Detection of parasite antigens in Leishmania infantum-infected spleen tissue by monoclonal antibody-, piezoelectric-based immunosensors.

Detalhes bibliográficos
Autor(a) principal: Miranda, Gustavo Cabral
Data de Publicação: 2014
Outros Autores: Jesus, Joilson Ramos de, Oliveira, Pablo Rafael Silveira, Britto, G. S. G, Pontes-de-Carvalho, Lain Carlos, Dutra, R. F, Neves, Neuza Maria Alcântara
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10434
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil
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spelling Miranda, Gustavo CabralJesus, Joilson Ramos deOliveira, Pablo Rafael SilveiraBritto, G. S. GPontes-de-Carvalho, Lain CarlosDutra, R. FNeves, Neuza Maria Alcântara2015-05-19T17:06:38Z2015-05-19T17:06:38Z2014MIRANDA, G. C. et al. Detection of parasite antigens in Leishmania infantum-infected spleen tissue by monoclonal antibody-, piezoelectric-based immunosensors. Journal of Parasitology, v. 100, n. 1, p. 73-78, 2014.1937-2345https://www.arca.fiocruz.br/handle/icict/10434dx.doi.org/10.1645/GE-3052.1engAmerican Society of ParasitologistsDetection of parasite antigens in Leishmania infantum-infected spleen tissue by monoclonal antibody-, piezoelectric-based immunosensors.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilUniversidade Federal de Pernambuco. Laboratório de EngenhariaBiomédica. Recife, PE, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Ciências da Biointeração. Salvador, BA, BrasilDiseases such as leishmaniases are important causes of morbidity and mortality in Brazil, and their diagnoses need to be improved. The use of monoclonal antibodies has ensured high specificity to immunodiagnosis. The development of an immunosensor, coupling a monoclonal antibody to a bioelectronic device capable of quickly detecting Leishmania sp. antigens both qualitatively and quantitatively, is a promising alternative for the diagnosis of leishmaniasis due to its high specificity, low cost, and portability, compared with conventional methods. The present work was aimed at developing an immunosensor-based assay for detecting Leishmania infantum antigens in tissues of infected hosts. Four hybridomas producing monoclonal antibodies against L. infantum had their specificity confirmed by enzyme-linked immunosorbent assay. These antibodies were immobilized on a gold surface, covered with a thin film of 2-aminoethanethiol (cysteamine) and glutaraldehyde, blocked with glycine, and placed into contact with extracts of L. infantum -infected and noninfected control hamster spleens. The assay was able to detect 1.8 × 10(4) amastigotes/g of infected tissue. These results demonstrated that this assay may be useful for quantifying L. infantum amastigotes in organs of experimental animals for studies on pathogenesis and immunity and that it is a promising tool for the development of a diagnostic method, based on antigen detection, of human and dog visceral leishmaniasis.Anticorpos Monoclonais/uso diagnósticoAntígenos de Protozoários/isolamento & purificaçãoLeishmania infantum/imunologiaLeishmaniose Visceral/diagnósticoBaço/parasitologiaAnimaisAnticorpos Monoclonais/imunologiaAnticorpos Antiprotozoários/uso diagnósticoEspecificidade de AnticorposTécnicas Biossensoriais/métodosCricetinaeEnsaio de Imunoadsorção EnzimáticaImunoglobulina G/uso diagnósticoLeishmania infantum/isolamento & purificaçãoMesocricetusCamundongosCamundongos Endogâmicos BALB COrganismos Livres de Patógenos EspecíficosBaço/imunologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82352https://www.arca.fiocruz.br/bitstream/icict/10434/1/license.txtafbdf7d7a9bcf771a1cc7edf5f618413MD51ORIGINALMiranda GC Detection....pdfMiranda GC Detection....pdfapplication/pdf334379https://www.arca.fiocruz.br/bitstream/icict/10434/2/Miranda%20GC%20Detection....pdf09002450c41fecc94ac5dbcbeb1f1227MD52TEXTMiranda GC Detection....pdf.txtMiranda GC Detection....pdf.txtExtracted texttext/plain36246https://www.arca.fiocruz.br/bitstream/icict/10434/3/Miranda%20GC%20Detection....pdf.txt3b3e011b9ce5da6c8f18e13b08d07d79MD53icict/104342023-03-15 14:33:07.101oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:07Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Detection of parasite antigens in Leishmania infantum-infected spleen tissue by monoclonal antibody-, piezoelectric-based immunosensors.
title Detection of parasite antigens in Leishmania infantum-infected spleen tissue by monoclonal antibody-, piezoelectric-based immunosensors.
spellingShingle Detection of parasite antigens in Leishmania infantum-infected spleen tissue by monoclonal antibody-, piezoelectric-based immunosensors.
