Detection of Hepatitis B Virus Antigens in Paraffin-embedded Liver Specimens from the Amazon Region, Brazil

Detalhes bibliográficos
Autor(a) principal: Simonetti, S. R. R.
Data de Publicação: 2002
Outros Autores: Schatzmayr, H. G., Barth, Ortrud Monika, Simonetti, J. P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/44174
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.
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spelling Simonetti, S. R. R.Schatzmayr, H. G.Barth, Ortrud MonikaSimonetti, J. P.2020-10-25T18:14:38Z2020-10-25T18:14:38Z2002SIMONETTI, S. R. R. et al. Detection of Hepatitis B Virus Antigens in Paraffin-embedded Liver Specimens from the Amazon Region, Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 97, n. 1, p. 105-107, Jan. 2002.0074-0276https://www.arca.fiocruz.br/handle/icict/4417410.1590/S0074-027620020001000181678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Vírus da Hepatite BFígado humanoRegião AmazônicaBrasilHepatitis B virushuman liveAmazonBrazilDetection of Hepatitis B Virus Antigens in Paraffin-embedded Liver Specimens from the Amazon Region, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Hepatic viscerotomy of paraffin-preserved old specimens, collected in the period from 1934 to 1967, were analyzed by immunohistochemical assays to detect hepatitis B, hepatitis D, dengue and yellow fever virus antigens. The material belongs to the Yellow Fever Collection, Department of Pathology, Instituto Oswaldo Cruz, Rio de Janeiro, Brazil and the cases were diagnosed at that time according to clinical aspects and histopathological findings reporting viral hepatitis, yellow fever, focal necrosis and hepatic atrophy. From the 79 specimens, 69 were collected at the Labrea Region and the other 10 in different other localities in the Amazon Region. The five micra thick histological slices were analyzed for the presence of hepatitis B surface antigen (HBsAg) and hepatitis B core antigen (HBcAg) by immunoperoxidase technique. An immunofluorescence assay was applied to the detection of hepatitis D, yellow fever and dengue virus antigens. Nine (11.4%) histological samples were HBsAg reactive and 5 (6.3%) were HBcAg reactive. The oldest reactive sample was from 1934. Viral antigens related to the other pathologies were not detected in this study. Our results confirm that the methodology described may be used to elucidate the aetiology of hepatitis diseases even after a long time of conservation of the specimens.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/44174/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSRRSImonetti_OMBarth_etal_IOC_2002.pdfSRRSImonetti_OMBarth_etal_IOC_2002.pdfapplication/pdf85355https://www.arca.fiocruz.br/bitstream/icict/44174/2/SRRSImonetti_OMBarth_etal_IOC_2002.pdf292f055ea4c0c64a99c195b40b931edfMD52TEXTSRRSImonetti_OMBarth_etal_IOC_2002.pdf.txtSRRSImonetti_OMBarth_etal_IOC_2002.pdf.txtExtracted texttext/plain15613https://www.arca.fiocruz.br/bitstream/icict/44174/3/SRRSImonetti_OMBarth_etal_IOC_2002.pdf.txtd9395a3faf60e08a1759e1299834bd8aMD53icict/441742020-10-26 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dc.title.pt_BR.fl_str_mv Detection of Hepatitis B Virus Antigens in Paraffin-embedded Liver Specimens from the Amazon Region, Brazil
title Detection of Hepatitis B Virus Antigens in Paraffin-embedded Liver Specimens from the Amazon Region, Brazil
spellingShingle Detection of Hepatitis B Virus Antigens in Paraffin-embedded Liver Specimens from the Amazon Region, Brazil
Simonetti, S. R. R.
Vírus da Hepatite B
Fígado humano
Região Amazônica
Brasil
Hepatitis B virus
human live
Amazon
Brazil
title_short Detection of Hepatitis B Virus Antigens in Paraffin-embedded Liver Specimens from the Amazon Region, Brazil
title_full Detection of Hepatitis B Virus Antigens in Paraffin-embedded Liver Specimens from the Amazon Region, Brazil
title_fullStr Detection of Hepatitis B Virus Antigens in Paraffin-embedded Liver Specimens from the Amazon Region, Brazil
title_full_unstemmed Detection of Hepatitis B Virus Antigens in Paraffin-embedded Liver Specimens from the Amazon Region, Brazil
title_sort Detection of Hepatitis B Virus Antigens in Paraffin-embedded Liver Specimens from the Amazon Region, Brazil
author Simonetti, S. R. R.
author_facet Simonetti, S. R. R.
Schatzmayr, H. G.
Barth, Ortrud Monika
Simonetti, J. P.
author_role author
author2 Schatzmayr, H. G.
Barth, Ortrud Monika
Simonetti, J. P.
author2_role author
author
author
dc.contributor.author.fl_str_mv Simonetti, S. R. R.
Schatzmayr, H. G.
Barth, Ortrud Monika
Simonetti, J. P.
dc.subject.other.pt_BR.fl_str_mv Vírus da Hepatite B
Fígado humano
Região Amazônica
Brasil
topic Vírus da Hepatite B
Fígado humano
Região Amazônica
Brasil
Hepatitis B virus
human live
Amazon
Brazil
dc.subject.en.pt_BR.fl_str_mv Hepatitis B virus
human live
Amazon
Brazil
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.
publishDate 2002
dc.date.issued.fl_str_mv 2002
dc.date.accessioned.fl_str_mv 2020-10-25T18:14:38Z
dc.date.available.fl_str_mv 2020-10-25T18:14:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SIMONETTI, S. R. R. et al. Detection of Hepatitis B Virus Antigens in Paraffin-embedded Liver Specimens from the Amazon Region, Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 97, n. 1, p. 105-107, Jan. 2002.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/44174
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762002000100018
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv SIMONETTI, S. R. R. et al. Detection of Hepatitis B Virus Antigens in Paraffin-embedded Liver Specimens from the Amazon Region, Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 97, n. 1, p. 105-107, Jan. 2002.
0074-0276
10.1590/S0074-02762002000100018
1678-8060
url https://www.arca.fiocruz.br/handle/icict/44174
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
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