COVID-2019: The role of the nsp2 and nsp3 in its pathogenesis

Detalhes bibliográficos
Autor(a) principal: Angeletti, Silvia
Data de Publicação: 2020
Outros Autores: Benvenuto, Domenico, Bianchi, Martina, Giovanetti, Marta, Pascarella, Stefano, Ciccozzi, Massimo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/40392
Resumo: Devido a pandemia mundial do COVID-19, o artigo da revista foi liberado em acesso aberto durante o ano de 2020, visando divulgação e disseminação.
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spelling Angeletti, SilviaBenvenuto, DomenicoBianchi, MartinaGiovanetti, MartaPascarella, StefanoCiccozzi, Massimo2020-03-18T19:01:29Z2020-03-18T19:01:29Z2020ANGELETTI, Silvia et al. COVID‐2019: The role of the nsp2 and nsp3inits pathogenesis. Journal of Medical Virology, p. 1-5, 2020.0146-6615https://www.arca.fiocruz.br/handle/icict/4039210.1002/jmv.257191096-9071Devido a pandemia mundial do COVID-19, o artigo da revista foi liberado em acesso aberto durante o ano de 2020, visando divulgação e disseminação.Em decorrência da pandemia decretada pela OMS em 2020, o artigo encontra-se em acesso aberto.University Campus Bio‐Medico of Rome. Unit of Clinical Laboratory Science. Rome, Italy.University Campus Bio‐Medico of Rome. Unit of Medical Statistics and Molecular Epidemiology. Rome, Italy.University of Rome “La Sapienza”. Department of Biochemical Sciences "A. Rossi Fanelli". Rome, Italy.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil.University of Rome “La Sapienza”. Department of Biochemical Sciences "A. Rossi Fanelli". Rome, Italy.University Campus Bio‐Medico of Rome. Unit of Medical Statistics and Molecular Epidemiology. Rome, Italy.Last December 2019, a new virus, named novel Coronavirus (COVID-2019) causing many cases of severe pneumonia was reported in Wuhan, China. The virus knowledge is limited and especially about COVID-2019 pathogenesis. The Open Reading Frame 1ab (ORF1ab) of COVID-2019 has been analyzed to evidence the presence of mutation caused by selective pressure on the virus. For selective pressure analysis fast-unconstrained Bayesian approximation (FUBAR) was used. Homology modelling has been performed by SwissModel and HHPred servers. The presence of transmembrane helical segments in Coronavirus ORF1ab non structural protein 2 (nsp2) and nsp3 was tested by TMHMM, MEMSAT, and MEMPACK tools. Three-dimensional structures have been analyzed and displayed using PyMOL. FUBAR analysis revealed the presence of potential sites under positive selective pressure (P < .05). Position 723 in the COVID-2019 has a serine instead a glycine residue, while at aminoacidic position 1010 a proline instead an isoleucine. Significant (P < .05) pervasive negative selection in 2416 sites (55%) was found. The positive selective pressure could account for some clinical features of this virus compared with severe acute respiratory syndrome (SARS) and Bat SARS-like CoV. The stabilizing mutation falling in the endosome-associated-protein-like domain of the nsp2 protein could account for COVID-2019 high ability of contagious, while the destabilizing mutation in nsp3 proteins could suggest a potential mechanism differentiating COVID-2019 from SARS. These data could be helpful for further investigation aimed to identify potential therapeutic targets or vaccine strategy, especially in the actual moment when the epidemic is ongoing and the scientific community is trying to enrich knowledge about this new viral pathogen.engWileyEpidemiologiaPandemiaInfecçãoPatogêneseAnálise de interação proteína-proteínaMétodos de pesquisa e análiseCOVID-19CoronavírusEpidemiologyInfectionPandemicsPathogeneseisProtein-protein interaction analysisResearch and analysis methodsCOVID-2019: The role of the nsp2 and nsp3 in its pathogenesisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv COVID-2019: The role of the nsp2 and nsp3 in its pathogenesis
title COVID-2019: The role of the nsp2 and nsp3 in its pathogenesis
spellingShingle COVID-2019: The role of the nsp2 and nsp3 in its pathogenesis
Angeletti, Silvia
Epidemiologia
Pandemia
Infecção
Patogênese
Análise de interação proteína-proteína
Métodos de pesquisa e análise
COVID-19
Coronavírus
Epidemiology
Infection
Pandemics
Pathogeneseis
Protein-protein interaction analysis
Research and analysis methods
title_short COVID-2019: The role of the nsp2 and nsp3 in its pathogenesis
title_full COVID-2019: The role of the nsp2 and nsp3 in its pathogenesis
title_fullStr COVID-2019: The role of the nsp2 and nsp3 in its pathogenesis
title_full_unstemmed COVID-2019: The role of the nsp2 and nsp3 in its pathogenesis
title_sort COVID-2019: The role of the nsp2 and nsp3 in its pathogenesis
author Angeletti, Silvia
author_facet Angeletti, Silvia
Benvenuto, Domenico
Bianchi, Martina
Giovanetti, Marta
Pascarella, Stefano
Ciccozzi, Massimo
author_role author
author2 Benvenuto, Domenico
Bianchi, Martina
Giovanetti, Marta
Pascarella, Stefano
Ciccozzi, Massimo
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Angeletti, Silvia
Benvenuto, Domenico
Bianchi, Martina
Giovanetti, Marta
Pascarella, Stefano
Ciccozzi, Massimo
dc.subject.other.pt_BR.fl_str_mv Epidemiologia
Pandemia
Infecção
Patogênese
Análise de interação proteína-proteína
Métodos de pesquisa e análise
COVID-19
Coronavírus
topic Epidemiologia
Pandemia
Infecção
Patogênese
Análise de interação proteína-proteína
Métodos de pesquisa e análise
COVID-19
Coronavírus
Epidemiology
Infection
Pandemics
Pathogeneseis
Protein-protein interaction analysis
Research and analysis methods
dc.subject.en.pt_BR.fl_str_mv Epidemiology
Infection
Pandemics
Pathogeneseis
Protein-protein interaction analysis
Research and analysis methods
description Devido a pandemia mundial do COVID-19, o artigo da revista foi liberado em acesso aberto durante o ano de 2020, visando divulgação e disseminação.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-03-18T19:01:29Z
dc.date.available.fl_str_mv 2020-03-18T19:01:29Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ANGELETTI, Silvia et al. COVID‐2019: The role of the nsp2 and nsp3inits pathogenesis. Journal of Medical Virology, p. 1-5, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/40392
dc.identifier.issn.pt_BR.fl_str_mv 0146-6615
dc.identifier.doi.none.fl_str_mv 10.1002/jmv.25719
dc.identifier.eissn.none.fl_str_mv 1096-9071
identifier_str_mv ANGELETTI, Silvia et al. COVID‐2019: The role of the nsp2 and nsp3inits pathogenesis. Journal of Medical Virology, p. 1-5, 2020.
0146-6615
10.1002/jmv.25719
1096-9071
url https://www.arca.fiocruz.br/handle/icict/40392
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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