Miranda, Gustavo Cabral
Anticorpos Monoclonais/uso diagnóstico
Antígenos de Protozoários/isolamento & purificação
Leishmania infantum/imunologia
Leishmaniose Visceral/diagnóstico
Baço/parasitologia
Animais
Anticorpos Monoclonais/imunologia
Anticorpos Antiprotozoários/uso diagnóstico
Especificidade de Anticorpos
Técnicas Biossensoriais/métodos
Cricetinae
Ensaio de Imunoadsorção Enzimática
Imunoglobulina G/uso diagnóstico
Leishmania infantum/isolamento & purificação
Mesocricetus
Camundongos
Camundongos Endogâmicos BALB C
Organismos Livres de Patógenos Específicos
Baço/imunologia
title_short Detection of parasite antigens in Leishmania infantum-infected spleen tissue by monoclonal antibody-, piezoelectric-based immunosensors.
title_full Detection of parasite antigens in Leishmania infantum-infected spleen tissue by monoclonal antibody-, piezoelectric-based immunosensors.
title_fullStr Detection of parasite antigens in Leishmania infantum-infected spleen tissue by monoclonal antibody-, piezoelectric-based immunosensors.
title_full_unstemmed Detection of parasite antigens in Leishmania infantum-infected spleen tissue by monoclonal antibody-, piezoelectric-based immunosensors.
title_sort Detection of parasite antigens in Leishmania infantum-infected spleen tissue by monoclonal antibody-, piezoelectric-based immunosensors.
author Miranda, Gustavo Cabral
author_facet Miranda, Gustavo Cabral
Jesus, Joilson Ramos de
Oliveira, Pablo Rafael Silveira
Britto, G. S. G
Pontes-de-Carvalho, Lain Carlos
Dutra, R. F
Neves, Neuza Maria Alcântara
author_role author
author2 Jesus, Joilson Ramos de
Oliveira, Pablo Rafael Silveira
Britto, G. S. G
Pontes-de-Carvalho, Lain Carlos
Dutra, R. F
Neves, Neuza Maria Alcântara
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Miranda, Gustavo Cabral
Jesus, Joilson Ramos de
Oliveira, Pablo Rafael Silveira
Britto, G. S. G
Pontes-de-Carvalho, Lain Carlos
Dutra, R. F
Neves, Neuza Maria Alcântara
dc.subject.decs.pt_BR.fl_str_mv Anticorpos Monoclonais/uso diagnóstico
Antígenos de Protozoários/isolamento & purificação
Leishmania infantum/imunologia
Leishmaniose Visceral/diagnóstico
Baço/parasitologia
Animais
Anticorpos Monoclonais/imunologia
Anticorpos Antiprotozoários/uso diagnóstico
Especificidade de Anticorpos
Técnicas Biossensoriais/métodos
Cricetinae
Ensaio de Imunoadsorção Enzimática
Imunoglobulina G/uso diagnóstico
Leishmania infantum/isolamento & purificação
Mesocricetus
Camundongos
Camundongos Endogâmicos BALB C
Organismos Livres de Patógenos Específicos
Baço/imunologia
topic Anticorpos Monoclonais/uso diagnóstico
Antígenos de Protozoários/isolamento & purificação
Leishmania infantum/imunologia
Leishmaniose Visceral/diagnóstico
Baço/parasitologia
Animais
Anticorpos Monoclonais/imunologia
Anticorpos Antiprotozoários/uso diagnóstico
Especificidade de Anticorpos
Técnicas Biossensoriais/métodos
Cricetinae
Ensaio de Imunoadsorção Enzimática
Imunoglobulina G/uso diagnóstico
Leishmania infantum/isolamento & purificação
Mesocricetus
Camundongos
Camundongos Endogâmicos BALB C
Organismos Livres de Patógenos Específicos
Baço/imunologia
description Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-05-19T17:06:38Z
dc.date.available.fl_str_mv 2015-05-19T17:06:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MIRANDA, G. C. et al. Detection of parasite antigens in Leishmania infantum-infected spleen tissue by monoclonal antibody-, piezoelectric-based immunosensors. Journal of Parasitology, v. 100, n. 1, p. 73-78, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10434
dc.identifier.issn.none.fl_str_mv 1937-2345
dc.identifier.doi.none.fl_str_mv dx.doi.org/10.1645/GE-3052.1
identifier_str_mv MIRANDA, G. C. et al. Detection of parasite antigens in Leishmania infantum-infected spleen tissue by monoclonal antibody-, piezoelectric-based immunosensors. Journal of Parasitology, v. 100, n. 1, p. 73-78, 2014.
1937-2345
dx.doi.org/10.1645/GE-3052.1
url https://www.arca.fiocruz.br/handle/icict/10434
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society of Parasitologists
publisher.none.fl_str_mv American Society of Parasitologists
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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