Effects of High Glucose Concentrations on the Endothelial Function of the Renal Microcirculation of Rabbits
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/33540 |
Resumo: | Objective – To assess the acute effects of high glucose concentrations on vascular reactivity in the isolated non diabetic rabbit kidney. Methods – Rabbits were anaesthetized for isolation of the kidneys. Renal arteries and veins were cannulated for perfusion with Krebs-Henselleit solution and measurement of perfusion pressure. After 3 hours of perfusion with glucose 5,5 mM (control ) and 15 mM, the circulation was submitted to sub maximal precontraction (80% of maximal response) trough continuous infusion of noradrenaline 10 mM. Vascular reactivity was then assessed trough dose–responses curves with endothelium-dependent (acetylcholine) and independent (sodium nitroprusside) vasodilators. The influence of hyperosmolarity was analyzed with perfusion with mannitol 15mM. Results – A significant reduction in the endotheliumdependent vasodilation in glucose 15mM group was observed compared to that in control, but there was no difference in endothelium-independent vasodilation. After perfusion with mannitol 15 mM, a less expressive reduction in endothelium-dependent vasodilation was observed, only reaching significance in regard to the greatest dose of acetylcholine. Conclusions – High levels of glucose similar to those found in diabetic patients in the postprandial period can cause significant acute changes in renal vascular reactivity rabbits. In diabetic patients these effects may also occur and contribute to diabetes vascular disease. |
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Affonso, Filipe de SouzaCailleaux, SolangePinto, Leonardo Felipe CorrêaGomes, Marilia de BritoTibiriçá, Eduardo2019-06-18T13:24:50Z2019-06-18T13:24:50Z2003AFFONSO, Filipe de Souza et al. Effects of High Glucose Concentrations on the Endothelial Function of the Renal Microcirculation of Rabbits. Arquivos Brasileiros de Cardiologia, v. 81, n. 1, p. 161-165, 2003.0066-782Xhttps://www.arca.fiocruz.br/handle/icict/3354010.1590/S0066-782X20030010000051678-4170Objective – To assess the acute effects of high glucose concentrations on vascular reactivity in the isolated non diabetic rabbit kidney. Methods – Rabbits were anaesthetized for isolation of the kidneys. Renal arteries and veins were cannulated for perfusion with Krebs-Henselleit solution and measurement of perfusion pressure. After 3 hours of perfusion with glucose 5,5 mM (control ) and 15 mM, the circulation was submitted to sub maximal precontraction (80% of maximal response) trough continuous infusion of noradrenaline 10 mM. Vascular reactivity was then assessed trough dose–responses curves with endothelium-dependent (acetylcholine) and independent (sodium nitroprusside) vasodilators. The influence of hyperosmolarity was analyzed with perfusion with mannitol 15mM. Results – A significant reduction in the endotheliumdependent vasodilation in glucose 15mM group was observed compared to that in control, but there was no difference in endothelium-independent vasodilation. After perfusion with mannitol 15 mM, a less expressive reduction in endothelium-dependent vasodilation was observed, only reaching significance in regard to the greatest dose of acetylcholine. Conclusions – High levels of glucose similar to those found in diabetic patients in the postprandial period can cause significant acute changes in renal vascular reactivity rabbits. In diabetic patients these effects may also occur and contribute to diabetes vascular disease.Universidade do Estado do Rio de Janeiro. Hospital Universitário Pedro Ernesto. Laboratório de Farmacologia Neuro-Cardiovascular. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Hospital Universitário Pedro Ernesto. Laboratório de Farmacologia Neuro-Cardiovascular. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Hospital Universitário Pedro Ernesto. Laboratório de Farmacologia Neuro-Cardiovascular. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Hospital Universitário Pedro Ernesto. Laboratório de Farmacologia Neuro-Cardiovascular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Farmacologia Neuro Cardiovascular. Rio de Janeiro, RJ. Brasil.engSociedade Brasileira de CardiologiaDisfunção endotelialHiperglicemiaCirculação RenalEndothelial dysfunctionHyperglycemiaRenal circulationEffects of High Glucose Concentrations on the Endothelial Function of the Renal Microcirculation of Rabbitsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/33540/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALEduardoTibirca_FilipeSAfonso_etal_IOC_2003.pdfEduardoTibirca_FilipeSAfonso_etal_IOC_2003.pdfapplication/pdf54454https://www.arca.fiocruz.br/bitstream/icict/33540/2/EduardoTibirca_FilipeSAfonso_etal_IOC_2003.pdf8fac63506f2c3be7ad182cfd91c1dae7MD52TEXTEduardoTibirca_FilipeSAfonso_etal_IOC_2003.pdf.txtEduardoTibirca_FilipeSAfonso_etal_IOC_2003.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv |
