Rapid translocation of polarized MDCK cell monolayers by Leptospira interrogans, an invasive but nonintracellular pathogen
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2002 |
| Outros Autores: | , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/7566 |
Resumo: | Division of Infectious Diseases and Immunity. School of Public Health. Berkeley, California |
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Barocchi, Michele AKo, Albert IcksangReis, Mitermayer Galvão dosMcDonald, Kent LRiley, Lee Woodland2014-05-02T13:17:56Z2014-05-02T13:17:56Z2002BAROCCHI, M. A. et al. Rapid translocation of polarized MDCK cell monolayers by Leptospira interrogans, an invasive but nonintracellular pathogen. Infection and Immunity, v. 70, n. 12, p. 6926-6932, 2002.0019-9567https://www.arca.fiocruz.br/handle/icict/756610.1128/IAI.70.12.6926–6932.2002engAmerican Society for MicrobiologyRapid translocation of polarized MDCK cell monolayers by Leptospira interrogans, an invasive but nonintracellular pathogeninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleDivision of Infectious Diseases and Immunity. School of Public Health. Berkeley, CaliforniaFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Division of International Medicine and Infectious Diseases. Weil Medical College of Cornell University. New York, NYUC Berkeley Electron Microscopy Laboratory. University of California. Berkeley, Berkeley, CaliforniaDivision of Infectious Diseases and Immunity. School of Public Health. Berkeley, CaliforniaPathogenic spirochetes of the genus Leptospira are a major cause of human zoonotic infectious disease worldwide. After gaining entry through the skin, the organism causes disease by hematogenously disseminating to multiple organs. The mechanism by which it penetrates the mammalian cell barriers to disseminate is not well understood. In this study, we used a low-passage-number isolate of Leptospira interrogans to elucidate the invasive potential of this spirochete. Quantification of bacteria by dark-field microscopy revealed that pathogenic spirochetes were able to translocate through polarized MDCK cell monolayers at a rate significantly greater than that of nonpathogenic Leptospira or a recognized invasive bacterial pathogen, Salmonella. In contrast to Salmonella, L. interrogans did not alter transepithelial electrical resistance during cell translocation. Both transmission and scanning electron microscopy revealed tight association of the extracellular spirochetes with the host cell plasma membrane, without membrane perturbations suggestive of cytoskeletal rearrangement. Spirochetes were not observed within intercellular junctions or membrane-bound compartments inside cells. They were found within the cytoplasm of only 8% of the counted cells. These results indicate that Leptospira is an invasive but not a facultative intracellular organism. We propose that the rapid translocation of mammalian cells by pathogenic Leptospira is a mechanism designed to evade killing by host cells that permits the organism to quickly reach the bloodstream and disseminate to multiple organs.Polaridade CelularCélulas Epiteliais/microbiologiaLeptospira interrogans/patogenicidadeAnimaisLinhagem CelularCãesCondutividade ElétricaCélulas Epiteliais/ultraestruturaInterações Hospedeiro-ParasitaJunções Intercelulares/ultraestruturaRim/citologiaRim/microbiologiaMicroscopia Eletrônica de VarreduraMicrovilosidades/ultraestruturaFatores de TempoVirulênciainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALBarocchi MA Rapid Translocation....pdfBarocchi MA Rapid Translocation....pdfapplication/pdf1163063https://www.arca.fiocruz.br/bitstream/icict/7566/1/Barocchi%20MA%20Rapid%20Translocation....pdf395a6a2974920883822ea4e275259bb4MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7566/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTBarocchi MA Rapid Translocation....pdf.txtBarocchi MA Rapid Translocation....pdf.txtExtracted texttext/plain33013https://www.arca.fiocruz.br/bitstream/icict/7566/5/Barocchi%20MA%20Rapid%20Translocation....pdf.txt8998b0953ca424a245a9915dbf506e64MD55THUMBNAILBarocchi MA Rapid Translocation....pdf.jpgBarocchi MA Rapid Translocation....pdf.jpgGenerated Thumbnailimage/jpeg1855https://www.arca.fiocruz.br/bitstream/icict/7566/4/Barocchi%20MA%20Rapid%20Translocation....pdf.jpg1126f5da1537c8f619c149d30508460bMD54icict/75662023-03-15 14:33:12.123oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:12Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Rapid translocation of polarized MDCK cell monolayers by Leptospira interrogans, an invasive but nonintracellular pathogen |
