Gain of function in Mycobacterium bovis BCG Moreau due to loss of a transcriptional repressor

Detalhes bibliográficos
Autor(a) principal: Maia, Renata Monteiro
Data de Publicação: 2018
Outros Autores: Correa, Paloma Rezende, Vaconcelos, Periela da Silva Souza, Pinho, Rosa Teixeira de, Lima, Leila Mendonça
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/29877
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.
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spelling Maia, Renata MonteiroCorrea, Paloma RezendeVaconcelos, Periela da Silva SouzaPinho, Rosa Teixeira deLima, Leila Mendonça2018-11-06T10:39:30Z2018-11-06T10:39:30Z2018MAIA, Renata Monteiro; et al. Gain of function in Mycobacterium bovis BCG Moreau due to loss of a transcriptional repressor. Mem Inst Oswaldo Cruz, v.113, n.11, e180267, 2018.0074-0276https://www.arca.fiocruz.br/handle/icict/298771678-8060engFundação Oswaldo Cruz. Instituto Oswaldo CruzTuberculoseBacille Calmette-GuérinVacina BCGCélulas THP-1BCG Moreaurv3405cRV3406THP-1 cellstuberculosisGain of function in Mycobacterium bovis BCG Moreau due to loss of a transcriptional repressorinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.The Bacille Calmette-Guérin (BCG) vaccine comprises a family of genetically different strains derived by the loss of genomic regions (RDs) and other mutations. In BCG Moreau, loss of RD16 inactivates rv3405c*, encoding a transcriptional repressor that negatively regulates the expression of Rv3406, an alkyl sulfatase. To evaluate the impact of this loss on the BCG and host cell viability and the cytokine profile, THP-1 cells were infected with BCG Moreau (harbouring the empty vector) and a complemented strain carrying a functional copy of rv3405c. Viability of the host cells and bacteria as well as the pattern of cytokine secretion were evaluated. Our results show that the viability of BCG Moreau is higher than that of the complemented strain in an axenic medium, suggesting a possible functional gain associated with the constitutive expression of Rv3406. Viability of the host cells did not vary significantly between recombinant strains, but differences in the profiles of the cytokine secretion (IL-1β and IL-6) were observed. Our results suggest an example of a functional gain due to gene loss contributing to the elucidation of the impact of RD16 on the physiology of BCG Moreau.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/29877/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALrenata_maia_etal_IOC_2018.;pdf.pdfrenata_maia_etal_IOC_2018.;pdf.pdfapplication/pdf498621https://www.arca.fiocruz.br/bitstream/icict/29877/2/renata_maia_etal_IOC_2018.%3bpdf.pdfe921715f0847b10d3fdf4147de032421MD52TEXTrenata_maia_etal_IOC_2018.;pdf.pdf.txtrenata_maia_etal_IOC_2018.;pdf.pdf.txtExtracted texttext/plain25551https://www.arca.fiocruz.br/bitstream/icict/29877/3/renata_maia_etal_IOC_2018.%3bpdf.pdf.txt55a62ca6ee28193e86ca417fe4e3f287MD53icict/298772018-11-07 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dc.title.pt_BR.fl_str_mv Gain of function in Mycobacterium bovis BCG Moreau due to loss of a transcriptional repressor
title Gain of function in Mycobacterium bovis BCG Moreau due to loss of a transcriptional repressor
spellingShingle Gain of function in Mycobacterium bovis BCG Moreau due to loss of a transcriptional repressor
Maia, Renata Monteiro
Tuberculose
Bacille Calmette-Guérin
Vacina BCG
Células THP-1
BCG Moreau
rv3405c
RV3406
THP-1 cells
tuberculosis
title_short Gain of function in Mycobacterium bovis BCG Moreau due to loss of a transcriptional repressor
title_full Gain of function in Mycobacterium bovis BCG Moreau due to loss of a transcriptional repressor
title_fullStr Gain of function in Mycobacterium bovis BCG Moreau due to loss of a transcriptional repressor
title_full_unstemmed Gain of function in Mycobacterium bovis BCG Moreau due to loss of a transcriptional repressor
title_sort Gain of function in Mycobacterium bovis BCG Moreau due to loss of a transcriptional repressor
author Maia, Renata Monteiro
author_facet Maia, Renata Monteiro
Correa, Paloma Rezende
Vaconcelos, Periela da Silva Souza
Pinho, Rosa Teixeira de
Lima, Leila Mendonça
author_role author
author2 Correa, Paloma Rezende
Vaconcelos, Periela da Silva Souza
Pinho, Rosa Teixeira de
Lima, Leila Mendonça
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Maia, Renata Monteiro
Correa, Paloma Rezende
Vaconcelos, Periela da Silva Souza
Pinho, Rosa Teixeira de
Lima, Leila Mendonça
dc.subject.other.pt_BR.fl_str_mv Tuberculose
Bacille Calmette-Guérin
Vacina BCG
Células THP-1
topic Tuberculose
Bacille Calmette-Guérin
Vacina BCG
Células THP-1
BCG Moreau
rv3405c
RV3406
THP-1 cells
tuberculosis
dc.subject.en.pt_BR.fl_str_mv BCG Moreau
rv3405c
RV3406
THP-1 cells
tuberculosis
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-11-06T10:39:30Z
dc.date.available.fl_str_mv 2018-11-06T10:39:30Z
dc.date.issued.fl_str_mv 2018
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MAIA, Renata Monteiro; et al. Gain of function in Mycobacterium bovis BCG Moreau due to loss of a transcriptional repressor. Mem Inst Oswaldo Cruz, v.113, n.11, e180267, 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/29877
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv MAIA, Renata Monteiro; et al. Gain of function in Mycobacterium bovis BCG Moreau due to loss of a transcriptional repressor. Mem Inst Oswaldo Cruz, v.113, n.11, e180267, 2018.
0074-0276
1678-8060
url https://www.arca.fiocruz.br/handle/icict/29877
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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