania
Citometria de fluxo
Humanos
Cutaneous Leishmaniasis
Purine Nucleoside Phosphorylase
Lymphocyte
Leishmania
Flow cytometry
Humans
title_short In VitroImmunomodulatory Activity of a Transition-State Analog Inhibitor of Human Purine Nucleoside Phosphorylase in Cutaneous Leishmaniasis
title_full In VitroImmunomodulatory Activity of a Transition-State Analog Inhibitor of Human Purine Nucleoside Phosphorylase in Cutaneous Leishmaniasis
title_fullStr In VitroImmunomodulatory Activity of a Transition-State Analog Inhibitor of Human Purine Nucleoside Phosphorylase in Cutaneous Leishmaniasis
title_full_unstemmed In VitroImmunomodulatory Activity of a Transition-State Analog Inhibitor of Human Purine Nucleoside Phosphorylase in Cutaneous Leishmaniasis
title_sort In VitroImmunomodulatory Activity of a Transition-State Analog Inhibitor of Human Purine Nucleoside Phosphorylase in Cutaneous Leishmaniasis
author Carvalho, Natália Barbosa
author_facet Carvalho, Natália Barbosa
Prates, Fernanda Ventin de Oliveira
Silva, Rafael de Castro da
Dourado, Mayra Elizabeth Ferreira
Amorim, Camila Farias
Machado, Paulo Roberto Lima
Pacheco, Fernanda Grendene
Corte, Temis Weber Furlanetto
Machado, Pablo
Santos, Diógenes Santiago
Carvalho, Edgar Marcelino de
author_role author
author2 Prates, Fernanda Ventin de Oliveira
Silva, Rafael de Castro da
Dourado, Mayra Elizabeth Ferreira
Amorim, Camila Farias
Machado, Paulo Roberto Lima
Pacheco, Fernanda Grendene
Corte, Temis Weber Furlanetto
Machado, Pablo
Santos, Diógenes Santiago
Carvalho, Edgar Marcelino de
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Carvalho, Natália Barbosa
Prates, Fernanda Ventin de Oliveira
Silva, Rafael de Castro da
Dourado, Mayra Elizabeth Ferreira
Amorim, Camila Farias
Machado, Paulo Roberto Lima
Pacheco, Fernanda Grendene
Corte, Temis Weber Furlanetto
Machado, Pablo
Santos, Diógenes Santiago
Carvalho, Edgar Marcelino de
dc.subject.other.pt_BR.fl_str_mv Leishmaniose Cutânea
Purina-nucleósideo fosforilase
Linfócitos
Leishmania
Citometria de fluxo
Humanos
topic Leishmaniose Cutânea
Purina-nucleósideo fosforilase
Linfócitos
Leishmania
Citometria de fluxo
Humanos
Cutaneous Leishmaniasis
Purine Nucleoside Phosphorylase
Lymphocyte
Leishmania
Flow cytometry
Humans
dc.subject.en.pt_BR.fl_str_mv Cutaneous Leishmaniasis
Purine Nucleoside Phosphorylase
Lymphocyte
Leishmania
Flow cytometry
Humans
description Ministry of Health, National Council for Scientific and Technological Development (CNPq) from Brazil (467207/2014-3), and Coordination for the Improvement of Higher Education Personnel (CAPES).
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2018-02-21T19:27:05Z
dc.date.available.fl_str_mv 2018-02-21T19:27:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CARVALHO, N. B. et al. In VitroImmunomodulatory Activity of a Transition-State Analog Inhibitor of Human Purine Nucleoside Phosphorylase in Cutaneous Leishmaniasis. Journal of Immunology Research, 3062892, 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/24905
dc.identifier.issn.pt_BR.fl_str_mv 2314-8861
dc.identifier.doi.none.fl_str_mv 10.1155/2017/3062892
identifier_str_mv CARVALHO, N. B. et al. In VitroImmunomodulatory Activity of a Transition-State Analog Inhibitor of Human Purine Nucleoside Phosphorylase in Cutaneous Leishmaniasis. Journal of Immunology Research, 3062892, 2017.
2314-8861
10.1155/2017/3062892
url https://www.arca.fiocruz.br/handle/icict/24905
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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