| title |
Rapid translocation of polarized MDCK cell monolayers by Leptospira interrogans, an invasive but nonintracellular pathogen |
| spellingShingle |
Rapid translocation of polarized MDCK cell monolayers by Leptospira interrogans, an invasive but nonintracellular pathogen Barocchi, Michele A Polaridade Celular Células Epiteliais/microbiologia Leptospira interrogans/patogenicidade Animais Linhagem Celular Cães Condutividade Elétrica Células Epiteliais/ultraestrutura Interações Hospedeiro-Parasita Junções Intercelulares/ultraestrutura Rim/citologia Rim/microbiologia Microscopia Eletrônica de Varredura Microvilosidades/ultraestrutura Fatores de Tempo Virulência |
| title_short |
Rapid translocation of polarized MDCK cell monolayers by Leptospira interrogans, an invasive but nonintracellular pathogen |
| title_full |
Rapid translocation of polarized MDCK cell monolayers by Leptospira interrogans, an invasive but nonintracellular pathogen |
| title_fullStr |
Rapid translocation of polarized MDCK cell monolayers by Leptospira interrogans, an invasive but nonintracellular pathogen |
| title_full_unstemmed |
Rapid translocation of polarized MDCK cell monolayers by Leptospira interrogans, an invasive but nonintracellular pathogen |
| title_sort |
Rapid translocation of polarized MDCK cell monolayers by Leptospira interrogans, an invasive but nonintracellular pathogen |
| author |
Barocchi, Michele A |
| author_facet |
Barocchi, Michele A Ko, Albert Icksang Reis, Mitermayer Galvão dos McDonald, Kent L Riley, Lee Woodland |
| author_role |
author |
| author2 |
Ko, Albert Icksang Reis, Mitermayer Galvão dos McDonald, Kent L Riley, Lee Woodland |
| author2_role |
author author author author |
| dc.contributor.author.fl_str_mv |
Barocchi, Michele A Ko, Albert Icksang Reis, Mitermayer Galvão dos McDonald, Kent L Riley, Lee Woodland |
| dc.subject.decs.pt_BR.fl_str_mv |
Polaridade Celular Células Epiteliais/microbiologia Leptospira interrogans/patogenicidade Animais Linhagem Celular Cães Condutividade Elétrica Células Epiteliais/ultraestrutura Interações Hospedeiro-Parasita Junções Intercelulares/ultraestrutura Rim/citologia Rim/microbiologia Microscopia Eletrônica de Varredura Microvilosidades/ultraestrutura Fatores de Tempo Virulência |
| topic |
Polaridade Celular Células Epiteliais/microbiologia Leptospira interrogans/patogenicidade Animais Linhagem Celular Cães Condutividade Elétrica Células Epiteliais/ultraestrutura Interações Hospedeiro-Parasita Junções Intercelulares/ultraestrutura Rim/citologia Rim/microbiologia Microscopia Eletrônica de Varredura Microvilosidades/ultraestrutura Fatores de Tempo Virulência |
| description |
Division of Infectious Diseases and Immunity. School of Public Health. Berkeley, California |
| publishDate |
2002 |
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2002 |
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2014-05-02T13:17:56Z |
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2014-05-02T13:17:56Z |
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info:eu-repo/semantics/article |
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article |
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| dc.identifier.citation.fl_str_mv |
BAROCCHI, M. A. et al. Rapid translocation of polarized MDCK cell monolayers by Leptospira interrogans, an invasive but nonintracellular pathogen. Infection and Immunity, v. 70, n. 12, p. 6926-6932, 2002. |
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0019-9567 |
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10.1128/IAI.70.12.6926–6932.2002 |
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BAROCCHI, M. A. et al. Rapid translocation of polarized MDCK cell monolayers by Leptospira interrogans, an invasive but nonintracellular pathogen. Infection and Immunity, v. 70, n. 12, p. 6926-6932, 2002. 0019-9567 10.1128/IAI.70.12.6926–6932.2002 |
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American Society for Microbiology |
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American Society for Microbiology |
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