Effects of High Glucose Concentrations on the Endothelial Function of the Renal Microcirculation of Rabbits |
title |
Effects of High Glucose Concentrations on the Endothelial Function of the Renal Microcirculation of Rabbits |
spellingShingle |
Effects of High Glucose Concentrations on the Endothelial Function of the Renal Microcirculation of Rabbits Affonso, Filipe de Souza Disfunção endotelial Hiperglicemia Circulação Renal Endothelial dysfunction Hyperglycemia Renal circulation |
title_short |
Effects of High Glucose Concentrations on the Endothelial Function of the Renal Microcirculation of Rabbits |
title_full |
Effects of High Glucose Concentrations on the Endothelial Function of the Renal Microcirculation of Rabbits |
title_fullStr |
Effects of High Glucose Concentrations on the Endothelial Function of the Renal Microcirculation of Rabbits |
title_full_unstemmed |
Effects of High Glucose Concentrations on the Endothelial Function of the Renal Microcirculation of Rabbits |
title_sort |
Effects of High Glucose Concentrations on the Endothelial Function of the Renal Microcirculation of Rabbits |
author |
Affonso, Filipe de Souza |
author_facet |
Affonso, Filipe de Souza Cailleaux, Solange Pinto, Leonardo Felipe Corrêa Gomes, Marilia de Brito Tibiriçá, Eduardo |
author_role |
author |
author2 |
Cailleaux, Solange Pinto, Leonardo Felipe Corrêa Gomes, Marilia de Brito Tibiriçá, Eduardo |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Affonso, Filipe de Souza Cailleaux, Solange Pinto, Leonardo Felipe Corrêa Gomes, Marilia de Brito Tibiriçá, Eduardo |
dc.subject.other.pt_BR.fl_str_mv |
Disfunção endotelial Hiperglicemia Circulação Renal |
topic |
Disfunção endotelial Hiperglicemia Circulação Renal Endothelial dysfunction Hyperglycemia Renal circulation |
dc.subject.en.pt_BR.fl_str_mv |
Endothelial dysfunction Hyperglycemia Renal circulation |
description |
Objective – To assess the acute effects of high glucose concentrations on vascular reactivity in the isolated non diabetic rabbit kidney. Methods – Rabbits were anaesthetized for isolation of the kidneys. Renal arteries and veins were cannulated for perfusion with Krebs-Henselleit solution and measurement of perfusion pressure. After 3 hours of perfusion with glucose 5,5 mM (control ) and 15 mM, the circulation was submitted to sub maximal precontraction (80% of maximal response) trough continuous infusion of noradrenaline 10 mM. Vascular reactivity was then assessed trough dose–responses curves with endothelium-dependent (acetylcholine) and independent (sodium nitroprusside) vasodilators. The influence of hyperosmolarity was analyzed with perfusion with mannitol 15mM. Results – A significant reduction in the endotheliumdependent vasodilation in glucose 15mM group was observed compared to that in control, but there was no difference in endothelium-independent vasodilation. After perfusion with mannitol 15 mM, a less expressive reduction in endothelium-dependent vasodilation was observed, only reaching significance in regard to the greatest dose of acetylcholine. Conclusions – High levels of glucose similar to those found in diabetic patients in the postprandial period can cause significant acute changes in renal vascular reactivity rabbits. In diabetic patients these effects may also occur and contribute to diabetes vascular disease. |
publishDate |
2003 |
dc.date.issued.fl_str_mv |
2003 |
dc.date.accessioned.fl_str_mv |
2019-06-18T13:24:50Z |
dc.date.available.fl_str_mv |
2019-06-18T13:24:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
AFFONSO, Filipe de Souza et al. Effects of High Glucose Concentrations on the Endothelial Function of the Renal Microcirculation of Rabbits. Arquivos Brasileiros de Cardiologia, v. 81, n. 1, p. 161-165, 2003. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/33540 |
dc.identifier.issn.pt_BR.fl_str_mv |
0066-782X |
dc.identifier.doi.pt_BR.fl_str_mv |
10.1590/S0066-782X2003001000005 |
dc.identifier.eissn.none.fl_str_mv |
1678-4170 |
identifier_str_mv |
AFFONSO, Filipe de Souza et al. Effects of High Glucose Concentrations on the Endothelial Function of the Renal Microcirculation of Rabbits. Arquivos Brasileiros de Cardiologia, v. 81, n. 1, p. 161-165, 2003. 0066-782X 10.1590/S0066-782X2003001000005 1678-4170 |
url |
https://www.arca.fiocruz.br/handle/icict/33540 |
dc.language.iso.fl_str_mv |
eng |
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eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Cardiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Cardiologia |
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reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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Repositório Institucional da FIOCRUZ (ARCA) |
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