Viability and molecular authentication of Coccidioides immitis strains from Culture Collection of the Instituto Oswaldo Cruz, Rio de Janeiro, Brazil

Detalhes bibliográficos
Autor(a) principal: Bezerra, Claudia C. F.
Data de Publicação: 2013
Outros Autores: Renata F. de, Lima, Márcia S., Lazera, Bodo, Wanke, Cintia M., Borba
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/38921
Resumo: Vinte cepas de Coccidioides immitis foram avaliadas. Cinco das 20 cepas preservadas sob óleo mineral mantiveram-se viáveis, todas as 5 subculturas preservadas em água permaneceram viáveis e nenhuma das 13 subculturas mantidas em solo foi viável. Um produto de PCR de 519 pb do gene csa confirmou a identidade das cepas.
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spelling Bezerra, Claudia C. F.Renata F. de, LimaMárcia S., LazeraBodo, WankeCintia M., Borba2020-01-01T17:17:53Z2020-01-01T17:17:53Z2013BEZERRA, Claudia C. F. et al. Viability and molecular authentication of Coccidioides immitis strains from Culture Collection of the Instituto Oswaldo Cruz, Rio de Janeiro, Brazil. Revista da Sociedade Brasileira de Medicina Tropical, Uberaba, v. 39, n. 3, p. 241-244, maio/jun. 2006.0037-8682https://www.arca.fiocruz.br/handle/icict/3892110.1590/s0036-466520130001000021678-9849Vinte cepas de Coccidioides immitis foram avaliadas. Cinco das 20 cepas preservadas sob óleo mineral mantiveram-se viáveis, todas as 5 subculturas preservadas em água permaneceram viáveis e nenhuma das 13 subculturas mantidas em solo foi viável. Um produto de PCR de 519 pb do gene csa confirmou a identidade das cepas.Twenty Coccidioides immitis strains were evaluated. Only 5 of the 20 strains kept under mineral oil maintained their viability while all 5 subcultures preserved in water remained viable and none of the 13 subcultures kept in soil were viable. A 519 bp PCR product from the csa gene confirmed the identity of the strains.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Servico de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Servico de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Servico de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Micologia. 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dc.title.pt_BR.fl_str_mv Viability and molecular authentication of Coccidioides immitis strains from Culture Collection of the Instituto Oswaldo Cruz, Rio de Janeiro, Brazil
dc.title.alternative.pt_BR.fl_str_mv Viabilidade e autenticação molecular de cepas de Coccidioides immitis da Coleção de Culturas do Instituto Oswaldo Cruz, Rio de Janeiro, Brasil
title Viability and molecular authentication of Coccidioides immitis strains from Culture Collection of the Instituto Oswaldo Cruz, Rio de Janeiro, Brazil
spellingShingle Viability and molecular authentication of Coccidioides immitis strains from Culture Collection of the Instituto Oswaldo Cruz, Rio de Janeiro, Brazil
Bezerra, Claudia C. F.
Coccidioides immitis
Preservação por longos períodos
PCR
Coccidioides immitis
PCR
Long-term preservation
title_short Viability and molecular authentication of Coccidioides immitis strains from Culture Collection of the Instituto Oswaldo Cruz, Rio de Janeiro, Brazil
title_full Viability and molecular authentication of Coccidioides immitis strains from Culture Collection of the Instituto Oswaldo Cruz, Rio de Janeiro, Brazil
title_fullStr Viability and molecular authentication of Coccidioides immitis strains from Culture Collection of the Instituto Oswaldo Cruz, Rio de Janeiro, Brazil
title_full_unstemmed Viability and molecular authentication of Coccidioides immitis strains from Culture Collection of the Instituto Oswaldo Cruz, Rio de Janeiro, Brazil
title_sort Viability and molecular authentication of Coccidioides immitis strains from Culture Collection of the Instituto Oswaldo Cruz, Rio de Janeiro, Brazil
author Bezerra, Claudia C. F.
author_facet Bezerra, Claudia C. F.
Renata F. de, Lima
Márcia S., Lazera
Bodo, Wanke
Cintia M., Borba
author_role author
author2 Renata F. de, Lima
Márcia S., Lazera
Bodo, Wanke
Cintia M., Borba
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Bezerra, Claudia C. F.
Renata F. de, Lima
Márcia S., Lazera
Bodo, Wanke
Cintia M., Borba
dc.subject.other.pt_BR.fl_str_mv Coccidioides immitis
Preservação por longos períodos
PCR
topic Coccidioides immitis
Preservação por longos períodos
PCR
Coccidioides immitis
PCR
Long-term preservation
dc.subject.en.pt_BR.fl_str_mv Coccidioides immitis
PCR
Long-term preservation
description Vinte cepas de Coccidioides immitis foram avaliadas. Cinco das 20 cepas preservadas sob óleo mineral mantiveram-se viáveis, todas as 5 subculturas preservadas em água permaneceram viáveis e nenhuma das 13 subculturas mantidas em solo foi viável. Um produto de PCR de 519 pb do gene csa confirmou a identidade das cepas.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2020-01-01T17:17:53Z
dc.date.available.fl_str_mv 2020-01-01T17:17:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BEZERRA, Claudia C. F. et al. Viability and molecular authentication of Coccidioides immitis strains from Culture Collection of the Instituto Oswaldo Cruz, Rio de Janeiro, Brazil. Revista da Sociedade Brasileira de Medicina Tropical, Uberaba, v. 39, n. 3, p. 241-244, maio/jun. 2006.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/38921
dc.identifier.issn.pt_BR.fl_str_mv 0037-8682
dc.identifier.doi.none.fl_str_mv 10.1590/s0036-46652013000100002
dc.identifier.eissn.none.fl_str_mv 1678-9849
identifier_str_mv BEZERRA, Claudia C. F. et al. Viability and molecular authentication of Coccidioides immitis strains from Culture Collection of the Instituto Oswaldo Cruz, Rio de Janeiro, Brazil. Revista da Sociedade Brasileira de Medicina Tropical, Uberaba, v. 39, n. 3, p. 241-244, maio/jun. 2006.
0037-8682
10.1590/s0036-46652013000100002
1678-9849
url https://www.arca.fiocruz.br/handle/icict/38921
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical
publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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